Effects of two juvenile hormone analogs on hemolymph and fat-body metabolites of the eastern spruce budworm, Choristoneura fumiferana (Clemens) (Lepidoptera: Tortricidae)

1993 ◽  
Vol 71 (6) ◽  
pp. 1169-1174 ◽  
Author(s):  
Hemendra Mulye ◽  
Roger Gordon
Keyword(s):  
Juvenile Hormone ◽  
Soluble Protein ◽  
Fat Body ◽  
Choristoneura Fumiferana ◽  
Experimental Period ◽  
Protein Levels ◽  
Carbohydrate Concentration ◽  
Eastern Spruce Budworm ◽  
Fat Bodies ◽  
Hormone Analogs

Early sixth instar larvae of Choristoneura fumiferana were treated with LD50 doses of the juvenile hormone analogs fenoxycarb and methoprene. Fenoxycarb caused an initial decrease, then an increase, in hemolymph carbohydrate concentration; fat-body glycogen was depleted initially, then increased in concentration (cf. controls) toward the end of the 6-day experimental period. Methoprene caused an opposite effect on hemolymph carbohydrates, but a similar effect (cf. fenoxycarb) on fat-body glycogen. Protein concentrations in the hemolymph of fenoxycarb-treated and methoprene-treated insects were lower than those of controls. The concentration of fat-body soluble protein of fenoxycarb-treated insects was initially lower, then higher, than that of controls. Methoprene did not alter soluble protein levels in the fat bodies. Lipids in the hemolymph of fenoxycarb-treated insects were depleted (cf. controls) throughout the experimental period; in the fat bodies, lipid concentration was elevated initially, then lowered. In methoprene-treated insects, hemolymph lipid levels were similar to those of controls up to 96 h post-treatment, then declined to lower than control levels; fat-body lipid content was initially higher, then lower, and finally higher, than that of controls. Fenoxycarb and methoprene caused severe, yet dissimilar, disruptions in metabolism of all three nutrients.

EFFECTS OF SELECTED JUVENILE HORMONE ANALOGS ON SIXTH-INSTAR LARVAE OF THE EASTERN SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA CLEMENS (LEPIDOPTERA: TORTRICIDAE)

1989 ◽  
Vol 121 (12) ◽  
pp. 1111-1116 ◽  
Author(s):  
Hemendra Mulye ◽  
Roger Gordon
Keyword(s):  
Juvenile Hormone ◽  
Choristoneura Fumiferana ◽  
Subsequent Development ◽  
Control Program ◽  
Spruce Budworm ◽  
Control Potential ◽  
Eastern Spruce Budworm ◽  
Hormone Analogs

AbstractThe effectiveness of seven insect juvenile hormone analogs (JHAs) in disrupting metamorphosis of the eastern spruce budworm, Choristoneura fumiferana Clemens, was determined by applying the compounds topically to early sixth-instar larvae and monitoring subsequent development. Fenoxycarb showed promising control potential, with an LC50 of 0.26 μg per insect. Relative lethal effectiveness, based on LC50 values, was Fenoxycarb > ZR9892 > ZR8487 > S-71639 > methoprene > ZR9582 > ZR10151. Some of the JHAs tested may be useful in an integrated spruce budworm control program.

Some cytological changes in the fat body and testes of male spruce budworm (Choristoneura fumiferana Clem.) pupae after treatment with tepa

1971 ◽  
Vol 49 (6) ◽  
pp. 831-836 ◽  
Author(s):  
Norton D. Addy
Keyword(s):  
Protein Metabolism ◽  
Protein Concentration ◽  
Fat Body ◽  
Choristoneura Fumiferana ◽  
Histological Study ◽  
Spruce Budworm ◽  
Protein Patterns ◽  
Dominant Lethal Mutation ◽  
Cytological Changes ◽  
Fat Bodies

A histological study was conducted to determine the effects of tepa, an aziridine-alkylating agent, on male spruce budworm pupae. The results indicated an increase in protein concentration in the fat body and testes of the tepa-treated insects. The profound changes in protein metabolism in the tepa-treated insects are similar to known protein patterns in fat bodies that are mediated by increases in ecdysone titer during the pupal period. These changes are further evident in the eye and testes pigment production and in the differentiating sperm that result in sterile gametes which all depend on ecdysone titer for their normal development. From this it seems evident that tepa may have an effect on the cell's ability to regulate hormone levels, protein metabolism, or both. And these effects result in the critical changes in mature sperm that lead to the dominant lethal mutation occurring in resulting zygotes.

