The histology of salivary glands in the colubrid snake Sibynomorphus mikanii (Schlegel, 1837)

1983 ◽  
Vol 61 (4) ◽  
pp. 936-941 ◽  
Author(s):  
M. G. D. Contrera ◽  
R. A. Lopes ◽  
J. R. V. Costa ◽  
S. O. Petenusci ◽  
J. S. Lima-Verde
Keyword(s):  
Salivary Glands ◽  
Nuclear Volume ◽  
Mucous Cells ◽  
Tubule Cells ◽  
Acid Mucosubstances

A study of the morphology of the salivary glands of the colubrid snake Sibynomorphus mikanii showed the following: (i) the acini of supralabial, infralabial, and premaxillary glands are formed by mucous cells, the tubules of lateral and median posterior sublingual glands are formed by mucoserous cells, and the Duvemoy's gland by seromucous cells; and (ii) mucous cells produce neutral and acid mucosubstances, mucoserous cells secrete neutral and acid mucosubstances and protein, and seromucous cells have neutral mucosubstance and protein secretions. The nuclear volume of acinar and tubule cells were evaluated morphometrically.

scholarly journals Electron Microscopic Immunogold Localization of Salivary Mucins MG1 and MG2 in Human Submandibular and Sublingual Glands

2003 ◽  
Vol 51 (1) ◽  
pp. 69-79 ◽  
Author(s):  
Marco Piludu ◽  
Sean A. Rayment ◽  
Bing Liu ◽  
Gwynneth D. Offner ◽  
Frank G. Oppenheim ◽  
...  
Keyword(s):  
Salivary Glands ◽  
Expression Patterns ◽  
Cell Types ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Dense Cores ◽  
Duct Cells ◽  
Rabbit Igg ◽  
Lowicryl K4m

The human salivary mucins MG1 and MG2 are well characterized biochemically and functionally. However, there is disagreement regarding their cellular and glandular sources. The aim of this study was to define the localization and distribution of these two mucins in human salivary glands using a postembedding immunogold labeling method. Normal salivary glands obtained at surgery were fixed in 3% paraformaldehyde-0.1% glutaraldehyde and embedded in Lowicryl K4M or LR Gold resin. Thin sections were labeled with rabbit antibodies to MG1 or to an N-terminal synthetic peptide of MG2, followed by gold-labeled goat anti-rabbit IgG. The granules of all mucous cells of the submandibular and sublingual glands were intensely reactive with anti-MG1. No reaction was detected in serous cells. With anti-MG2, the granules of both mucous and serous cells showed reactivity. The labeling was variable in both cell types, with mucous cells exhibiting a stronger reaction in some glands and serous cells in others. In serous granules, the electron-lucent regions were more reactive than the dense cores. Intercalated duct cells near the acini displayed both MG1 and MG2 reactivity in their apical granules. In addition, the basal and lateral membranes of intercalated duct cells were labeled with anti-MG2. These results confirm those of earlier studies on MG1 localization in mucous cells and suggest that MG2 is produced by both mucous and serous cells. They also indicate differences in protein expression patterns among salivary serous cells.

scholarly journals Effect of temperature on dry mass of polytene nuclei in Drosophila

1979 ◽  
Vol 38 (1) ◽  
pp. 405-416
Author(s):  
I.J. Hartmann-Goldstein ◽  
D.J. Goldstein
Keyword(s):  
Salivary Glands ◽  
Dry Mass ◽  
Nuclear Volume ◽  
Interference Microscopy ◽  
Effect Of Temperature ◽  
Percentage Loss ◽  
Mass Group ◽  
The Mean ◽  
Polytene Nuclei ◽  
Aqueous Detergent

Nuclei were isolated by an aqueous detergent method from Drosophila prepupal salivary glands, and measured by integrating interference microscopy. There was a highly significant correlation between nuclear volume and dry mass. Dry masses fell into 2, 3 or 4 distinct groups corresponding to polytene replication classes; the mean of a given dry mass group was between 8 and 30% less than twice that of the group below, indicating that the ratio of DNA:dry mass increases during polytenic growth. The proportion of nuclei in the higher mass groups, the mean dry mass of nuclei within a given mass group, and the percentage loss of nuclear dry mass in the first hour after isolation were all higher when animals were reared at 15 degrees instead of 25 degrees C. Nuclear dry mass in prepupae was affected by the temperature during both the embryonic and larval periods, and also to some extent by the nutrition and degree of crowding of the cultures.

20-OH-ecdysone swells nuclear volume by alkalinization in salivary glands of Drosophila melanogaster

1993 ◽  
Vol 274 (1) ◽  
pp. 145-151 ◽  
Author(s):  
Stefan W�nsch ◽  
Stefan Schneider ◽  
Albrecht Schwab ◽  
Hans Oberleithner
Keyword(s):  
Drosophila Melanogaster ◽  
Salivary Glands ◽  
Nuclear Volume

scholarly journals Mucus formation in goblet cells

1933 ◽  
Vol 113 (784) ◽  
pp. 459-463 ◽  
Keyword(s):  
Golgi Apparatus ◽  
Salivary Glands ◽  
Secretory Granules ◽  
Goblet Cells ◽  
Neutral Red ◽  
Basal Region ◽  
Mucous Cells ◽  
Cell Type ◽  
Golgi Network ◽  
Golgi Zone

