Demonstration of intranuclear vesicles in blast cells in lymph nodes with different fixatives and staining techniques

1969 ◽  
Vol 47 (6) ◽  
pp. 1414-1416 ◽  
Author(s):  
Jessica L. G. Shelley ◽  
Jean E. Mills Westermann ◽  
Vibeke E. Engelbert
Keyword(s):  
Lymph Nodes ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Blast Cell ◽  
Iodine Vapor ◽  
Cell Nuclei ◽  
Blast Cells ◽  
Staining Methods ◽  
Staining Techniques ◽  
Haematoxylin Staining

For the demonstration or intranuclear vesicles, iodine vapor, formalin vapor, and methyl alcohol fixations give equivalent results when followed by staining with the May-Grünwald Giemsa technique. Neutral buffered formalin and May-Grünwald Giemsa staining demonstrate fewer intranuclear vesicles than the first-mentioned combinations. Iodine vapor or formalin vapor fixations followed by Mayer's haematoxylin staining show greater numbers of nuclei with vesicles.Formalin fixes nuclei slowly, which allows nuclear contraction and thus the squeezing-out of vesicles. Haematoxylin stains protein, while May-Grünwald Giemsa technique does not stain proteins but deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). It therefore follows that the demonstration of vesicles in blast cell nuclei depends on the fixatives and staining methods used.

MORPHOLOGY OF BLAST CELL NUCLEI IN VIVO AND IN VITRO

1962 ◽  
Vol 40 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Vibeke E. Engelbert ◽  
Donald B. McMillan
Keyword(s):  
Blast Cell ◽  
Cell Nuclei ◽  
Normal Pattern ◽  
Natural Function ◽  
Rounded Form ◽  
History Of ◽  
Blast Cells ◽  
Twisted Shape

This paper describes and illustrates the behavior of blast cell nuclei recorded by a motion picture camera through a phase contrast microscope. This behavior includes extension and contraction of the nuclei to present the classically accepted patterns of a rounded nucleus at one moment and an elongated often twisted shape a few seconds later. The motion involved is not amoeboid action. The elongated nucleus is not accepted in the literature as a normal pattern of a blast cell nucleus but it obviously should be. After an extension the nucleus contracts again presenting the classical rounded form commonly associated with blast cells.The authors believe that this nuclear behavior is a natural function of the blast cell and that it occurs many times during the life history of this cell. It is especially characteristic of the time when the blast cell is releasing most of its nuclear contents to form free basic nuclear units and other components of the blood. A comparison is made with similar blast cells and their nuclear variations in a Feulgen "gentle squash" preparation which is lightly counterstained with fast green F.C.F.

High Fibrinopeptide A (FPA) Levels in Acute Non-Lymphocytic Leukemia Are Reduced by Heparin Administration

1984 ◽  
Vol 52 (03) ◽  
pp. 301-304 ◽  
Author(s):  
L Gugliotta ◽  
Silvana Viganò ◽  
A D’Angelo ◽  
Anna Guarini ◽  
S Tura ◽  
...  
Keyword(s):  
Body Temperature ◽  
Blast Cell ◽  
Lymphocytic Leukemia ◽  
Healthy Controls ◽  
Intravenous Bolus ◽  
Fibrinopeptide A ◽  
Cell Counts ◽  
Heparin Administration ◽  
Blast Cells ◽  
Baseline Values

SummaryPlasma levels of fibrinopeptide A (FPA) in 30 untreated patients with acute non-lymphocytic leukemia (ANLL) were significantly higher than in 30 healthy controls (p <0.001). Patients without laboratory signs of disseminated intravascular coagulation (DIC) had levels of FPA higher than controls (p <0.02) but markedly lower than patients with DIC (p <0.001). Five patients with M3 leukemia had a higher mean FPA level (p <0.02) and a lower peripheral blast cell count (p <0.05) than patients with other cytological subtypes of ANLL. When patients with M3 were excluded, a significant correlation was observed between the peripheral blast cell counts and the FPA levels (r = 0.66, p <0.001). FPA levels were similar with body temperature either above or below 38° C. After intravenous bolus of heparin FPA dropped to normal levels in 14 out of 17 patients who had high baseline values. These findings indicate that intravascular thrombin formation, which probably result from the expression of procoagulant activities of blast cells, is the main cause of high FPA in the majority of patients with acute non-lymphocytic leukemia.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
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