The photoreceptors and visual pigments in the retina of the white sturgeon, Acipenser transmontanus

1990 ◽  
Vol 68 (7) ◽  
pp. 1544-1551 ◽  
Author(s):  
A. J. Sillman ◽  
M. D. Spanfelner ◽  
E. R. Loew
Keyword(s):  
Vitamin A ◽  
Visual Pigment ◽  
Visual Pigments ◽  
Spectrophotometric Analysis ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Light Regimen ◽  
Cone Pigment ◽  
Spectral Absorbance

The photoreceptors in the retina of the white sturgeon, Acipenser transmontanus (Chondrostei), were studied by means of scanning electron microscopy, in situ microspectrophotometry, and spectrophotometric analysis of visual pigment extracts. The white sturgeon retina is simple in that it contains only two morphologically distinct photoreceptors. The retina is dominated by rods with large outer segments, but there is a substantial population (40%) of single cones. Evidence was found for only one rod visual pigment and one cone visual pigment. Peak spectral absorbance (λmax) of the rod pigment is near 539 nm, whereas λmax of the cone pigment is near 605 nm. Both visual pigments are porphyropsin types with chromophores based on vitamin A2. No detectable rhodopsin based on vitamin A1 is ever present, regardless of season or light regimen. The results are discussed in terms of the sturgeon's behavior, as well as the implications for the evolution of color vision.

The visual pigments of wild white sturgeon (Acipenser transmontanus)

1995 ◽  
Vol 73 (4) ◽  
pp. 805-809 ◽  
Author(s):  
A. J. Sillman ◽  
M. E. Sorsky ◽  
E. R. Loew
Keyword(s):  
Vitamin A ◽  
Fresh Water ◽  
Visual Pigment ◽  
Visual Pigments ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Estuarine Water ◽  
Cone Pigments ◽  
Partial Bleaching

The visual pigments of the anadromous white sturgeon (Acipenser transmontanus) taken from relatively saline estuarine water were characterized by means of in situ microspectrophotometry and partial bleaching analysis of a digitonin extract. The three cone pigments (λmax = 605, 539, and ca. 460 nm) and one rod pigment (λmax = 541 nm) of the wild sturgeon are the same as those of cultured sturgeon that spend their entire lives in fresh water. All the visual pigments incorporate a chromophore based on vitamin A2. Unlike other anadromous fishes during the "saline phase," the white sturgeon shows no evidence of the presence of any vitamin A1 based visual pigment in the retina.

Age-related changes in the visual pigments of the white sturgeon (Acipenser transmontanus)

1993 ◽  
Vol 71 (8) ◽  
pp. 1552-1557 ◽  
Author(s):  
E. R. Loew ◽  
A. J. Sillman
Keyword(s):  
Visual Pigment ◽  
Visual Pigments ◽  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Disulfonic Acid ◽  
Fluorescent Substance ◽  
Rods And Cones ◽  
Age Related ◽  
Juvenile Sturgeon ◽  
Spectral Absorbance

Using in situ microspectrophotometry, the spectral absorbance characteristics of the photoreceptors in the retinas of larval, juvenile, and adult white sturgeon (Acipenser transmontanus) were determined. The adult has one type of rod, containing a visual pigment with maximum spectral absorbance (λmax) near 540 nm. There are three types of cones, morphologically identical but distinguished from one another by containing either a blue-sensitive (λmax 464 nm), green-sensitive (λmax 531 nm), or red-sensitive (λmax 605 nm) visual pigment. Juvenile sturgeon have visual pigments similar to those of the adult. However, no evidence could be found for the presence of either blue-sensitive or red-sensitive cones in larval white sturgeon through the age of 10 weeks. Larval sturgeon up to about 10 weeks yielded only green-sensitive rods and cones. The absence of red-sensitive cones in the larvae, and their presence in older fish, was confirmed by the use of 4,4′-diisothiocyanato-stilbene-2,2′-disulfonic acid, a fluorescent substance that binds selectively to photoreceptors sensitive to long-wavelength light. Regardless of age, all visual pigments are based on vitamin A2. Also regardless of age, white sturgeon retinas yielded no evidence for the presence of photoreceptors sensitive to ultraviolet light.

