Preservation of guinea pig hearts by hypothermic gas perfusion

1989 ◽  
Vol 67 (7) ◽  
pp. 692-696
Author(s):  
Leslie E. Bailey
Keyword(s):  
Guinea Pig ◽  
Storage Time ◽  
Contractile Activity ◽  
Myocardial Damage ◽  
Perfusate Flow ◽  
Transplant Center ◽  
Tissue Calcium ◽  
Oxygen Requirements ◽  
Long Term Preservation

The lack of a satisfactory method for long-term preservation of hearts during transport limits the source of human hearts for transplant to the geographic vicinity of the transplant center. Experimentally, reduction of myocardial oxygen requirements with hypothermia and cardioplegia prolong storage time to 48 h, but always with some evidence of myocardial damage. In this study, the combination of hypothermia with a procedure known to increase oxygen tension in cardiac muscle, gas perfusion, preserved contractile activity in guinea pig hearts for 24 h and did not cause edema. Cardioplegia or gas perfusion at temperatures below 10 °C or above 20 °C resulted in failure of hearts to contract upon rewarming. Contracture, dehydration, elevation of tissue calcium, reduced perfusate flow, and elevated creatine kinase levels occurred if liquid reperfusion was begun at 15 °C but not 25 °C. The results suggest that under the appropriate conditions, hypothermic gas perfusion is a potentially useful means of extending storage time of hearts for transplant.Key words: myocardial preservation, heart storage, hypothermia, gas perfusion, contractility.

scholarly journals Isolation and long term preservation of pancreatic islets from mouse, rat and guinea pig

Diabetologia ◽  
1976 ◽  
Vol 12 (2) ◽  
pp. 115-121 ◽  
Author(s):  
J. Ferguson ◽  
R. H. Allsopp ◽  
R. M. R. Taylor ◽  
I. D. A. Johnston
Keyword(s):  
Guinea Pig ◽  
Pancreatic Islets ◽  
Long Term Preservation

Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3-O-Gallate

2009 ◽  
Vol 18 (5-6) ◽  
pp. 513-520 ◽  
Author(s):  
Hakhee Kim ◽  
Takeshi Kawazoe ◽  
Kazuaki Matsumura ◽  
Shigehiko Suzuki ◽  
Suong-Hyu Hyon
Keyword(s):  
Storage Time ◽  
Radical Scavenging ◽  
Glucose Consumption ◽  
Skin Grafts ◽  
Dermal Matrix ◽  
Skin Sample ◽  
Biopsy Specimens ◽  
Previously Treated ◽  
Long Term Preservation

Skin grafts can be preserved by cryopreservation and refrigerated storage at 4°C. Epigallocatechin-3- O-gallate (EGCG) enhances the viability of stored skin grafts and also extends the storage time up to 7 weeks at 4°C. EGCG, the major polyphenolic constituent present in green tea, has potent antioxidant, antimicrobial, antiproliferative, and free radical scavenging effects. This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to −196°C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matrix. The present findings suggest the future clinical usefulness of EGCG for skin preservation; however, the mechanism by which EGCG promotes skin preservation still remains unclear.

scholarly journals Orchid cryopreservation

2014 ◽  
Vol 38 (3) ◽  
pp. 213-229 ◽  
Author(s):  
Wagner Vendrame ◽  
Ricardo Tadeu de Faria ◽  
Mauren Sorace ◽  
Sandra Aparecida Sahyun
Keyword(s):  
Plant Material ◽  
Storage Time ◽  
Natural Habitat ◽  
Orchid Species ◽  
Commercial Use ◽  
The Family ◽  
Relevant Field ◽  
Long Term Preservation ◽  
Intermediate Seeds

