Electrophoretic divergence of Myotis leibii and Myotis ciliolabrum (Chiroptera: Vespertilionidae)

1987 ◽  
Vol 65 (7) ◽  
pp. 1857-1860 ◽  
Author(s):  
Robert M. Herd
Keyword(s):  
Distinct Species ◽  
Data Support ◽  
Electrophoretic Data ◽  
Allelic Differences ◽  
Myotis Leibii

Myotis ciliolabrum has recently been recognised as a species distinct from Myotis leibii. Electrophoretic divergence between the two species exceeds that of subspecies of other bats and is similar to that found between other species. Fixed allelic differences at two loci suggested that no introgression occurs. The electrophoretic data support the conclusion that M. ciliolabrum and M. leibii are distinct species.

scholarly journals Electrophoretic Resolution of Species Boundaries in Australian Microchiroptera. IV. The Molossidae (Chiroptera)

1988 ◽  
Vol 41 (3) ◽  
pp. 315 ◽  
Author(s):  
M Adams ◽  
TR Reardon ◽  
PR Baverstock ◽  
CHS Watts
Keyword(s):  
Species Level ◽  
Species Boundaries ◽  
Allozyme Electrophoresis ◽  
Single Specimen ◽  
Data Support ◽  
Electrophoretic Data ◽  
Current Species ◽  
Electrophoretic Resolution

Allozyme electrophoresis of 35 loci in 156 specimens of Australian bats belonging to the Molossidae was used to help elucidate the species-level taxonomy of the group in Australia. The electrophoretic data support the current species-level taxonomy of Tadarida australis and Chaerephon jobensis. However, for specimens currently allocated to the genus Mormopterus, the electrophoretic data fail to support any previous species-level account. On the electrophoretic data, a minimum of five species of the genus Mormopterus occur in Australia. A single specimen of a sixth species, whose generic affinities are undetermined, was also found.

Electrophoretic contributions to the systematics of the freshwater crayfish genus Engaeus Erichson (Decapoda : Parastacidae)

1990 ◽  
Vol 4 (3) ◽  
pp. 615 ◽  
Author(s):  
P Horwitz ◽  
M Adams ◽  
P Baverstock
Keyword(s):  
Allelic Variation ◽  
Threshold Level ◽  
Sympatric Species ◽  
Biological Species ◽  
Genetic Differences ◽  
Freshwater Crayfish ◽  
Electrophoretic Data ◽  
Allelic Differences ◽  
Low Levels ◽  
Group Relationships

As part of an examination into the systematics of the freshwater crayfish genus Engaeus, an electrophoretic evaluation of specimens from a large number of collecting sites has been undertaken. From a total of 76 sample sets, a minimum of 30 distinct biological species have been delineated. Strong evidence from sites of both sympatry and allopatry suggests some further delineations of species but these must await additional discriminatory information. Sympatric species were detected on 11 separate occasions. In each case, fixed allelic differences were found, indicating an absence of interbreeding between each sympatric pair. Allopatric species were delineated where genetic differences between populations exceeded a threshold level determined from observed genetic differences between sympatric Engaeus species and between other (mainly decapod) species in the literature. The results of this analysis have confirmed low levels of heterozygosity in Engaeus species. Frequent isoIation of small populations of Engaeus species, and consequent loss of allelic variation, is suggested as a possible cause of these low levels. Some species have shown variation of allelic frequencies over their geographical range and, in some cases, this variation could be interpreted as showing clinal properties. Finally, the electrophoretic data have been interpreted to produce some information regarding the phylogenetic affinities of delineated species. Several groups of species were found which shared closer intra-group relationships than they did to the other species of Engaeus.

An electrophoretic comparison of the Australian paralysis tick, Ixodes holocyclus Neumann, 1899, with I. cornuatus Roberts, 1960 (Acari : Ixodidae)

1998 ◽  
Vol 46 (2) ◽  
pp. 109 ◽  
Author(s):  
Janey Jackson ◽  
Neil B. Chilton ◽  
Ian Beveridge ◽  
Michelle Morris ◽  
Ross H. Andrews
Keyword(s):  
Cryptic Species ◽  
Genetic Heterogeneity ◽  
Genetic Divergence ◽  
Distinct Species ◽  
Morphological Characters ◽  
Population Variation ◽  
Electrophoretic Study ◽  
Ixodes Holocyclus ◽  
Electrophoretic Data ◽  
Paralysis Tick