Spectral absorbance, structure, and population density of photoreceptors in the retina of the lake sturgeon (Acipenser fulvescens)

2007 ◽  
Vol 85 (4) ◽  
pp. 584-587 ◽  
Author(s):  
A.J. Sillman ◽  
E.K. Ong ◽  
E.R. Loew
Keyword(s):  
Visual Pigment ◽  
Visual Pigments ◽  
Lake Sturgeon ◽  
Acipenser Fulvescens ◽  
Long Wavelength ◽  
Rods And Cones ◽  
The North ◽  
Spectral Absorbance ◽  
Dim Light ◽  
Scanning Electron

Lake sturgeon ( Acipenser fulvescens Rafinesque, 1817) photoreceptors were studied with scanning electron microscopy and microspectrophotometry. The retina contains both rods and cones, with cones estimated composing about 30% of the photoreceptor population. Only large single cones were identified and they are similar to those found in other species of the order Acipenseriformes. The rods are large, with long, broad outer segments, and are similar to the dominant rod found in other sturgeons and the North American paddlefish ( Polyodon spathula (Walbaum, 1792)). Mean (SD) rod packing density at 22 624 ± 3 509 rods/mm2 is low compared with those of other animals that function primarily in dim light. The visual pigment of the rods has a mean (SD) peak absorbance (λmax) at 541 ± 2 nm. Three different cone populations were identified: a long wavelength sensitive cone containing a visual pigment with λmax at 619 ± 3 nm; middle wavelength sensitive cone with λmax at 538 ± 1 nm; and short wavelength sensitive cone with λmax at 448 ± 1 nm. All the visual pigments are based on the vitamin A2 chromophore.

scholarly journals In search of the visual pigment template

2000 ◽  
Vol 17 (4) ◽  
pp. 509-528 ◽  
Author(s):  
VICTOR I. GOVARDOVSKII ◽  
NANNA FYHRQUIST ◽  
TOM REUTER ◽  
DMITRY G. KUZMIN ◽  
KRISTIAN DONNER
Keyword(s):  
Visual Pigment ◽  
Good Description ◽  
Visual Pigments ◽  
Rod Outer Segments ◽  
Absorbance Spectrum ◽  
Outer Segments ◽  
Bovine Rhodopsin ◽  
Α Band ◽  
Absorbance Spectra

Absorbance spectra were recorded by microspectrophotometry from 39 different rod and cone types representing amphibians, reptiles, and fishes, with A1- or A2-based visual pigments and λmax ranging from 357 to 620 nm. The purpose was to investigate accuracy limits of putative universal templates for visual pigment absorbance spectra, and if possible to amend the templates to overcome the limitations. It was found that (1) the absorbance spectrum of frog rhodopsin extract very precisely parallels that of rod outer segments from the same individual, with only a slight hypsochromic shift in λmax, hence templates based on extracts are valid for absorbance in situ; (2) a template based on the bovine rhodopsin extract data of Partridge and De Grip (1991) describes the absorbance of amphibian rod outer segments excellently, contrary to recent electrophysiological results; (3) the λmax/λ invariance of spectral shape fails for A1 pigments with small λmax and for A2 pigments with large λmax, but the deviations are systematic and can be readily incorporated into, for example, the Lamb (1995) template. We thus propose modified templates for the main “α-band” of A1 and A2 pigments and show that these describe both absorbance and spectral sensitivities of photoreceptors over the whole range of λmax. Subtraction of the α-band from the full absorbance spectrum leaves a “β-band” described by a λmax-dependent Gaussian. We conclude that the idea of universal templates (one for A1- and one for A2-based visual pigments) remains valid and useful at the present level of accuracy of data on photoreceptor absorbance and sensitivity. The sum of our expressions for the α- and β-band gives a good description for visual pigment spectra with λmax > 350 nm.

scholarly journals Ultraviolet and violet receptors express identical mRNA encoding an ultraviolet-absorbing opsin: identification and histological localization of two mRNAs encoding short-wavelength-absorbing opsins in the retina of the butterfly Papilio xuthus

2000 ◽  
Vol 203 (19) ◽  
pp. 2887-2894 ◽  
Author(s):  
J. Kitamoto ◽  
K. Ozaki ◽  
K. Arikawa
Keyword(s):  
Amino Acid ◽  
Visual Pigment ◽  
Short Wavelength ◽  
Photoreceptor Cells ◽  
Visual Pigments ◽  
Amino Acid Sequences ◽  
Papilio Xuthus ◽  
Different Types ◽  
Identical Amino Acid Sequence

This paper describes the primary structures of two opsins of short-wavelength-absorbing visual pigments deduced from the mRNA sequences in the retina of the Japanese yellow swallowtail butterfly Papilio xuthus. A phylogenetic analysis of the amino acid sequences indicates that one of these visual pigments is of the ultraviolet-absorbing type and that the other is of the blue-absorbing type. We identified the photoreceptor cells that express these mRNAs by histological in situ hybridization. The mRNA of the ultraviolet type is expressed in two distinct photoreceptor types previously identified as ultraviolet and violet receptors, providing the first molecular biological evidence that different types of spectral receptor probably express a visual pigment with an identical amino acid sequence. The mRNA of the blue type is expressed exclusively in cells classified as blue receptors.

