Modulation of lipoprotein lipase activity in the rat by the β2-adrenergic agonist clenbuterol

1992 ◽  
Vol 70 (12) ◽  
pp. 1555-1562 ◽  
Author(s):  
Rekia Belahsen ◽  
Yves Deshaies
Keyword(s):  
Enzyme Activity ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Lipoprotein Lipase Activity ◽  
Serum Triglycerides ◽  
Postprandial State ◽  
Brown Adipose ◽  
Lateralis Muscle

This study evaluated the effects of β2-adrenoceptor stimulation on some determinants of triglyceride metabolism. Male Sprague–Dawley rats were injected twice daily with clenbuterol (30 μg∙kg−1) for 7 days, or with an equivalent volume of vehicle. Serum triglycerides, hepatic triglyceride secretion rate, and lipoprotein lipase activity in white and brown adipose tissues as well as in red vastus lateralis muscle and heart were evaluated in the fasting state and following a fat-free, high-sucrose meal, 3 h after the last agonist injection. In rats killed in the fasting and postprandial states, clenbuterol reduced the mass of white adipose tissue (−25 and −12%, respectively; p < 0.02), whereas it increased the mass of vastus lateralis muscle (+11 and +7%; p < 0.002) and heart (+13 and +10%; p < 0.0001). In vehicle-injected animals, the fasting state was associated with lower lipoprotein lipase activity in white and brown adipose tissues, and higher enzyme activity in vastus lateralis and heart, compared with the postprandial state. Postprandially, treatment with clenbuterol reduced lipoprotein lipase activity in white adipose (−24%), whereas it increased enzyme activity in brown adipose (+107%) as well as in vastus lateralis (+35%). In fasted animals, no significant variation of enzyme activity in these tissues was observed following clenbuterol treatment, whereas in the heart, a decrease of lipoprotein lipase activity was observed (−22%). Clenbuterol lowered serum triglycerides significantly (−23%), but not their rate of secretion, whereas the agonist decreased the insulin to glucagon ratio only in the postprandial state. These results suggest that modulation of triglyceride-rich lipoprotein metabolism, particularly tissue-specific alterations in lipoprotein lipase activity, may be one of the pathways through which clenbuterol affects partitioning of lipid substrates.Key words: triglyceride, lipoprotein lipase, tissue specificity, meal intake, β2 agonist.

Restoration of lipoprotein lipase activity in insulin-deficient rats by insulin infusion is tissue-specific

1991 ◽  
Vol 69 (6) ◽  
pp. 746-751 ◽  
Author(s):  
Yves Deshaies ◽  
Alain Géloën ◽  
Anne Paulin ◽  
Ludwik J. Bukowiecki
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Insulin Infusion ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Lipoprotein Lipase Activity ◽  
Adipose Tissues ◽  
Brown Adipose ◽  
Lateralis Muscle

The activity of lipoprotein lipase was measured in white and brown adipose tissues, red vastus lateralis muscle, and heart of rats that have been insulin deficient (streptozotocin, 75 mg∙kg−1) for 2 weeks, and that have then received implants of insulin-delivering minipumps (17 U∙kg−1∙day−1) for 1 or 4 days. Normal glycemia was restored in insulin-deficient animals after 4 days of insulin treatment. Hypertriglyceridemia, but not hypercholesterolemia, was reversed after 4 days of insulin infusion. After 2 weeks of insulin deficiency, fasting lipoprotein lipase activity was lowered in all tissues studied. In white adipose tissue, lipoprotein lipase decreased to 50% of control values. After a single day of insulin infusion, even if tissue weight has not yet been greatly affected, total activity was completely restored to control levels. Enzyme activity in brown adipose tissue was also depressed in deficient animals, and insulin infusion was followed by a slow recovery of activity, to a level intermediate between those of control and insulin-deficient groups. Insulin status had milder effects on lipoprotein lipase activity in vastus lateralis muscle than in the adipose tissues. Deficient rats displayed 60% less activity than controls, and 4 days of hormone infusion only partially restored enzyme activity. There was a large loss of lipoprotein lipase in the heart following 2 weeks of insulin depletion, which was not counteracted by hormone infusion. Thus the speed and extent of recovery of lipoprotein lipase activity following hormone replacement in insulin-deficient animals varied widely among tissues. These findings suggest that insulin is part of the factors that determine the tissue specificity of lipoprotein lipase regulation.Key words: insulin, lipoprotein lipase, white adipose tissue, brown adipose tissue, skeletal muscle, heart.

