Effect of chronic sulfonylurea treatment on the myocardium of insulin-dependent diabetic rats

1988 ◽  
Vol 66 (12) ◽  
pp. 1481-1486 ◽  
Author(s):  
Mahmood S. Mozaffari ◽  
Glenn L. Wilson ◽  
Stephen W. Schaffer
Keyword(s):  
Glucose Utilization ◽  
Fasting Blood Glucose ◽  
Active Form ◽  
Lactate Production ◽  
Diabetic Rats ◽  
Diabetic Heart ◽  
Adult Rats ◽  
Glucose Levels ◽  
Insulin Responsiveness ◽  
Insulin Dependent Diabetic

Adult rats treated with high doses of streptozocin became progressively more hyperglycemic during the first month of the diabetic condition. Treatment of these rats with the sulfonylurea glyburide halted, and in some cases, reversed this process in a high percentage of the diabetics. Associated with the glyburide-mediated improvement in fasting blood glucose levels was an increase in myocardial glucose utilization and lactate production. The stimulation of myocardial glucose utilization by insulin was greater in glyburide-treated hearts, indicating that the hyperglycemic agent increased insulin responsiveness. The sulfonylurea also partially restored insulin sensitivity to the normal range. In agreement with previous studies, myocardial mechanical function was significantly impaired in the diabetic heart. When treated with glyburide, the severity of the mechanical defect was significantly less. The sulfonylurea also promoted an increase in myosin ATPase activity and a shift in the myosin isozyme pattern in favour of the most active V1 form. These results imply that glyburide therapy can provide benefit to the diabetic heart by improving energy metabolism and promoting a shift in myosin towards the most active form.

scholarly journals Assessment on Functionality and Viability of β cells Following Repetitive Dosage Administration of Ethanolic Extracts of Andrographis paniculata on Streptozotocin-induced Diabetic Rats

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Abdul Razak K ◽  
Mariam A ◽  
Amirin S ◽  
Mohd Zaini A
Keyword(s):  
Blood Glucose ◽  
Plant Extracts ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Andrographis Paniculata ◽  
Β Cells ◽  
Glucose Levels ◽  
Ethanolic Extracts ◽  
Before And After

Introduction: The study was done at the aim to assess the functionality and viability of the β cells of the streptozotocin-induced diabetic rats model following repetitive dosage of administration of ethanolic extracts of Andrographis paniculata. Materials and Methods: The diabetic rats were treated with the extracts for fourteen days and at the dose given was 500 mg/kg twice daily. The assessments were made on fasting blood glucose, insulin, and immunohistochemical aspect of β cells before and after treatment. Results: The results showed that there was a significant reduction on fasting blood glucose levels in metformin, 95% and 50% ethanolic plant extracts-treated groups but on insulin level only 95% and 50% ethanolic extracts-treated groups gave a significant reduction (p<0.05). Immunohistochemical assessments revealed that all extract groups and metformin-treated were significantly increased in the population of β cells (p<0.01). Conclusion: This study revealed that the plant extracts showed an ability to promote the growth or rejuvenate the STZdestructed β cells and in turn lower the blood glucose level.

scholarly journals Modulations of Some Carbohydrate Metabolic Enzymes by Aqueous and Ethanol Buchholzia coriacea Seed Extract in Alloxan Induced Diabetic Rats

2018 ◽  
pp. 1-9
Author(s):  
K. O. Gbagbeke ◽  
A. O. Naiho ◽  
P. R. C. Esegbue ◽  
M. O. Odigie ◽  
M. A. Omoirri
Keyword(s):  
Blood Glucose ◽  
Aqueous Extract ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Metabolic Enzymes ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Total Carbohydrate ◽  
Adult Rats ◽  
Ethanol Extracts