An electrophoretic study was conducted on ticks morphologically identified as either Ixodes cornuatus or I. holocyclus from Tasmania and several localities on mainland Australia. Ticks were characterised genetically at 27 enzyme loci encoding 24 enzymes. The extent of genetic divergence (52–69% fixed differences) between ticks from Tasmania and those from the mainland was equivalent to that found between these two groups and two morphologically distinct species, I. hirsti and I. fecialis. The Tasmanian ticks therefore represent a different species from the specimens from the mainland and the electrophoretic data show that the morphological characters currently used to distinguish I. holocyclus from I. cornuatus are inadequate. Genetic heterogeneity was detected in samples from different localities on the mainland. This could represent either population variation, or the existence of cryptic species, but more ticks from these mainland localities need to be examined electrophoretically to resolve this.

scholarly journals Molecular systematics of New Zealand Cyanoramphus parakeets: conservation of Orange-fronted and Forbes' Parakeets

2000 ◽  
Vol 10 (3) ◽  
pp. 211-239 ◽  
Author(s):  
W. M. Boon ◽  
J. C. Kearvell ◽  
C. H. Daugherty ◽  
G. K. Chambers
Keyword(s):  
Dna Sequences ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
Biological Species ◽  
Biological Species Concept ◽  
Allozyme Data ◽  
Dna Sequence Data ◽  
Data Support ◽  
High Conservation

The controversy that presently surrounds the taxonomy of the Orange-fronted Cyanoramphus malherbi and Forbes' C. forbesi Parakeets has important implications for the conservation of both birds. Both taxa are critically endangered, but consensus regarding their specific status has not yet been achieved. We present mitochondrial DNA sequences for the cytochrome b gene and the control region from 17 Cyanoramphus parakeets representing nine populations and six taxa together with field observations of courtship and breeding behaviour in a sympatric population of Orange-fronted and Yellow-crowned Parakeets C. auriceps. Field data support species status of the Orange-fronted Parakeet under the Biological Species Concept. Phylogenetic analyses of our DNA sequence data support earlier hypotheses based on allozyme data that both Orange-fronted and Forbes' Parakeets represent distinct species under four species concepts and indicate that high conservation priority is warranted for both taxa.

Morphological, electrophoretic, and ecological analysis of Quercus macrocarpa populations in the Black Hills of South Dakota and Wyoming

1990 ◽  
Vol 68 (10) ◽  
pp. 2185-2194 ◽  
Author(s):  
Naomi Chechowitz ◽  
Dorothy M. Chappell ◽  
Sheldon I. Guttman ◽  
Lee A. Weigt
Keyword(s):  
Natural Selection ◽  
South Dakota ◽  
Introgressive Hybridization ◽  
Black Hills ◽  
Morphological Data ◽  
Ecological Analysis ◽  
Single Population ◽  
Quercus Macrocarpa ◽  
Data Support ◽  
Electrophoretic Data

Morphological data support the hypothesis that oak populations in the Black Hills of South Dakota and Wyoming reflect the effect of introgressive hybridization between Quercus macrocarpa and Q. gambelii; these populations cannot be electro-phoretically differentiated from Q. macrocarpa. Thirteen populations of oaks were sampled in the Black Hills of South Dakota and Wyoming, and a single population each of Q. macrocarpa and Q. gambelii were sampled outside of the Black Hills. Morphological data suggest significant similarities of populations located in northwestern Black Hills with Q. gambelii and populations located in the eastern Black Hills with Q. macrocarpa. Disparity between morphological and electrophoretic data suggests that natural selection is operating differently on the morphological and isozymic characters.

DNA barcoding, MALDI-TOF, and AFLP data support Fusarium ficicrescens as a distinct species within the Fusarium fujikuroi species complex

2016 ◽  
Vol 120 (2) ◽  
pp. 265-278 ◽  
Author(s):  
Abdullah M.S. Al-Hatmi ◽  
Mansoureh Mirabolfathy ◽  
Ferry Hagen ◽  
Anne-Cécile Normand ◽  
J. Benjamin Stielow ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Species Complex ◽  
Distinct Species ◽  
Fusarium Fujikuroi ◽  
Aflp Data ◽  
Maldi Tof ◽  
Data Support ◽  
Fusarium Fujikuroi Species Complex

Apparent absence of genetic differences among species of Teladorsagia (Nematoda: Trichostrongylidae)

1990 ◽  
Vol 64 (4) ◽  
pp. 290-294 ◽  
Author(s):  
R. H. Andrews ◽  
I. Beveridge
Keyword(s):  
Genetic Variation ◽  
Genetic Data ◽  
Single Species ◽  
Genetic Differences ◽  
Morphological Evidence ◽  
Allozyme Electrophoresis ◽  
Apparent Absence ◽  
Enzyme Loci ◽  
Data Support ◽  
Allelic Differences

ABSTRACTThe technique of allozyme electrophoresis was applied to three species of Teladorsagia present in sheep to determine the extent of genetic variation among species. Thirty-four enzyme loci were established of which 22 were invariant and 12 were shown to be polymorphic. No fixed allelic differences were detected among the species and the level of polymorphism was within the range found commonly between populations of a single species. Therefore, the genetic data support breeding data and existing morphological evidence that T. circumcincta, T. davtiani and T. trifurcata as currently recognized do in fact belong to a single species.

