An investigation of host influence on soluble protein banding profiles of Hymenolepis spp. (Cestoidea), using isoelectric focusing

1987 ◽  
Vol 65 (10) ◽  
pp. 2471-2474 ◽  
Author(s):  
Brent R. Dixon ◽  
Hisao P. Arai
Keyword(s):  
Isoelectric Focusing ◽  
Definitive Host ◽  
Host Species ◽  
Soluble Protein ◽  
Protein Separation ◽  
Soluble Proteins ◽  
Banding Patterns ◽  
Morphological Approach ◽  
Alternative Means ◽  
Host Influence

Protein separation techniques have been utilized in recent years as alternative means of differentiating species and strains of tapeworms. As with the traditional morphological approach, however, there is the potential for host influence. For such separative techniques to be useful taxonomically it is important that the banding profiles differentiating parasites be consistent, regardless of host species. To test for host influence, two Hymenolepis species were maintained both in preferred and alternative definitive host species. The soluble proteins of tapeworms of a given species, from the different host species, were then subjected to isoelectric focusing, and the resulting banding patterns were compared. There were no noticeable differences in the banding profiles of these worms, indicating a lack of host influence. If these findings hold for other tapeworm genera, isoelectric focusing of soluble proteins could have practical value in parasite surveys.

Isoelectric focusing of soluble proteins in the characterization of three species of Hymenolepis (Cestoda)

1985 ◽  
Vol 63 (7) ◽  
pp. 1720-1723 ◽  
Author(s):  
Brent R. Dixon ◽  
Hisao P. Arai
Keyword(s):  
Isoelectric Focusing ◽  
Protein Separation ◽  
Soluble Proteins ◽  
Hymenolepis Diminuta ◽  
Good Resolution ◽  
Banding Patterns ◽  
Morphological Approach ◽  
Single Host ◽  
Taxonomic Studies

A technique involving protein separation was used as an alternative to a morphological approach in the differentiation of the tapeworms Hymenolepis diminuta, H. citelli, and H. microstoma. Isoelectric focusing of soluble proteins was performed on Polyacrylamide gels using extracts from whole, adult worms. Each species of Hymenolepis was found to have a unique protein banding pattern, although some bands appeared to be common to two or all three species. Very little difference was found in the protein banding patterns of worms of a given species, whether they were from a single host individual or two different host individuals of the same species. There was also little difference between gels in the banding patterns of a given species. This technique of soluble protein isoelectric focusing is simple and reproducible, has very good resolution, and seems well suited to taxonomic studies involving tapeworms.

Interspecific characterization of several taeniid cestodes by isoenzyme analysis using isoelectric focusing in agarose

Parasitology ◽  
1987 ◽  
Vol 95 (3) ◽  
pp. 593-601 ◽  
Author(s):  
B. A. Allsopp ◽  
A. Jones ◽  
M. T. E. P. Allsopp ◽  
S. D. Newton ◽  
C. N. L. Macpherson
Keyword(s):  
Isoelectric Focusing ◽  
Definitive Host ◽  
Host Species ◽  
Isoenzyme Analysis ◽  
Agarose Gels ◽  
Identification Of Species

SUMMARYTaenia cestodes were obtained from 5 different definitive host species in Kenya and 175 different samples were examined by classical morphological methods and by isoenzyme analysis using isoelectric focusing in agarose. Gels were stained for 17 different enzymes and 3 of these were used in the construction of isoenzyme profiles. The samples fell into 25 zymodemes, and no zymodeme contained more than 1 species of Taenia, indicating that isoenzyme analysis can reliably be used for the identification of species of this genus.

scholarly journals PAG electrophoregrams of six Finnish potato cultivars

1985 ◽  
Vol 57 (3) ◽  
pp. 147-154
Author(s):  
Tuula Sontag ◽  
Hannu Salovaara ◽  
Osmo Ulvinen
Keyword(s):  
Soluble Protein ◽  
Protein Separation ◽  
Electrophoretic Pattern ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Potato Cultivars ◽  
Good Resolution ◽  
Local Cultivars ◽  
Close Relatives