EFFECTS OF THE JUVENILE HORMONE ANALOG FENOXYCARB ON VARIOUS DEVELOPMENTAL STAGES OF THE EASTERN SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (CLEMENS) (LEPIDOPTERA: TORTRICIDAE)

1992 ◽  
Vol 124 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Barry J. Hicks ◽  
Roger Gordon
Keyword(s):  
Juvenile Hormone ◽  
Developmental Stages ◽  
Early Stage ◽  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Larval Stages ◽  
Eastern Spruce Budworm ◽  
Adult Females ◽  
Juvenile Hormone Analog ◽  
Hormone Analog

AbstractThe effectiveness of topical applications of the juvenile hormone analog Fenoxycarb against selected developmental stages of the eastern spruce budworm, Choristoneura fumiferana (Clemens), was determined. Eggs at an early stage of embryogenesis (0–24 h old) were prevented from hatching and were more sensitive to the compound than older eggs (48–72 h old) and larval stages. Fifth-instar larvae displayed lethal morphogenetic effects following Fenoxycarb treatment, but third-instar larvae were refractory. Adult females constituted the most sensitive stage; treated insects laid eggs that failed to hatch. Untreated adult females that mated with Fenoxycarb-treated males also laid infertile eggs.

‘I saw a knock-out’: Fatness, (In)visibility, and Desire in Shallow Hal

Somatechnics ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 60-79 ◽  
Author(s):  
Jackie Wykes
Keyword(s):  
Female Body ◽  
Fat Body ◽  
Western Culture ◽  
Embodied Experience ◽  
Knock Out ◽  
Dominant Meaning ◽  
Starting Point ◽  
Female Bodies ◽  
Fat Bodies ◽  
Sexual Body

When the Farrelly brothers' movie Shallow Hal (2001) was released, one reviewer suggested that the film ‘might have been more honest if [it] had simply made Hal have a thing about fat women’ ( Kerr 2002 : 44). In this paper, I argue that Kerr hits the mark but misses the point. While the film's treatment of fat is undoubtedly problematic, I propose a ‘queer’ reading of the film, borrowing the idea of ‘double coding’ to show a text about desire for fat (female) bodies. I am not, however, seeking to position Shallow Hal as a fat-positive text; rather, I use it as a starting point to explore the legibility of the fat female body as a sexual body. In contemporary mainstream Western culture, fat is regarded as the antithesis of desire. This meaning is so deeply ingrained that representations of fat women as sexual are typically framed as a joke because desire for fat bodies is unimaginable; this is the logic by which Shallow Hal operates. The dominant meaning of fatness precludes recognition of the fat body as a sexual body. What is at issue is therefore not simply the lack of certain images, but a question of intelligibility: if the meaning of fat is antithetical to desire, how can the desire for – and of – fat bodies be intelligible as desire? This question goes beyond the realm of representation and into the embodied experience of fat sexuality.

Glycogen Phosphorylase in the Fat Body of Two Cockroach Species, Periplaneta americana and Nauphoeta cinerea: Isolation, Partial Characterization of Three Forms and Activation by Hypertrehalosaemic Hormones

1991 ◽  
Vol 46 (1-2) ◽  
pp. 149-162 ◽  
Author(s):  
Gerd Gäde
Keyword(s):  
Anion Exchange ◽  
Glycogen Phosphorylase ◽  
Periplaneta Americana ◽  
Fat Body ◽  
Phosphorylase Kinase ◽  
Phosphorylase Activity ◽  
Strong Inhibitor ◽  
Nauphoeta Cinerea ◽  
Crude Extracts ◽  
Fat Bodies

The presence of endogenous phosphorylase kinase and phosphorylase phosphatase in crude extracts of fat bodies from the cockroaches Nauphoeta cinerea and Periplaneta americana is demonstrated in vitro by activation/inactivation of glycogen phosphorylase under appropriate conditions. Fractionation of fat body extracts of both cockroach species on an anion-exchange medium results in the elution of three peaks with phosphorylase activity. According to their AMP dependency these activity peaks are designated as phosphorylase b (inactive without AMP), phosphorylase ab (active without AMP, but several stimulated with AMP) and phosphorylase a (active without AMP). It is shown chromatographically that incubating crude extracts of fat bodies from both cockroaches, under conditions where the phosphorylase kinase is active, results in all phosphorylase b being converted to the ab- or a-form , whereas under conditions where the phosphorylase phosphatase is active all phophorylase a is converted to the ab- or b-form . Endogenous phosphorylase kinase of N. cinerea crude fat body extract can convert vertebrate phosphorylase b into the a-form , and, conversely, vertebrate muscle p hosphorylase kinase and phosphorylase phosphatase, respectively, are able to convert partially purified N. cinerea phosphorylase aborb and the ab- und a-form , respectively. In resting cockroaches most of the phosphorylase activity resides in the b-form and only a small fraction (10% ) in the a-form , whereas between 26% (N . cinerea) and 35% (P. americana) occurs in the ab-form . Injection of endogenous hypertrehalosaemic peptides into N. cinerea (the decapeptide Bld-HrTH ) or P. americana (the two octapeptides Pea-CAH -I and II) causes interconversion of phosphorylase; after injection, mainly (60% ) phosphorylase a is present, while 25% and 15% exists in the ab- und b-form , respectively. Purification of the three phosphorylase forms from N. cinerea is achieved by anion-exchange chromatography on DEAE-Sephacel followed by affinity chromatography on AMP-Sepharose. The final specific activities are 2.1, 6.9 and 27.2 U /mg protein for the a-, ab- und b-form . The molecular mass of the active molecules on gel filtration is between 173,000 and 177,000, and SDS gel electrophoresis reveals a subunit mass of 87,100, suggesting a homodimeric structure for all three form s. Kinetic studies show hyperbolic saturation curves for the substrates glycogen and Pi respectively, with Kᴍ-values of 0.021, 0.019 and 0.073% for glycogen and 8.3, 6.3 and 17.9 mᴍ for Pi (a-, ab- and b-form ). Phosphorylase a exhibits a more or less hyperbolic response to AMP and needs 70 |iM A M P for m axim al stim ulation. The kinetics for the ab- and b-form s are sigm oidal and maximal activities are displayed at about 3 mᴍ (half-maximum activation as calculated from Hill plots are 55 and 280 μᴍ for the ab- und b-form , respectively). Caffeine is a strong inhibitor of the b-form , but has only a slight inhibiting effect (10 -20 % ) on the ab- and a-form in the presence of AMP.

