Action du jeûne et de Bacillus thuringiensis Berliner sur les protéines plasmatiques de Choristoneura fumiferana (Clem.) (Lepidoptera : Tortricidae)

1977 ◽  
Vol 55 (3) ◽  
pp. 543-552 ◽  
Author(s):  
F. Paré ◽  
L. Huot ◽  
J. M. Perron ◽  
M. Cloutier
Keyword(s):  
Bacillus Thuringiensis ◽  
Total Protein ◽  
Fast Moving ◽  
Protein Concentration ◽  
Choristoneura Fumiferana ◽  
Hemolymph Protein ◽  
Slow Moving

Total protein contents of the hemolymph of Choristoneura fumiferana larvae, prepupae, and pupae were studied. These were electrophoretically separated into 14 bands, classified as slow moving and fast moving proteins. During the sixth instar, hemolymph protein contents increased eight to nine times in control larvae mainly owing to slow moving proteins. Protein concentration of slow moving proteins were less pronounced in larvae that were starved and treated with Bacillus thuringiensis, the treated larvae being more affected than the starved larvae. Larvae treated on day 1 of the 6th instar were more affected than those treated on day 4.

Recherche d'une Méthode d'Utilisation du Bacillus thuringiensis pour Combattre les Insectes Forestiers

1964 ◽  
Vol 96 (1-2) ◽  
pp. 152-152
Author(s):  
W. A. Smirnoff
Keyword(s):  
Bacillus Thuringiensis ◽  
Choristoneura Fumiferana

Parmi les espèces de bactéries comprises dans le groupe “cereus”, il en existe une très pathogène pour certaines espèces d'insectes, c'est le Bacillus thuringiensis Berliner. Ce bacille produit des spores et des cristaux toxiques dans la cellule et excrète diverses substances toxiques dans son milieu de culture. L'action du B. thuringiensis varie avec les espèces d'insectes; chez certaines il provoque une septicémie typique, chez d'autres il cause une intoxication, alors que quelques espèces sont réfractaires à ses attaques. Depuis quelques années notre laboratoire envisage la possibilité d'utiliser le B. thuringiensis et d'autres espèces proche parentes dans la lutte à certains insectes forestiers fort nuisibles tels que la tordeuse des bourgeons de l'épinette (Choristoneura fumiferana (Clem.)), l'un des plus graves ravageurs des forês de conifères dans l'est de l'Amérique du Nord. En laboratoire avec une suspension contenant 200 gr. du pathogène par gallon d'eau, toutes les chenilles en expérience sont mortes de septicémie typique dans l'espace de 8 jours. Lors d'expériences conduites dans un peuplement hébergeant une forte population de I'insecte et dans des conditions climatiques optimales, 84 pour 100 de la population larvaire fut détruite en dedans de 24 jours après l'application d'une suspension contenant 80 gr. du pathogène par gallon d'eau. Ces premiers résultats nous incitérent à rechercher une lignée du B. thuringiensis plus pathogène pour le C. fumiferana surtout aux basses températures, et capable de résister à l'action bactéricide et bactériostatique de certaines substances produites par le feuillage du sapin et de l'épinette.

Determination of Total Protein Concentration in Solution Using Gold Electrode Modified with Silver Nanoparticles

2014 ◽  
Vol 27 (1) ◽  
pp. 253-257 ◽  
Author(s):  
Patrick Marcel Seumo Tchekwagep ◽  
Charles Péguy Nanseu-Njiki ◽  
Emmanuel Ngameni ◽  
Ravi Danielsson ◽  
Thomas Arnebrant ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Total Protein ◽  
Gold Electrode ◽  
Protein Concentration ◽  
Total Protein Concentration

LONG TERM STUDY OF THE EFFECTIVENESS OF AERIAL APPLICATION OF BACILLUS THURINGIENSIS – ACEPHATE COMBINATIONS AGAINST THE SPRUCE BUDWORM, CHORISTONEURA FUMIFERANA (LEPIDOPTERA: TORTRICIDAE)

