The effect of diet on lipid deposition in Peromyscus maniculatus

1990 ◽  
Vol 68 (7) ◽  
pp. 1473-1475 ◽  
Author(s):  
Kimberley Mawhinney ◽  
John S. Millar
Keyword(s):  
Diet Quality ◽  
Diet Composition ◽  
Peromyscus Maniculatus ◽  
Fat Deposition ◽  
Lipid Deposition ◽  
Body Lipid ◽  
Total Body

Lipid deposition in Peromyscus maniculatus was examined to test the hypothesis that phenotypic fat deposition is a consequence of diet composition. Eighty P. maniculatus were divided into four groups of 20 and maintained on experimental diets varying in fat and protein. After 30 days, the mice were killed, necropsied and analyzed for lipid deposition. Changes in diet quality were not reflected in major changes in total body lipid. Changes in gut dimensions were sufficient to compensate for differences in diet and enabled the mice to maintain the same levels of lipid deposition on all diets.

scholarly journals Transcriptome analysis of miRNA and mRNA in the livers of pigs with highly diverged backfat thickness

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kai Xing ◽  
Xitong Zhao ◽  
Hong Ao ◽  
Shaokang Chen ◽  
Ting Yang ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Energy Metabolism ◽  
Differentially Expressed Genes ◽  
Regulatory Network ◽  
Acid Synthesis ◽  
Fat Deposition ◽  
Differentially Expressed ◽  
Backfat Thickness ◽  
Lipid Deposition ◽  
Differentially Expressed Mirnas

AbstractFat deposition is very important in pig production, and its mechanism is not clearly understood. MicroRNAs (miRNAs) play critical roles in fat deposition and energy metabolism. In the current study, we investigated the mRNA and miRNA transcriptome in the livers of Landrace pigs with extreme backfat thickness to explore miRNA-mRNA regulatory networks related to lipid deposition and metabolism. A comparative analysis of liver mRNA and miRNA transcriptomes from pigs (four pigs per group) with extreme backfat thickness was performed. We identified differentially expressed genes from RNA-seq data using a Cufflinks pipeline. Seventy-one differentially expressed genes (DEGs), including twenty-eight well annotated on the porcine reference genome genes, were found. The upregulation genes in pigs with higher backfat thickness were mainly involved in fatty acid synthesis, and included fatty acid synthase (FASN), glucokinase (GCK), phosphoglycerate dehydrogenase (PHGDH), and apolipoprotein A4 (APOA4). Cytochrome P450, family 2, subfamily J, polypeptide 34 (CYP2J34) was lower expressed in pigs with high backfat thickness, and is involved in the oxidation of arachidonic acid. Moreover, 13 differentially expressed miRNAs were identified. Seven miRNAs were associated with fatty acid synthesis, lipid metabolism, and adipogenic differentiation. Based on comprehensive analysis of the transcriptome of both mRNAs and miRNAs, an important regulatory network, in which six DEGs could be regulated by differentially expressed miRNAs, was established for fat deposition. The negative correlate in the regulatory network including, miR-545-5p and GRAMD3, miR-338 and FASN, and miR-127, miR-146b, miR-34c, miR-144 and THBS1 indicate that direct suppressive regulation may be involved in lipid deposition and energy metabolism. Based on liver mRNA and miRNA transcriptomes from pigs with extreme backfat thickness, we identified 28 differentially expressed genes and 13 differentially expressed miRNAs, and established an important miRNA-mRNA regulatory network. This study provides new insights into the molecular mechanisms that determine fat deposition in pigs.

Model of spontaneous obesity in aging male Wistar rats

1990 ◽  
Vol 259 (6) ◽  
pp. R1117-R1125 ◽  
Author(s):  
F. D. Newby ◽  
M. DiGirolamo ◽  
G. A. Cotsonis ◽  
M. H. Kutner
Keyword(s):  
Body Weight ◽  
Lipid Content ◽  
Wistar Rats ◽  
Lipid A ◽  
Curvilinear Relationship ◽  
Body Lipid ◽  
Fat Depots ◽  
Male Wistar Rats ◽  
Total Body ◽  
Relationship Of

We analyzed retrospectively data from 148 chow-fed male Wistar rats killed between the age of 6 wk and 2 yr while varying in body weight from 136 to 917 g. The purpose of this study was to clarify the relationship of body weight and body lipid content with the composition and cellularity of the epididymal and retroperitoneal fat depots. A positive linear association was found between body weight and body water or fat-free dry residue, whereas total body lipid exhibited a curvilinear relationship with body weight. The weight of the epididymal pads was linearly related to body weight but not to body lipid. In contrast, retroperitoneal pad weight was exponentially related to body weight and paralleled total body lipid. A strong linear correlation was found between total body lipid and weight (r = 0.959) or depot lipid content (r = 0.967) of the retroperitoneal fat pads. In this rat model of aging and spontaneous obesity, significant regional differences exist in adipose depot composition and cellularity. A practical outcome of this study is a simple and accurate prediction of body lipid content from the gravimetric determination of the retroperitoneal fat depots.

Neck and Total Body Fat Deposition in Nonobese and Obese Patients with Sleep Apnea Compared with That in Control Subjects

1998 ◽  
Vol 157 (1) ◽  
pp. 280-283 ◽  
Author(s):  
I. L. MORTIMORE ◽  
I. MARSHALL ◽  
P. K. WRAITH ◽  
R. J. SELLAR ◽  
N. J. DOUGLAS
Keyword(s):  
Sleep Apnea ◽  
Body Fat ◽  
Fat Deposition ◽  
Obese Patients ◽  
Control Subjects ◽  
Total Body Fat ◽  
Total Body

Prediction of total body lipid from total body water in rats. Part 1. Relations between directly measured major body components

Nahrung/Food ◽  
1991 ◽  
Vol 35 (6) ◽  
pp. 581-590 ◽  
Author(s):  
L. Zahn ◽  
R. Freund ◽  
K. Hoppe ◽  
K. L. Pisarchuk ◽  
W. Augustin
Keyword(s):  
Total Body Water ◽  
Body Water ◽  
Body Lipid ◽  
Body Components ◽  
Total Body

Differential changes in body composition during growth and progesterone treatment in intact female rats

1982 ◽  
Vol 93 (3) ◽  
pp. 391-395 ◽  
Author(s):  
J. P. Ashby ◽  
D. Shirling ◽  
J. D. Baird
Keyword(s):  
Body Composition ◽  
Body Fat ◽  
Fat Deposition ◽  
Protein Component ◽  
Female Rats ◽  
Intact Female ◽  
Progesterone Treatment ◽  
Total Body Fat ◽  
Accelerated Growth ◽  
Total Body

Female rats implanted with progesterone gained weight more rapidly than control animals and had a greater percentage of total body fat. The proportion of fat in the carcase of control animals also increased as they gained weight. Comparison of progesterone-treated rats with a group of weight-matched controls demonstrated that the effects of progesterone treatment on fat deposition exceeded those which would be expected to accompany their accelerated growth. Excess fat was deposited at the expense of the protein component of fat-free solid.

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