Liparis meihuashanensis, a new orchid species from Fujian, China: Evidence from morphological and molecular analyses

Phytotaxa ◽  
2017 ◽  
Vol 323 (2) ◽  
pp. 182
Author(s):  
SHI-MING FAN ◽  
JIANG-FENG LIU ◽  
JUN-WEN ZHAI ◽  
CHENG-ZI YANG ◽  
ZE-HAO HUANG
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Distinct Species ◽  
Floral Bract ◽  
Orchid Species ◽  
Molecular Analyses ◽  
Dna Sequence Data ◽  
Data Support ◽  
Nuclear Its

A new orchid species, Liparis meihuashanensis, from Fujian, China is described and illustrated based on morphological and molecular analyses. Detailed morphological comparisons indicate that L. meihuashanensis is similar to L. auriculata and L. pauliana, but it can be distinguished from them by the shorter inflorescence, triangular floral bract, and a lip with a truncate-emarginate and mucronate apex and 2 small subconical calli on contracted base. Molecular analyses based on nuclear ITS and plastid matK DNA sequence data support the L. meihuashanensis as a distinct species.

Ultrastructural and chemical evidences of heterogeneity and hormone dependence of certain glycocalyces

1978 ◽  
Vol 36 (2) ◽  
pp. 322-323
Author(s):  
B. Monis ◽  
D. Lis ◽  
I. Parlanti ◽  
A. R. Eynard ◽  
M. A. Valentich ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Concanavalin A ◽  
Ruthenium Red ◽  
Transitional Epithelium ◽  
Membrane Material ◽  
Hormone Dependence ◽  
Cellulose Acetate Electrophoresis ◽  
Electrophoretic Data ◽  
Fat Globule ◽  
Collecting Tubules

We are gathering evidences which indicate ultrastructural variations and chemical heterogeneity of certain glycocalyces as well as hormone dependence of some of them. Thus, in the lumenal glycocalyx of renal collecting tubules of the guinea-pig granular and filamentous structures were seen (1, fig. 1). By isolation, chemical analysis and cellulose acetate electrophoresis in various buffers of tubular membrane material, glycopeptides and glycosaminoglycans were identified (fig. 2).Guinea-pig and rat transitional epithelium of urinary tract showed a filamentous lumenal glycocalyx demonstrable with ruthenium red (fig. 3) but which only in part stained with concanavalin A. Chemical and electrophoretic data indicated that urothelium contains glycoproteins, glycosaminoglycans and glycolipids.The glycocalyx of the fat globule membrane of milk of several species has a granular appearance as shown by cationic dyes and by concanavalin A (2, 3, fig. 4 and 5). Also, several glycoproteins were isolated and identified on polyacrilamide gel electrophoresis (fig. 6). Glycosaminoglycans and certain glycolipids such as sulfatides were chemically identified in this glycocalyx.

Discrimination of monomer from multimer DNA disk-shaped toruses by freezeetch TEM

1984 ◽  
Vol 42 ◽  
pp. 210-211
Author(s):  
George C. Ruben ◽  
Kenneth A. Marx
Keyword(s):  
Biochemical Data ◽  
Dna Structures ◽  
Ice Surface ◽  
Double Stranded Dna ◽  
Data Support ◽  
Dna Organization ◽  
Trivalent Cations

In vitro collapse of DNA by trivalent cations like spermidine produces torus (donut) shaped DNA structures thought to have a DNA organization similar to certain double stranded DNA bacteriophage and viruses. This has prompted our studies of these structures using freeze-etch low Pt-C metal (9Å) replica TEM. With a variety of DNAs the TEM and biochemical data support a circumferential DNA winding model for hydrated DNA torus organization. Since toruses are almost invariably oriented nearly horizontal to the ice surface one of the most accessible parameters of a torus population is annulus (ring) thickness. We have tabulated this parameter for populations of both nicked, circular (Fig. 1: n=63) and linear (n=40: data not shown) ϕX-174 DNA toruses. In both cases, as can be noted in Fig. 1, there appears to be a compact grouping of toruses possessing smaller dimensions separated from a dispersed population possessing considerably larger dimensions.

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