Immunodiffusion comparisons of the serum albumins of marine and land iguanas from different islands in the Galapagos Archipelago

1977 ◽  
Vol 55 (9) ◽  
pp. 1389-1392 ◽  
Author(s):  
Paul J. Higgins
Keyword(s):  
Rabbit Antiserum ◽  
Double Diffusion ◽  
Exchange Chromatography ◽  
Santa Cruz Island ◽  
Santa Cruz ◽  
Serum Albumins ◽  
Galapagos Archipelago ◽  
Amblyrhynchus Cristatus ◽  
Marine Iguana ◽  
Diffusion Assay

Serum albumin of the Santa Cruz Island Galapagos marine iguana, Amblyrhynchus cristatus, was isolated by ion-exchange chromatography of an acid-precipitable alcohol-soluble fraction of whole serum. Antigenic differences could not be resolved among the albumins of several morphologically diverse marine iguana populations in agar double-diffusion assay using rabbit antiserum to the albumin of A. cristatus of Santa Cruz Island. The antigenic composition of the serum albumins of Iguana and Conolophus were similar, relative to Amblyrhynchus, although Conolophus albumin possessed determinants cross-reactive with marine iguana albumin which were not found in Iguana.

scholarly journals The Red-fin Goby, Evorthodus minutus Meek & Hildebrand, 1928: a new record from Santa Cruz Island, Galapagos Archipelago

Check List ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 1-5
Author(s):  
Omar Valencia-Méndez ◽  
Dave Catania ◽  
Andrés López-Pérez
Keyword(s):  
Brackish Water ◽  
New Record ◽  
Central American ◽  
Range Extension ◽  
Eastern Pacific ◽  
Santa Cruz Island ◽  
Santa Cruz ◽  
Galapagos Archipelago ◽  
Brackish Water Species ◽  
Water Species

The Red-fin Goby, Evorthodus minutus Meek & Hildebrand, 1928, is a coastal brackish species which is commonly distributed from Sinaloa, Mexico to Guayaquil, Ecuador and particularly abundant in mangroves of Central American eastern Pacific. We report a new record of E. minutus collected from the Santa Cruz Island, part of the Galapagos Archipelago. This new record represents a range extension and is allows for a relevant discussion about colonization pathways in the equatorial eastern Pacific of a brackish-water species.

scholarly journals Mitigation Options for Future Water Scarcity: A Case Study in Santa Cruz Island (Galapagos Archipelago)

Water ◽  
2017 ◽  
Vol 9 (8) ◽  
pp. 597 ◽  
Author(s):  
Maria Reyes ◽  
Nemanja Trifunović ◽  
Saroj Sharma ◽  
Kourosh Behzadian ◽  
Zoran Kapelan ◽  
...  
Keyword(s):  
Water Scarcity ◽  
Santa Cruz Island ◽  
Santa Cruz ◽  
Mitigation Options ◽  
Galapagos Archipelago

New highly polymorphic microsatellite loci for the Galápagos marine iguana, Amblyrhynchus cristatus

2012 ◽  
Vol 33 (3-4) ◽  
pp. 533-536 ◽  
Author(s):  
Amy MacLeod ◽  
Volker Koch ◽  
Carolina García-Parra ◽  
Fritz Trillmich ◽  
Sebastian Steinfartz
Keyword(s):  
Microsatellite Loci ◽  
Galapagos Archipelago ◽  
Amblyrhynchus Cristatus ◽  
Dinucleotide Motif ◽  
Hardy Weinberg Equilibrium ◽  
Marine Iguanas ◽  
Successful Amplification ◽  
Marine Iguana ◽  
Unique Species ◽  
Significant Linkage

We describe the development and characterisation of six new dinucleotide motif microsatellite loci for populations of marine iguanas (Amblyrhynchus cristatus), endemic to the Galápagos archipelago. Primers were based on microsatellite-bearing sequences and initially developed using universally labelled primers. When analysed across 5 populations (representing 150 individuals), new loci displayed, on average, high levels of genetic diversity (range: 2-13 alleles, mean: 5.73) and values of heterozygosity (range: 0.0-0.906, mean: 0.605). No consistent deviations from Hardy-Weinberg equilibrium or significant linkage disequilibrium were observed, and all loci were shown to be free of common microsatellite errors. Utilising the 13 previously available microsatellite loci for this species, we describe here four multiplex combinations for the successful amplification of 19 microsatellite loci for marine iguanas. This powerful set of highly polymorphic markers will allow researchers to explore future questions regarding the ecology, evolution, and conservation of this unique species.

