Plasma concentrations of testosterone and luteinizing hormone in rutting reindeer bulls (Rangifer tarandrus)

1980 ◽  
Vol 58 (11) ◽  
pp. 2081-2083 ◽  
Author(s):  
K.-A. Stokkan ◽  
K. Hove ◽  
W. R. Carr
Keyword(s):  
Luteinizing Hormone ◽  
Testosterone Level ◽  
Plasma Concentrations ◽  
Plasma Testosterone ◽  
Plasma Samples ◽  
Diurnal Pattern ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
The Mean

Concentrations of plasma testosterone and luteinizing hormone (LH) were measured in peripheral plasma from semidomestic, rutting reindeer bulls. Although the concentrations of plasma testosterone were high and showed large variations, those of LH were low and only a few episodic bursts could be detected in hourly samples taken throughout a 48-h period. The mean testosterone concentration in three bulls differed significantly and ranked the animals according to their position in a fighting hierarchy. The mean concentrations of LH did not differ significantly. Plasma samples from one reindeer bull sampled every 20 min for periods of 3 h indicated that an increment in LH concentration preceded a peak in testosterone. No diurnal pattern in testosterone concentrations could be detected, but testosterone peaks seemed to appear about every 3–4 h. The present study thus demonstrates that a series of plasma samples throughout the day is necessary to determine a true "testosterone level" in the reindeer bull.

TESTOSTERONE AND OESTRADIOL IN DOGS WITH TESTICULAR TUMOURS

1974 ◽  
Vol 77 (2) ◽  
pp. 408-416 ◽  
Author(s):  
F. Comhaire ◽  
D. Mattheeuws ◽  
A. Vermeulen
Keyword(s):  
Plasma Concentration ◽  
Sertoli Cell ◽  
Sex Steroids ◽  
Venous Blood ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Normal Mass ◽  
The Mean ◽  
Sertoli Cell Tumours

ABSTRACT The mean peripheral plasma concentration of oestradiol was found to be increased in 3 dogs with Sertoli cell tumours and in 3 dogs with seminomas, whereas the plasma testosterone showed no difference as compared to a group of dogs without testicular neoplasia. In two thirds of the cases the concentration of oestradiol in the spermatic venous blood draining the neoplastic testes was clearly higher than in the normal dogs. The testosterone concentration in the spermatic venous blood from the tumour bearing testes was lower than in the spermatic venous blood of the contralateral partner testes in the same dogs, though not different from the spermatic venous concentration in the control dogs. There was no correlation between the presence or absence of signs of feminization and the peripheral or spermatic venous concentration of sex steroids. It is concluded that not only Sertoli cell tumours, but also seminomas can secrete increased amounts of oestrogens. This is possibly due to the presence of a larger than normal mass of tissue capable of converting testosterone, or its precursors, to oestradiol.

STIMULATORY EFFECT OF PROSTAGLANDIN F2α ON THE PLASMA CONCENTRATION OF TESTOSTERONE IN BULLS

1975 ◽  
Vol 66 (3) ◽  
pp. 329-338 ◽  
Author(s):  
N. B. HAYNES ◽  
H. D. HAFS ◽  
R. J. WATERS ◽  
J. G. MANNS ◽  
A. RILEY
Keyword(s):  
Plasma Concentration ◽  
Intramuscular Injection ◽  
Prostaglandin F2α ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Prostaglandin F ◽  
The Mean

SUMMARY The concentrations of peripheral plasma testosterone were measured by radioimmunoassay in samples collected from five bulls, each given i.m. injections of 0, 5, 15, 30 and 60 mg prostaglandin F2α (PGF2α). Synchronized peaks in testosterone concentration occurred with maximum values 1–2 h after treatment. These increases of testosterone persisted significantly longer than those observed to occur as natural episodic peaks during two 24 h periods in the same bulls. The mean peak testosterone concentration after PGF2α injection was related to the dose of PGF2α, values after 60 and 30 mg doses being significantly greater than after 15 mg. The response produced by a 5 mg dose was not significant. The results indicate that intramuscular injection of PGF2α acutely stimulates synthesis and release of testosterone in bulls.

