Arachidonic acid requirement for two species of Culiseta reared on synthetic diet

1980 ◽  
Vol 58 (10) ◽  
pp. 1845-1850 ◽  
Author(s):  
R. H. Dadd ◽  
W. G. Friend ◽  
J. E. Kleinjan
Keyword(s):  
Arachidonic Acid ◽  
Fatty Acid ◽  
Amino Acid ◽  
Synthetic Diet ◽  
Essential Nutrient ◽  
Nutritional Effects ◽  
Culiseta Inornata ◽  
Aseptic Conditions ◽  
Amino Acid Concentrations ◽  
Culiseta Incidens

Culiseta incidens and Culiseta inornata larvae were successfully reared under aseptic conditions on a completely defined chemical medium. Arachidonic acid is an essential nutrient for both species. Larvae of C. incidens would develop and pupate in the absence of this fatty acid but emergence was impaired and none of the resulting adults could fly. None of the C. inornata larvae reared on diets lacking arachidonic acid became adult. Many of the C. inornata adults that emerged from diets containing this fatty acid could fly. Amino acid concentrations in the diet of 0.9% were superior to 1.4% for C. inornata. Asparagine was shown to be an essential nutrient for C. incidens; its nutritional effects were not tested on C. inornata.

scholarly journals KARAKTERISTIK ASAM AMINO DAN ASAM LEMAK BEKASAM KERANG BULU (Anadara antiquate) DI DESA BENAN KABUPATEN LINGGA

Marinade ◽  
2020 ◽  
Vol 3 (02) ◽  
pp. 159-167
Author(s):  
M. Desra Hari Putra ◽  
R. Marwita Sari Putri ◽  
Yulia Oktavia ◽  
Aidil Fadli Ilhamdy
Keyword(s):  
Amino Acids ◽  
Fatty Acids ◽  
Arachidonic Acid ◽  
Fatty Acid ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Linolenic Acid ◽  
Saturated Fatty Acid ◽  
Lauric Acid ◽  
Acid Yield

Formation of feather shells (Anadara antiquata) which has been carried out the characteristics of amino acids and fatty acids obtained the highest amino acid yield is Arginine 10293.28, and the lowest histidine is 136.91. The highest saturated fatty acid is palmitic which is 1.20 and the lowest lauric acid is 0.09, the highest monounsaturated fatty acid is glutamic acid 17257.96, and the lowest is alanine 312.56, the highest polyunsaturated fatty acid is linoleic 0.58, linolenic acid 0.58, and the lowest arachidonic acid 0.29.

In Vitro Incorporation and Metabolism of Icosapentaenoic and Docosahexaenoic Acids in Human Platelets - Effect on Aggregation

1986 ◽  
Vol 56 (01) ◽  
pp. 057-062 ◽  
Author(s):  
Martine Croset ◽  
M Lagarde
Keyword(s):  
Arachidonic Acid ◽  
Fatty Acid ◽  
Platelet Aggregation ◽  
Fatty Acid Distribution ◽  
Thromboxane A2 ◽  
Human Platelets ◽  
Aggregation Response ◽  
Docosahexaenoic Acids ◽  
Membrane Level

SummaryWashed human platelets were pre-loaded with icosapentaenoic acid (EPA), docosahexaenoic acid (DHA) or EPA + DHA and tested for their aggregation response in comparison with control platelets. In fatty acid-rich platelets, an inhibition of the aggregation could be observed when induced by thrombin, collagen or U-46619. The strongest inhibition was observed with DHA-rich platelets and it was reduced when DHA was incorporated in the presence of EPA.Study of fatty acid distribution in cell lipids after loading showed that around 90% of EPA or DHA taken up was acylated into phospholipids and a very small amount (less than 2%) remained in their free and hydroxylated forms. DHA was more efficiently acylated into phosphatidylethanolamine (PE) than into phosphatidylinositol (PI) in contrast to what observed with EPA, and both acids were preferentially incorporated into phosphatidylcholine (PC). EPA inhibited total incorporation of DHA and increased its relative acylation into PE at the expense of PC. In contrast, DHA did not affect the acylation of EPA. Upon stimulation with, thrombin, EPA was liberated from phospholipids and oxygenated (as judged by the formation of its monohydroxy derivative) whereas DHA was much less metabolized, although consistently transferred into PE.It is concluded that EPA and DHA might affect platelet aggregation via different mechanisms when pre-loaded in phospholipids. Whereas EPA is known to alter thromboxane A2 metabolism from endogenous arachidonic acid, by competing with it, DHA might act directly at the membrane level for inhibiting aggregation.

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