Measurement of circulating thyroxine in several freshwater teleosts by competitive binding analysis

1973 ◽  
Vol 51 (1) ◽  
pp. 49-53 ◽  
Author(s):  
D. A. Higgs ◽  
J. G. Eales
Keyword(s):  
Brook Trout ◽  
Competitive Binding ◽  
Freshwater Species ◽  
Thyroxine Binding Globulin ◽  
Binding Analysis ◽  
Freshwater Teleosts

A competitive binding analysis using human thyroxine-binding globulin (TBG) and Sephadex columns was the most satisfactory of several methods tested for measuring fish plasma thyroxine concentrations.Thyroxine levels were usually less than 1 μg and in most cases less than 0.5 μg per 100 ml plasma for laboratory-held brook trout and nine other freshwater species.

Serum Theophylline by Fluorescence Polarisation Immunoassay

1984 ◽  
Vol 21 (6) ◽  
pp. 523-526 ◽  
Author(s):  
Russell M Young ◽  
M J Smith ◽  
R B Payne
Keyword(s):  
Competitive Binding ◽  
Serum Theophylline ◽  
Drug Levels ◽  
Binding Analysis ◽  
Fluorescence Polarisation

The Abbott TDX system utilises the principles of fluorescence polarisation and competitive binding analysis for the quantitation of drug levels in plasma. The system proved easy to use, and produced reliable results rapidly. The high cost of reagents is balanced by the fact that the reagents are stable and therefore the assays can be calibrated infrequently.

scholarly journals The estimation of serum thyroxine by competitive binding analysis: A modified method

1974 ◽  
Vol 27 (1) ◽  
pp. 74-82 ◽  
Author(s):  
D. J. Goldie ◽  
R. D. Jennings ◽  
G. K. McGowan
Keyword(s):  
Competitive Binding ◽  
Serum Thyroxine ◽  
Modified Method ◽  
Binding Analysis

scholarly journals Annual and Monthly Variation in Species Composition and Catches of Fishes from the Tabusintac River Estuary in the Southern Gulf of St. Lawrence

2009 ◽  
Vol 123 (1) ◽  
pp. 48 ◽  
Author(s):  
A. M. Herrell ◽  
D. A. Methven
Keyword(s):  
Atlantic Salmon ◽  
Species Composition ◽  
Brook Trout ◽  
White Perch ◽  
River Estuary ◽  
Fish Fauna ◽  
Freshwater Species ◽  
Total Catch ◽  
Additional Species ◽  
Sampling Gear

A total of 7130 fish from 13 taxa were collected during summer and autumn, 2002-2005 using box traps at two sites in the Tabusintac River Estuary, New Brunswick, Canada. The objective was to determine seasonal change in species composition and catches, and to access change in annual returns of Atlantic Salmon, an important recreational fishery during autumn on the Tabusintac, Miramichi and neighboring rivers. Taxa richness and composition varied annually and from trap to trap. Richness ranged from 8-10 taxa annually and catches were usually highest in the trap closest to the ocean despite its slightly smaller size. Taxa contributing greater than one percent of the total catch during the four years of sampling included six diadromous taxa: Blueback Herring and Alewife (41.65%), Striped Bass (21.54%), Atlantic Salmon (3.98%), Tomcod (2.95%), Brook Trout (1.05%), American Eel (5.27%); one freshwater species, White Sucker (11.96%); one marine species, Winter Flounder and one resident estuarine species, Smooth Flounder which together account for 11.19% of the total catch. The remaining five species (American Smelt, Northern Pipefish, White Perch, Cunner, Sea Lamprey) contributed 0.39% of the total catch. The fish fauna 14-15 km up the Tabusintac River was not species rich, due in large part to the limited sampling time each year and due to fishing with just one sampling gear, a stationary box trap. Additional species would have been collected if smaller mesh sampling gear were used. Comparisons were made with other studies and the phenology of the dominant species on the Tabusintac and Miramichi River Estuaries during the ice free season is established. Most catches appear to be related to pre- or post-spawning movements of anadromous fishes.

Transient Prealbumin- Associated Hyperthyroxinemia in TSH-Producing Pituitary Adenoma

1990 ◽  
Vol 29 (01) ◽  
pp. 40-43 ◽  
Author(s):  
W. Langsteger ◽  
P. Költringer ◽  
P. Wakonig ◽  
B. Eber ◽  
M. Mokry ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Case Report ◽  
Pituitary Adenoma ◽  
Thyroid Hormones ◽  
Protein Binding ◽  
Alpha Subunit ◽  
Normal Range ◽  
Thyroxine Binding Globulin ◽  
Free Thyroid Hormones ◽  
Transmission Computed Tomography

This case report describes a 38-year-old male who was hospitalized for further clarification of clinically mild hyperthyroidism. His increased total hormone levels, the elevated free thyroid hormones and the elevated basal TSH with blunted response to TRH strongly suggested a pituitary adenoma with inappropriate TSH incretion. Transmission computed tomography showed an intrasellar expansion, 16 mm in diameter. The neoplastic TSH production was confirmed by an elevated alpha-subunit and a raised molar alpha-sub/ATSH ratio. However, T4 distribution on prealbumin (PA, TTR), albumin (A) and thyroxine binding globulin (TBG) showed a clearly increased binding to PA (39%), indicating additional prealbumin-associated hyperthyroxinemia. The absolute values of PA, A and TBG were within the normal range. After removal of the TSH-producing adenoma, basal TSH, the free thyroid hormones and T4 binding to prealbumin returned to normal. Therefore, the prealbumin-associated hyperthyroxinemia had to be interpreted as a transitory phenomenon related to secondary hyperthyroidism (T4 shift from thyroxine binding globulin to prealbumin) rather than a genetically conditioned anomaly of protein binding.

EVALUATION OF A SIMPLIFIED METHOD FOR DETERMINING SERUM THYROXINE BY COMPETITIVE PROTEIN BINDING ANALYSIS

1970 ◽  
Vol 64 (4) ◽  
pp. 630-636 ◽  
Author(s):  
Stephen C. Thorson ◽  
Ronald Tsujikawa ◽  
James L. Brown ◽  
Robert T. Morrison ◽  
Hamish W. McIntosh
Keyword(s):  
Protein Binding ◽  
Clinical Application ◽  
Thyroid Status ◽  
Serum Thyroxine ◽  
Binding Analysis ◽  
Simplified Method ◽  
Competitive Protein Binding ◽  
Hypothyroid Patients

ABSTRACT Serum thyroxine concentrations were determined in 66 euthyroid, 30 hyperthyroid and 13 hypothyroid patients using both the established Murphy method and a simplified method of competitive protein binding analysis. A diagnosis compatibility of 96% was found with both methods indicating that the simplified method has comparable clinical application as an initial screen of thyroid status.

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