Selective utilization of fatty acids in rainbow trout (Oncorhynchus mykiss Walbaum) red muscle mitochondria

1993 ◽  
Vol 71 (2) ◽  
pp. 248-251 ◽  
Author(s):  
K.-H. Kiessling ◽  
A. Kiessling
Keyword(s):  
Fatty Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Oxidation Rate ◽  
Essential Fatty Acids ◽  
Muscle Mitochondria ◽  
Fat Depots ◽  
Oxidation Rates ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Red Muscle

This study was undertaken to establish whether fatty acids are generally oxidized at the same rate or if a pattern of oxidation rates exists to protect certain fatty acids from degradation for ATP formation. Beta-oxidation was studied in red muscle mitochondria of 10 major fatty acids that are acquired in the diet and occur in the fat depots of rainbow trout (Oncorhynchus mykiss Walbaum). The mitochondria were isolated by fractional centrifugation and the fatty acids were added as coenzyme A esters in the presence of carnitine. The fatty acids could be separated into roughly three groups in relation to their oxidation rates. Two fatty acids (14:0 and 16:0) were oxidized as rapidly as pyruvate. Another six acids (16:1 n − 7, 18:0, 18:1 n − 9, 20:1 n − 9, 22:1 n − 9 and 22:6 n − 3) were oxidized at about three-quarters to one-half the rate of pyruvate. The two essential fatty acids (18:2 n − 6 and 18:3 n − 3) had a slow oxidation rate, about one-fifth of that of pyruvate. The liver mitochondria from rainbow trout oxidized 18:0, 18:1, 18:2, and 18:3 at the same rate, 70–80% of that of pyruvate. These results show that rainbow trout red muscle discriminates between fatty acids used in energy production and essential fatty acid precursors, as indicated by the low β-oxidation rate of the latter acids.

Evaluation of the composition of essential fatty acids in the tissues of rainbow trout (Oncorhynchus mykiss) grown in closed water supply units

2021 ◽  
Vol 325 (4) ◽  
pp. 495-501
Author(s):  
E.A. Zykina ◽  
M.V. Gurin
Keyword(s):  
Fatty Acids ◽  
Rainbow Trout ◽  
Water Supply ◽  
Oncorhynchus Mykiss ◽  
Fish Oil ◽  
Essential Fatty Acids ◽  
The Body ◽  
Biologically Active ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Aquaculture Products

Recently, the task of artificial cultivation of especially valuable fish species in aquaculture has become more and more urgent. The value of salmon fish, and in particular rainbow trout Oncorhynchus mykiss (Walbaum, 1792) available for breeding, is largely determined by the high content of biologically active essential and polyunsaturated fatty acids of the group ω-6 and ω-3, necessary for vital activity and not synthesized by the human body. Since the natural stocks of many popular fatty fish have decreased, farmed fish can help meet consumer demand for this product. Based on this, the assessment of the content of the main ω-6 and ω-3 fatty acids in the fat of rainbow trout grown in closed-loop water supply installations (USV) was made. The fat was extracted from the fish by thermal method with further distillation by supercritical fluid extraction on the SFT-150 unit. The ratio of fatty acids in the fish oil sample was determined using the Chromatek-Kristall-5000 M hardware and software complex. The results obtained were compared with the standard for salmon fish oil “Aquaculture products” and “Wild fish”. It is established that trout cultivated in the UZV in the Penza region, in terms of the content of the main essential fatty acids, is not inferior to the quality indicators of the standard in terms of the requirements for salmon fish of “Aquaculture products”. In fish, all essential fatty acids are present in sufficient quantities, the ratio of ω-6 to ω-3 is 2.6:1, which indicates a high nutritional value of the product and allows it to be used in human food to provide the body with essential fatty acids, as well as a raw material for obtaining therapeutic and preventive products.

scholarly journals Apparent Digestibility of Macronutrients and Fatty Acids from Microalgae (Schizochytrium sp.) Fed to Rainbow Trout (Oncorhynchus mykiss): A Potential Candidate for Fish Oil Substitution

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 456
Author(s):  
Amélie Bélanger ◽  
Pallab K. Sarker ◽  
Dominique P. Bureau ◽  
Yvan Chouinard ◽  
Grant W. Vandenberg
Keyword(s):  
Fatty Acids ◽  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Fish Oil ◽  
Nutrient Digestibility ◽  
Fish Feed ◽  
Potential Candidate ◽  
Apparent Digestibility ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Aquaculture Feed

Aquaculture feed formulation has recently turned its focus to reduce the reliance on marine-derived resources and utilise alternative feedstuffs, as an approach to improve the environmental sustainability of the aquaculture sector. The fish oil market is highly volatile, and availability of this commodity is continuously decreasing for use in aquaculture. Currently, a growing number of commercial efforts producing microalgae are providing omega 3-rich oil for sustainable aquaculture feed. This study was focused to determine the nutrient digestibility of a marine microalga, Schizochytrium spp., which is rich in docosahexaenoic acid (DHA) and long-chain polyunsaturated fatty acids (LC-PUFA), as a novel dietary lipid source that could be utilized effectively by rainbow trout (Oncorhynchus mykiss). A whole-cell Schizochytrium spp. biomass was used in the digestibility experiment at two different temperatures, 8 °C and 15 °C. No significant differences were detected between the two temperatures for the apparent digestibility coefficients (ADCs) of the dry matter (94.3 ± 4.9%), total lipids (85.8 ± 0.0%), crude proteins (89.5 ± 1.8%), energy (83.1 ± 1.7%) and fatty acids (85.8 ± 7.5%). The ADCs of the nutrients, energy, DHA and other fatty acids showed that Schizochytrium spp. is a high-quality candidate for fish oil substitution and supplement of LC-PUFA in fish feed with vegetable oils.

scholarly journals Qualitative properties of rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from aquaculture facility in Bitola region (Macedonia)

2017 ◽  
Vol 33 (1) ◽  
pp. 91-102
Author(s):  
Dijana Blazhekovikj-Dimovska ◽  
Biljana Sivakova
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Rainbow Trout ◽  
Chemical Composition ◽  
Oncorhynchus Mykiss ◽  
Acid Composition ◽  
Microbiological Analysis ◽  
Qualitative Properties ◽  
Rainbow Trout Oncorhynchus Mykiss

The main goal of this research was to determine the qualitative properties of the rainbow trout (Oncorhynchus mykiss Walbaum, 1792) from aquaculture facility - salmonid fishpond Strezhevo which is situated in Bitola region (Republic of Macedonia). The qualitative properties of the rainbow trout are established by determination of the chemical and the fatty acid composition of the fish meat, the energy value of the meat and the microbiological analysis for the total number of microorganisms on fish skin and presence of Salmonella sp. and Listeria monocytogenes. The main purpose of the research produced additional analyzes that determine the physical - chemical properties and also a microbiological analysis of the water in which the rainbow trout resides, the chemical composition of feed used for feeding of the rainbow trout, the condition factor (CF) and the feed conversion. The results obtained during the examination of the chemical composition of the rainbow trout meat from the fishpond Strezhevo determined the mean value of 74.533% water, 20.600% protein, 3.366% fat and 1.38% ash. The energy value of the meat was 484.635 kJ/100 g. Considering the results of the fatty acid composition of the rainbow trout from the fishpond Strezhevo, it can be concluded that the content of the saturated fatty acids (SFA) is 20.303%, the monounsaturated fatty acids (MUFA) is 52.359% and the polyunsaturated fatty acids (PUFA) is 27.268%. In terms of the amount of n-6 fatty acids, it is 20.180%, while the amount of n-3 is 7.088%.

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