Variations in the photoperiodic response of Neodiprion sertifer

1968 ◽  
Vol 46 (5) ◽  
pp. 1082-1083 ◽  
Author(s):  
C. R. Sullivan ◽  
D. R. Wallace

Natural populations of N. sertifer, located at about the same latitude in Ontario, differ considerably in their response to photoperiods inducing non-diapause when reared as larvae at identical temperatures. In addition, within a single population, the incidence of non-diapause varies inversely with larval rearing temperature.

1968 ◽  
Vol 46 (5) ◽  
pp. 959-963 ◽  
Author(s):  
C. R. Sullivan ◽  
D. R. Wallace

Hard, dark-red, tumor-like inclusions that are apparently unrelated to nuclear polyhedrosis have been detected in adult Neodiprion sertifer. The frequency of occurrence of these structures is related to larval rearing temperature. They are consistently more common in adults that have undergone prepupal diapause than in adults that have not had a prepupal diapause. Differences in the incidence of the inclusions occur between families, and the bodies are more commonly found in females than in males.


1998 ◽  
Vol 46 (4) ◽  
pp. 533 ◽  
Author(s):  
Siegfried L. Krauss ◽  
Rod Peakall

The accurate assignment of paternity in natural plant populations is required to address important issues in evolutionary biology, such as the factors that affect reproductive success. Newly developed molecular fingerprinting techniques offer the potential to address these aims. Here, we evaluate the utility of a new PCR-based multi-locus fingerprinting technique called Amplified Fragment Length Polymorphism (AFLP) for paternity studies in Persoonia mollis (Proteaceae). AFLPs were initially scored for five individuals from three taxonomic levels for 64 primer pairs: between species (P. mollis and P. levis), between subspecies (P. mollis subsp. nectens and subsp. livens), between individuals within a single population of P. mollis, as well as for a naturally pollinated seed from a single P. mollis subsp. nectens plant. Overall, 1164 fragments (24.6% of all fragments) were polymorphic between species, 743 (16.5%) between subspecies, 371 (8.6%) between individuals within a single population, and 265 (6.2%) between a plant and its seed. Within a single P. mollis population of 14 plants, 42 polymorphic fragments were scored from profiles generated by a single AFLP primer pair. The mean frequency of the recessive allele (q) over these 42 loci was 0.773. Based on these observations, it will be feasible to generate well over 100 polymorphic AFLP loci with as few as three AFLP primer pairs. This level of polymorphism is sufficient to assign paternity unambiguously to more than 99% of all seed in experiments involving small, known paternity pools. More generally, the AFLP procedure is well suited to molecular ecological studies, because it produces more polymorphism than allozymes or RAPDs but, unlike conventionally developed microsatellite loci, it requires no prior sequence knowledge and minimal development time.


1998 ◽  
Vol 88 (6) ◽  
pp. 520-524 ◽  
Author(s):  
José Luis Alonso-Prados ◽  
Miguel A. Aranda ◽  
José M. Malpica ◽  
Fernando García-Arenal ◽  
Aurora Fraile

Three hundred thirty-eight isolates of cucumber mosaic cucumovirus (CMV), sampled from natural populations in six areas of Spain between 1989 and 1996, were screened for the presence of satellite RNA (satRNA). The frequency of CMV isolates with satRNA approached 1.00 in Valencia (east Spain) between 1990 and 1994 where a tomato necrosis epidemic induced by CMV+satRNA had started in 1986 and was smaller north and west of this area in 1992 and 1993. After 1994, satRNA almost disappeared from all CMV populations. Genetic typing of satRNA variantswas done by ribonuclease protection assay, and from these data, genetic distances were estimated for any pair of satRNA variants. CMV-satRNA populations were highly diverse, containing 0.07865 nucleotide substitutions per site on average. Data also showed that the whole compared set of 100 satRNA variants form a single population that is not structured according to place, year, host plant, or strain of helper virus (HV). This is in sharp contrast with the metapopulation structure of the Spanish CMV population. Thus, the genetic structure and dynamics of populations of CMV and its satRNA are not coupled. This shows that CMV-satRNA spreads epidemically, as a hyperparasite, in the population of its HV. This conclusion is relevant to the use of CMV-satRNA as a biocontrol agent of CMV.


2012 ◽  
Vol 279 (1747) ◽  
pp. 4551-4558 ◽  
Author(s):  
William E. Bradshaw ◽  
Kevin J. Emerson ◽  
Julian M. Catchen ◽  
William A. Cresko ◽  
Christina M. Holzapfel

Identifying regions of the genome contributing to phenotypic evolution often involves genetic mapping of quantitative traits. The focus then turns to identifying regions of ‘major’ effect, overlooking the observation that traits of ecological or evolutionary relevance usually involve many genes whose individual effects are small but whose cumulative effect is large. Herein, we use the power of fully interfertile natural populations of a single species of mosquito to develop three quantitative trait loci (QTL) maps: one between two post-glacially diverged populations and two between a more ancient and a post-glacial population. All demonstrate that photoperiodic response is genetically a highly complex trait. Furthermore, we show that marker regressions identify apparently ‘non-significant’ regions of the genome not identified by composite interval mapping, that the perception of the genetic basis of adaptive evolution is crucially dependent upon genetic background and that the genetic basis for adaptive evolution of photoperiodic response is highly variable within contemporary populations as well as between anciently diverged populations.


Aquaculture ◽  
2017 ◽  
Vol 479 ◽  
pp. 91-99 ◽  
Author(s):  
Jonathan S.F. Lee ◽  
Matthew A. Cook ◽  
J. Adam Luckenbach ◽  
Barry A. Berejikian ◽  
Crystal A. Simchick ◽  
...  

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