The Organ Specific Action of Thyroxin in Visual Pigment Differentiation

Development ◽  
1959 ◽  
Vol 7 (4) ◽  
pp. 556-563
Author(s):  
Fred H. Wilt
Keyword(s):  
Double Bond ◽  
Vitamin A ◽  
Visual Pigment ◽  
Rana Catesbeiana ◽  
Visual Pigments ◽  
Essential Difference ◽  
Phylogenetic Distribution ◽  
Chromophore Group ◽  
Vitamin A Metabolism ◽  
Organ Specific

In the course of his studies on the phylogenetic distribution of the retinal photopigments, Wald (1942, 1946, 1947) observed that the visual pigment of larvae of Rana catesbeiana changed from porphyropsin to rhodopsin during metamorphosis. The essential difference between the two visual pigments, which are conjugated proteins, is in the chromophore group, vitamin A aldehyde (or retinene). Vitamin A-2 aldehyde is the chromophore of porphyropsin; vitamin A-l aldehyde, which has one less double bond in its beta ionone ring, is the chromophore of rhodopsin (reviewed by Dartnall, 1958). The phenomenon of visual pigment conversion during metamorphosis has recently been examined in detail by Wilt (1959). His findings confirmed Wald's earlier report fully; furthermore, it was demonstrated that administration of thyroxin to premetamorphic animals stimulated photopigment conversion. Other evidence was presented supporting the hypothesis that thyroxin, or its physiologically active derivative, effects a change in vitamin A metabolism which results in a change in the type of chromophore on the visual protein.

scholarly journals Two visual pigments in a single photoreceptor cell: identification and histological localization of three mRNAs encoding visual pigment opsins in the retina of the butterfly Papilio xuthus.

1998 ◽  
Vol 201 (9) ◽  
pp. 1255-1261 ◽  
Author(s):  
J Kitamoto ◽  
K Sakamoto ◽  
K Ozaki ◽  
Y Mishina ◽  
K Arikawa
Keyword(s):  
In Situ Hybridization ◽  
Visual Pigment ◽  
Photoreceptor Cell ◽  
Wavelength Region ◽  
Photoreceptor Cells ◽  
Visual Pigments ◽  
Cell Identification ◽  
Papilio Xuthus

This paper describes the localization of newly identified visual pigment opsins in the tiered retina of the Japanese yellow swallowtail Papilio xuthus. We first cloned three cDNAs encoding visual pigment opsins, PxRh1, PxRh2 and PxRh3, and then carried out histological in situ hybridization to localize their mRNAs in the retina. By combining the present data with our previous electrophysiological results, we concluded that both PxRh1 and PxRh2 correspond to visual pigments expressed in photoreceptor cells sensitive in the green wavelength region (green receptors), whereas PxRh3 corresponds to a pigment in red receptors. The in situ hybridization studies showed that some photoreceptor cells express two opsin mRNAs. In the ventral half of the eye, all green receptors in the distal tier were labelled by both PxRh1 and PxRh2 probes. The labelling by the PxRh2 and PxRh3 probes was detected throughout the eye in the proximal tier; in 18 % of ommatidia, the probes labelled the same photoreceptor cell. These results suggest that the possible co-localization of two different visual pigments will broaden the sensitivity spectrum of the photoreceptor cells.

Co-infection of Acipenserid herpesvirus 2 (AciHV-2) and Streptococcus iniae in cultured white sturgeon Acipenser transmontanus

2017 ◽  
Vol 124 (1) ◽  
pp. 11-20 ◽  
Author(s):  
E Soto ◽  
C Richey ◽  
B Stevens ◽  
S Yun ◽  
K Kenelty ◽  
...  
Keyword(s):  
White Sturgeon ◽  
Acipenser Transmontanus ◽  
Streptococcus Iniae
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