Effects of nutrient(HCO3-)and CI- on potential defference responses to nutrient Na+ changes in secreting and inhibited states of frog stomach

1996 ◽  
Vol 74 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Manuel Schwartz ◽  
Theodore P Schilb ◽  
Gaspar Carrasquer
Keyword(s):  
Frog Stomach

Helicobacter pyloricytotoxin VacA increases alkaline secretion in gastric epithelial cells

2001 ◽  
Vol 281 (6) ◽  
pp. G1440-G1448 ◽  
Author(s):  
Lucantonio Debellis ◽  
Emanuele Papini ◽  
Rosa Caroppo ◽  
Cesare Montecucco ◽  
Silvana Curci
Keyword(s):  
Apical Membrane ◽  
Acid Output ◽  
Human Infection ◽  
Gastric Epithelium ◽  
Secondary Development ◽  
Acute Effects ◽  
Gastric Diseases ◽  
Gastric Glands ◽  
Frog Stomach ◽  
Alkaline Secretion

Human infection by the bacterium Helicobacter pylori (Hp) may lead to severe gastric diseases by an ill-understood process involving several virulence factors. Among these, the cytotoxin VacA is associated with higher tissue damage. In this study, the isolated frog stomach model was used to characterize the acute effects of VacA on the gastric epithelium. Our results show that VacA partially inhibits gastric acid output by increasing HCO[Formula: see text] efflux. Experiments conducted with double-barrelled pH or Cl−-selective microelectrodes on surface epithelial gastric cells (SECs) and single gastric glands show that VacA does not impair the activity of the oxyntic cells but renders the apical membrane of SECs more permeable to HCO[Formula: see text] and Cl−. Inhibition of this permeation by 5-nitro-2-(3-phenylpropylamino) benzoic acid indicates that this may be due to the formation of anion-selective pores by the toxin. We suggest that VacA-dependent HCO[Formula: see text] efflux from SECs improves the environmental conditions (pH, CO2concentration) of the niche parasitized by Hp, that is the gastric surface. This may favor Hp persistence in the tissue and the secondary development of a chronic inflammation.

scholarly journals Penggunaan sedasi inhalasi N 2 O-O 2 pada penatalaksanaan marsupialisasi ranula rongga mulut anak anxiety patient

2008 ◽  
Vol 7 (2) ◽  
pp. 78
Author(s):  
Harun Achmad ◽  
Dini Safitri ◽  
Kirana Lina Gunawan
Keyword(s):  
Dental Care ◽  
Side Effect ◽  
Dental Treatment ◽  
Minimal Side Effect ◽  
Inhalation Sedation ◽  
Anxiety Patient ◽  
Frog Stomach ◽  
Short Time ◽  
Anxiety And Fear ◽  
Selection Of

Inhalation sedation becomes the main choice in the dental treatment of children whoovercome have any kind of the fearness, concerned with pain in the treatment of theirteeth. Inhalation sedation with N2O-Ocauses sedation situation that accompanies byanalgesia to the conscious patients by inhaling the mixture of oxide nitrogen (N2O)with oxygen (O), that was known as psychosedation or concious sedation technique.Ranula is a mucocel that developed at the base of the mouth, unilateral, bluish, andthe shape like frog stomach. On this paper, will be esplained about N2O-O2inhalationsedation technique that concider the condition for the patient, using short time andcan be recovered immediately. A 9 year old girl diagnosed by ranula sublingualis onthe cavity of the mouth was very anxiety and fear in this treatment. The ranula treatedwith marsupialitation and was supervised by inhalation sedation of N2O-O2 in theSpecial Dental Care Hasan Sadikin Hospital Bandung. The result showed thatinhalation sedation with N2O-O2 was very very useful to reduce anxiety and fear tocertain patients with minimal side-effect. So it was very important to a dentist to knowthe selection of the patient, equipment and the technique of inhalation sedationadequately

Sites of resistance changes with inhibition of acid secretion in frog stomach

1986 ◽  
Vol 250 (5) ◽  
pp. G639-G647
Author(s):  
W. S. Rehm ◽  
G. Carrasquer ◽  
M. Schwartz
Keyword(s):  
Acid Secretion ◽  
Proton Pump ◽  
Tubular Cell ◽  
Secretory Rate ◽  
Tubular Cells ◽  
Resistance Increase ◽  
Frog Stomach ◽  
Scn Resistance ◽  
Gastric Proton Pump ◽  
Resistance Decrease

The primary purpose of this study was to determine the sites of resistance changes from thiocyanate inhibition of secretion to better evaluate models of the gastric proton pump. The potential difference, resistance, and H+ secretory rate were measured with various tonicities of the secretory fluid. Thiocyanate (SCN) inhibition generally produces a resistance increase, but with nutrient Ba, the resistance is high and SCN decreases it. The latter effect is also seen with cimetidine. In the secreting fundus a hypotonic secretory solution has a small effect on resistance, but in the inhibited fundus the effect is huge, due to increase of resistance of the lumen-tubular cell pathway. With a hypotonic secretory solution, for inhibited fundus (cimetidine or omeprazole) and antrum, SCN does not decrease the resistance of the surface cells and/or the transintercellular pathways with or without Ba. The SCN resistance decrease with Ba is via the tubular cell pathway. With Ba the resistance of the nutrient membrane of the tubular cells decreases after SCN or cimetidine inhibition. The findings further support the concept that under standard conditions the resistance via the lumina and tubular cells is low and that via the surface cells and transintercellular pathways is high.