Refeeding and insulin increase lipoprotein lipase activity in rat brown adipose tissue

1989 ◽  
Vol 256 (5) ◽  
pp. E645-E650 ◽  
Author(s):  
C. M. Carneheim ◽  
S. E. Alexson
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Starvation Period ◽  
Lipoprotein Lipase Activity ◽  
Mrna Synthesis ◽  
Beta Adrenergic ◽  
Brown Adipose

Induction of lipoprotein lipase activity in brown adipose tissue (BAT) in response to cold stress has earlier been shown to be regulated by a beta-adrenergic mechanism and to be dependent on mRNA synthesis. In the present study, we have investigated the acute effects of refeeding after a short starvation period and the hormonal mechanism underlying the observed effects. Refeeding was found to rapidly increase tissue wet weight and lipoprotein lipase activity. The increase in enzyme activity could be blocked by the RNA synthesis inhibitor actinomycin D, indicating a gene activation. beta-Adrenergic blockade had no effect on this elevation of enzyme activity, but the increase could be mimicked by insulin injection. The results suggest that BAT contains two different pathways for regulation of lipoprotein lipase activity, both involving mRNA synthesis.

Muscle lipoprotein lipase activity in voluntarily exercising rats

1986 ◽  
Vol 60 (5) ◽  
pp. 1623-1627 ◽  
Author(s):  
G. J. Bagby ◽  
J. L. Johnson ◽  
B. W. Bennett ◽  
R. E. Shepherd
Keyword(s):  
Exercise Training ◽  
Succinate Dehydrogenase ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Vastus Lateralis ◽  
Voluntary Exercise ◽  
Vastus Lateralis Muscle ◽  
Mitochondrial Enzymes ◽  
Lipoprotein Lipase Activity ◽  
Voluntary Activity

Voluntary exercise of rats in freely rotating work wheels has been extensively used, but muscle adaptations that result from such exercise training are poorly documented. The purpose of this study was to determine whether the exercise performed by voluntarily active rats would increase succinate dehydrogenase or lipoprotein lipase activities in the soleus muscle (SM) or the red portion of the vastus lateralis muscle (RV). In SM the activities of these two enzymes were not increased after 7 or 16 wk of voluntary exercise. Succinate dehydrogenase activity in RV was moderately increased after 7 and 16 wk of voluntary activity (P less than 0.05). Substantial increases occurred in RV lipoprotein lipase activity (P less than 0.01). The increase in RV lipoprotein lipase activity was positively related to distance run by the rats. The results indicate that only small muscle-dependent increases in mitochondrial enzymes occur in rats allowed to exercise voluntarily in rodent work wheels. Voluntary exercise training induced a selective increase in lipoprotein lipase activity in a muscle containing a high percentage of fast-twitch red fibers, a response absent in a muscle containing a predominance of slow-twitch red fibers. It is unlikely that this differential response can be explained by exercise-induced changes in plasma hormone concentrations involved in the regulation of lipoprotein lipase.

Regulation of lipoprotein lipase in adipose and muscle tissues during fasting

1991 ◽  
Vol 260 (5) ◽  
pp. R953-R959 ◽  
Author(s):  
M. J. Ladu ◽  
H. Kapsas ◽  
W. K. Palmer
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Vastus Lateralis ◽  
Vastus Lateralis Muscle ◽  
Mrna Concentration ◽  
Muscle Tissues ◽  
Lateralis Muscle ◽  
Muscle Changes ◽  
Fast Twitch

The purpose of this work was to determine the relationship between lipoprotein lipase (LPL) activity and LPL mRNA in muscle and adipose tissue in fed and fasted rats. In control animals, the correlation between enzyme activity and LPL mRNA for adipose tissue, heart, soleus, fast red vastus lateralis, and fast white vastus lateralis muscle was r = +0.97. Twenty-four hours of fasting increased LPL activity 38% in heart, reduced it 59% in adipose tissue, and had no effect on activity in the three skeletal muscles analyzed. At the same time, relative LPL mRNA concentrations were reduced 25% in adipose tissue and elevated in heart, soleus, red vastus, and white vastus muscles when compared with control concentrations. Prolonging the fast to 6 days was accompanied by a 64% reduction in adipose tissue LPL activity and an increase in the activities of slow-twitch soleus (83%) and fast-twitch red vastus lateralis muscles (193%), with no enzyme activity change in heart or white vastus lateralis muscle compared with values obtained from control fed animals. LPL mRNA concentration was reduced 66% in adipose tissue, increased more than twofold in heart, soleus, and white vastus muscle, and increased threefold in red vastus muscle. Changes in relative LPL mRNA concentration in adipose tissue induced by fasting could, in part, be accounted for by the increases seen in total RNA concentration. The relationships between enzyme activity and LPL mRNA in muscle and adipose tissue were r = 0.97 and 0.77 for 1-day and 6-day fasted animals, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals The Effect of Melinjo Peel Extract in Activity of Lipoprotein Lipase Enzym of The Rats Fed a Hypercholesterolemia Diet

