EFFECT OF OXYGEN DEFICIENCY ON RADIATION-INDUCED MITOTIC DAMAGE IN SYNCHRONOUSLY DIVIDING CELLS

1959 ◽  
Vol 37 (6) ◽  
pp. 1061-1066 ◽  
Author(s):  
W. F. Baldwin ◽  
T. N. Salthouse

The latent effects of X irradiation in delaying mitosis are readily observable in the epidermis of the insect Rhodnius owing to the degree of synchrony of division in these cells following a blood meal. At the dose employed in these studies, mitosis did not proceed beyond metaphase when the insects were exposed in air; after irradiation at the same dose in nitrogen, a prolonged division was completed with the greater part of the inhibition occurring during metaphase.

1999 ◽  
Vol 152 (2) ◽  
pp. 144 ◽  
Author(s):  
Annamarie D. Horan ◽  
Albert R. Giandomenico ◽  
Cameron J. Koch

2002 ◽  
Vol 80 (7) ◽  
pp. 618-624 ◽  
Author(s):  
P Jacquet ◽  
J Buset ◽  
J Vankerkom ◽  
S Baatout ◽  
L de Saint-Georges ◽  
...  

PCC (premature chromosome condensation) can be used for visualizing and scoring damage induced by radiation in the chromatin of cells undergoing a G1 or G2 arrest. A method involving the fusion of irradiated single embryonic cells with single MI oocytes was used to induce PCC in mouse zygotes of the BALB/c strain, which suffer a drastic G2 arrest after X-irradiation (dose used 2.5 Gy). Other G2-arrested embryos were exposed in vitro to the phosphatase inhibitor calyculin A. Both methods furnished excellent chromosome preparations of the G2-arrested embryos. The mean number of chromosome fragments did not change significantly during G2 arrest, suggesting that zygotes of this strain are unable to repair DNA damage leading to such aberrations. Forty to fifty percent of the irradiated embryos were unable to cleave after G2 arrest and remained blocked at the one-cell stage for a few days before dying. PCC preparations obtained from such embryos suggested that about 30% of them had undergone a late mitosis not followed by cytokinesis and had entered a new DNA synthesis. These results are discussed in the light of recent observations in irradiated human cells deficient in the p53/14-3-3sigma pathway.Key words: PCC, embryo, oocyte, calyculin A, G2 arrest, cytokinesis.


1999 ◽  
Vol 31 (1-6) ◽  
pp. 591-594 ◽  
Author(s):  
M. Fujii ◽  
Y. Hatano ◽  
A. Osawa ◽  
T. Saito ◽  
K. Yamamoto ◽  
...  

1956 ◽  
Vol 52 (0) ◽  
pp. 311-320 ◽  
Author(s):  
P. J. Berry ◽  
S. Lipsky ◽  
M. Burton

1991 ◽  
pp. 391-394 ◽  
Author(s):  
Yoko Suyama ◽  
Mamoru Matsumoto ◽  
Sachiko Kageyama ◽  
Isao Sato

1968 ◽  
Vol 23 (6) ◽  
pp. 771-777 ◽  
Author(s):  
G. Lassmann ◽  
W. Damerau ◽  
Kh. Lohs

Chlorine containing phosphonic acid esters (tridilorphon-type) have been investigated quantitatively by ESR after X-irradiation at room temperature. Radical yields are between 0.2 and 1.7 radicals per 100 eV. In the case of trichlorphon the radical concentration is 7.5·1018 spins per gram at 40 Mrad.Stabilisation and kinetics of radiation-induced radicals in the solid state matrix at high irradiation doses in dependence of storage time, oxygen-effect and temperature are discussed. The obtained results suggest a model for the behaviour of the matrix at high irradiation doses. According to this model the lattice of molecular crystals is modified by the accumulation of recombination products arising from the induced radicals. These lattice imperfections cause a diminution of stabilizing properties for the radicals. The decrease of the dose-yield-curve at higher doses than 40 Mrad and the dose-dependent radical kinetics during storage can be explained by this model.


2019 ◽  
Vol 11 (4) ◽  
pp. 4514-4522 ◽  
Author(s):  
Ryan T. Frederick ◽  
J. Trey Diulus ◽  
Danielle C. Hutchison ◽  
May Nyman ◽  
Gregory S. Herman

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