Intracellular formation of calcium concretions by phagocytic cells in freshwater mussels

1989 ◽  
Vol 67 (1) ◽  
pp. 198-207 ◽  
Author(s):  
Harold Silverman ◽  
Pamela E. Richard ◽  
Russell H. Goddard ◽  
Thomas H. Dietz
Keyword(s):  
Connective Tissue ◽  
Colloidal Gold ◽  
Synthetic Activity ◽  
Stereo Pair ◽  
Phagocytic Cells ◽  
India Ink ◽  
Transmission Electron ◽  
Tissue Cells ◽  
Endocytic Activity ◽  
Endocytic Vesicles

The gills of freshwater unionid mussels contain large accumulations of extracellular calcium phosphate concretions. Connective tissue cells located in specific areas in the gills of these animals are the site of concretion formation. The cell type responsible for concretion formation appears to differentiate from an undifferentiated precursor cell by accumulating endocytic vesicles and producing amorphous membrane-bound granules from active rough endoplasmic reticulum and Golgi apparatus. In the most mature of the cells, these granules occupy most of the cytoplasm. Concretions are initiated within the amorphous granules as observed using stereo-pair transmission electron microscopy. These connective tissue cells exhibit phagocytosis, and can ingest India ink particles and colloidal gold. Colloidal gold is internalized through an endocytic mechanism within vesicles. Gold can be observed in the amorphous granules, implying fusion of such endocytic vesicles with the amorphous granules. While the exact mechanism of concretion formation is not understood, the cellular site of formation has been identified. Further, protein synthetic activity as well as endocytic activity appear necessary for concretion production.

Ultrastructural studies of the relationship between actin filaments and secretory granules

1990 ◽  
Vol 48 (3) ◽  
pp. 458-459
Author(s):  
Ellen Holm Nielsen
Keyword(s):  
Electron Microscopy ◽  
Colloidal Gold ◽  
Actin Filaments ◽  
Secretory Granules ◽  
Secretory Cells ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Granule Membrane ◽  
Ultrathin Sections ◽  
Lowicryl K4m

In secretory cells a dense and complex network of actin filaments is seen in the subplasmalemmal space attached to the cell membrane. During exocytosis this network is undergoing a rearrangement facilitating access of granules to plasma membrane in order that fusion of the membranes can take place. A filamentous network related to secretory granules has been reported, but its structural organization and composition have not been examined, although this network may be important for exocytosis.Samples of peritoneal mast cells were frozen at -70°C and thawed at 4°C in order to rupture the cells in such a gentle way that the granule membrane is still intact. Unruptured and ruptured cells were fixed in 2% paraformaldehyde and 0.075% glutaraldehyde, dehydrated in ethanol. For TEM (transmission electron microscopy) cells were embedded in Lowicryl K4M at -35°C and for SEM (scanning electron microscopy) they were placed on copper blocks, critical point dried and coated. For immunoelectron microscopy ultrathin sections were incubated with monoclonal anti-actin and colloidal gold labelled IgM. Ruptured cells were also placed on cover glasses, prefixed, and incubated with anti-actin and colloidal gold labelled IgM.

Specificity of Lectins Labeled with Colloidal Gold to the Exopolymeric Matrix Carbohydrates of the Sulfate-Reducing Bacteria Biofilm Formed on Steel

2020 ◽  
Vol 82 (5) ◽  
pp. 11-20
Author(s):  
D.R. Abdulina ◽  
◽  
L.M. Purish ◽  
G.O. Iutynska ◽  
◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Colloidal Gold ◽  
Steel Surface ◽  
Lectin Binding ◽  
Sulfate Reducing Bacteria ◽  
Gold Particles ◽  
Sulfate Reducing ◽  
Transmission Electron ◽  
Reducing Bacteria