1977 ◽  
Vol 109 (9) ◽  
pp. 1239-1248 ◽  
Author(s):  
O. N. Morris
Keyword(s):  
Bacillus Thuringiensis ◽  
Choristoneura Fumiferana ◽  
Larval Mortality ◽  
Abies Balsamea ◽  
Spruce Budworm ◽  
White Spruce ◽  
Balsam Fir ◽  
Population Densities ◽  
Long Term Study

AbstractBacillus thuringiensis (Dipel® 36B) mixed with a sublethal concentration of acephate (Orthene®) (O, S-dimethyl acetylphosphoramidothioate), an organophosphorous insecticide, was applied at 2.35–14 l./ha to white spruce (Picea glauca) and balsam fir (Abies balsamea) trees infested with spruce budworm, Choristoneura fumiferana (Clem.). The treatment rate was 20 Billion International Units of B. thuringiensis (B.t.) activity with or without 42 g of active ingredient of acephate/ha.The ground deposit of the standard Dipel wettable powder formulation was 12% of emitted volume compared with 21–32% for the Dipel 36B flowable. The viability of B.t. spores was drastically reduced after 1 day of weathering but a high level of biological activity by the spore–crystal complex persisted for up to 20 days post-spray due probably to crystal activity.The addition of about 10% of the recommended operational rate of acephate to the B.t. suspension increased larval mortality by 34% when applied at 4.7 l./ha. Reductions in budworm populations were 97–99% in B.t. + acephate plots and 86–90% in B.t. alone plots.Plots with moderate budworm densities of up to 27 larvae/100 buds on white spruce and 36/100 on balsam fir were satisfactorily protected from excessive defoliation in the year of spray by B.t. with or without acephate. Plots with higher population densities were not satisfactorily protected based on the branch sample examination but aerial color photographs indicated good protection to the top third of the trees. Population declines were greater and defoliation and oviposition were lower in the treated plots than in the untreated checks 1 year later without further treatment. Two years later the larval population densities in all plots were low but the density was twice as high in the untreated check as in the treated plots, indicating long term suppression by the treatments. Defoliation was negligible in all plots.The treatments had no deleterious effect on spruce budworm parasitism. The data indicate that the integrated approach using Bacillus thuringiensis – chemical pesticide combinations is a viable alternative to the use of chemical pesticides alone in spruce budworm control. Large scale testing is now warranted.

Mineral balance in skim-milk and milk retentate: effect of physicochemical characteristics of the aqueous phase

1981 ◽  
Vol 48 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Gerard Brule ◽  
Jacques Fauquant
Keyword(s):  
Aqueous Phase ◽  
Total Protein ◽  
Protein Concentration ◽  
Skim Milk ◽  
Physicochemical Characteristics ◽  
Mineral Balance ◽  
Citrate Ions

SummaryThe effect of physicochemical characteristics (pH, temperature, composition) of the aqueous phase on the mineral balance in milk and milk retentate has been studied. The ratio of colloidal Ca to total protein decreased with pH, but at any given pH the higher the protein concentration, the higher was the ratio of colloidal Ca to total protein. The solubilization of Ca during cooling and the decrease in soluble Ca during heating were approximately the same in retentates and in milk. Among the components of the aqueous phase, soluble Ca and citrate ions were related to the amount of colloidal Ca.

Albumin fraction and measurement of total protein concentration

1993 ◽  
Vol 264 (5) ◽  
pp. H1723-H1726 ◽  
Author(s):  
B. T. Peterson ◽  
R. W. Tate
Keyword(s):  
Total Protein ◽  
Protein Concentration ◽  
Gamma Globulin ◽  
Colorimetric Assay ◽  
Full Range ◽  
Computational Method ◽  
Total Protein Concentration ◽  
Albumin Fraction ◽  
Standard Curve ◽  
Protein Assays