Characterised by abnormal increase in blood glucose level, Diabetes is a metabolic disorder that is associated with complications in carbohydrate, protein and fat metabolism. In recent times, medicinal herbs have been implicated in traditional medical practice for the treatment of this ailment. Studies have shown that Buchholzia coriacea seed possesses some anti-hyperglycemic properties that may be useful in the management of diabetes. To this point, present study investigated the effect(s) of oral administration of aqueous and ethanol extracts of Buchholzia coriacea on some carbohydrate metabolism parameters in normal and alloxan-induced diabetic rats. Forty (40) adult rats of both sexes were randomly assigned into two groups (normoglycemic and hyperglycemic). While group 1 (normoglycemic) had normal control, metformin, aqueous extract (250mg/kg) and ethanol extract (250 mg/kg) treated sub-groups respectively, Group 2 (hyperglycemic) contained the diabetic control, metformin, aqueous extract (250 mg/kg), and ethanol extracted (250mg/kg) treated sub-groups dosed daily by oral gavage for 14 days. At the end of the treatment, rats were euthanized via cervical dislocation; blood samples were collected by cardiac puncture for statistical analysis. One-way analysis of variance (ANOVA) revealed that dosing with extracts had insignificant effect(s) on body weight of rats. Fasting Blood Glucose (FBG) levels were elevated before and after extracts administration. Metformin, aqueous and ethanol extracts significantly reduced (p<0.05) FBG levels. Also, compared with control, total carbohydrate, liver glucose, glycosylated haemoglobin, Lactate Dehydrogenase, Isocitrate dehydrogenase, MDH, SDH, 6-phosphogluconate dehydrogenase, G6PD and CcO activities were significantly reduced (p<0.05) in diabetic treated rats. Buccholzia Coriacea was therefore seen to pose hypoglycemic and glycolytic effects, regulating activities of carbohydrate metabolic enzymes. Apparently, there is a scientific merit in the use of the extract in the management of diabetes.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Antidiabetic, Antihyperlipidemic and Renal Protective Activities of Garcinia indica Linn. Clusiaceae

2018 ◽  
Vol 10 (01) ◽  
Author(s):  
Amol Bhalchandra Deore ◽  
Vinayak Dnyandev Sapakal ◽  
Nilofer S. Naikwade
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Plasma Lipid ◽  
Ethanol Extract ◽  
Hdl Cholesterol ◽  
Diabetic Rats ◽  
Lipid Levels ◽  
Glucose Levels ◽  
Garcinia Indica ◽  
Blood Glucose Levels

To investigate the antidiabetic, antihyperlipidemic and renal protective activities of the aqueous and ethanol extract of Garcinia indica fruit rinds against alloxan induced diabetes in rats. Wistar rats were made diabetic by a single dose of alloxan hydrate [130 mg/kg i.p.]. After the successful induction of experimental diabetes, rats were divided into five groups each comprising a minimum of six rats. The effects of extracts and glibenclamide on fasting blood glucose, plasma lipid levels and renal profile were examined for 21 days. Blood glucose levels and biochemical parameters such as serum cholesterol, triglycerides, HDL-cholesterol, LDL-cholesterol, urea and creatinine levels of rats were measured using on weekly intervals i.e day 0, 7, 14 and 21 after daily administration of all extracts at dose of 500 mg/kg. Statistical analysis was performed using Dunnett’s test. p less than 0.01 was taken as the criterion of significance. Oral administration of both aqueous and ethanol extract for 21 days caused a significant [p less than 0.01] reduction in blood glucose levels, lipid profile except HDL; urea and creatinine in diabetic rats. Garcinia indica fruit rind possesses antihyperglycemic activity as well improves total lipid levels and renal profile. It can justify folklore uses of the plant in diabetes.

scholarly journals Comprehensive Evaluation of Anti-hyperglycemic Activity of FractionatedMomordica charantiaSeed Extract in Alloxan-Induced Diabetic Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-10 ◽  
Author(s):  
Shailesh Kumar Choudhary ◽  
Gagan Chhabra ◽  
Dipali Sharma ◽  
Aruna Vashishta ◽  
Sujata Ohri ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Comprehensive Evaluation ◽  
Fasting Blood Glucose ◽  
Protein S ◽  
Protein Band ◽  
Diabetic Rats ◽  
Momordica Charantia ◽  
Size Exclusion ◽  
Glucose Levels ◽  
Blood Glucose Levels

