Ultrastructure of sex pheromone gland cells in Lobesia botrana Den &Schiff. (Lepidoptera: Olethreutidae)

1977 ◽  
Vol 55 (4) ◽  
pp. 672-680 ◽  
Author(s):  
B. Lalanne-Cassou ◽  
Jean Percy ◽  
J. A. MacDonald
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Sex Pheromone ◽  
Lipid Droplets ◽  
Lobesia Botrana ◽  
Apical Plasma Membrane ◽  
Pheromone Gland ◽  
Gland Cells ◽  
Sex Pheromone Gland ◽  
Tubular Endoplasmic Reticulum

In newly emerged and 48-h-old female adults of Lobesia botrana the sex pheromone gland cells are columnar. Organelles characteristic of lipid-producing cells are very prominent. Smooth tubular endoplasmic reticulum is extensively developed but few lipid droplets are observed. Numerous microbodies are present and are located, along with the Golgi complexes, primarily in the vicinity of the nucleus. The apical plasma membrane is folded. The cuticle overlying the gland cells differs from unmodified cuticle in the number and location of epicuticular filaments. They originate near the bases of the furrows between the apical folds.

Development and ultrastructure of sex-pheromone gland cells in females of the cabbage looper moth, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae)

1979 ◽  
Vol 57 (1) ◽  
pp. 220-236 ◽  
Author(s):  
Jean Percy
Keyword(s):  
Sex Pheromone ◽  
Trichoplusia Ni ◽  
Cabbage Looper ◽  
Apical Plasma Membrane ◽  
Tubular Structures ◽  
Gland Cells ◽  
Cabbage Looper Moth ◽  
Sex Pheromone Gland ◽  
Lepidoptera Noctuidae ◽  
Tubular Endoplasmic Reticulum

The sex-pheromone-producing gland in female Trichoplusia ni (Hübner) is a modified intersegmental membrane and the gland cells are ductless. Lipid spheres are located throughout gland cells and vary both in number and size relative to the age of the female. Most of the lipid is surrounded by oval to elongate distensions of smooth tubular endoplasmic reticulum which contain the enzyme catalase and are thus microperoxisomes. Lipid spheres evert the apical plasma membrane between microvilli, move away from the gland cells, and are stored in the cuticle as discrete lipid deposits. These deposits, in turn, move to the surface of the gland by tubular structures that differ from epicuticular filaments.

Haemocytes associated with basement membrane of sex pheromone gland of Trichoplusia ni (Lepidoptera: Noctuidae). Ultrastructural observations

1978 ◽  
Vol 56 (2) ◽  
pp. 238-245 ◽  
Author(s):  
Jean Percy
Keyword(s):  
Sex Pheromone ◽  
Basement Membrane ◽  
Trichoplusia Ni ◽  
Amorphous Layer ◽  
Epidermal Cells ◽  
Pheromone Gland ◽  
Gland Cells ◽  
Sex Pheromone Gland ◽  
Layer 2 ◽  
Lepidoptera Noctuidae

In female Trichoplusia ni, granular haemocytes are observed near the basement membrane of developing sex pheromone gland cells while plasmatocytes are observed near the basement membrane of unmodified epidermal cells. The basement membrane underlying gland cells is clearly different from that of other epidermal cells. There is a thin amorphous layer (layer 1) which is also present beneath unmodified cells, and a second layer (layer 2) apposing the haemocytes. Layer 2 is distinctly banded which results from tubules similar in dimensions and structural appearance to those observed within granules of the granular haemocytes. The observations indicate that the granules participate in the formation of layer 2 by emptying their contents into the haemocoel next to layer 1.

scholarly journals Specific Heterodimer Formation Is a Prerequisite for Uroplakins to Exit from the Endoplasmic Reticulum

2002 ◽  
Vol 13 (12) ◽  
pp. 4221-4230 ◽  
Author(s):  
Liyu Tu ◽  
Tung-Tien Sun ◽  
Gert Kreibich
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Posttranslational Modifications ◽  
Cell Layer ◽  
Building Blocks ◽  
Apical Plasma Membrane ◽  
Membrane Bound ◽  
Umbrella Cells ◽  
Vesicular Compartment ◽  
Lower Urinary

Much of the lower urinary tract, including the bladder, is lined by a stratified urothelium forming a highly differentiated, superficial umbrella cell layer. The apical plasma membrane as well as abundant cytoplasmic fusiform vesicles of the umbrella cells is covered by two-dimensional crystals that are formed by four membrane proteins named uroplakins (UPs) Ia, Ib, II, and III. UPs are synthesized on membrane-bound polysomes, and after several co- and posttranslational modifications they assemble into planar crystals in a post-Golgi vesicular compartment. Distension of the bladder may cause fusiform vesicles to fuse with the apical plasma membrane. We have investigated the early stages of uroplakin assembly by expressing the four uroplakins in 293T cells. Transfection experiments showed that, when expressed individually, only UPIb can exit from the endoplasmic reticulum (ER) and move to the plasma membrane, whereas UPII and UPIII reach the plasma membrane only when they form heterodimeric complexes with UPIa and UPIb, respectively. Heterodimer formation in the ER was confirmed by pulse-chase experiment followed by coimmunoprecipitation. Our results indicate that the initial building blocks for the assembly of crystalline uroplakin plaques are heterodimeric uroplakin complexes that form in the ER.

