Time course of uptake, elimination, and tissue levels of [14C]DDT in wood-frog tadpoles

1976 ◽  
Vol 54 (3) ◽  
pp. 355-360 ◽  
Author(s):  
Lawrence E. Licht
Keyword(s):  
Time Course ◽  
Rana Sylvatica ◽  
Post Treatment ◽  
Wood Frog ◽  
Tissue Levels ◽  
Frog Tadpoles ◽  
Residue Levels ◽  
Pesticide Deposition

Rana sylvatica tadpoles were treated with 0.001 and 0.003 ppm [14C]DDT (1,1,1 -trichloro-2,2-bis(p-chlorophenyl)ethane) at 15 and 21 °C and the time course of uptake and residue levels in several tissues of contaminated tadpoles were studied. By 24 h post treatment, highest concentrations of DDT were found in whole animals and in isolated tadpole tissues. After 80 h, DDT tissue levels began to diminish and fall slowly throughout the duration of the experiment. Liver and fat were the main sites for pesticide deposition. Tadpoles treated at 15 °C retained higher tissue levels of DDT for longer periods, i.e., they lost DDT more slowly than tadpoles at 21 °C.

Actue Toxic Effects of Round-Up Herbicide on Wood Frog Tadpoles (Rana sylvatica)

2007 ◽  
Vol 22 (4) ◽  
pp. 705-708 ◽  
Author(s):  
Brett A. Comstock ◽  
Spencer L. Sprinkle ◽  
Geoffrey R. Smith
Keyword(s):  
Rana Sylvatica ◽  
Wood Frog ◽  
Toxic Effects ◽  
Frog Tadpoles

scholarly journals Survey of mercury in boreal chorus frog (Pseudacris maculata) and wood frog (Rana sylvatica) tadpoles from wetland ponds in the Prairie Pothole Region of Canada

FACETS ◽  
2017 ◽  
Vol 2 (1) ◽  
pp. 315-329 ◽  
Author(s):  
Stacy A. Boczulak ◽  
Mark C. Vanderwel ◽  
Britt D. Hall
Keyword(s):  
Prairie Pothole Region ◽  
Body Burden ◽  
Predictive Ability ◽  
Rana Sylvatica ◽  
Trophic Levels ◽  
Wood Frog ◽  
Prairie Pothole ◽  
Pseudacris Maculata ◽  
Frog Tadpoles ◽  
Chorus Frog

Tadpoles are important prey items for many aquatic organisms and often represent the largest vertebrate biomass in many fishless wetland ecosystems. Neurotoxic mercury (Hg) can, at elevated levels, decrease growth, lower survival, and cause developmental instability in amphibians. We compared total Hg (THg) body burden and concentration in boreal chorus frog ( Pseudacris maculata) and wood frog ( Rana sylvatica) tadpoles. Overall, body burden and concentration were lower in boreal chorus frog tadpoles than wood frog tadpoles, as expected, because boreal chorus frog tadpoles consume at lower trophic levels. The variables species, stage, and mass explained 21% of total variation for body burden in our models but had negligible predictive ability for THg concentration. The vast majority of the remaining variation in both body burden and THg concentration was attributable to differences among ponds; tadpoles from ponds in three areas had considerably higher THg body burden and concentration. The pond-to-pond differences were not related to any water chemistry or physical parameter measured, and we assumed that differences in wetland geomorphology likely played an important role in determining Hg levels in tadpoles. This is the first report of Hg in frog tadpoles in the Prairie Pothole Region of North America.

Kin recognition in wood frog tadpoles (Rana sylvatica): factors affecting spatial proximity to siblings

1991 ◽  
Vol 69 (10) ◽  
pp. 2569-2571 ◽  
Author(s):  
Sarah A. Rautio ◽  
Elizabeth A. Bura ◽  
Keith A. Berven ◽  
George J. Gamboa
Keyword(s):  
Kin Recognition ◽  
Rana Sylvatica ◽  
Wood Frog ◽  
Spatial Proximity ◽  
Factors Affecting ◽  
Recognition Ability ◽  
Frog Tadpoles ◽  
Sibling Recognition