The present study evaluates anti-hyperglycemic activity of fractionatedMomordica charantia(bitter gourd) seed extracts. Fasting blood glucose levels were evaluated before and after administration of different fractions of the seed extract. Among the three fractions tested, fraction Mc-3 (15 mg/kg b.wt.) showed the maximum anti-hyperglycemic activity and reduced blood glucose levels in experimental diabetic rats significantly. The activities of the key regulatory enzymes of glucose metabolism (hexokinase, pyruvate kinase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase) were determined in Mc-3-treated diabetic animals. Once-daily administration of the fraction Mc-3 for prolonged period of 18 days to the experimental diabetic animals did not result in any nephrotoxicity or hepatotoxicity as evident from insignificant changes in biochemical parameters indicative of liver and kidney functions. Further fractionation of the fraction Mc-3 by size exclusion chromatography resulted in a fraction, designated Mc-3.2, possessing anti-hyperglycemic activity. The fraction Mc-3.2 showed the presence of a predominant protein band of ~11 kDa on SDS-PAGE. Loss in anti-hyperglycemic activity of the Mc-3.2 upon protease treatment indicates the proteinaceous nature of the anti-hyperglycemic principles. Overall, the results suggest thatMomordica charantiaseeds contain an effective anti-hyperglycemic protein(s) which may find application in treatment of diabetes without evident toxic effects.

scholarly journals Glycaemic, Lipidaemic and Antioxidant Profiles of Alloxan-Induced Diabetic Wistar Rats Treated with Glibenclamide and Aqueous Extract of Gongronema latifolium (Benth)

2016 ◽  
Vol 8 (4) ◽  
pp. 408-413
Author(s):  
Patrick Emeka ABA
Keyword(s):  
Wistar Rats ◽  
Combined Therapy ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Study Groups ◽  
Oral Route ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Male Wistar Rats

The current study investigated the ameliorative effects of combined therapy of glibenclamide and G. latifolium (GL) on several biochemical parameters of alloxaized Wistar rats. Thirty adult male Wistar rats assigned into 5 groups of 6 rats each were used for the study. Groups 2-5 were intraperitoneally injected with 160 mg/kg of alloxan monohydrate and upon establishment of diabetes (Fasting Blood Glucose (FBG) ≥ 126 mg/dl) were treated with 10 ml/kg distilled water (DW), 2 mg/kg glibenclamide, 200 mg/kg GL and 2 mg/kg glibenclamide and 200 mg/kg GL respectively. Rats in group 1 were not made diabetic and served as normal control. All the treatments were realized through daily oral route using gastric tube, for 21 days. Results indicated that the treatment of diabetic rats with a combination of glibenclamide and GL significantly reduced the elevated glucose levels, cholesterol, triacylglycerol, low density lipoprotein and malondialdehyde levels, along with increases in the high density lipoprotein, glutathione values and catalase activities, when compared to diabetic untreated group. It was concluded that the combined therapy of glibenclamide and GL showed superior antihyperglycemic, hypolipidaemic and antioxidant effects compared to either of them used alone.

scholarly journals Evaluation ofMallotus oppositifoliusMethanol Leaf Extract on the Glycaemia and Lipid Peroxidation in Alloxan-Induced Diabetic Rats: A Preliminary Study

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
C. O. Nwaehujor ◽  
I. I. Ezeigbo ◽  
F. C. Nwinyi
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Activity ◽  
Blood Glucose ◽  
Oral Treatment ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Albino Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Objective. Mallotus oppositifolius(Geiseler) Müll. Arg. (Euphorbiaceae) is folklorically used to “treat” diabetic conditions in some parts of Nigeria therefore the study, to investigate the extract of the leaves for activities on hyperglycaemia, lipid peroxidation, and increased cholesterol levelsin vivoin alloxan diabetic rats as well as its potential antioxidant activityin vitro.Methods. Albino rats (240–280 g) were given an injection of 120 mg/kg body weight, i.p. of alloxan monohydrate. After 8 days, diabetic animals with elevated fasting blood glucose levels (>9 mmol/L) were considered and selected for the study.Results. Oral treatment with the extract administered every 12 h by gavage at doses of 100, 200, and 400 mg/kg of the extract to the test rats, for 14 days, resulted in a significant dose-dependent decrease in blood glucose levels from 12.82 ± 1.02 mmol/dL to 4.92 ± 2.01 mmol/dL at the highest dose of 400 mg/kg compared to the control drug and glibenclamide as well as attendant significant decline in diabetic rats employed in the study.Conclusion. The extract also showedin vitroconcentration-dependent antioxidant activity following the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and ferric reducing assays. Findings further suggest the presence of active antidiabetic and antioxidant principles inM. oppositifoliusleaves.