The morphology of the sex pheromone gland inDrosophila grimshawi

1979 ◽  
Vol 161 (2) ◽  
pp. 177-183 ◽  
Author(s):  
R. J. Hodosh ◽  
E. M. Keough ◽  
J. M. Ringo
Keyword(s):  
Sex Pheromone ◽  
Pheromone Gland ◽  
Sex Pheromone Gland

Notes: Identification of the Sex Pheromone of Eggplant Borer Leucinodes orbonalis Guenèe (Lepidoptera: Pyralidae)

1987 ◽  
Vol 42 (11-12) ◽  
pp. 1347-1348 ◽  
Author(s):  
Zhu Pingchou ◽  
Kong Fanlei ◽  
Yu Shengdi ◽  
Yu Yongqing ◽  
Jin Shuping ◽  
...  
Keyword(s):  
Sex Pheromone ◽  
Pheromone Gland ◽  
Leucinodes Orbonalis ◽  
Gland Extract ◽  
Sex Pheromone Gland ◽  
Lepidoptera Pyralidae

(E)11-Hexadecenyl acetate was identified from sex pheromone gland extract of female eggplant borer. The acetate synthesized in the laboratory showed high attractant activity in the field.

scholarly journals Apolipoprotein L1-Specific Antibodies Detect Endogenous APOL1 inside the Endoplasmic Reticulum and on the Plasma Membrane of Podocytes

2020 ◽  
Vol 31 (9) ◽  
pp. 2044-2064 ◽  
Author(s):  
Suzie J. Scales ◽  
Nidhi Gupta ◽  
Ann M. De Mazière ◽  
George Posthuma ◽  
Cecilia P. Chiu ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Cell Surface ◽  
High Frequency ◽  
Lipid Droplets ◽  
Human Kidney ◽  
Cytoplasmic Face ◽  
Large Panel ◽  
Surface Localization ◽  
Apolipoprotein L1

BackgroundAPOL1 is found in human kidney podocytes and endothelia. Variants G1 and G2 of the APOL1 gene account for the high frequency of nondiabetic CKD among African Americans. Proposed mechanisms of kidney podocyte cytotoxicity resulting from APOL1 variant overexpression implicate different subcellular compartments. It is unclear where endogenous podocyte APOL1 resides, because previous immunolocalization studies utilized overexpressed protein or commercially available antibodies that crossreact with APOL2. This study describes and distinguishes the locations of both APOLs.MethodsImmunohistochemistry, confocal and immunoelectron microscopy, and podocyte fractionation localized endogenous and transfected APOL1 using a large panel of novel APOL1-specific mouse and rabbit monoclonal antibodies.ResultsBoth endogenous podocyte and transfected APOL1 isoforms vA and vB1 (and a little of isoform vC) localize to the luminal face of the endoplasmic reticulum (ER) and to the cell surface, but not to mitochondria, endosomes, or lipid droplets. In contrast, APOL2, isoform vB3, and most vC of APOL1 localize to the cytoplasmic face of the ER and are consequently absent from the cell surface. APOL1 knockout podocytes do not stain for APOL1, attesting to the APOL1-specificity of the antibodies. Stable re-transfection of knockout podocytes with inducible APOL1-G0, -G1, and -G2 showed no differences in localization among variants.ConclusionsAPOL1 is found in the ER and plasma membrane, consistent with either the ER stress or surface cation channel models of APOL1-mediated cytotoxicity. The surface localization of APOL1 variants potentially opens new therapeutic targeting avenues.

Morphology of female sex pheromone gland in the horse chestnut leafminer Cameraria ohridella (Lep., Gracillariidae)

2003 ◽  
Vol 127 (3) ◽  
pp. 121-126 ◽  
Author(s):  
G. Raspotnig ◽  
R. Schicho ◽  
E. Stabentheiner ◽  
C. Magnes ◽  
M. Stelzl
Keyword(s):  
Sex Pheromone ◽  
Horse Chestnut ◽  
Pheromone Gland ◽  
Female Sex Pheromone ◽  
Female Sex ◽  
Sex Pheromone Gland
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