Actinlike filaments in the neurosecretory cells of Helisoma duryi (Gastropoda, Pulmonata)

1976 ◽  
Vol 54 (11) ◽  
pp. 2001-2010 ◽  
Author(s):  
A. S. M. Saleuddin ◽  
Gwyneth M. Jones
Keyword(s):  
Neurosecretory Cells ◽  
Neurosecretory Granule ◽  
Heavy Meromyosin ◽  
Cerebral Ganglia ◽  
Helisoma Duryi

The mediodorsal neurosecretory cells in the cerebral ganglia of Helisoma duryi send axons into the median lip nerve, where they terminate to form a probable neurohaemal area. After glycerination these cells are shown to contain a branching network of 5- to 6-nm diameter filaments, which are abundant throughout the axon and axon ending but less prominent in the perikaryon. The filaments react with heavy meromyosin subfraction SI, increasing in thickness and forming arrowhead-shaped complexes; the reaction is reversed by adenosine 5′-triphosphate (ATP). This reaction indicates that the filaments contain actin, and it is suggested that actin–myosin interactions could be responsible for neurosecretory granule transport along the axon.

Polysaccharide synthesis stimulating factors from the dorsal bodies and cerebral ganglia of Helisoma duryi (Mollusca: Pulmonata)

1988 ◽  
Vol 66 (2) ◽  
pp. 508-511 ◽  
Author(s):  
S. L. Miksys ◽  
A. S. M. Saleuddin
Keyword(s):  
The Other ◽  
Dependent Manner ◽  
Endocrine Control ◽  
Cerebral Ganglia ◽  
Helisoma Duryi ◽  
Polysaccharide Synthesis ◽  
Two Factors ◽  
Dose Dependent ◽  
Stimulating Factor

Polysaccharide synthesis by the albumen gland of Helisoma duryi is under the endocrine control of two factors, one from the cerebral ganglia and the other from the dorsal bodies. Both factors stimulate albumen synthesis in vitro in a dose-dependent manner. The cerebral ganglia derived factor is probably a peptide or protein, as it is not extracted by acid or base and is sensitive to trypsin. The nature of the dorsal body derived polysaccharide synthesis stimulating factor is less clear. It is heat labile and not extracted by acid or base, but is insensitive to trypsin and general proteases, and is soluble in methanol. These characteristics lend some support to the hypothesis that dorsal body hormone is a steroid.

Neurosecretion and shell regeneration in Helisoma duryi (Mollusca: Pulmonata)

1976 ◽  
Vol 54 (10) ◽  
pp. 1771-1778 ◽  
Author(s):  
R. M. Dillaman ◽  
A. S. M. Saleuddin ◽  
Gwyneth M. Jones
Keyword(s):  
Tissue Injury ◽  
Secretory Granules ◽  
Neurosecretory Cells ◽  
Neurosecretory Material ◽  
Positive Material ◽  
Helisoma Duryi ◽  
Cell Groups ◽  
Shell Regeneration ◽  
The Brain ◽  
Group 1

The distribution of cells that stain positively with paraldehyde fuchsin (PAF) in the brain of Helisoma duryi has been mapped. Two cell groups occur in the visceral ganglion, one of which (group 1) has been shown to contain electron-dense secretory granules resembling neurosecretory material. Backfilling experiments and serial sectioning show that group 1 cells send axons into the intestinal and left pallial nerves.PAF-positive material is depleted from group 1 cells between 24 and 48 h after shell removal and has reappeared by 72 h. PAF-positive material accumulates in the axons by 24 h and disappears by 48 h; shell regeneration is apparently complete within 15 days. Tissue injury alone or shell removal from a non-regenerating area does not cause depletion of stainable material in these cells. No change in stainability occurs in other PAF-positive cells in the brain during shell regeneration. It is suggested that this cycle of activity may indicate involvement of the group 1 neurosecretory cells in the control of shell regeneration.

scholarly journals Development and histological characteristics of neurosecretory cells in the cerebral ganglia of Achatina fulica (Bowdich)

1994 ◽  
Vol 15 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Maleeya Kruatrachue ◽  
Viyada Seehabutr ◽  
Jittipan Chavadej ◽  
Prapee Sretarugsa ◽  
E. Suchart Upatham ◽  
...  
Keyword(s):  
Neurosecretory Cells ◽  
Achatina Fulica ◽  
Cerebral Ganglia ◽  
Histological Characteristics