The polyacrylamide gel electrophoretic (PAGE) patterns of soluble proteins and esterases of six Finnish potato cultivars (Jaakko, Pito, Hankkijan Timo, Hankkijan Tuomas, Hankkijan Tanu and Puikula) were determined. All cultivars are commonly grown in Finland. The PAGE procedure used yielded highly reproducible protein separation and good resolution. Samples studied had specific soluble protein and esterase PAGE patterns, indicating that electrophoregrams can be used for identifying Finnish potato cultivars. Only two cultivars, Hankkijan Tanu and Hankkijan Tuomas, which are close relatives, possessed very similar PAGE patterns. The electrophoretic pattern of Puikula was very similar to that of the Swedish cultivar Mandel when compared with the reference presented in the literature.Therefore a hypothesis is presented suggesting that these two local cultivars would be representatives of the same cultivar.

Epizoic diatoms on sea turtles and their relationship to host species, behaviour and biogeography: a morphological approach

2021 ◽  
pp. 1-14
Author(s):  
Catherine Riaux-Gobin ◽  
Matt P. Ashworth ◽  
J.Patrick Kociolek ◽  
Damien Chevallier ◽  
Pablo Saenz-Agudelo ◽  
...  
Keyword(s):  
Host Species ◽  
Sea Turtles ◽  
Morphological Approach

scholarly journals GENETICALLY CONTROLLED VARIATION OF "ACID" β-GALACTOSIDASE DETECTED IN Rattus norvegicus BY ISOELECTRIC FOCUSING

Genetics ◽  
1982 ◽  
Vol 100 (3) ◽  
pp. 455-473
Author(s):  
Tommy C Douglas ◽  
Kathryn A Kimmel ◽  
Patti E Dawson
Keyword(s):  
Isoelectric Focusing ◽  
Rattus Norvegicus ◽  
Rat Kidney ◽  
Ph Dependence ◽  
Point Mutations ◽  
Autosomal Locus ◽  
Ph Optimum ◽  
Banding Patterns ◽  
Isoelectric Points ◽  
Significant Linkage

ABSTRACT Two genetically variant forms of rat "acid" β-galactosidase were found to differ in isoelectric point and pH dependence, but not in thermostability or sensitivity to inhibition by p-mercuribenzoate (PMB). The results of two backcrosses and an intercross indicated that the isoelectric focusing phenotypes are controlled by two codominant alleles at a single autosomal locus, for which we propose the name Glb-1. No significant linkage between Glb-1 and albino (LG I), brown (LG II), or hooded (LG VI) was observed. Strain-specific differences in total levels of kidney β-galactosidase were detected, but it is not yet known whether the variation is controlled by genes linked to Glb-1. Experiments in which organ homogenates were incubated with neuraminidase indicated that the genetically variant forms do not result from differences in sialylation, though sialylation does appear to be largely responsible for the presence of multiple bands within each phenotype and for differences in the banding patterns of β-galactosidases derived from different organs. The β-galactosidase present in the bands used for Glb-1 typing resembles human GM1 gangliosidase (GLB1) with respect to pH optimum, substrate specificity, and susceptibility to inhibition by PMB. It also appears that Glb-1 is homologous with the Bgl-e locus of the mouse. In rats as in mice the genetically variant bands of β-galactosidase are active at acid pH and have relatively high isoelectric points. In both species these bands are readily detectable in kidney homogenates, and can be revealed in homogenates of liver or spleen following treatment with neuraminidase. The presence of the same β-galactosidase bands in homogenates of rat kidney and small intestine as well as in neuraminidase-treated homogenates of liver and spleen suggests that the Glb-1 variants differ by one or more point mutations in the structural gene for "acid" β-galactosidase.

