Tissue regeneration in the amputated forelimb of Xenopus laevis froglets

1984 ◽  
Vol 62 (12) ◽  
pp. 2383-2391 ◽  
Author(s):  
Robert G. Korneluk ◽  
Richard A. Liversage
Keyword(s):  
Wound Healing ◽  
Xenopus Laevis ◽  
Tissue Regeneration ◽  
Limb Amputation ◽  
Notophthalmus Viridescens ◽  
Epimorphic Regeneration ◽  
Cell Accumulation ◽  
Fibroblastlike Cell ◽  
Complete Regeneration ◽  
Newt Limb

The present study directly compares the response of amputated forelimbs in Xenopus laevis froglets with epimorphic regeneration of forelimbs in adult newts (Notophthalmus viridescens). Epidermal wound healing was initiated following limb amputation in both froglets and newts. Dedifferentiation of mesodermal stump tissues was comparatively extensive in the regenerating newt limb, whereas Xenopus forelimbs underwent little if any tissue dedifferentiation. Blastema accumulation and growth are prominent features of newt forelimb regeneration. In contrast, only a small fibroblastlike cell accumulation was observed at the distal tip of the Xenopus limbs. Differentiation of blastemal cells in the urodele limb was delayed until extensive blastema growth was achieved, whereas differentiation was immediate in Xenopus. Morphogenesis of the regenerative outgrowth in Xenopus was limited to the differentiation of connective tissue elements, primarily cartilage. In contrast, complete regeneration of all limb tissues occurred in newt forelimbs. We conclude from our findings that a dominant tissue regenerative response exists following forelimb amputation in Xenopus froglets, whereas epimorphic regeneration prevails in the amputated adult newt limb.

scholarly journals Prx-1 Expression in Xenopus laevis Scarless Skin-Wound Healing and Its Resemblance to Epimorphic Regeneration

2011 ◽  
Vol 131 (12) ◽  
pp. 2477-2485 ◽  
Author(s):  
Hitoshi Yokoyama ◽  
Tamae Maruoka ◽  
Akio Aruga ◽  
Takanori Amano ◽  
Shiro Ohgo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Xenopus Laevis ◽  
Skin Wound ◽  
Skin Wound Healing ◽  
Epimorphic Regeneration

Role of prostaglandins in amphibian limb regeneration

1981 ◽  
Vol 59 (1) ◽  
pp. 136-137
Author(s):  
Steven R. Scadding
Keyword(s):  
Wound Healing ◽  
Acetylsalicylic Acid ◽  
Limb Regeneration ◽  
Limb Amputation ◽  
Prostaglandin E ◽  
Notophthalmus Viridescens ◽  
Bufo Americanus ◽  
Histological Appearance

Following limb amputation in the newt (Notophthalmus viridescens), the animals were given daily intraperitoneal injections of 0.1 or 1.0 mg of either indomethacin or acetylsalicylic acid. This had no observable influence on the rate or extent of limb regeneration or the histological appearance of the regenerating limb when compared with controls. Following limb amputation in toads (Bufo americanus), the limb stumps were injected daily for the first 15 days after amputation with 5 μg of either prostaglandin E1 or prostaglandin E2. Neither of these treatments had any observed effect on subsequent wound healing of the limbs when compared with controls. These observations suggest that prostaglandins do not play a major role in control of amphibian limb regeneration.

Ultrastructure of Xenopus laevis forelimbs: effects of denervation and delayed amputation

1990 ◽  
Vol 68 (1) ◽  
pp. 53-62
Author(s):  
Richard A. Liversage ◽  
Paul A. Khan
Keyword(s):  
Extracellular Matrix ◽  
Xenopus Laevis ◽  
Tissue Regeneration ◽  
Scar Tissue ◽  
Limb Amputation ◽  
Matrix Deposition ◽  
Ultrastructural Studies ◽  
Peripheral Innervation ◽  
Epithelial Wound Healing ◽  
Extracellular Matrix Deposition

Ultrastructural studies were carried out on left forelimbs of postmetamorphic Xenopus laevis froglets which had been denervated 1 week prior to limb amputation. Limbs were fixed at intervals of 3, 9, and 15 days postamputation. In control and sham-denervated series limbs in which peripheral (brachiospinal) nerves remained intact, tissue regeneration of the amputated forelimb ensued. Extracellular matrix deposition was observed to be minimal among the cells that accumulate subjacent to the wound epithelium of the control and sham cases, not unlike that observed in intact limbs. However, regeneration was not observed in denervated limbs. In denervated forelimbs 9 and 15 days postamputation, deposition of extracellular matrix surrounding the limited fibroblast or fibroblast-like cellular accumulation in the amputated limb stumps was considerably greater than was observed in normally innervated cases. Presumably, extracellular matrix binds fibroblasts together to form scar tissue (cicatrix) which leads to stumping of a denervated, amputated limb. The fibroblasts become narrower, fusiform, and more elongate in denervated nonregenerating forelimbs by 15 days postamputation compared with controls. In all cases fixed 3 days postamputation, epithelial wound healing appeared to proceed normally in the absence of peripheral innervation; also, no ultrastructural difference was observed among amputated control and sham-control limbs that had intact nerves. This investigation is the first to provide a fine-structure analysis of the effects of denervation and delayed amputation on the earlier stages of tissue regeneration in X. laevis froglets.