Studies of the oestrous cycle, oestrus and pregnancy in the koala (Phascolarctos cinereus)

Reproduction ◽  
2000 ◽  
pp. 49-57 ◽  
Author(s):  
SD Johnston ◽  
MR McGowan ◽  
P O'Callaghan ◽  
R Cox ◽  
V Nicolson
Keyword(s):  
Luteal Phase ◽  
Post Partum ◽  
Plasma Concentrations ◽  
External Genitalia ◽  
Oestrous Cycle ◽  
Phascolarctos Cinereus ◽  
Pregnant Females ◽  
Peripheral Plasma ◽  
The Mean ◽  
High Concentrations

As an integral part of the development of an artificial insemination programme in the captive koala, female reproductive physiology and behaviour were studied. The oestrous cycle in non-mated and mated koalas was characterized by means of behavioural oestrus, morphology of external genitalia and changes in the peripheral plasma concentrations of oestradiol and progestogen. The mean (+/- SEM) duration of the non-mated oestrous cycle and duration of oestrus in 12 koalas was 32.9 +/- 1.1 (n = 22) and 10.3 +/- 0.9 (n = 24) days, respectively. Although the commencement of oestrous behaviour was associated with increasing or high concentrations of oestradiol, there were no consistent changes in the morphology or appearance of the clitoris, pericloacal region, pouch or mammary teats that could be used to characterize the non-mated cycle. As progestogen concentrations remained at basal values throughout the interoestrous period, non-mated cycles were considered non-luteal and presumed anovulatory. After mating of the 12 koalas, six females gave birth with a mean (+/- SEM) gestation of 34.8 +/- 0.3 days, whereas the remaining six non-parturient females returned to oestrus 49.5 +/- 1. 0 days later. After mating, oestrous behaviour ceased and the progestogen profile showed a significant increase in both pregnant and non-parturient females, indicating that a luteal phase had been induced by the physical act of mating. Progestogen concentrations throughout the luteal phase of the pregnant females were significantly higher than those of non-parturient females. Parturition was associated with a decreasing concentration of progestogen, which was increased above that of basal concentrations until 7 days post partum.

Peripheral plasma testosterone concentration and sexual behavior in young prenatally stressed boars

1979 ◽  
Vol 12 (5) ◽  
pp. 289-305 ◽  
Author(s):  
H.G. Kattesh ◽  
E.T. Kornegay ◽  
F.C. Gwazdauskas ◽  
J.W. Knight ◽  
H.R. Thomas
Keyword(s):  
Sexual Behavior ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Peripheral Plasma ◽  
Prenatally Stressed

Plasma steriod hormone concentrations in relation to reproductive cycle of the sweep Scorpis lineolatus (Kyphosidae) caught from the wild

1992 ◽  
Vol 43 (4) ◽  
pp. 753 ◽  
Author(s):  
M Deual ◽  
NW Pankhurst
Keyword(s):  
Steroid Hormones ◽  
Reproductive Cycle ◽  
Gonadosomatic Index ◽  
Plasma Concentrations ◽  
Plasma Testosterone ◽  
Hepatosomatic Index ◽  
Spawning Period ◽  
Testosterone Concentration ◽  
Low Concentrations ◽  
Advanced Stages

Changes in gonadosomatic index (GSI), hepatosomatic index (HSI), oocyte development and plasma levels of steroid hormones were studied during the reproductive cycle of the sweep Scorpis lineolatus. GSI values for both sexes were maximal in March and April. During this period the size distribution of oocytes showed a predominance of advanced stages of vitellogenesis, whereas males were completing spermatogenesis. However, staging data suggest that spawning may not begin until April. HSI was maximal 2 months prior to the beginning of gametogenesis in both sexes. Plasma concentrations of oestradiol-17β in females increased rapidly from February, reached a peak (2.3 ng mL-1) in association with the end of vitellogenesis in March, and returned to low concentrations in May and June. Plasma concentrations of 17α,20α-dihydroxy-4-pregnen-3-one (17,20βP) also peaked in March in females, but 17,20βP was generally not detectable in males. Plasma testosterone concentration peaked along with other steroids in March in females but showed no significant change in males. Plasma ll-ketotestosterone (1 1KT) concentration was maximal in males before spawning (February), but was not elevated during the spawning period itself. Changes in oestradiol-17β and testosterone in association with vitellogenensis in females are consistent with those reported for other teleosts, whereas changes in 17,20βP, testosterone and 11-ketotestosterone in males are different from those previously reported.

