Estimation of nuclear polyhedrosis virus produced in field populations of the European pine sawfly, Neodiprion sertifer (Geoff.) (Hymenoptera: Diprionidae)

1983 ◽  
Vol 61 (8) ◽  
pp. 1857-1861 ◽  
Author(s):  
W. J. Kaupp
Keyword(s):  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Direct Result ◽  
Neodiprion Sertifer ◽  
Sawfly Larvae ◽  
Pine Sawfly ◽  
Early Instar ◽  
European Pine Sawfly ◽  
Nuclear Polyhedrosis ◽  
Appreciable Reduction

The quantity of virus measured as the number of polyhedral inclusion bodies (PIBs) produced and liberated at death from two diseased European pine sawfly (Neodiprion sertifer (Geoff.)) populations was studied over a 3-year period in Britain. As high as 2.3 × 1015 PIBs/ha were produced as a resut of a natural epizootic in one of the populations. Subsequent years saw an appreciable reduction in the quantity of the virus produced, a direct result of the reduction in the number of sawfly larvae infesting each plot. Polyhedra persisting over winter in the host's environment were found to alter the nature of subsequent epizootics by causing virus infection to occur at an earlier stage of larval development than previously observed. This increased the percentage contribution of PIBs from the death of early instar larvae to the total amount of virus produced.

Parasitism of the European Pine Sawfly, Neodiprion sertifer (Geoff.) (Hymenoptera: Diprionidae), in Southwestern Ontario

1958 ◽  
Vol 90 (4) ◽  
pp. 223-225 ◽  
Author(s):  
L. R. Finlayson ◽  
Thelma Finlayson
Keyword(s):  
Neodiprion Sertifer ◽  
Experimental Investigations ◽  
Sawfly Larvae ◽  
Pine Sawfly ◽  
European Pine Sawfly ◽  
Small Vial

Cocoons of Neodiprion sertifer (Geoff.) were collected in southwestern Ontario in 1941, 1943, 1946, 1947, and 1949 for experimental investigations at the Belleville laboratory. A total of 8,326 cocoons were collected, mainly within about eight miles northeast and east of Sarnia, Ont., but a few were collected at Strathroy and Brigden, Ont. Each cocoon was incubated separately in a small vial so that each parasite could be associated with the host cocoon from which it emerged. Sawflies emerged from 68 per cent of the cocoons and parasices from 12 per cent; dissection of the remaining cocoons showed 16 per cent contained dead sawfly larvae and pupae, and four per cent dead parasite larvae.

Inclusions in adults of the European pine sawfly, Neodiprion sertifer (Geoff.)

1968 ◽  
Vol 46 (5) ◽  
pp. 959-963 ◽  
Author(s):  
C. R. Sullivan ◽  
D. R. Wallace
Keyword(s):  
Neodiprion Sertifer ◽  
Larval Rearing ◽  
Frequency Of Occurrence ◽  
Rearing Temperature ◽  
Pine Sawfly ◽  
European Pine Sawfly ◽  
Nuclear Polyhedrosis

Hard, dark-red, tumor-like inclusions that are apparently unrelated to nuclear polyhedrosis have been detected in adult Neodiprion sertifer. The frequency of occurrence of these structures is related to larval rearing temperature. They are consistently more common in adults that have undergone prepupal diapause than in adults that have not had a prepupal diapause. Differences in the incidence of the inclusions occur between families, and the bodies are more commonly found in females than in males.

scholarly journals A Nuclear-Polyhedrosis Virus of Spodoptera frugiperda Isolated in Puerto Rico.

1969 ◽  
Vol 63 (2) ◽  
pp. 162-169
Author(s):  
Goro Kuno
Keyword(s):  
Puerto Rico ◽  
Spodoptera Frugiperda ◽  
Inclusion Bodies ◽  
Nuclear Polyhedrosis Virus ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Heliothis Zea ◽  
Fourth Instar ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

A nuclear-polyhedrosis virus was isolated from a larva of Spodoptera frugiperda collected in Puerto Rico. The virus was found to be pathogenic to the larvae of Heliothis zea and of S. frugiperda but nonpathogenic to those of Diatraea saccharalis and of Galleria mellonella. LD50 for the fourth instar larvae of H. zea and S. frugiperda inoculated per os were 1.25 x 103 and 2.7 x 103 polyhedral inclusion bodies, respectively. The tissues infected included hemocytes, fat body, muscle, and epidermis. Furthermore, transovarian transmission of the virus was found in the inoculated individuals of S. frugiperda.

PERSISTENCE AND DISPERSAL OF THE NUCLEAR POLYHEDROSIS VIRUS OF NEODIPRION SERTIFER (GEOFFROY) (HYMENOPTERA: DIPRIONIDAE) IN A VIRUS-FREE LODGEPOLE PINE PLANTATION IN SWEDEN

1988 ◽  
Vol 120 (10) ◽  
pp. 887-892 ◽  
Author(s):  
Einar Olofsson
Keyword(s):  
Nuclear Polyhedrosis Virus ◽  
Lodgepole Pine ◽  
Larval Mortality ◽  
Neodiprion Sertifer ◽  
Experimental Plot ◽  
Pine Plantation ◽  
Larval Population ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis ◽  
High Level

AbstractAn outbreak of Neodiprion sertifer (Geoffroy) was studied in a lodgepole pine plantation. It was the first tree generation on a 60-ha peatland area. The nuclear polyhedrosis virus (NPV) of N. sertifer was not found in the larval population or in the soil. Within a 1.7-ha experimental plot, a 0.35-ha block was treated with NPV and the ensuing epizootic was studied during three successive summers. The treatment caused 50% mortality of fourth- and fifth-instar larvae. The NPV persisted in the treated block and gradually dispersed into the adjacent blocks. After 2 years, larval mortality was 78% in the treated block and 21% at a distance of 110–125 m from it. The larval population remained at a high level and the outbreak expanded from the experimental plot to the entire 60-ha area in the years following the virus treatment, but few virus-diseased colonies were observed outside the experimental plot. Thus, the capability of this NPV to persist and spread was not sufficient to control and contain the sawfly outbreak.

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