Blood cell destruction in the opisthonephric kidney of the sea lamprey, Petromyzon marinus L.

1971 ◽  
Vol 49 (6) ◽  
pp. 962-963 ◽  
Author(s):  
John H. Youson
Keyword(s):  
Blood Cell ◽  
Blood Cells ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Electron Microscopic ◽  
Cell Destruction

Electron microscopic observations reveal that blood cells are engulfed by macrophages within interstitial sinusoids of the opisthonephric kidneys of larval and adult Petromyzon marinus. Therefore the kidneys represent one site where blood cell destruction may occur in this animal.

Haemocytology of the supraneural myeloid body in the sea lamprey during several phases of life cycle

1974 ◽  
Vol 52 (12) ◽  
pp. 1585-1589 ◽  
Author(s):  
J. C. George ◽  
F. W. H. Beamish
Keyword(s):  
Blood Cells ◽  
Petromyzon Marinus ◽  
Cell Types ◽  
Sea Lamprey ◽  
Histochemical Staining ◽  
Fat Cells ◽  
Cell Type ◽  
Adult Tissue ◽  
Spawning Run ◽  
Early Phases

The supraneural myeloid body of the sea lamprey (Petromyzon marinus) was studied in the feeding adult, late spawning run adult, and metamorphosing ammocoete. The fatty nature of the tissue was established by histochemical staining and electron microscopy. The presence of the fat cells and the actively differentiating blood cells evinced its similarity to the bone marrow in higher animals, thereby suggesting a phylogenetic affinity. In the late spawning run lampreys, the tissue was found to be almost empty of blood cells, leaving empty spaces within the stromal skeleton. In the feeding adult tissue, the various blood cell types differentiated from precursor cells have been identified. Megakaryoblasts possibly representing early phases of the cell type were observed only in the transforming (macrophthalmia stage) and adult lampreys. A marked active development of the tissue in the ammocoete was seen only at the fourth stage of the metamorphosing ammocoete immediately before macrophthalmia. At the macrophthalmia stage, the haematopoietic activity in the tissue increased substantially.

In vitro evidence for sodium-dependent pH regulation in sea lamprey (Petromyzon marinus) red blood cells

1992 ◽  
Vol 70 (3) ◽  
pp. 411-416 ◽  
Author(s):  
Bruce L. Tufts
Keyword(s):  
Carbon Dioxide ◽  
Blood Cells ◽  
Ph Regulation ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Carbon Dioxide Tension ◽  
Factors Influencing ◽  
Rate Of Increase ◽  
Sodium Dependent

Factors influencing the pH of sea lamprey (Petromyzon marinus) erythrocytes were examined in vitro. The absence of extracellular Na+ caused a significant reduction in the erythrocyte pH. In addition, the protonophore 2,4-dinitrophenol was capable of reducing the erythrocyte pH when it was dissolved in dimethyl sulfoxide. In the presence of ouabain, a step increase in the carbon dioxide tension caused a large increase in the intracellular Na+ concentration, but the rate of increase was considerably reduced after the 1st hour. Even in the absence of ouabain, however, the intracellular Na+ concentration in erythrocytes equilibrated with 3% CO2 is much greater than that in erythrocytes equilibrated with 0.2% CO2. Together, these results suggest that Na+-dependent H+ movements, possibly Na+–H+ exchange, may have an important role in erythrocyte pH regulation in P. marinus. Moreover, the mechanism appears to be stimulated by the decrease in extracellular or erythrocyte pH associated with the increase in [Formula: see text]. Extracellular Na+ also has a significant impact on the CO2-transport properties of P. marinus blood. In the absence of extracellular Na+, the intracellular total CO2 concentration was significantly reduced, whereas extracellular total CO2 concentration, [Formula: see text], was significantly increased. Furthermore, in the no-Na+ saline, [Formula: see text] became dependent on the hematocrit; an increase in the number of erythrocytes resulted in an increase in [Formula: see text]. This result suggests that the erythrocyte membrane of P. marinus may be permeable to [Formula: see text].

scholarly journals IN VITRO INTERACTIONS BETWEEN OXYGEN AND CARBON DIOXIDE TRANSPORT IN THE BLOOD OF THE SEA LAMPREY (PETROMYZON MARINUS)

