Morphology of the hemipenes of some Amphisbaenia (Reptilia: Squamata)

1991 ◽  
Vol 69 (2) ◽  
pp. 359-368 ◽  
Author(s):  
Herbert I. Rosenberg ◽  
Michael J. Cavey ◽  
Carl Gans
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Outer Surface ◽  
Scanning Electron

Preserved amphisbaenids with fully everted hemipenes are rare and few studies have described these intromittent organs in detail. Some previous descriptions are misleading as they are based on partially everted hemipenes. In this paper the hemipenes of several species of amphisbaenids are described on the basis of gross anatomical, histological, and scanning electron microscopy observations. Study of a series of specimens of Amphisbaena caeca and of A. innocens indicates that many hemipenes were not fully everted during fixation. The typical amphisbaenian hemipenis consists of a main truncus that bifurcates to form two lobes. The distal tips of the lobes may bear fields of lamellae that surround the termination of the sulcus spermaticus. Various regions of the outer surface of the lobes may also bear microornamentation. Some possible roles of lamellae and papillae during copulation are discussed.

scholarly journals Characteristics of the surface of the epidermis in floral nectaries and the receptacle of mountain ash (Sorbus aucuparia L.)

2012 ◽  
Vol 60 (2) ◽  
pp. 23-30
Author(s):  
Agata Konarska
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Outer Surface ◽  
Flower Development ◽  
Epidermal Cells ◽  
Sorbus Aucuparia ◽  
Mountain Ash ◽  
Floral Nectaries ◽  
Scanning Electron

The structure of receptacular surfaces of floral nectaries at two flowering stages and the structure of the outer surface of the receptacle of <i>Sorbus aucuparia</i> were investigated using scanning electron microscopy. Changes in the development of the cuticular epithelium of the nectary epidermis and differences in the degree of aperture of stomata were observed. Increased undulation of the gland surface was found during flower development. Numerous stomata were situated slightly below the level of epidermal cells of the nectary. At the pollination stage, open pores or pores surrounded by the cuticular epithelium were observed, as well as covered by dried secretion. Dried nectar in the form of patches was also visible on the surface of the gland. Stomata of the outer surface of the receptacle were located on protrusions and surrounded by the cuticular epithelium.

scholarly journals Enterohemorrhagic Escherichia coli O157:H7 Present in Radish Sprouts

1998 ◽  
Vol 64 (4) ◽  
pp. 1532-1535 ◽  
Author(s):  
Yoshinori Itoh ◽  
Yoshiko Sugita-Konishi ◽  
Fumiko Kasuga ◽  
Masaaki Iwaki ◽  
Yukiko Hara-Kudo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Scanning Electron Microscopy ◽  
Outer Surface ◽  
Immunofluorescence Microscopy ◽  
Enterohemorrhagic Escherichia Coli ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Radish Sprouts ◽  
Scanning Electron

ABSTRACT Using cultivation, immunofluorescence microscopy, and scanning electron microscopy, we demonstrated the presence of viable enterohemorrhagic Escherichia coli O157:H7 not only on the outer surfaces but also in the inner tissues and stomata of cotyledons of radish sprouts grown from seeds experimentally contaminated with the bacterium. HgCl2 treatment of the outer surface of the hypocotyl did not kill the contaminating bacteria, which emphasized the importance of either using seeds free from E. coli O157:H7 in the production of radish sprouts or heating the sprouts before they are eaten.

scholarly journals Morphology of Metzgeria conjugata Lindb. (Metzgeriales, Hepaticopsida)

1992 ◽  
Vol 6 (1) ◽  
pp. 65-69
Author(s):  
Denise Pinheiro Da Costa ◽  
Raul Dodsworth Machado
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Outer Surface ◽  
Dorsal Surface ◽  
Ventral Surface ◽  
Epidermal Cells ◽  
Scanning Electron

Scanning electron microscopy and light microscopy were used to elucidate the morphology of Metzgeria conjutata Lindb. and confirm the presence of 2 rows of epidermal cells on the dorsal surface, (21-3) rows on the ventral surface, midrib with cells in (3-51-6) tiers; hirsute, short hairs, straight on the thallus-margin and on the ventral surface of midrib; marginal hairs paired, single or in groups of three; male branches globose or subglobose; female involucres obovate and hirsute at the margin, calyptra fleshy, pyriform to club-shaped, hirsute on the outer surface, hairs long and straight.

Developing protoscoleces ofEchinococcus granulosuson the outer surface of the brood capsule, detected by scanning electron microscopy

1976 ◽  
Vol 50 (2) ◽  
pp. 75-77 ◽  
Author(s):  
R. C. A. Thompson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Echinococcus Granulosus ◽  
Outer Surface ◽  
Histological Studies ◽  
Complete Development ◽  
External Development ◽  
Scanning Electron

AbstractBy means of scanning electron microscopy, stalked protrusions were observed arising from the outer surface of intact brood capsules ofEchinococcus granulosus. Histological studies showed these protrusions to be developing protoscoleces. However, complete development is not attained and the protoscoleces eventually die. It is suggested that external development is a result of overcrowding within the brood capsule.

Stigma and Style Morphology in Relation to Taxonomy and Breeding Systems in Eucalyptus and Angophora (Myrtaceae)

1986 ◽  
Vol 34 (5) ◽  
pp. 569 ◽  
Author(s):  
DJ Boland ◽  
M Sedgley
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Outer Surface ◽  
Breeding Systems ◽  
Cohesive Group ◽  
Varying Length ◽  
Scanning Electron

The stigma and style of 94 species of Eucalyptus and two species of Angophora were studied by scanning electron microscopy and/or light microscopy. All species had papillate stigmas and a stylar canal of varying length. Angophora species had mop-like stigmas with long papillae that were very similar in appearance to those of the red bloodwood group of the Corymbia, e.g. E. gummifera. The spotted gum group of the Corymbia had mop-like stigmas with short papillae and the yellow bloodwoods had tapered stigmas. The latter group was also charaderised by an extremely thick cuticle on the outer surface of the style, over 100 �m in thickness in E. watsoniana. All species in Blakella had tapered stigmas with a lobed surface and relatively few short papillae. The stylar canal had no cuticle in E. papuana. Eudesmia is a variable subgenus with E. erythrocorys unusual in having long multicellular papillae. Most Symphyomyrtus species had blunt or pinhead-shaped lobed stigmas with a heavily cutinised stylar canal. E. deglupta and E. microcorys did not conform to this pattern and had mop-shaped stigmas with long papillae. Monocalyptus species had blunt stigmas with few papillae and hollow styles and appeared to form a cohesive group. On the basis of stigma and style morphology Angophora is more similar to Corymbia than to Blakella. E. deglupta and E. microcorys are distinct from other Symphyomyrtus species studied. E. trachyphloia and E. jacobsiana are more similar to E. gummifera than to E. watsoniana or other yellow bloodwoods.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Spontaneous Cataract in Nude Athymic Mice

1977 ◽  
Vol 35 ◽  
pp. 512-513
Author(s):  
Ronald H. Bradley ◽  
R. S. Berk ◽  
L. D. Hazlett
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Nude Mouse ◽  
Cellular Immunity ◽  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Athymic Mice ◽  
Transmission Microscopy ◽  
Mouse Lens ◽  
Scanning Electron

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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