On the validity of the genus Musculium (Bivalvia: Sphaeriidae): electrophoretic evidence

1980 ◽  
Vol 58 (9) ◽  
pp. 1703-1707 ◽  
Author(s):  
Daniel J. Hornbach ◽  
M. J. McLeod ◽  
S. I. Guttman
Keyword(s):  
Gel Electrophoresis ◽  
Habitat Characteristics ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Patterns ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Phenotypic Convergence

Starch gel electrophoresis was used to examine the generic separation of the sphaeriid clams Musculium and Sphaerium. Twenty-five loci from 13 enzyme systems were resolved. Major differences in electrophoretic patterns were observed, and these appear to support the generic separation of Musculium and Sphaerium. Sphaerium occidentale, considered to be intermediate in form between Musculium and Sphaerium, is more closely related (electrophoretically) to other spieces of Sphaerium than to Musculium. The phenotypic convergence of S. occidentale with Musculium and its genotypic divergence from other species of Sphaerium can be related to habitat characteristics of these forms.

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

1988 ◽  
Vol 66 (3) ◽  
pp. 588-594 ◽  
Author(s):  
Hong Zhu ◽  
Kenneth O. Higginbotham ◽  
Bruce P. Dancik ◽  
Stan Navratil
Keyword(s):  
Genetic Variability ◽  
Host Selection ◽  
Gel Electrophoresis ◽  
Genetic Similarity ◽  
Ectomycorrhizal Fungus ◽  
Habitat Isolation ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Forest Regions ◽  
Starch Gel

Mycelial extracts of 43 isolates of Suillus tomentosus (Kauffm.) Singer, Snell & Dick collected from four boreal forest regions in Alberta were subjected to starch gel electrophoresis. A total of 21 bands was resolved from eight different enzyme systems presumably representing 13 loci. Six loci were polymorphic among these isolates. Cluster and principal components analyses demonstrated that intraspecific genetic variability of this fungus existed among and within forest regions. Polymorphic loci of acid phosphatase and alkaline phosphatase exhibited the greatest genetic similarity among the isolates within forest regions. Habitat isolation and host selection could be the major sources of genetic variation among forest regions.

Isozyme Variation in Broom Snakeweed (Gutierrezia sarothrae)

Weed Science ◽  
1995 ◽  
Vol 43 (1) ◽  
pp. 156-162 ◽  
Author(s):  
Yanglin Hou ◽  
Tracy M. Sterling
Keyword(s):  
Cluster Analysis ◽  
New Mexico ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Isozyme Analysis ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Morphological Differences ◽  
Gutierrezia Sarothrae

Broom snakeweed, a perennial rangeland shrub, is highly variable morphologically and can grow under a broad range of environmental conditions. In this study, isozyme analysis using starch gel electrophoresis was used to quantify genetic variability within and among New Mexico populations of broom snakeweed. Eight separate populations of broom snakeweed and one population of threadleaf snakeweed as a comparison were investigated. of the 10 enzyme systems examined, 16 loci were identified in eight populations and two species. Eleven loci were monomorphic in eight populations and two species and five loci were polymorphic in at least one population or species. Genetic variability was large in broom and threadleaf snakeweed populations as determined by isozyme analysis. Genetic variability among broom snakeweed populations was greater than that within populations for the five polymorphic loci. Cluster analysis of genetic distance and identity for the eight populations and two species characterized two major groups. Within broom snakeweed, cluster analysis characterized five groups. The two species shared most common alleles. The genetic variation identified in this research may account for the morphological differences and broad geographical distribution of broom snakeweed.

scholarly journals Erythrocyte glutathione S-transferase. Electrophoretic identification of two enzyme forms

1983 ◽  
Vol 215 (1) ◽  
pp. 213-216 ◽  
Author(s):  
R C Strange ◽  
P H Hirrell ◽  
G A Kitley ◽  
D A Hopkinson ◽  
W Cotton
Keyword(s):  
Enzyme Activity ◽  
Individual Differences ◽  
Gel Electrophoresis ◽  
Human Erythrocytes ◽  
Starch Gel Electrophoresis ◽  
Glutathione S Transferase ◽  
Electrophoretic Patterns ◽  
Starch Gel

Starch-gel electrophoresis was used to demonstrate two forms of glutathione S-transferase in human erythrocytes. Whereas considerable inter-individual differences in enzyme activity and electrophoretic patterns were detected, intra-individual differences were small.

scholarly journals Genetic variation in the triploids of Japanese Fasciola species, and relationships with other species in the genus