scholarly journals Galápagos Rail Laterallus spilonotus population change associated with habitat invasion by the Red-barked Quinine Tree Cinchona pubescens

2010 ◽  
Vol 21 (2) ◽  
pp. 221-227 ◽  
Author(s):  
W. GREGORY SHRIVER ◽  
JAMES P. GIBBS ◽  
HARA W. WOLTZ ◽  
NICOLE P. SCHWARZ ◽  
MARGARET A. PEPPER
Keyword(s):  
Population Change ◽  
Oceanic Islands ◽  
Native Vegetation ◽  
Santa Cruz Island ◽  
Santa Cruz ◽  
Vegetation Communities ◽  
Galapagos Archipelago ◽  
Management Actions ◽  
Cinchona Pubescens ◽  
Habitat Invasion

SummarySpecies on oceanic islands are more likely to be endemic as well as more extinction-prone than those on continents. The Galápagos Rail Laterallus spilonotus, endemic to the Galápagos Archipelago, is presently known to occur on just four of its seven previously occupied islands and is facing multiple threats to its persistence. In this study, we compared the rail’s occurrence and abundance at 193 survey points between 2000 and 2007 on Santa Cruz Island and examined the influence of an invasion of the habitat of the species by the exotic Red-barked Quinine Tree Cinchona pubescens. We detected a 13% reduction in rail occurrence and a 31% reduction in abundance between 2000 and 2007. Rail abundance declined more in low elevation areas (< 719 m) and outside of Cinchona pubescens removal areas but not in areas where Cinchona pubescens was removed. Insofar as Galápagos Rails responded positively to management actions that promote and maintain native vegetation, we conclude that restoration projects that restore native vegetation communities benefit this apparently declining endemic bird for which more focused conservation attention is warranted.

Misconceptions about the ability of researchers to relocate the holotype of the Galapagos pink land iguana through the use of a passive integrated transponder

Zootaxa ◽  
2010 ◽  
Vol 2694 (1) ◽  
pp. 57 ◽  
Author(s):  
MICHAEL G. FRICK
Keyword(s):  
Threatened Species ◽  
Marine Species ◽  
Lizard Species ◽  
International Union ◽  
Terrestrial Environment ◽  
Passive Integrated Transponder ◽  
Galapagos Archipelago ◽  
Amblyrhynchus Cristatus ◽  
The Status ◽  
Marine Iguana

Isla Isabela is the largest island in the Galapagos archipelago and hosts three endemic iguanid lizard species (Tzika et al. 2008, Gentile & Snell 2009): Amblyrhynchus cristatus (Bell, 1825), Conolophus subcristatus (Gray, 1831) and Conolophus marthae Gentile & Snell, 2009. The first iguanid listed exists as a marine species and the latter two are restricted to the terrestrial environment. It should be noted, however, that all three species can hybridize and produce viable offspring (Tzika et al. 2008). The International Union for Conservation of Nature (IUCN) lists the marine iguana A. cristatus as a “threatened” species and the land iguana species C. subcristatus as “vulnerable”. Currently there are no studies that assess the status of the iguana C. marthae, so its status is given as “not evaluated” by the IUCN.

Immunological Evidence for Prothrombin in Human Platelets

1986 ◽  
Vol 55 (02) ◽  
pp. 268-270
Author(s):  
R J Alexander
Keyword(s):  
Electrophoretic Mobility ◽  
Platelet Activation ◽  
Double Diffusion ◽  
Human Platelets ◽  
Secreted Proteins ◽  
Platelet Surface ◽  
Activated Platelets ◽  
Similar Protein ◽  
Diffusion Assay ◽  
Supernatant Solution

SummaryAn attempt was made to isolate from plasma the platelet surface substrate for thrombin, glycoprotein V (GPV), because a GPV antigen was reported to be present in plasma (3). Plasma fractionation based on procedures for purification of GPV from platelets revealed a thrombin-sensitive protein with appropriate electrophoretic mobility. The protein was purified; an antiserum against it i) reacted with detergent-solubilized platelet proteins or secreted proteins in a double diffusion assay, ii) adsorbed a protein from the supernatant solution of activated platelets, and iii) inhibited thrombin-induced platelet activation, but the antiserum did not adsorb labeled GPV. The purified protein was immunochemically related to prothrombin rather than to GPV. Other antibodies against prothrombin were also able to adsorb a protein from platelets. It is concluded that 1) plasma does not contain appreciable amounts of GPV, and 2) platelets contain prothrombin or an immunochemically similar protein.

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