The estimation of natality in a fruit-infesting insect (Diptera: Tephritidae)
Extraction of eggs of Dacus tryoni from fruit was aided by first identifying oviposition sites with a water-soluble dye and then digesting the fruit tissue around the oviposition site with a mixture of enzymes consisting of equal parts of 1% pectinase and 1% cellulase. The numbers of eggs laid per week were estimated by a formula that incorporated the numbers of hatched and unhatched eggs present on one occasion each week, the proportion of fertile eggs in each variety of fruit, and the development time of eggs.Many more eggs were laid in an orchard in New South Wales, Australia, in a wet year (1966–1967) than in a dry year (1965–1966). This was due in part to a larger population of adults that migrated into the orchard in the wet year and to the fact that fruit remained on the trees longer. There was evidence that the rate of oviposition in the wet season fell off as fruit became scarcer towards the end of the season. Aggressive behaviour of females on fruit and movement of gravid females away from the orchard are possible explanations.