Lipoprotein lipase activity in white adipose tissue of rats subjected to exercise–rest cycles

1990 ◽  
Vol 68 (2) ◽  
pp. 157-163 ◽  
Author(s):  
Yves Deshaies ◽  
Gilles Lortie ◽  
Denis Richard
Keyword(s):  
Adipose Tissue ◽  
Exercise Training ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Body Weight Gain ◽  
Density Lipoprotein ◽  
Treadmill Running ◽  
Lipoprotein Lipase Activity ◽  
Intermittent Training

This study was conducted to determine serum lipid levels and the activity of lipoprotein lipase in epididymal white adipose tissue of rats undergoing exercise training. During the 8-week period of treatment, one group of rats was kept sedentary and the remaining animals were exercise trained either continually (1 h of daily treadmill running) or intermittently (alternate weeks of daily running and inactivity). Exercise training, either continual or intermittent, decreased postprandial serum total and high-density lipoprotein cholesterol concentrations, which returned to sedentary levels in the intermittently trained animals following a week of rest. Lipoprotein lipase activity in whole epididymal adipose pad was lower in rats trained continually than in the sedentary group at the end of the treatment. The intermittent training program elicited large fluctuations in both the specific (per milligram of protein) and total (per tissue) activity of lipoprotein lipase in white adipose tissue. During rest periods, enzyme activity rose to levels that were higher than those of sedentary rats, whereas lipase activity was below that of sedentary animals following a week of running. In the last exercise–rest cycle, body weight gain of the intermittently trained rats was nearly abolished during the week of running, but it increased above that of sedentary animals during weeks of rest. The present results suggest that the modulation of lipoprotein lipase activity in white adipose tissue is one of the adaptations that take place to accommodate the fluctuations in the rate of energy deposition that occur in the rat during an intermittent training program.Key words: exercise training, cholesterol, white adipose tissue, lipoprotein lipase.

Plasma lipoprotein cholesterol and triglycerides and lipoprotein lipase activity in epididymal white adipose tissue of rats fed high sucrose or high corn oil diets

1986 ◽  
Vol 64 (7) ◽  
pp. 885-891 ◽  
Author(s):  
Yves Deshaies
Keyword(s):  
Adipose Tissue ◽  
Total Cholesterol ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Corn Oil ◽  
Reference Group ◽  
Density Lipoprotein ◽  
Lipoprotein Lipase Activity ◽  
Adipose Tissue Lipoprotein Lipase

The present study was undertaken to compare plasma lipoprotein lipid composition, as well as white adipose tissue lipoprotein lipase activity, in rats fed purified diets high in either sucrose or corn oil. The experimental diets (65% of calories as sucrose or corn oil, 15% as the opposite nutrient, and 20% as casein) were given ad libitum for 4 weeks. An additional group was fed a nonpurified diet as a reference diet. Both sucrose and oil diets were spontaneously consumed in isocaloric amounts by the animals. Despite energy intakes that were 35% lower than that of the reference group, the sucrose and oil groups exhibited final body weights that were only 6 and 9% lower, respectively, than that of the reference group, and accumulated more fat in the epididymal depots. Postprandial as well as fasting total cholesterol levels were similar in the sucrose and oil groups, while the high-density lipoprotein to total cholesterol ratio was highest in the animals fed corn oil. In both the fasted and fed states, plasma total triglyceride levels were 73% higher in the sucrose group than in the corn oil group. The largest triglyceride differences due to diet were observed in the chylomicron + very-low-density lipoprotein fraction. The oil-fed rats accumulated large amounts of triglycerides in their livers. Postprandial lipoprotein lipase activity in epididymal adipose tissue was almost twice as high in the sucrose group as in the oil group. In the fasting state, but not in the fed state, plasma insulin levels correlated negatively with plasma triglycerides and positively with liver triglyceride content, whereas no relationship was observed between insulin and lipoprotein lipase activity in either fasted or fed animals. From the present findings, it appears that the sucrose-induced hypertriglyceridemia was not partly caused by decreased activity of lipoprotein lipase in white adipose tissue. Finally, this study suggests that the level of circulating insulin following intake of sucrose or corn oil may not determine per se the extent of stimulation of white adipose tissue lipoprotein lipase by these nutrients.

scholarly journals Effects of tri-iodothyronine administration on the disposal of oral [1-14C]triolein, lipoprotein lipase activity and lipogenesis in the rat during lactation and on removal of the litter

1994 ◽  
Vol 301 (2) ◽  
pp. 495-501 ◽  
Author(s):  
M Del Prado ◽  
T H Da Costa ◽  
D H Williamson
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Mammary Gland ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Plasma Prolactin ◽  
Lipoprotein Lipase Activity ◽  
14Co2 Production ◽  
Pup Growth

The effect of tri-iodothyronine (T3) administration on the utilization of dietary [14C]lipid by the mammary gland and adipose tissue of lactating and litter-removed rats was studied. (1) After an oral load of [1-14C]triolein, the lactating rats treated with T3 (50 micrograms/100 g body wt.) over 24 h showed an increase in 14CO2 production and a decrease in the total [14C]lipid transferred through the mammary gland that was paralleled by a decrease in tissue lipoprotein lipase (LPL) activity. (2) T3 administration decreased plasma prolactin in the lactating rats. Prolactin replacement in T3-treated rats restored LPL activity in the mammary gland, but did not increase the amount of dietary [14C]lipid transferred to the milk. (3) Chronic T3 administration (4 days) to lactating rats did not affect pup growth or the lipogenic rate in the mammary gland. (4) The administration of T3 to litter-removed rats inhibited the increase of LPL activity in white adipose tissue and decreased the accumulation of dietary [14C]lipid. This decrease was accompanied by increased 14CO2 production and [14C]lipid accumulation in skeletal muscle and heart. (5) It is concluded that hyperthyroidism depresses LPL activity in mammary gland and white adipose tissue, but not in muscle. The increased accumulation of [14C]lipid in muscle and increased production of 14CO2 in lactating and in litter-removed rats treated with T3 is in part due to the decreased total LPL in mammary gland and adipose tissue respectively, which are therefore less able to compete with muscle for the available plasma triacylglycerols.

scholarly journals Changes in the lipoprotein lipase (clearing-factor lipase) activity of white adipose tissue during development of the rat

1978 ◽  
Vol 172 (2) ◽  
pp. 319-325 ◽  
Author(s):  
A Cryer ◽  
H M Jones
Keyword(s):  
Enzyme Activity ◽  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
White Adipose Tissue ◽  
Lipase Activity ◽  
Plasma Insulin ◽  
Serum Concentrations ◽  
Lipoprotein Lipase Activity ◽  
Activity Change ◽  
Adipose Tissue Lipoprotein Lipase

The lipoprotein lipase (clearing-factor lipase) activity of the white adipose tissue from rats aged between 1 and 145 days was determined. Five adipose-tissue sites (epididymal, uterine, subcutaneous, perirenal and intramuscular) together with serum concentrations of triacylglycerol, cholesterol and glucose were studied. The pattern of enzyme-activity change was remarkably similar in all the sites studied, although the growth of the tissues proceeded non-uniformly. After a peak of activity early in suckling, lipoprotein lipase activity fell to low values by 20 days of age. At weaning (21 days) the activity increased sharply and within 5 days high values were regained. The serum triacylglycerol and cholesterol concentrations were low at birth and reached peaks of concentration coincidentally with the minima of white-adipose-tissue lipoprotein lipase activities, seen late in suckling. The changes in enzyme activity were related to other metabolic changes in adipose tissue and with the known changes in plasma insulin concentrations occurring during development.

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