PLASMA TESTOSTERONE AND SHELL QUALITY

1989 ◽  
Vol 69 (4) ◽  
pp. 1107-1109
Author(s):  
S. LEESON ◽  
C. D. BENNETT ◽  
L. J. CASTON ◽  
C. E. ANDERSON-LANGMUIR ◽  
R. J. ETCHES
Keyword(s):  
Key Words ◽  
Testosterone Level ◽  
Plasma Testosterone ◽  
Production Cycle ◽  
Testosterone Concentration ◽  
Shell Quality ◽  
Testosterone Levels ◽  
Commercial Strain ◽  
Eggshell Quality ◽  
Key Words Testosterone

Plasma testosterone levels were measured in commercial strain Leghorn pullets from 16 to 80 wk of age. Testosterone level was high just prior to first egg, but subsequently declined and stayed at a constant level through the production cycle. There was no relationship between testosterone concentration and eggshell quality (P > 0.05). Key words: Testosterone, shell quality

PLASMA TESTOSTERONE IN WOMEN IN LATE PREGNANCY AND AFTER DELIVERY

1968 ◽  
Vol 42 (3) ◽  
pp. 477-481 ◽  
Author(s):  
K. DEMISCH ◽  
J. K. GRANT ◽  
W. BLACK
Keyword(s):  
Protein Binding ◽  
Late Pregnancy ◽  
Plasma Testosterone ◽  
Testosterone Concentration ◽  
Healthy Women ◽  
The Mean

SUMMARY The concentration of testosterone was measured in the plasma of 16 healthy women in late pregnancy and in 14 of these after delivery. The mean testosterone concentration was found to be elevated in late pregnancy, although the range overlapped with the normal. There was a prompt return to normal concentrations after delivery. The significance of the results and of inactivation of testosterone by protein binding are discussed.

DETERMINATION OF DEHYDROEPIANDROSTERONE AND DEHYDROEPIANDROSTERONE SULPHATE IN HUMAN PLASMA USING ELECTRON CAPTURE DETECTION OF 4-ANDROSTENE-3,6,17-TRIONE AFTER GAS-LIQUID CHROMATOGRAPHY

1972 ◽  
Vol 53 (3) ◽  
pp. 461-474 ◽  
Author(s):  
F. H. de JONG ◽  
H. J. van der MOLEN
Keyword(s):  
Liquid Chromatography ◽  
Electron Capture ◽  
Plasma Concentrations ◽  
Mean Value ◽  
Electron Capture Detection ◽  
Gas Liquid Chromatography ◽  
Mean Values ◽  
Peripheral Plasma ◽  
Competitive Protein Binding ◽  
The Mean

SUMMARY A method for the measurement of dehydroepiandrosterone (DHA) and of its sulphate (DHAS) in human peripheral plasma is described and evaluated. After isolation of DHA from the sample the steroid is oxidized to 4-androstene-3,6,17-trione, which is measured with an electron capture detector after gas—liquid chromatography. It is possible to detect 100 pg 4-androstene-3,6,17-trione. The smallest amount of DHA per sample that can be distinguished from zero is approximately 4 ng, when recovery (27·9 ± 8·8%) and method blank (0·23 ± 0·38 ng) are taken into account. The oxidation to 4-ene-3,6-diones is specific for steroidal 5-en-3-ols. Specificity for DHA is ensured by several chromatographic steps. Repeated estimation of 10 ng DHA gave a mean value of 9·6 ± 1·45 (s.d.) ng (n = 35). Mean concentrations and their standard deviations for DHA and DHAS in peripheral plasma from 18 individuals were 0·50 ± 0·25 and 78 ± 40 μg/100 ml, respectively, at 08.30 h and 0·32 ± 0·17 and 84 ± 34 μg/100 ml, respectively, at 17.00 h of the same day. Levels of plasma cortisol in the same plasma samples estimated with a competitive protein-binding method were 16·7 ± 1·8 and 11·9 ± 3·8 μg/100 ml, respectively. No significant differences between the sexes were observed by any of the three assays. The mean values of the plasma concentrations of cortisol and DHA in the morning were significantly higher than those in the evening (P < 0·001 and P < 0·005, respectively). In contrast, the mean value of the plasma levels of DHAS in the morning was significantly lower than that in the evening (P < 0·025).