The influence of second intermediate host species on the infectivity of metacercarial cysts of Echinoparyphium recurvatum

1999 ◽  
Vol 73 (2) ◽  
pp. 143-145 ◽  
Author(s):  
A.M. McCarthy
Keyword(s):  
Intermediate Host ◽  
Definitive Host ◽  
Host Species ◽  
Post Infection ◽  
Rana Temporaria ◽  
Biomphalaria Glabrata ◽  
Reproductive Status ◽  
Intermediate Hosts ◽  
Potential Influence ◽  
Second Intermediate Host

The potential influence of second intermediate host species on the infectivity of metacercarial cysts of Echinoparyphium recurvatum to the definitive host Anas platyrhynchos was examined experimentally. Echinoparyphium recurvatum metacercarial cysts were obtained from the following experimentally infected second intermediate hosts 14 days post expsoure to cercariae: Lymnaea peregra; Physa fontinalis; L. stagnalis;Planorbis planorbis; Biomphalaria glabrata; tadpoles of the amphibian Rana temporaria. Metacercarial cysts from each of these hosts were fed, in doses of 50 cysts per individual, to separate groups composed of between four and eight, 3-day-old A. platyrhynchos ducklings. All A. platyrhynchos were necropsied 15 days post-infection and the number, size, and reproductive status of E. recurvatum worms in the intestine was recorded. Analyses of variance on the number (transformed log (x + 1)) and size of worms revealed no significant differences in worms originating from metacercariae formed in the different second intermediate hosts (worm number P > 0.05, and worm size P > 0.05). All worms recovered were found to be gravid. It is therefore concluded that the species of second intermediate host utilized does not influence the infectivity of the metacercarial cyst of E. recurvatum, nor the subsequent establishment and reproductive status of the parasite in A. platyrhynchos.

Comparative migration, growth and development of Typhlocoelum cucumerinum sisowi in dabbling ducks and Typhlocoelum cucumerinum cucumerinum in diving ducks

Parasitology ◽  
1982 ◽  
Vol 84 (2) ◽  
pp. 333-350 ◽  
Author(s):  
Marilyn E. Scott ◽  
M. E. Rau ◽  
J. D. McLaughlin
Keyword(s):  
Life Cycle ◽  
Life Span ◽  
Definitive Host ◽  
Host Species ◽  
Growth And Development ◽  
Abdominal Cavity ◽  
Dabbling Ducks ◽  
Diving Ducks ◽  
Air Sacs ◽  
Aythya Valisineria

SUMMARYExperimental infections of mallards (Anas platyrhynchos L.) with Typhlocoelum cucumerinum sisowi (Skrjabin, 1913) and of canvasbacks (Aythya valisineria (Wilson)) with Typhlocoelum cucumerinum cucumerinum (Rudolphi, 1809) revealed significant differences in various parameters of the life-cycle in the definitive host. Both T. c. sisowi and T. c. cucumerinum migrate to the trachea via the abdominal cavity, air sacs and lungs, although T. c. cucumerinum migrate more quickly and more synchronously than T. c. sisowi. Typhlocoelum c. sisowi has a shorter expected life-span than T. c. cucumerinum but grows and reaches maturity more quickly than T. c. cucumerinum. Evidence suggests that T. c. cucumerinum has a higher fecundity than T. c. sisowi. These differences in the patterns of migration, growth and development are related not only to differences between the two host species but also to differences intrinsic to the parasites, and serve to provide biological support for considering them as separate sub-species.

Improved protein separation by microchip isoelectric focusing with stepwise gradient of electric field strength

2009 ◽  
Vol 32 (3) ◽  
pp. 462-465 ◽  
Author(s):  
Yongzheng Cong ◽  
Yu Liang ◽  
Lihua Zhang ◽  
Weibing Zhang ◽  
Yukui Zhang
Keyword(s):  
Field Strength ◽  
Electric Field ◽  
Electric Field Strength ◽  
Isoelectric Focusing ◽  
Protein Separation ◽  
Stepwise Gradient
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