scholarly journals Preliminary Evidence for a Relationship between Elevated Plasma TNFα and Smaller Subcortical White Matter Volume in HCV Infection Irrespective of HIV or AUD Comorbidity

2021 ◽  
Vol 22 (9) ◽  
pp. 4953
Author(s):  
Natalie M. Zahr ◽  
Kilian M. Pohl ◽  
Allison J. Kwong ◽  
Edith V. Sullivan ◽  
Adolf Pfefferbaum
Keyword(s):  
White Matter ◽  
Proinflammatory Cytokines ◽  
Soluble Protein ◽  
Subcortical White Matter ◽  
Control Group ◽  
White Matter Volume ◽  
Brain Volumes ◽  
Protein Levels ◽  
Matter Volume ◽  
Patient Groups

Classical inflammation in response to bacterial, parasitic, or viral infections such as HIV includes local recruitment of neutrophils and macrophages and the production of proinflammatory cytokines and chemokines. Proposed biomarkers of organ integrity in Alcohol Use Disorders (AUD) include elevations in peripheral plasma levels of proinflammatory proteins. In testing this proposal, previous work included a group of human immunodeficiency virus (HIV)-infected individuals as positive controls and identified elevations in the soluble proteins TNFα and IP10; these cytokines were only elevated in AUD individuals seropositive for hepatitis C infection (HCV). The current observational, cross-sectional study evaluated whether higher levels of these proinflammatory cytokines would be associated with compromised brain integrity. Soluble protein levels were quantified in 86 healthy controls, 132 individuals with AUD, 54 individuals seropositive for HIV, and 49 individuals with AUD and HIV. Among the patient groups, HCV was present in 24 of the individuals with AUD, 13 individuals with HIV, and 20 of the individuals in the comorbid AUD and HIV group. Soluble protein levels were correlated to regional brain volumes as quantified with structural magnetic resonance imaging (MRI). In addition to higher levels of TNFα and IP10 in the 2 HIV groups and the HCV-seropositive AUD group, this study identified lower levels of IL1β in the 3 patient groups relative to the control group. Only TNFα, however, showed a relationship with brain integrity: in HCV or HIV infection, higher peripheral levels of TNFα correlated with smaller subcortical white matter volume. These preliminary results highlight the privileged status of TNFα on brain integrity in the context of infection.

scholarly journals Mobilizing the Sense of “Fat”: A Phenomenological Materialist Approach

Human Studies ◽  
2021 ◽  
Author(s):  
Jenny Slatman
Keyword(s):  
Lived Experiences ◽  
Fat Body ◽  
The Body ◽  
New Materialism ◽  
Writing Style ◽  
Conceptual Frame ◽  
Fat Bodies ◽  
The Way ◽  
Phenomenological Exploration

AbstractThis paper aims to mobilize the way we think and write about fat bodies while drawing on Jean-Luc Nancy’s philosophy of the body. I introduce Nancy’s approach to the body as an addition to contemporary new materialism. His philosophy, so I argue, offers a form of materialism that allows for a phenomenological exploration of the body. As such, it can help us to understand the lived experiences of fat embodiment. Additionally, Nancy’s idea of the body in terms of a “corpus”—a collection of pieces without a unity—together with his idea of corpus-writing—fragmentary writing, without head and tail—can help us to mobilize fixed meanings of fat. To apply Nancy’s conceptual frame to a concrete manifestation of fat embodiment, I provide a reading of Roxane Gay’s memoir Hunger (2017). In my analysis, I identify how the materiality of fat engenders the meaning of embodiment, and how it shapes how a fat body can and cannot be a body. Moreover, I propose that Gay’s writing style—hesitating and circling – involves an example of corpus-writing. The corpus of corpulence that Gay has created gives voice to the precariousness of a fat body's materialization.

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