scholarly journals Influence of Ocimum sanctum (L.) Extract on the Activity of Gliclazide in Alloxan-induced Diabetes in Rats

2020 ◽  
Vol 71 (11) ◽  
pp. 101-110
Author(s):  
Tayyeba Ramzan ◽  
Bilal Aslam ◽  
Faqir Muhammad ◽  
Muhammad Naeem Faisal ◽  
Asif Hussain
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Liver Tissue ◽  
Fasting Blood Glucose ◽  
Serum Levels ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
Protective Effects ◽  
Oxidative Stress Biomarkers ◽  
Glucose Levels

Ocimum sanctum L. is widely used as traditional remedy to manage hyperglycemia. This study was aimed to evaluate the effect of aqueous-methanolic extract of O. sanctum leaves (OSE) on the anti-diabetic activity of gliclazide in alloxan-induced diabetes in Wistar rats. Diabetes was induced by intraperitoneally injecting alloxan (120 mg/kg b.w.) in rats. Treatments including OSE (100 mg/kg b.w.), gliclazide (100 mg/kg b.w.), and in combination were given daily to diabetic rats till the 21st day of study. Body weight and fasting blood glucose levels were determined at regular intervals, while blood and organ samples were taken at the end of the study for biochemical and histopathological studies. Results showed that treatments exhibited anti-hyperglycemic activity through significantly (p [ 0.05) restoring body weight, fasting blood glucose level, and serum levels of glucose, insulin and HbA1c. The anti-lipidemic activity was noticed as total cholesterol (TC), triglyceride (TG), high-/low density-lipoproteins (HDL-C, LDL-C) levels were restored in treated diabetic rats. Ameliorative effects of treatments were observed as significant (p [ 0.05) reduction in serum levels of liver function biomarkers (alanine aminotransferase; ALT, aspartate aminotransferase; AST, alkaline phosphatase; ALP and bilirubin; BIL) and restoration of oxidative stress biomarkers (catalase; CAT, superoxide dismutase; SOD and malondialdehyde; MDA) in liver tissue. Histopathological findings supported these results as an increase in pancreatic islets size and protective effects on liver tissue was observed in diabetic rats treated with gliclazide and OSE alone and their combination. Conclusively, the combination of OSE and gliclazide produced a synergistic anti-diabetic effect as compared to that of alone treatment.

scholarly journals Effect Of Withania Coagulans And Liraglutide On Serum Glp-1, Postprandial And Fasting Blood Glucose In Streptozotocin Induced Diabetic Rats

2019 ◽  
Vol 09 (02) ◽  
pp. 120-123
Author(s):  
Abdul Samad ◽  
Noor Nasir Rajpoot ◽  
Hira Ayaz ◽  
Noman Sadiq
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Postprandial Blood Glucose ◽  
Controlled Study ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Withania Coagulans ◽  
Group B ◽  
Experimental Group

Objective: To evaluate effect of Withania coagulans and liraglutide on serum Glucagon like peptide-1, Postprandial and Fasting Blood Glucose levels in streptozotocin induced diabetic rat. Study Design and Setting: This randomize control trile was conducted at Islamic International Medical College in collaboration with National Institute of Health Islamabad. Methodology: This randomized controlled study was performed on a total of forty male Sprague dawly rats, which were initially divided into two groups; Group A (n=10) and Experimental Group (n=30). Diabetes in the Experimental group B was induced by intraperitoneal administration of streptozotocin for 5 days (30mg/kg/day). Diabetes was checked in experimental group by measuring fasting blood glucose (mg/dl) on day 6. Experimental group was further divided into Group B (Diabetic control), Group C (Withania coagulans-treated) and Group D (Liraglutide-treated). Blood sampling was done at day 30 and serum GLP-1, postprandial and fasting blood glucose levels were measured and compared in all groups. Results: Fasting and postprandial blood glucose levels of group C and D were significantly reduced as compared to group B. Serum GLP-1 levels were significantly increased in group C and D as compared to group B. Conclusion: Withania coagulans reduces hyperglycemia in diabetic rats through increasing GLP-1 hormone.

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