Further Evidence of Na+ Conductance in Frog Stomach in Cl- -free Media

1979 ◽  
Vol 161 (3) ◽  
pp. 244-246 ◽  
Author(s):  
T. L. Holloman ◽  
M. Schwartz ◽  
G. Carrasquer
Keyword(s):  
Frog Stomach ◽  
Free Media

Evidence for a simple Cl− conductance pathway in nutrient membrane of frog stomach

1989 ◽  
Vol 980 (3) ◽  
pp. 367-370 ◽  
Author(s):  
Thomas L. Holloman ◽  
Manuel Schwartz ◽  
Gaspar Carrasquer ◽  
Warren S. Rehm ◽  
Mumtaz A. Dinno
Keyword(s):  
Frog Stomach ◽  
Cl Conductance

Role of rho-kinase (ROCK) in tonic but not phasic contraction in the frog stomach smooth muscle

Life Sciences ◽  
2018 ◽  
Vol 198 ◽  
pp. 46-55 ◽  
Author(s):  
Leyla Sahin ◽  
Ozge Selin Cevik ◽  
Dilan Deniz Koyuncu ◽  
Kansu Buyukafsar
Keyword(s):  
Smooth Muscle ◽  
Rho Kinase ◽  
Phasic Contraction ◽  
Frog Stomach

Effect of inhibitors and weak bases on electrophysiology and secretion in frog stomach.

1978 ◽  
Vol 234 (2) ◽  
pp. E120
Author(s):  
S S Sanders ◽  
J A Pirkle ◽  
R L Shoemaker ◽  
W S Rehm
Keyword(s):  
Weak Bases ◽  
Frog Stomach ◽  
Effect Of Inhibitors

Anomalous potential difference responses to changes in sodium concentration in the antrum of frog stomach

1983 ◽  
Vol 245 (4) ◽  
pp. G554-G561 ◽  
Author(s):  
G. Carrasquer ◽  
D. Kissel ◽  
W. S. Rehm ◽  
M. Schwartz
Keyword(s):  
Rana Catesbeiana ◽  
Apical Membrane ◽  
Sodium Concentration ◽  
Potential Difference ◽  
Conductive Pathway ◽  
Luminal Side ◽  
Frog Stomach ◽  
Conductance Ratio ◽  
Cl Conductance

Previously, an electrogenic Na-Cl symport was found in the fundus, and the question arises of whether there is one in the antrum, a tissue that does not secrete acid. In an in vitro preparation of the antrum of Rana catesbeiana, we found that when the [Na+] in nutrient solution was decreased (choline for Na+) the transmucosal potential difference (PD) decreased (the positivity of nutrient side decreased), and when the [Na+] was increased the PD increased. These PD changes were anomalous for Na+ but not for choline. A linear relationship for PD versus log [Na+] and not versus log [choline] excluded a choline conductive pathway. The anomalous PD response was decreased but not abolished by 10(-3) M ouabain. Normal PD responses resulted from [Na+] changes in Cl--free (SO2-4) solutions. PD responses to changes in nutrient [Cl-] were normal but decreased in the absence of Na+. Data are compatible with a passive electrogenic Na-Cl symport with more chloride than sodium ions transported per cycle, as in the fundus. Symport conductance-to-total Cl- conductance ratio is higher in antrum than in the fundus. Data from the luminal side were compatible with apical membrane conductances for Na+ and Cl-.

Effect of external sodium on net potassium uptake by frog muscle

1965 ◽  
Vol 208 (1) ◽  
pp. 61-67 ◽  
Author(s):  
W. McD. Armstrong
Keyword(s):  
Room Temperature ◽  
Ringer Solution ◽  
Frog Muscle ◽  
Potassium Uptake ◽  
Partial Replacement ◽  
Stomach Muscle ◽  
K Uptake ◽  
Frog Stomach ◽  
Frog Sartorius ◽  
Frog Stomach Muscle

When longitudinal strips of frog stomach muscle or frog sartorius muscles, previously depleted of K by overnight immersion in the cold in K-free Ringer solution containing 116 mEq Na/liter, were reimmersed at room temperature in solutions containing K, replacement of Na in the recovery fluid by Li or choline inhibited net K uptake by the fibers. Stomach muscles soaked overnight in Na-free solutions containing Li or choline lost K and most of their Na, the Na being replaced by Li and, inferentially, by choline. When these muscles were reimmersed in Na-Ringer solution they accumulated K from the medium. On reimmersion in Na-free Li or choline-Ringer solution no net K uptake occurred. Partial replacement of Li or choline by Na resulted in net K accumulation. Stomach muscles soaked in K-free Na-Ringer solution and subsequently transferred to Na-free solutions containing Cs and K did not accumulate K. Partial replacement of Cs by Na did not affect the inhibition of K uptake by Cs. K accumulation by frog stomach muscle was not affected by cyanide (2 mmoles/liter). These results are compatible with a specific stimulating effect of external Na on K accumulation in frog muscle.

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