Mating increases the synthetic activity of the neurosecretory caudodorsal cells of Helisoma duryi (Mollusca: Pulmonata)

1989 ◽  
Vol 67 (10) ◽  
pp. 2363-2367 ◽  
Author(s):  
S. T. Mukai ◽  
A. S. M. Saleuddin
Keyword(s):  
Light Microscope ◽  
Egg Laying ◽  
Microscope Level ◽  
Synthetic Activity ◽  
Leucine Incorporation ◽  
Light Microscope Level ◽  
Cerebral Ganglia ◽  
Helisoma Duryi

In Helisoma duryi, virgin snails lay significantly fewer eggs than mated snails. It is generally accepted that in basommatophoran pulmonates, the neurosecretory caudodorsal cells located in the cerebral ganglia produce a hormone that regulates egg laying. The synthetic activity of the caudodorsal cells in Helisoma has been measured in vitro and in vivo using tritiated leucine. Virgin and castrated snails (reproductively inactive) showed significantly reduced levels of [3H]leucine incorporation compared with first-mated snails (24 and 48 h postmating). This increase in synthetic activity following mating was corroborated by autoradiography at the light microscope level which localized transport of caudodorsal cell neurosecretory proteins to the cerebral commissure, the neurohaemal area. Mating triggers an increase in synthetic activity of the caudodorsal cells.

Studies on the in vitro formation of periostracum in Helisoma duryi: the influence of the dorsal epithelium on calcium incorporation

1984 ◽  
Vol 62 (6) ◽  
pp. 1177-1180 ◽  
Author(s):  
S. C. Kunigelis ◽  
A. S. M. Saleuddin
Keyword(s):  
Epithelial Tissue ◽  
Whole Brain ◽  
Fast Growing ◽  
Cerebral Ganglia ◽  
Helisoma Duryi ◽  
Shell Deposition ◽  
Slow Growing

Mantle collar tissue was found to produce periostracum when placed in vitro. The rate of shell deposition in vivo was reflected in the in vitro rate of periostracum formation. The addition of whole brain from fast-growing donors to mantle collar from slow-growing animals was found to increase the amount of periostracum produced in vitro. This effect was further enhanced by removing the cerebral ganglia lateral lobes prior to incubation. The presence of dorsal epithelial tissue was found to increase the incorporation of calcium into periostracum formed in vitro.

Identification of insulin-like peptides in cerebral ganglia neurosecretory cells of the mussel Mythus edulis

1994 ◽  
Vol 26 (6) ◽  
pp. 891-899 ◽  
Author(s):  
Kristell Kellner-Cousin ◽  
Eric Mialhe ◽  
Michel Mathieu
Keyword(s):  
Neurosecretory Cells ◽  
Cerebral Ganglia

Extirpation and transplantation of the brain of the snail Helix aspersa: a study of the survival of the animal and the implant

1990 ◽  
Vol 68 (7) ◽  
pp. 1505-1512 ◽  
Author(s):  
Philippe Gomot ◽  
Lucien Gomot ◽  
Claude-Roland Marchand ◽  
Claude Colard
Keyword(s):  
Neurosecretory Cells ◽  
Helix Aspersa ◽  
Culture Methods ◽  
Cerebral Ganglia ◽  
Temperature Regimes ◽  
In Vivo Culture ◽  
Immunocytochemical Studies ◽  
Snail Helix Aspersa ◽  
The Brain

Dormant, hibernating snails whose brains (supraoesophageal cerebral ganglia and associated dorsal bodies) were removed by surgery continued to live retracted in their shells. The remaining ganglia of the circumoesophageal ring did not regenerate in the 12 months following the removal of the brain. The transplanted brains in the haemocoel (auto- or allo-graft) were not rejected and many of their cerebral neurosecretory cells and the endocrine dorsal bodies appeared normal structurally and functionally, as evidenced by ultrastructural and immunocytochemical studies. The data from these experiments demonstrate the possibility of studying by in vivo culture methods the roles of cerebral ganglia and endocrine dorsal bodies on the control of reproduction under different temperature regimes.

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