The studies of the carbohydrate composition of the sulfate-reducing bacteria (SRB) biofilms formed on the steel surface, which are a factor of microbial corrosion, are significant. Since exopolymers synthesized by bacteria could activate corrosive processes. The aim of the study was to investigate the specificity of commercial lectins, labeled with colloidal gold to carbohydrates in the biofilm exopolymeric matrix produced by the corrosive-relevant SRB strains from man-caused ecotopes. Methods. Microbiological methods (obtaining of the SRB biofilms during cultivation in liquid Postgate B media under microaerophilic conditions), biochemical methods (lectin-binding analysis of 10 commercial lectins, labeled with colloidal gold), transmission electron microscopy using JEM-1400 JEOL. Results. It was shown using transmission electron microscopy that the binding of lectins with carbohydrates in the biofilm of the studied SRB strains occurred directly in the exopolymerіс matrix, as well as on the surfaces of bacterial cells, as seen by the presence of colloidal gold particles. For detection of the neutral carbohydrates (D-glucose and D-mannose) in the biofilm of almost all studied bacterial strains PSA lectin was the most specific. This lectin binding in biofilms of Desulfotomaculum sp. К1/3 and Desulfovibrio sp. 10 strains was higher in 90.8% and 94.4%, respectively, then for ConA lectin. The presence of fucose in the SRB biofilms was detected using LABA lectin, that showed specificity to the biofilm EPS of all the studied strains. LBA lectin was the most specific to N-аcetyl-D-galactosamine for determination of amino sugars in the biofilm. The amount of this lectin binding in D. vulgaris DSM644 biofilm was 30.3, 10.1 and 9.3 times higher than SBA, SNA and PNA lectins, respectively. STA, LVA and WGA lectins were used to detect the N-acetyl-Dglucosamine and sialic acid in the biofilm. WGA lectin showed specificity to N-acetyl-D-glucosamine in the biofilm of all the studied SRB; maximum number of bounded colloidal gold particles (175 particles/μm2) was found in the Desulfotomaculum sp. TC3 biofilm. STA lectin was interacted most actively with N-acetyl-D-glucosamine in Desulfotomaculum sp. TC3 and Desulfomicrobium sp. TC4 biofilms. The number of bounded colloidal gold particles was in 9.2 and 7.4 times higher, respectively, than using LVA lectin. The lowest binding of colloidal gold particles was observed for LVA lectin. Conclusions. It was identified the individual specificity of the 10 commercial lectins to the carbohydrates of biofilm matrix on the steel surface, produced by SRB. It was estimated that lectins with identical carbohydrates specificity had variation in binding to the biofilm carbohydrates of different SRB strains. Establishing of the lectin range selected for each culture lead to the reduction of the scope of studies and labor time in the researching of the peculiarities of exopolymeric matrix composition of biofilms formed by corrosiverelevant SRB.

Increased matrix gene expression by glucose in rat neural connective tissue cells in culture

Diabetes ◽  
1991 ◽  
Vol 40 (5) ◽  
pp. 605-611 ◽  
Author(s):  
P. Muona ◽  
J. Peltonen ◽  
S. Jaakkola ◽  
J. Uitto
Keyword(s):  
Gene Expression ◽  
Connective Tissue ◽  
Cells In Culture ◽  
Matrix Gene ◽  
Tissue Cells

Histopathology of the A1 Pulley in Adult Trigger Fingers

2007 ◽  
Vol 32 (5) ◽  
pp. 556-559 ◽  
Author(s):  
M. C. SBERNARDORI ◽  
P. BANDIERA
Keyword(s):  
Connective Tissue ◽  
Middle Layer ◽  
Laminar Structure ◽  
Loose Connective Tissue ◽  
Control Series ◽  
Outermost Layer ◽  
Additional Layer ◽  
Transmission Electron ◽  
Electron Microscopes ◽  
A1 Pulley

The histopathology of the central parts of 40 A1 pulleys from adult patients with primary trigger fingers was studied using light and transmission electron microscopes and the findings were compared with those in a control series of 10 normal A1 pulleys. The evaluation of the normal A1 pulley revealed a bi-laminar structure. The deepest layer was composed of dense normal connective tissue. The outermost layer was formed by loose connective tissue. In trigger digits, it was possible to identify a tri-laminar structure. The deepest layer was composed of irregular connective tissue, formed by small collagen fibres and abundant extracellular matrix. A considerable amount of chondroid-metaplasia was present in this layer. The middle layer contained dense, normal connective tissue with some fibrocytes. The outermost layer was formed of loose connective tissue. In conclusion, there was an additional layer in the A1 pulley in pathological cases which was not present in normal pulleys.