The formation of mucus in goblet cells and its relation to the Golgi apparatus has been studied by various workers. Nassanow (1923) showed clearly that the mucin granules in the goblet cells of Triton originated in the Golgi apparatus, and so brought secretion in these cells into line with his theory of the bound secretion. More recently Clara (1926) has shown in the goblet cells of birds that the mucin first appears in the region next to the nucleus, between it and the gland lumen. Florey (1932, a, b ) has considered this more extensively in two recent papers, and for a number of mammals has shown that the mucin granules of goblet cells first form in the meshes of the Golgi network. In epithelial cells of the mouse vagina, undergoing conversion into mucous cells, he has found that the same process occurs. In a recent investigation of secretory formation in the salivary glands and pancreas it was found by the present author that in every cell type examined the young secretory granules first appeared in the basal region of the cell in relation to the mitochondria. Subsequent emigration occurred into the Golgi zone, where they underwent conversion into mature secretory granules. In the mucous cells of the salivary glands it was shown that these newly formed granules might be stained intravitam by Janus green or neutral red, and that in fixed preparations they stained selectively with acid fuchsin as described by Noll (1902), In the light of this work it appeared probable that while mucin formation might occur in the Golgi zone of the goblet cells as described by these authors, the origin of the granules might lie in the basal region of the cell.

scholarly journals Structure and biosynthesis of human salivary mucins.

2000 ◽  
Vol 47 (4) ◽  
pp. 1067-1079 ◽  
Author(s):  
A Zalewska ◽  
K Zwierz ◽  
K Zółkowski ◽  
A Gindzieński
Keyword(s):  
Molecular Mass ◽  
Salivary Glands ◽  
Disulfide Bonds ◽  
Tandem Repeats ◽  
Mucous Cells ◽  
Central Domain

Human salivary glands secrete two types of mucins: oligomeric mucin (MG1) with molecular mass above 1 MDa and monomeric mucin (MG2) with molecular mass of 200-250 kDa. Monomers of MG1 and MG2 contain heavily O-glycosylated tandem repeats located at the central domain of the molecules. MG1 monomers are linked by disulfide bonds located at sparsely glycosylated N- and C-end. MG1 are synthesized by mucous cells and MG2 by the serous cells of human salivary glands.

The foregut glands of vermivorous cone shells

1971 ◽  
Vol 19 (4) ◽  
pp. 313 ◽  
Author(s):  
H Marsh
Keyword(s):  
Salivary Gland ◽  
Salivary Glands ◽  
Protease Activity ◽  
Proteolytic Enzymes ◽  
Gland Secretion ◽  
Salivary Gland Secretion ◽  
Acid Mucosubstances

Salivary, snout, and secondary salivary glands are recorded in the foregut region of Conus Javidus, C. lividus, C. litteratus, C. miles, C. vexillum, and C. virgo. C. imperialis has a salivary gland only. The histology and histochemistry of these glands in C. Javidus and C, lividus is investigated. The salivary gland secretion consists of granules composed of polysaccharide and protein. The snout gland secretes sulphated acid mucosubstances. The secondary salivary gland secretion contains polysaccharide and protein. Protease activity was detected histochemically in the salivary gland, indicating that a function of this gland in Conus is the secretion of proteolytic enzymes into the short arm of the radular sac. The functions of the snout gland, which is peculiar to some species of Conus, and of the secondary salivary gland, which occurs sporadically throughout the Stenoglossidae, are not immediately apparent.

The Histochemistry of the labial Salivary Glands of the Spiny-Tailed Lizard Uromastyx microlepis (Blandford)*

1987 ◽  
Vol 8 (1) ◽  
pp. 59-67 ◽  
Author(s):  
Bashir M. Jarrar ◽  
Noory T. Taib
Keyword(s):  
Alkaline Phosphatase ◽  
Carbonic Anhydrase ◽  
Salivary Glands ◽  
Succinic Dehydrogenase ◽  
Mucous Cells ◽  
Secrete Protein ◽  
Protein Radicals ◽  
Mixed Nature

AbstractThe labial salivary glands of the spiny-tailed lizard, Uromastyx microlepis (Blandford) were used in morphometric, histological and histochemical investigations. Both supra and infralabial glands are of the compound tubulo-acinar type and are of a mixed nature containing both mucous and mucoserous cells. The mucous cells secrete and elaborate neutral mucosubstances, sialomucins and hyaluronidase resistant sulfomucins, whereas the mucoserous cells secrete protein radicals as well. The histoenzymological tests used have detected alkaline phosphatase, succinic dehydrogenase and carbonic anhydrase in these glands.

scholarly journals Subcellular localization of blood group substances ABH in human salivary glands.

1990 ◽  
Vol 38 (8) ◽  
pp. 1165-1172 ◽  
Author(s):  
M Cossu ◽  
A Riva ◽  
M S Lantini
Keyword(s):  
Monoclonal Antibodies ◽  
Subcellular Localization ◽  
Salivary Glands ◽  
Blood Group ◽  
Secretory Granules ◽  
Type A ◽  
Relative Distribution ◽  
Mucous Cells ◽  
Blood Types ◽  
Blood Group Substances

We investigated the subcellular localization of ABH antigens in human submandibular, sublingual, and buccal glands by applying a post-embedding immunogold method using monoclonal antibodies specific for A, B, and H antigens. In most glands the immunoreactivity was usually restricted to mucous cells, in which only secretory granules and sometimes Golgi cisternae were specifically labeled. A and B antigens were demonstrated only in the glands of type A, B, and AB subjects, while H antigen was visualized in glands from individuals of all blood types. Moreover, differences were observed in the relative distribution of ABH antigens, depending on the type of gland.

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