1992 ◽  
Vol 173 (1) ◽  
pp. 25-41 ◽  
Author(s):  
R. A. Ferguson ◽  
N. Sehdev ◽  
B. Bagatto ◽  
B. L. Tufts
Keyword(s):  
Carbon Dioxide ◽  
Blood Cell ◽  
Red Blood Cells ◽  
Red Blood Cell ◽  
Whole Blood ◽  
Blood Cells ◽  
Sea Lamprey ◽  
Carbon Dioxide Transport ◽  
Dissociation Curves

In vitro experiments were carried out to examine the interactions between oxygen and carbon dioxide transport in the blood of the sea lamprey. Oxygen dissociation curves for whole blood obtained from quiescent lampreys had Hill numbers (nH) ranging from 1.52 to 1.89. The Bohr coefficient for whole blood was -0.17 when extracellular pH (pHe) was considered, but was much greater (-0.63) when red blood cell pH (pHi) was considered. The pHi was largely dependent on haemoglobin oxygen- saturation (SO2) and the pH gradient across the red blood cell membrane was often reversed when PCO2 was increased and/or SO2 was lowered. The magnitude of the increase in pHi associated with the Haldane effect ranged from 0.169 pH units at 2.9 kPa PCO2 to 0.453 pH units at a PCO2 of 0.2 kPa. Deoxygenated red blood cells had a much greater total CO2 concentration (CCO2) than oxygenated red blood cells, but the nonbicarbonate buffer value for the red blood cells was unaffected by oxygenation. Plasma CCO2 was not significantly different under oxygenated or deoxygenated conditions. Partitioning of CO2 carriage in oxygenated and deoxygenated blood supports recent in vivo observations that red blood cell CO2 carriage can account for much of the CCO2 difference between arterial and venous blood. Together, the results also suggest that oxygen and carbon dioxide transport may not be tightly coupled in the blood of these primitive vertebrates. Finally, red cell sodium concentrations were dependent on oxygen and carbon dioxide tensions in the blood, suggesting that sodium-dependent ion transport processes may contribute to the unique strategy for gas transport in sea lamprey blood.

Blood Cell Lineage in the Sea Lamprey, Petromyzon marinus (Pisces: Petromyzontidae)

Copeia ◽  
1971 ◽  
Vol 1971 (4) ◽  
pp. 722 ◽  
Author(s):  
George W. Piavis ◽  
James L. Hiatt
Keyword(s):  
Blood Cell ◽  
Petromyzon Marinus ◽  
Cell Lineage ◽  
Sea Lamprey

Structure of the conus arteriosus and ventral aorta in the sea lamprey, Petromyzon marinus, and the Atlantic hagfish, Myxine glutinosa: microfibrils, a major component

1984 ◽  
Vol 62 (12) ◽  
pp. 2445-2456 ◽  
Author(s):  
Glenda M. Wright
Keyword(s):  
Extracellular Matrix ◽  
Connective Tissue ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Elastic Fibre ◽  
Electron Microscopic ◽  
Primitive Form ◽  
Myxine Glutinosa ◽  
Ventral Aorta ◽  
Adult Lamprey

Light and electron microscopic observations of the conus arteriosus (CA) and ventral aorta (VA) of the sea lamprey, Petromyzon marinus, and the Atlantic hagfish, Myxine glutinosa, reveal the presence of a unique form of fibrous extracellular matrix within their media and an unusual type of connective tissue forming the lateral subendothelial distensions found only in the lamprey CA. The extracellular matrix of the media in lamprey and hagfish CA and VA is characterized by large concentrations of tubular, elastic fibrelike microfibrils 11–17 nm in diameter. No elastic fibrelike elastin component could be found. Collagen fibrils, matrix granules, and intergranular filaments make up the remainder of the matrix. The loose connective tissue composing the lateral distensions of the larval and adult lamprey CA contains ultrastructural features similar to those of lamprey mucocartilage. It is speculated that since lampreys and hagfish are living representatives of the most primitive group of vertebrates, the microfibrils present in their major arteries may represent a primitive form of elastic fibre in which elastin has not yet developed. The functional significance of the extracellular microfibrils in the CA and VA of lamprey and hagfish and the occurrence of lateral distensions in the lamprey CA are discussed.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

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