Effect of Voltage on Colloidal Gold (Au) Nanoparticles Produced Using Electro-Dissolution-Reduction Method

2015 ◽  
Vol 1115 ◽  
pp. 386-389
Author(s):  
Haroon Haiza ◽  
I.I. Yaacob ◽  
Ahmad Zahirani Ahmad Azhar
Keyword(s):  
Gold Nanoparticles ◽  
Electron Microscope ◽  
Colloidal Gold ◽  
Reduction Process ◽  
Chloride Ions ◽  
Complex Ions ◽  
Dc Power ◽  
Colloidal Gold Nanoparticles ◽  
Transmission Electron ◽  
The Mean

Colloidal gold nanoparticles have been successfully prepared using a simple two-electrode cells connected to a DC power supply. During the electro-dissolution-reduction process, the bulk gold at the anode oxidized into gold cations which then reacted with the chloride ions to form aurochloride complex. The complex ions were then reduced by the citrate ion to form colloidal gold nanoparticles. The size and shape of the nanoparticles were modulated by varying the terminal voltages. The colloidal gold nanoparticles obtained were characterized by field-emission scanning electron microscope (FESEM), transmission electron microscope (TEM) and ultraviolet-visible spectrophotometer (UV-Vis). From FESEM analysis, it was found that by increasing the voltage, the size of colloidal gold nanoparticles produced marginally decreased. The mean sizes of gold nanoparticles were roughly about 23.5 nm, 23.2 nm and 19.3 nm for 32 V, 36 V and 40 V, respectively. TEM micrograph showed that the shape of gold nanoparticles obtained is almost spherical. The characteristic peaks of UV-Vis spectra revealed that the suspension was indeed colloidal gold nanoparticles. Keywords: Gold, Nanoparticles, Electro-dissolution-reduction

scholarly journals Immunocytochemical characteristics of submucosal uterine myomas

2010 ◽  
Vol 67 (12) ◽  
pp. 977-982 ◽  
Author(s):  
Aleksandra Mladenovic-Mihailovic ◽  
Zorica Mladenovic-Bogdanovic ◽  
Predrag Mitrovic ◽  
Irena Tanaskovic ◽  
Slavica Usaj-Knezevic ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Connective Tissue ◽  
Smooth Muscle Cells ◽  
Smooth Muscle Actin ◽  
Muscle Cells ◽  
Synthetic Activity ◽  
Benign Tumors ◽  
Paraffin Sections ◽  
Weak Reaction ◽  
Synthetic Phenotype

Background/Aim. Myomas of the uterus, the most common benign tumors, have been studied for decades from the aspects of different basic and clinical disciplines. Despite this fact, their pathogenesis is still poorly understood. The aim of this study was to determine immunocytochemical characteristics of smooth muscle cells and connective tissue components of submucosal myomas of the uterus. Method. During the course of this study, 25 samples of submucosal myomas of the uterus were analyzed, all of them obtained during the surgery, after abdominal histerctomy by Aldridge. The samples were fixed in 4% formalin and embedded in paraffin. Sections of 5 ?m thickness were stained immunocytochemically using the DAKO LSAB+/HRP technique to identify ?- smooth muscle actin (?-SMA), vimentin, desmin, CD34, CD45, CD68 and PCNA (DAKO specification). Results. Our results suggest that submucosal myomas of the uterus are build-up of smooth muscle cells which are immunoreactive to ?-SMA and desmin, but also to a certain number of smooth muscle cells which are immunoreactive to ?-SMA and vimentin. Some of vimentin-immunoreactive cells also show an immunoreactivity of PCNA. In the build-up of connective stroma CD34-immunoreactive fibroblasts and neovascular formations are also present. By examining the distribution of CD45 antigen, at all the analyzed samples we observed a weak reaction. Conclusion. Submucosal myomas of the uterus are made-up of smooth muscle cells of the highly differentiated contractile phenotype (?-SMA- and desminimmunoreactivity), as well as smooth muscle cell of the synthetic phenotype which proliferate (?-SMA-, vimentin- and PCNA-immunoreactivity). In submucosal myoma of the uterus there is a significant presence of connective tissue as a result of synthetic activity of fibroblasts, which clearly differ in their immunocytochemical characteristics from smooth muscle cells of the synthetic phenotype.

