Temporal expression of extracellular matrix metalloproteinases and tissue plasminogen activator in the development of collateral vessels in the canine model of coronary occlusion

1996 ◽  
Vol 74 (8) ◽  
pp. 983-995 ◽  
Author(s):  
S C Tyagi ◽  
S Kumar ◽  
S Cassatt ◽  
J L Parker
Keyword(s):  
Extracellular Matrix ◽  
Matrix Metalloproteinases ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Coronary Occlusion ◽  
Canine Model ◽  
Temporal Expression ◽  
Tissue Plasminogen ◽  
Collateral Vessels

scholarly journals Effect of intrafollicular indomethacin injection on gonadotropin surge-induced expression of select extracellular matrix degrading enzymes and their inhibitors in bovine preovulatory follicles

Reproduction ◽  
2006 ◽  
Vol 131 (3) ◽  
pp. 533-543 ◽  
Author(s):  
Qinglei Li ◽  
Fermin Jimenez-Krassel ◽  
Yasuhiro Kobayashi ◽  
James J Ireland ◽  
George W Smith
Keyword(s):  
Extracellular Matrix ◽  
Plasminogen Activator ◽  
Tissue Plasminogen Activator ◽  
Follicular Fluid ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Tissue Inhibitor ◽  
Preovulatory Follicles ◽  
Tissue Plasminogen ◽  
Gonadotropin Surge ◽  
Matrix Degrading Enzymes

A growing body of evidence supports an obligatory role for intrafollicular prostanoids in the mechanism of ovulation. However, the prostanoid-dependent mediators of the follicular extracellular matrix degradation required for ovulation are unknown. The objectives of this study were to determine the cellular compartment(s) in which the gonadotropin surge-induced regulation of select extracellular matrix degrading enzymes and their cognate inhibitors occurs in bovine preovulatory follicles, and to test whether such regulation is blocked by intrafollicular administration of the prostanoid synthesis and ovulation inhibitor, indomethacin (INDO). Follicular fluid prostaglandin E2concentrations were elevated in diluent-treated follicles before ovulation (24 h after GnRH injection), but the increase was blocked in INDO-treated follicles. Real-time PCR analysis revealed the specific follicular cell types where gonadotropin surge-induced increases in mRNA abundance for members of the matrix metalloproteinase/tissue inhibitor of metalloproteinase and plasminogen activator families occurred. INDO treatment increased thecal cell mRNA for tissue inhibitor of metalloproteinase-4 and its protein abundance in the apex of preovulatory follicles before ovulation, but suppressed granulosal cell mRNA and activity for tissue plasminogen activator in follicular fluid and the follicle apex. Plasmin activity was also suppressed in the follicular fluid of INDO-treated follicles. Effects of INDO injection on select matrix metalloproteinases were not observed. The results suggest that gonadotropin surge-induced regulation of tissue inhibitor of metalloproteinase-4 and tissue plasminogen activator may be prostanoid dependent, and support a potential role for increased tissue plasminogen activator expression and decreased tissue inhibitor of metalloproteinase-4 expression in the mechanism of ovulation.

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