Orchids are lush and highly valuable plants due to their diversity and the beauty of their flowers, which increases their commercialization. The family Orchidaceae comprises approximately 35,000 species, distributed among more than 1,000 distinct genera and 100,000 hybrids, totaling approximately 8% to 10% of all flowering plants. With the advance of agriculture and the constant destruction of their natural habitat, orchid species are collected in an indiscriminate manner by collectors and vendors, and this extractive activity threatens many species with extinction, drastically reducing their genetic variability in nature. Therefore, it is essential to seek alternatives that make the preservation of such species feasible using techniques with low maintenance costs that provide greater storage time and that enable good phytosanitary conditions for the plant material for commercial use. Cryopreservation involves the conservation of biological materials at ultra-low temperatures, generally in liquid nitrogen at -196 ºC or in its vapor phase at -150 ºC. This is the only technique currently available for the long-term preservation of the germplasm of plant species that are vegetatively propagated or that have unviable, recalcitrant or intermediate seeds. The objective of this bibliographic review is to report on the importance, methods and application of cryopreservation for orchids. According to the studies reviewed, this is an incipient, developing and relevant field that generates a lot of discussion and requires further research relative to the type of treatment to use for cryopreservation and the methodology to be applied according to the species. The types of methods that are used for cryopreservation and the large variation in the responses of orchids to the cryopreservation methods observed in this study emphasize the need for the development of more appropriate protocols for the preservation of orchids.

Electrical, contractile, and ultrastructural properties of adult rat and guinea-pig ventricular myocytes in long-term primary cultures

1989 ◽  
Vol 67 (7) ◽  
pp. 740-750 ◽  
Author(s):  
M. Horackova ◽  
C. Mapplebeck
Keyword(s):  
Guinea Pig ◽  
Cultured Cells ◽  
Ventricular Myocytes ◽  
Contractile Activity ◽  
Primary Cultures ◽  
Morphological Characteristics ◽  
Ultrastructural Organization ◽  
Adult Rat ◽  
Intercellular Contacts

The electrical, contractile, and morphological characteristics of ventricular myocytes isolated from adult rat and guinea-pig hearts and maintained in cultures for 7–24 days are described. These cultured cells form different networks, depending on the species, when plated at certain density and maintained under specific conditions; the cells within the networks appear to be electrically coupled. Their resting and action potentials, their contractile activity (shortenings), and their pharmacological responses qualitatively resemble those of freshly isolated myocytes. Cultured cells from both species exhibit near-normal ultrastructural organization of sarcomeres, myofilaments, and mitochondria, as well as formation of intercellular contacts, or gap junctions. These data indicate that cultured adult rat and guinea-pig myocardial cells that make intercellular contacts possess electrical, contractile, and ultrastructural properties and responses to pharmacological agents similar to those of the respective adult myocardial tissues and the functionally intact freshly isolated cells from which these cultures are prepared. Thus, this study indicates that long-term cultures (7–24 days) of networked cardiac myocytes could be used as a valuable experimental model in various investigations of excitation–contraction coupling in cardiac muscle.Key words: cultured adult cardiomyocytes, contractility, electrical activity, ultrastructure, long-term primary cultures.

Problems of treatment of primary openangle glaucoma in combination with thrombosis of the central retinal vein

GlaucomaNews ◽  
2020 ◽  
pp. 65-69
Author(s):  
T.E. Lipatkina ◽  
◽  
Е.V. Karlova ◽  
A.V. Zolotarev ◽  
◽  
...  
Keyword(s):  
Macular Edema ◽  
Primary Open Angle Glaucoma ◽  
Antihypertensive Drugs ◽  
Open Angle Glaucoma ◽  
Vascular Wall ◽  
Open Angle ◽  
Retinal Edema ◽  
Long Term Preservation ◽  
Central Retinal Vein

Patients with primary open-angle glaucoma (POAG) and ophthalmic hypertension have an increased likelihood of developing occlusions (thrombosis) of the central retinal vein. Different groups of antihypertensive drugs differ in their mechanism of action and may affect concomitant ocular pathology, in particular, retinal edema, which occurs, for example, in occlusion of the central retinal vein. Used in most patients with glaucoma, prostaglandin analogs can contribute to the long-term preservation of macular edema due to the effect on the permeability of the vascular wall. Preparations of other pharmacological groups, reducing the production of aqueous humor, on the contrary, may contribute to its regression. Therefore, the question of choosing a drug for antihypertensive therapy in patients with primary open-angle glaucoma and concomitant macular edema is relevant and is for further study.

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