In blind laboratory observations, we investigated three factors affecting spatial proximity to full siblings in wood frog (Rana sylvatica) tadpoles. In the first set of observations, we provided tadpoles the opportunity to associate with (i) siblings or no larvae and (ii) nonkin or no larvae. Tadpoles associated preferentially with siblings over no larvae, but showed no preference for either nonkin or no larvae. Thus tadpoles associate with siblings because they are attracted to kin rather than repulsed by nonkin. In a second set of observations, 10-day-old tadpoles failed to display sibling recognition, while 17-day-old tadpoles displayed a significant sibling preference. Furthermore, 17-day-old tadpoles spent significantly more time with siblings than did 10-day-old tadpoles. Thus, age affects sibling preference in wood frog tadpoles. When retested several days later, the former 17-day-old tadpoles again displayed a significant kin preference. However, there was no significant correlation in kin preference between a tadpole's first and second test. This and another comparison indicate that all wood frog tadpoles possess recognition ability, and that the failure of some tadpoles to manifest sibling preference is not due to a lack of recognition ability.

Growth of the Wood Frog, Rana sylvatica

Copeia ◽  
1961 ◽  
Vol 1961 (1) ◽  
pp. 74 ◽  
Author(s):  
Edward D. Bellis
Keyword(s):  
Rana Sylvatica ◽  
Wood Frog

Distribution of the middorsal stripe dimorphism in the wood frog, Rana sylvatica, in eastern North America

1980 ◽  
Vol 58 (9) ◽  
pp. 1643-1651 ◽  
Author(s):  
Frederick W. Schueler ◽  
Francis R. Cook
Keyword(s):  
United States ◽  
North America ◽  
Rana Sylvatica ◽  
Wood Frog ◽  
Eastern North America ◽  
Hudson Bay ◽  
Northeastern United States ◽  
Southern Ontario ◽  
Maritime Canada ◽  
Eurasian Species

The frequency of the middorsally striped morph of Rana sylvatica in Ontario and Manitoba varies from absence in southern Ontario to 80% on the coast of Hudson Bay, with a general value of 20–30% in the boreal forest, a rise to 50% on the forest–grassland ecotone in southern Manitoba, and a decline westward to 20% on the edge of the prairies. This morph is rare in the northeastern United States and Maritime Canada. The suggested relationship between its frequency and the "grassiness" of the background on which predators view it is reexamined, and it is suggested that a linkage with earlier transformation as demonstrated in Eurasian species may explain certain anomalies.

Freeze-thaw injury in erythrocytes of the freeze-tolerant wood frog, Rana sylvatica

1991 ◽  
Vol 261 (6) ◽  
pp. R1346-R1350 ◽  
Author(s):  
J. P. Costanzo ◽  
R. E. Lee
Keyword(s):  
Cell Injury ◽  
Osmotic Fragility ◽  
Rana Sylvatica ◽  
Freeze Tolerance ◽  
Wood Frog ◽  
In Vitro Tests ◽  
Freezing And Thawing ◽  
Freeze Tolerant ◽  
High Concentrations

Erythrocytes from the freeze-tolerant wood frog (Rana sylvatica) were subjected to in vitro tests of freeze tolerance, cryoprotection, and osmotic fragility. The responses of cells from frogs acclimated to 4 or 15 degrees C were similar. Erythrocytes that were frozen in saline hemolyzed at -4 degrees C or lower. The addition of high concentrations (150 and 1,500 mM) of glucose or glycerol, cryoprotectants produced naturally by freeze-tolerant frogs, significantly reduced cell injury at -8 degrees C, but concentrations of 1.5 or 15 mM were ineffective. Hemolysis was reduced by 94% with 1,500 mM glycerol and by 84% with 1,500 mM glucose; thus glycerol was the more effective cryoprotectant. Mean fragility values for frog erythrocytes incubated in hypertonic and hypotonic saline were 1,938 and 49 mosM, respectively. Survival in freeze tolerance and cryoprotection experiments was comparable for erythrocytes from frogs and humans, suggesting that these cells may respond similarly to freezing-related stresses. However, the breadth of osmotic tolerance, standardized for differences in isotonicity, was greater for frog erythrocytes than for human erythrocytes. Our data suggest that erythrocytes from R. sylvatica are adequately protected by glucose under natural conditions of freezing and thawing.

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