scholarly journals AUTORADIOGRAPHIC LOCALIZATION OF NEW RNA SYNTHESIS IN HYPERTROPHYING SKELETAL MUSCLE

1973 ◽  
Vol 57 (3) ◽  
pp. 743-759 ◽  
Author(s):  
Charles K. Jablecki ◽  
John E. Heuser ◽  
Seymour Kaufman
Keyword(s):  
Skeletal Muscle ◽  
Connective Tissue ◽  
Rna Synthesis ◽  
Proliferating Cells ◽  
Quantitative Studies ◽  
Physiological Adaptations ◽  
Rat Soleus Muscle ◽  
Tissue Cells ◽  
A Site ◽  
Silver Grains

Work-induced growth of rat soleus muscle is accompanied by an early increase in new RNA synthesis. To determine the cell type(s) responsible for the increased RNA synthesis, we compared light autoradiographs of control and hypertrophying muscles from rats injected with tritiated uridine 12, 24, and 48 h after inducing hypertrophy. There was an increased number of silver grains over autoradiographs of hypertrophied muscle. This increase occurred over connective tissue cells; there was no increase in the number of silver grains over the muscle fibers. Quantitative studies demonstrated that between 70 and 80% of the radioactivity in the muscle that survived fixation and washing was in RNA. Pretreatment of the animals with actinomycin D reduced in parallel both the radioactivity in RNA and the number of silver grains over autoradiographs. Proliferation of the connective tissue in hypertrophying muscle was evident in light micrographs, and electron micrographs identified the proliferating cells as enlarged fibroblasts and macrophages; the connective tissue cells remained after hypertrophy was completed. Thus, proliferating connective tissue cells are the major site of the increase in new RNA synthesis during acute work-induced growth of skeletal muscle. It is suggested that in the analysis of physiological adaptations of muscle, the connective tissue cells deserve consideration as a site of significant molecular activity.

scholarly journals THE COMPARATIVE RESISTANCE OF BACTERIA AND HUMAN TISSUE CELLS TO CERTAIN COMMON ANTISEPTICS

1916 ◽  
Vol 24 (6) ◽  
pp. 683-688 ◽  
Author(s):  
Robert A. Lambert
Keyword(s):  
Hydrogen Peroxide ◽  
Staphylococcus Aureus ◽  
Connective Tissue ◽  
Human Tissue ◽  
Tissue Cultures ◽  
Mercuric Iodide ◽  
Living Body ◽  
Tissue Cells ◽  
The One ◽  
The Ideal

The comparative resistance of bacteria and human tissue cells to antiseptics and other chemicals may be easily tested by tissue cultures under conditions which approximate those found in the living body. A comparative study shows that while human cells (connective tissue and wandering cells) are highly resistant to many antiseptics, they are in general more easily killed than bacteria (Staphylococcus aureus). Of the antiseptics tested, which include mercuric chloride, iodine, potassium mercuric iodide, phenol, tricresol, hydrogen peroxide, hypochlorites (Dakin's solution), argyrol, and alcohol, the one which approaches most closely the ideal disinfectant is iodine, which kills bacteria in strengths that do not seriously injure connective tissue cells or wandering cells.

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