1994 ◽  
Vol 68 (3) ◽  
pp. 181-186 ◽  
Author(s):  
T. Agatsuma ◽  
K. Terasaki ◽  
L. Yang ◽  
D. Blair
Keyword(s):  
United States Of America ◽  
Gel Electrophoresis ◽  
Laboratory Strain ◽  
Morphological Type ◽  
The United States ◽  
Starch Gel Electrophoresis ◽  
Isozyme Patterns ◽  
Electrophoretic Patterns ◽  
Starch Gel ◽  
Liver Flukes

AbstractTwelve enzymes (encoded by 14 loci) in liver flukes of Fasciola species originating from Japan (parthenogenetic triploids), Korea (parthenogenetic diploids), the United States of America (USA) and Australia (all sexual diploids) were analysed using starch gel electrophoresis. Variation in electrophoretic patterns between samples was detected at five enzyme loci (Ak, Got, Gpi, 6-Pgd and Pgm-2). Japanese worms (31, of which six were established as uniparental laboratory strains), which reproduce by parthenogenesis, exhibited three different isozyme patterns. This indicates that triploidy has arisen more than once in Japanese flukes. Japanese Fasciola sp. can be separated into three types on morphological grounds. For the six laboratory strains of Japanese worms, the parental morphological type was known. Each of the three isozyme patterns observed was restricted to one morphological type. Most alleles detected in the Japanese triploids were also found in diploid worms from the other countries: the only alleles not represented elsewhere were four at the Got locus and two at the Pgm locus. Flukes from a laboratory strain derived from a single Korean diploid worm resembled the Japanese worms in genotype more closely than did American (seven uniparental laboratory strains) or Australian (30 worms) specimens. Worms from the last two countries were closely related.

scholarly journals Inheritance of Phosphoglucoisomerase in Fountain Grass

1995 ◽  
Vol 120 (3) ◽  
pp. 543-547
Author(s):  
M. Hockenberry Meyer ◽  
Donald B. White
Keyword(s):  
Gel Electrophoresis ◽  
Growth Habit ◽  
Mendelian Inheritance ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Segregation Ratios ◽  
Starch Gel ◽  
Pennisetum Alopecuroides ◽  
Slow Band ◽  
Growth Habits

Starch gel electrophoresis was used to screen 10 enzyme systems for variation in fountain grass, Pennisetum alopecuroides (L.) Spreng. plants exhibiting four different growth habits: dwarf(d), mound(m), prostrate(p), and upright (u). Only phosphoglucoisomerase (PGI; E.C. 5.3.1.9) was found to be polymorphic at one locus, PGI-2, and was expressed as two alleles, which appeared to be associated with growth habit. The dwarf form expressed one slow band (SS), the mound and prostrate forms exhibited one fast band (FF), and the upright form carried triple bands indicating a heterodimer (FS). Hybrids between FF and SS parents were detected as triple bands (FS). Three generations of progeny resulting from 16 crosses and selfs of these growth habits all followed the expected segregation ratios for typical Mendelian inheritance of this isozyme.

scholarly journals Isozymes for Cultivar Identification in Kiwifruit

HortScience ◽  
1991 ◽  
Vol 26 (7) ◽  
pp. 899-902 ◽  
Author(s):  
R. Messina ◽  
R. Testolin ◽  
M. Morgante
Keyword(s):  
New Zealand ◽  
Genetic Markers ◽  
Gel Electrophoresis ◽  
Cultivar Identification ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Discriminating Power ◽  
Enzyme Systems ◽  
Starch Gel ◽  
Simultaneous Comparison

The usefulness of isozyme banding patterns as genetic markers in kiwifruit [Actinidia deliciosa (A. Chev.) C.F. Liang et A.R. Ferguson] was investigated using starch gel electrophoresis. Fifty-four entries putatively belonging to seven female and two male kiwifruit cultivars were examined for 13 enzyme systems (AAT, ACO, GDH, G6PDH, IDH, MDH, ME, MNR, NDH, 6PGD, PGI, PGM, and SKDH). Four enzyme systems, ACO, MDH, NDH, and SKDH, showed identical banding patterns in all clones surveyed. Of the remaining enzymes, AAT, PGI, and PGM had the best discriminating power. Six enzyme systems (GDH, G6PDH, IDH, ME, MNR, and 6PGD), though showing polymorphic banding patterns, were poorly resolved. All the New Zealand cultivars were uniquely identified by the simultaneous comparison of the AAT, PGI, and PGM zymograms. Some enzyme systems were also polymorphic among plants within the same cultivar, thus proving the heterogeneity of kiwifruit material introduced into Europe in the early 1970s.