Endocrine correlates of territoriality, breeding stage, and body molt in free-living willow ptarmigan of both sexes

1990 ◽  
Vol 68 (10) ◽  
pp. 2130-2134 ◽  
Author(s):  
Susan J. Hannon ◽  
John C. Wingfield
Keyword(s):  
Luteinizing Hormone ◽  
Aggressive Behaviour ◽  
Follicle Stimulating Hormone ◽  
Plasma Concentrations ◽  
Egg Laying ◽  
Plasma Testosterone ◽  
Willow Ptarmigan ◽  
Free Living ◽  
Brood Rearing ◽  
Egg Formation

Concentrations of plasma testosterone, estradiol, corticosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH) were measured by radioimmunoassay in wild willow ptarmigan (Lagopus lagopus) of both sexes over the breeding season. In males, plasma concentrations of testosterone and LH increased once they became territorial, and this was paralleled by increases in comb size and initiation of molt into courtship plumage. A decline of testosterone and circulating LH levels during late territoriality and egg laying coincided with molt into summer plumage. FSH titers did not vary significantly through the season, but there was a trend for higher levels during the territorial period. Plasma concentrations of corticosterone were highest during pre- and late territoriality and brood rearing. In females, testosterone levels were about 13 times lower than in males and did not vary significantly, whereas LH was high from pre- to mid-territoriality and again in the brood season. Elevated LH levels may be correlated with aggressive behaviour in females. FSH and estradiol concentrations were highest during the period of egg formation. Corticosterone was correlated with chase time in both sexes.

EFFECT OF AN ANABOLIC STEROID (METANDIENON) ON PLASMA LH, FSH, AND TESTOSTERONE AND ON THE RESPONSE TO INTRAVENOUS ADMINISTRATION OF LRH

1976 ◽  
Vol 83 (4) ◽  
pp. 856-864 ◽  
Author(s):  
Pentti Holma ◽  
Herman Adlercreutz
Keyword(s):  
Luteinizing Hormone ◽  
Intravenous Administration ◽  
Anabolic Steroid ◽  
Plasma Levels ◽  
Oral Intake ◽  
Plasma Testosterone ◽  
Before And After ◽  
The Mean ◽  
Pre Treatment ◽  
Lh Secretion

ABSTRACT Plasma levels of testosterone, luteinizing hormone (LH) and follicle-stimulating hormone (FSH) as well as the response of LH and FSH to the intravenous administration of 100 μg of luteinizing hormone releasing hormone (LRH) were measured in 16 well-trained athletes (mean age 30 years) before and after 2 months of daily oral intake of 15 mg of metandienon, an anabolic steroid (Anabolin®, 17α-methyl-17β-hydroxy-1,4-androstadien-3-one, Medica, Finland). All athletes continued to train regularly, just as they had done for several years. During administration of metandienon the mean plasma testosterone level fell 69%, from 29.4 ± 11.6 nmol/l to 9.1 ± 7.5 nmol/l. The mean plasma levels of LH and FSH also fell significantly (P < 0.001 and P < 0.01, respectively), both about 50%. Because LH and FSH levels were low after administration of the steroid the maximum stimulation values after LRH administration were also lower than pre-treatment values although the mean increments did not differ significantly before and after administration of the anabolic steroid. However, after treatment, the FSH response curve had a biphasic pattern in most subjects, with peaks at 10 to 20 and 50 to 60 min after the iv injection of LRH. Administration of LRH after the treatment period had no effect on FSH secretion in two subjects and no effect on LH secretion in one. Our results show that administration of an anabolic steroid causes a pronounced lowering of plasma levels of testosterone, LH and FSH but causes no gross alteration in the response of LH secretion to stimulation by LRH. The reason for the biphasic response pattern of FSH to LRH administration in most subjects is not known.

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