The standard curve of a typical colorimetric assay for total protein is often nonlinear and dependent on the albumin fraction of the protein standard. We developed a simple mathematical transformation to make the standard curve linear and a computational method to correct for differences in albumin concentrations among the samples. This method uses data from total protein assays on two sets of standards (albumin and gamma globulin) and provides accurate measures of total protein over the full range of albumin fractions. Comparison of this two-standard method with the a method that uses only albumin as a standard shows that this method prevents physiologically significant overestimations in total protein concentration and calculated protein osmotic pressure differences in the lungs.

scholarly journals Analisis Industri Retail Nasional

Humaniora ◽  
2011 ◽  
Vol 2 (2) ◽  
pp. 1309
Author(s):  
Handy Martinus
Keyword(s):  
Fast Moving ◽  
Retail Industry ◽  
Global Crisis ◽  
Revenue Growth ◽  
Research Material ◽  
Slow Moving ◽  
Document Review

Fast moving in retail industry is the latest trading industry in trading chain. The study is to identify the condition of retail industry in Indonesia. This uses normative juridical approach. The research material is gathered by literature or document review. The analysis for modern retail industry is conducted through qualitative, thus explaining law material gathered from library being selected, arranging systematically, and finally getting conclusion figured to answer problems related to the rules for monopoly and dysfunction business competition in retail industry. The study result showed that modern market, which so far having a good performance, will have challenges. One biggest challenge is the potential for slow-moving revenue growth as the effects of slow-moving economic caused by global crisis.  

scholarly journals Metode FSN Analysis Dan Implementasinya Pada Desain Sistem Informasi Pergudangan

2021 ◽  
Vol 15 (1) ◽  
pp. 60
Author(s):  
Amri Yanuar ◽  
Marwanto Rahmatuloh ◽  
Ilman Mutaqien
Keyword(s):  
Fast Moving ◽  
Order Picking ◽  
Bottle Neck ◽  
Slow Moving

Operasional gudang memiliki berbagai aktivitas inti yang saling berkaitan satu dengan yang lainnya, seperti aktivitas receiving, storage, dan picking. Aktivitas inti ini atau yang biasa disebut inbound dan outbound harus dikerjakan dalam waktu yang singkat dan meminimalisir tingkat kesalahan agar tidak mengganggu keberlangsungan alur masuk dan keluarnya barang di gudang. Beberapa hal yang dapat mengganggu aliran barang di gudang di antaranya adalah terjadinya bottle neck di area receiving, waktu proses pencarian barang yang lama, dan 70% waktu proses order picking di bawah waktu standar. Permasalahan-permasalahan ini terjadi salah satunya dikarenakan pengelolaan barang di gudang masih manual atau belum menggunakan sistem informasi. Tujuan dari penelitian ini adalah merancang sistem informasi untuk membantu operasional gudang guna meminimalisir kesalahan-kesalahan yang dapat mengganggu aliran masuka dan keluarnya barang dari gudang, dengan menggunakan pendekatan FSN Analysis. Hasil dari pengolahan data menggunakan FSN Analysis ini, dapat membantu dalam mengetahui produk-produk yang masuk kedalam kategori fast moving, slow moving, dan non-moving.

scholarly journals Role of fibronectin under conditions of doxorubicin action

2015 ◽  
Vol 6 (1) ◽  
pp. 17-22
Author(s):  
A. I. Shevtsova ◽  
G. A. Ushakova
Keyword(s):  
Blood Plasma ◽  
Total Protein ◽  
Protein Concentration ◽  
Male Rats ◽  
Antitumor Action ◽  
Total Protein Concentration ◽  
Monospecific Antibodies ◽  
Anthracycline Chemotherapy ◽  
In Blood Plasma

There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So, the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induced cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature.The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiving saline i/p; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks;4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin application once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in index mass heart in 38% of animals compared to control animals; decrease in total protein concentration by 8% (Р < 0,05) and increase of the level of fibronectin by 67% (P < 0,001) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% (Р < 0,05) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r=0,80) and heart extract (r=0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sensitive reaction of fibronectin to development of metabolic disorders under doxorubicin influence. 

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