2018 ◽  
Vol 7 (2) ◽  
pp. 133
Author(s):  
Athira Demitri
Keyword(s):  
Enzyme Activity ◽  
Statistical Analysis ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Diet Group ◽  
Lipoprotein Lipase Activity ◽  
Oral Gavage ◽  
Hypercholesterolemic Diet ◽  
Before And After ◽  
Peel Extract

The intake of saturated fat and cholesterol that comes from food digested in the intestine resulting in free fatty acids, triglycerides, phospholipids, and cholesterol. Triglyceride levels increased can be caused by presence of impaired lipoprotein lipase (LPL) enzyme activity. LPL activity can be increased by flavonoid in plant, like Melinjo peel. The purpose of this study is to measure LPL enzyme activity before and after being given extract of Melinjo peel treatment. This research use true experimental, with study design of pretest posttest control group design. Hypercholesterolemic diet given to rats by oral gavage as much as 1,8 grams for 14 days. Melinjo peel extract were given by oral gavage for 14 days after the hypercholesterolemic diet is given. Statistical analysis used Paired Sample T-Test to compare lipoprotein lipase activity before and after treatment. Then, used MANOVA (Multivariate Analyses of Variance) to see the difference of lipoprotein lipase activity in each group after treatment.Based on statistical analysis showed that there were differences of lipoprotein lipase activity in the hypercholesterolemic diet group, HD+54.15, HD+108.30, and HD+216.60 before and after treatment significant (p. < 0.05). The activity of lipoprotein lipase in the normal diet group compared with the hypercholesterolemic and HD+216.60 group showed a significant difference (p. < 0.05). Melinjo peel extract can increased activity of lipoprotein lipase enzym after treatment, those can be due to the flavonoid in Melinjo peel extract.

Plasma Lipoprotein Lipase in Children with Idiopathic Nephrotic Syndrome

PEDIATRICS ◽  
1969 ◽  
Vol 44 (6) ◽  
pp. 1021-1024
Author(s):  
Lawrence R. Hyman ◽  
Paul W. K. Wong ◽  
Aaron Grossman
Keyword(s):  
Enzyme Activity ◽  
Nephrotic Syndrome ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Idiopathic Nephrotic Syndrome ◽  
Plasma Lipoprotein ◽  
Lipoprotein Lipase Activity ◽  
The Mean

Plasma lipoprotein lipase activity was determined in six children with idiopathic nephrotic syndrome and in six controls. The mean lipoprotein lipase activity was significantly lower in the symptomatic nephrotic patients. This decreased enzyme activity may contribute to the hyperlipemia usually observed in the nephrotic syndrome. See table in the PDF file

scholarly journals Changes in the lipoprotein lipase (clearing-factor lipase) activity of white adipose tissue during development of the rat

1978 ◽  
Vol 172 (2) ◽  
pp. 319-325 ◽  
Author(s):  
A Cryer ◽  
H M Jones
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Plasma Insulin ◽  
Serum Concentrations ◽  
Lipoprotein Lipase Activity ◽  
Activity Change ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase (clearing-factor lipase) activity of the white adipose tissue from rats aged between 1 and 145 days was determined. Five adipose-tissue sites (epididymal, uterine, subcutaneous, perirenal and intramuscular) together with serum concentrations of triacylglycerol, cholesterol and glucose were studied. The pattern of enzyme-activity change was remarkably similar in all the sites studied, although the growth of the tissues proceeded non-uniformly. After a peak of activity early in suckling, lipoprotein lipase activity fell to low values by 20 days of age. At weaning (21 days) the activity increased sharply and within 5 days high values were regained. The serum triacylglycerol and cholesterol concentrations were low at birth and reached peaks of concentration coincidentally with the minima of white-adipose-tissue lipoprotein lipase activities, seen late in suckling. The changes in enzyme activity were related to other metabolic changes in adipose tissue and with the known changes in plasma insulin concentrations occurring during development.

scholarly journals Alteration in lipoprotein lipase activity bound to triglyceride-rich lipoproteins in the postprandial state in type 2 diabetes

2004 ◽  
Vol 45 (5) ◽  
pp. 859-865 ◽  
Author(s):  
Valérie Pruneta-Deloche ◽  
Agnès Sassolas ◽  
Geesje M. Dallinga-Thie ◽  
François Berthezène ◽  
Gabriel Ponsin ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Lipoprotein Lipase ◽  
Lipase Activity ◽  
Lipoprotein Lipase Activity ◽  
Postprandial State
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