scholarly journals Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hamed Nosrati ◽  
Reza Aramideh Khouy ◽  
Ali Nosrati ◽  
Mohammad Khodaei ◽  
Mehdi Banitalebi-Dehkordi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
The Body ◽  
Key Factors ◽  
Potential Applications ◽  
Nanocomposite Scaffolds

AbstractSkin is the body’s first barrier against external pathogens that maintains the homeostasis of the body. Any serious damage to the skin could have an impact on human health and quality of life. Tissue engineering aims to improve the quality of damaged tissue regeneration. One of the most effective treatments for skin tissue regeneration is to improve angiogenesis during the healing period. Over the last decade, there has been an impressive growth of new potential applications for nanobiomaterials in tissue engineering. Various approaches have been developed to improve the rate and quality of the healing process using angiogenic nanomaterials. In this review, we focused on molecular mechanisms and key factors in angiogenesis, the role of nanobiomaterials in angiogenesis, and scaffold-based tissue engineering approaches for accelerated wound healing based on improved angiogenesis.

An ultrastructural study of the effects of wheat germ agglutinin (WGA) on cell cortex organization during the first cleavage of Xenopus laevis eggs. II. Cortical wound healing

1979 ◽  
Vol 37 (1) ◽  
pp. 59-67
Author(s):  
M. Geuskens ◽  
R. Tencer
Keyword(s):  
Wound Healing ◽  
Plasma Membrane ◽  
Electron Microscope ◽  
Xenopus Laevis ◽  
Ultrastructural Study ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Cell Cortex ◽  
Animal Pole ◽  
Annular Zone

Uncleaved fertilized eggs of Xenopus laevis treated with wheat germ agglutinin (WGA) have been pricked at the animal pole both inside and outside the regressed furrow region. The wounded cortex of both regions has been studied with the electron microscope and compared with the same region of wounded, untreated eggs. In all 3 cases, filaments are organized in an annular zone in the damaged cortex. When the surface is pricked outside the regressed furrow of WGA-treated embryos, bundles of microfilaments radiate from the ring and extend in deep folds which form a ‘star’ around the wound at the surface of the embryo. However, when the surface is pricked in the new membrane of the regressed furrow, filaments are intermingled with internalized portions of the plasma membrane. It is suggested that, when the surface is pricked outside the furrow region, more filaments are mobilized to counteract the tangential retraction of the membrane which has acquired more rigidity after WGA binding.

Mapping the dominant wound healing and soft tissue regeneration QTL in MRL × CAST

2005 ◽  
Vol 16 (12) ◽  
pp. 918-924 ◽  
Author(s):  
Hongrun Yu ◽  
Subburaman Mohan ◽  
Godfred L. Masinde ◽  
David J. Baylink
Keyword(s):  
Wound Healing ◽  
Soft Tissue ◽  
Tissue Regeneration ◽  
Soft Tissue Regeneration

scholarly journals Platelet rich plasma in dermatology and cosmetology

2020 ◽  
Vol 6 (2) ◽  
pp. 288
Author(s):  
Shobhit Mohan ◽  
Lalit Mohan ◽  
Renu Sangal ◽  
Neelu Singh
Keyword(s):  
Wound Healing ◽  
Tissue Regeneration ◽  
Blood Product ◽  
Platelet Rich Plasma ◽  
Autologous Blood ◽  
Detailed Knowledge ◽  
Skin Rejuvenation ◽  
Scar Revision ◽  
Current Review

<p class="abstract">Platelet rich plasma (PRP) therapies in medicine has become increasing popular during the last decade. The interest in in the application of PRP in dermatology and cosmetology has increased recently in different applications such as alopecia, skin rejuvenation, wound healing, scar revision, and tissue regeneration. PRP is an autologous blood product obtained from the blood of the patients. The detailed knowledge about PRP should help clinicians better understand this therapy. In this view, the current review was done for a better understanding of what pathologies can be corrected with PRP.</p>

Sign in / Sign up

Export Citation Format

Share Document