A non-histological method for determining the completeness of hypophysectomy in the goldfish (Carassius auratus)

1969 ◽  
Vol 47 (4) ◽  
pp. 324-325 ◽  
Author(s):  
P. H. Johansen ◽  
A. W. Roy

Experiments show that the loss of fin color of the goldfish correlates perfectly with the complete removal of the pituitary gland, and this color loss can be used as a criterion for determining the completeness of hypophysectomy.

1973 ◽  
Vol 51 (12) ◽  
pp. 1289-1291 ◽  
Author(s):  
P. H. Johansen ◽  
J. D. Gomery

After either pituitary removal or pituitary autotransplantation, the routine oxygen consumption of goldfish is reduced significantly from control levels. A similar pattern appears to be reflected by the oxygen consumption of white muscle tissue.


1962 ◽  
Vol 38 (7) ◽  
pp. 345-347 ◽  
Author(s):  
Nobuo EGAMI ◽  
Hisami ETOH ◽  
Chikashi TACHI ◽  
Kazuko AOKI ◽  
Ryoichi ARAI

2007 ◽  
Vol 77 (Suppl_1) ◽  
pp. 185-185
Author(s):  
E Zhao ◽  
Colin Cameron ◽  
Andrew Han Wen Chen ◽  
Ajoy Basak ◽  
Anderson On- Lam Wong ◽  
...  

1975 ◽  
Vol 53 (9) ◽  
pp. 1306-1323 ◽  
Author(s):  
K. H. Khoo

This investigation of the role of pre- and post-ovulatory corpus luteum provided a better understanding of the various functions of follicular tissues in goldfish ovaries. When yolky oocytes become atretic, either naturally or under adverse conditions, the granulosa cells hypertrophy and serve a phagocytic function. This is evident in the α and β stages of the preovulatory corpus luteum. In addition to the removal of atretic oocytes, the follicular cells are involved in steroidogenesis, as demonstrated by the detection of hydroxysteroid dehydrogenases in the granulosa of normal oocytes and the corpus luteum, both pre- and post-ovulatory. In the preovulatory corpus luteum the steroidogenic function was evident only after the complete removal of the atretic oocyte. Hence, steroidogenesis was observed only in the γ stage preovulatory corpus luteum. In the postovulatory follicles, steroidogenesis was evident immediately after ovulation. It is postulated that the steroid hormones synthesized by the pre- and post-ovulatory corpus luteum induced some of the corpora lutea cells to differentiate into oogonia. There are two major lines of evidence, one histological and the other from autoradiography, which suggest that corpora lutea cells ultimately differentiate into oogonia.


1976 ◽  
Vol 54 (11) ◽  
pp. 1960-1968 ◽  
Author(s):  
B. A. McKeown ◽  
R. E. Peter

A number of experiments were conducted to investigate the effects of photoperiod and temperature on prolactin release from the goldfish pituitary gland. Fish were acclimated to different photoperiods and temperatures, and also were subjected to a change in either of these two parameters after different acclimation conditions. Serum and pituitary samples were collected and analyzed by radioimmunoassay for prolactin levels. In other experiments samples for prolactin analysis were taken every 3 h intermittently over a period of 3 days from fish that were acclimated to different photoperiod and temperature conditions. Longer photoperiods and higher temperatures caused pituitary prolactin release. Serum prolactin changed on a circadian rhythm and the rhythm was modified depending on the length of the photoperiod.


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