A fluorine-19 nuclear magnetic resonance study of 5-fluorouracil metabolism in Mesocestoides corti tetrathyridia

1992 ◽  
Vol 70 (2) ◽  
pp. 406-410
Author(s):  
Marie M. A. Novak ◽  
Katherine Shimizu ◽  
Barry J. Blackburn
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
Nuclear Magnetic Resonance Spectra ◽  
Composite Peak ◽  
Magnetic Resonance Spectra ◽  
Nuclear Magnetic

Fluorine-19 nuclear magnetic resonance spectra of live tetrathyridia of Mesocestoides corti incubated in 10 mM 5-fluorouracil (5-FU) showed that these larvae metabolize 5-FU to α-fluoro-β-ureidopropionic acid (FUPA), α-fluoro-β-alanine (FBA1) and fluoronucleotides (FNuct). This metabolism is predominantly catabolic, since as time progressed (up to 7 h) the 5-FU signal continuously decreased while the FBA1 signal correspondingly increased and the composite FNuct peak remained small. Similar spectra were obtained from acid extracts of tetrathyridia, except that an additional, very small fluoronucleoside (FNuc) composite peak could be detected. We found 5-FU to be unsuccessful in inhibiting the growth of M. corti tetrathyridia in mice; it is postulated that this was due to the failure of the larvae to effectively anabolize 5-FU.

A 14N nuclear magnetic resonance study on nitrogen–oxygen–halogen compounds

1969 ◽  
Vol 47 (22) ◽  
pp. 4247-4251 ◽  
Author(s):  
A. M. Qureshi ◽  
J. A. Ripmeester ◽  
F. Aubke
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
Side Band ◽  
Nuclear Magnetic Resonance Spectrometer ◽  
Halogen Compounds ◽  
Nuclear Magnetic Resonance Spectra ◽  
Line Nuclear Magnetic Resonance ◽  
Nuclear Magnetic

14N nuclear magnetic resonance spectra of nitrogen oxides, fluorides, and oxyhalides have been recorded using direct measurement with a broad line nuclear magnetic resonance spectrometer. Also investigated are some nitrogen-containing fluoro- and oxo-cations in protonic solvents. Using the side band technique, fine structure due to 14N–19F spin–pin interaction could be resolved for nitrogen trifluoride, nitrogenoxide trifluoride, and nitrylfluoride.

A nuclear magnetic resonance study of the conformations of triribonucleoside diphosphates, diribonucleoside phosphates, 5′-ribonucleoside phosphates, and ribonucleosides of uracil and cytosine; temperature dependence of the base proton chemical shifts

1969 ◽  
Vol 47 (3) ◽  
pp. 513-515 ◽  
Author(s):  
Ian C. P. Smith ◽  
B. J. Blackburn ◽  
Tetsuo Yamane
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Temperature Dependence ◽  
Chemical Shifts ◽  
Nuclear Magnetic Resonance Study ◽  
Magnetic Resonance Study ◽  
Nuclear Magnetic Resonance Spectra ◽  
Anti Conformation ◽  
Proton Chemical Shifts ◽  
Nuclear Magnetic

The temperature dependence of the nuclear magnetic resonance spectra of U, C, 5′-UMP, 5′-CMP, UpU, and CpU indicates a specific deshielding of the base proton at position-6 by an hydroxyl, phosphodiester, or phosphate group at position-5′, and demonstrates that in these compounds the bases exist in the anti conformation. Similar experiments on the wobble pair UpUpU and UpUpC suggest that the spatial arrangements of both trinucleoside diphosphates are identical, and that in these arrangements the bases are predominantly in the anti conformation.

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