ELECTROPHORETIC ANALYSIS OF DIVERSITY WITHIN CUPRESSUS SEMPERVIRENS L. GROWING IN ISRAEL

1997 ◽  
Vol 45 (1) ◽  
pp. 1-8
Author(s):  
Gabriel Schiller ◽  
Leonid Korol
Keyword(s):  
Gel Electrophoresis ◽  
Gene Diversity ◽  
Electrophoretic Analysis ◽  
Starch Gel Electrophoresis ◽  
Cupressus Sempervirens ◽  
Single Tree ◽  
Enzyme Systems ◽  
High Gene ◽  
Starch Gel ◽  
Viable Seeds

Isoenzyme variants withinCupressus sempervirensL. planted in Israel have been identified using starch gel electrophoresis of enzymes extracted from the megagametophytic and perisperm seed tissue. Single-tree cone collection from 493 trees growing in 27 populations at holy sites and in plantations planted at the end of the 19th and the beginning of the 20th century in the country was done in 1986–1987. In 1994 viable seeds were available from only 267; of them, 140 trees were phenotypically identified as var.pyramidalisand 67 as being of var.horizontalis; the other 60 trees were not identified phenotypically. The results gained show that in the plantedC. sempervirensL. under investigation 11 (47.8%) out of the 23 gene loci in 13 enzyme systems analyzed were polymorphic; they contain a relatively high gene diversity of 0.479. The genetic distance between the two varieties planted in Israel is 0.007. Differences in allele frequencies between the two varieties occurred only in 5 loci, viz., IDH2PGI2, MDH3, PGM1, and ACO1.

CHARACTERISTIC ELECTROPHORETIC PATTERNS OF SERUM PROTEINS OF SEVERAL SPECIES OF SNAKES OF IRAN

1965 ◽  
Vol 43 (4) ◽  
pp. 459-461 ◽  
Author(s):  
M. Latifi ◽  
K. D. Shamloo ◽  
A. Amin
Keyword(s):  
Gel Electrophoresis ◽  
Serum Proteins ◽  
Starch Gel Electrophoresis ◽  
Gel Method ◽  
Electrophoretic Patterns ◽  
Starch Gel

Paper and starch-gel electrophoresis of serum proteins of several species and subspecies of poisonous and nonpoisonous snakes of Iran have been investigated. The patterns obtained, especially by means of the starch-gel method, are characteristic for each species. Electrophoretic patterns of samples of serum from different individuals of the same species were very similar.

scholarly journals Growth and isoenzyme comparison of five isolates of Venturia inaequalis

2005 ◽  
Vol 71 (2) ◽  
pp. 65-71 ◽  
Author(s):  
E. Roig ◽  
P. Neumann ◽  
J.-P. Simon
Keyword(s):  
Carbonic Anhydrase ◽  
Gel Electrophoresis ◽  
Venturia Inaequalis ◽  
Starch Gel Electrophoresis ◽  
Enzyme Systems ◽  
Race 1 ◽  
Starch Gel ◽  
Specific Proteins ◽  
Race 2 ◽  
Isoenzyme Patterns

Observation of the mycelial aspect of five isolates representing the fîve races of Venturia inaequalis indicates that it is possible to differentiate these isolates by their growth pattern and the isolate of race 3 appears to be the most easily distinguishable. Fifteen enzyme systems and non-specific proteins have been analyzed by polyacrylamide and starch gel electrophoresis. Thirteen of the isoenzymes systems and non-specific proteins showed no variation among the five isolates. Esterases isoenzyme patterns allow separation of the isolates since only one isoenzyme is common to all isolates and two others are shared by isolates of race 1 and 5. One carbonic anhydrase isoenzyme was observed to be specific to the isolate of race 2.

A COMPARISON OF HISTONES FROM CHICKEN TISSUES BY ZONE ELECTROPHORESIS IN STARCH GEL

1961 ◽  
Vol 39 (3) ◽  
pp. 485-491 ◽  
Author(s):  
J. M. Neelin ◽  
G. C. Butler
Keyword(s):  
Cell Differentiation ◽  
Ionic Strength ◽  
Gel Electrophoresis ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Electrophoretic Patterns ◽  
Zone Electrophoresis ◽  
Starch Gel ◽  
Chicken Erythrocytes ◽  
Characteristic Zone

Histories were extracted at pH 1.7 from washed nuclei of chicken erythrocytes, spleen, liver, and testis and compared by starch-gel electrophoresis at pH 5.0, ionic strength 0.020. Spleen and liver histories displayed the most complex electrophoretic patterns with 18 zones each and differed only in relative proportions of certain zones. Erythrocyte histone contained a characteristic zone while lacking a group present in spleen and liver histones. Testis histone with only seven zones differed markedly from the other three. These results were consistent with chromatograms of erythrocyte, spleen, and liver histones on sodium IRC-50. The suggested correlation of tissue-specific histones with cell differentiation is discussed.

Sign in / Sign up

Export Citation Format

Share Document