The mobility and reactivity of maleimide-binding proteins in the rat erythrocyte membrane. Effects of dietary zinc deficiency and incubation with zinc in vitro

1988 ◽  
Vol 66 (1) ◽  
pp. 66-71 ◽  
Author(s):  
Stan J. Kubow ◽  
William J. Bettger
Keyword(s):  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Zinc Deficiency ◽  
Binding Proteins ◽  
Thermal Sensitivity ◽  
Erythrocyte Membranes ◽  
Resonance Spectroscopy ◽  
Dietary Zinc ◽  
Membrane Effects

Erythrocyte ghosts, prepared from rats fed zinc-deficient diets, were analyzed for the mobility of membrane proteins by electron spin resonance spectroscopy of the sulfhydryl-binding spin probe, 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl. Compared with erythrocyte membranes from rats fed zinc-adequate diets ad libitum or pair-fed, erythrocyte membranes from zinc-deficient rats had a significantly increased ratio of weakly immobilized to strongly immobilized probe-binding proteins. This suggests that dietary zinc deficiency causes a conformational change in erythrocyte membrane proteins. Dietary zinc deficiency did not significantly affect N-ethylmaleimide (NEM)-induced thermal sensitivity or NEM-induced mechanical fragility in rat erythrocytes; however, the addition of zinc in vitro to red cells significantly inhibits NEM-induced mechanical fragility.

scholarly journals The Effect of Highly Hydroxylated Fullerenol C60(OH)36on Human Erythrocyte Membrane Organization

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jacek Grebowski ◽  
Anita Krokosz
Keyword(s):  
Membrane Proteins ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Conformational Changes ◽  
Protein Interactions ◽  
Erythrocyte Membranes ◽  
Resonance Spectroscopy ◽  
Protein Protein Interactions ◽  
Hydrophobic Region ◽  
Sh Groups

The mechanism of the interaction of highly hydroxylated fullerenol C60(OH)36with erythrocyte membranes was studied by electron spin resonance spectroscopy (ESR) of stearic acid derivatives labeled with a nitroxyl radical at C-12 or C-16 and with a nitroxyl derivative of maleimide covalently attached to sulfhydryl groups of membrane proteins. A significant increase in membrane fluidity in the hydrophobic region of the lipid bilayer was observed for 12-doxylstearic acid at fullerenol concentrations of 100 mg/L or 150 mg/L, while for 16-doxylstearic acid significant increase in fluidity was only observed at 150 mg/L. Fullerenol at 100 mg/L or 150 mg/L caused conformational changes in membrane proteins, expressed as an increase in thehw/hsparameter, when fullerenol was added before the maleimide spin label (MSL) to the membrane suspension. The increase of thehw/hsparameter may be caused by changes in lipid-protein or protein-protein interactions which increase the mobility of the MSL label and as a result increase the membrane fluidity. Incubation of the membranes with the MSL before the addition of fullerenol blocked the available membrane protein –SH groups and minimized the interaction of fullerenol with them. This confirms that fullerenol interacts with erythrocyte membrane proteins via available protein –SH groups.

scholarly journals Novel pathogenic XK mutations in McLeod syndrome and interaction between XK protein and chorein

2019 ◽  
Vol 5 (3) ◽  
pp. e328 ◽  
Author(s):  
Yuka Urata ◽  
Masayuki Nakamura ◽  
Natsuki Sasaki ◽  
Nari Shiokawa ◽  
Yoshiaki Nishida ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Molecular Analysis ◽  
Cultured Cells ◽  
Immunoblot Analysis ◽  
Erythrocyte Membranes ◽  
Onset Age ◽  
Novel Mutations ◽  
Erythrocyte Membrane Proteins ◽  
Normal Controls

ObjectiveTo identify XK pathologic mutations in 6 patients with suspected McLeod syndrome (MLS) and a possible interaction between the chorea-acanthocytosis (ChAc)- and MLS-responsible proteins: chorein and XK protein.MethodsErythrocyte membrane proteins from patients with suspected MLS and patients with ChAc, ChAc mutant carriers, and normal controls were analyzed by XK and chorein immunoblotting. We performed mutation analysis and XK immunoblotting to molecularly diagnose the patients with suspected MLS. Lysates of cultured cells were co-immunoprecipitated with anti-XK and anti-chorein antibodies.ResultsAll suspected MLS cases were molecularly diagnosed with MLS, and novel mutations were identified. The average onset age was 46.8 ± 8 years, which was older than that of the patients with ChAc. The immunoblot analysis revealed remarkably reduced chorein immunoreactivity in all patients with MLS. The immunoprecipitation analysis indicated a direct or indirect chorein-XK interaction.ConclusionsIn this study, XK pathogenic mutations were identified in all 6 MLS cases, including novel mutations. Chorein immunoreactions were significantly reduced in MLS erythrocyte membranes. In addition, we demonstrated a possible interaction between the chorein and XK protein via molecular analysis. The reduction in chorein expression is similar to that between Kell antigens and XK protein, although the chorein-XK interaction is a possibly noncovalent binding unlike the covalent Kell-XK complex. Our results suggest that reduced chorein levels following lack of XK protein are possibly associated with molecular pathogenesis in MLS.

Effect of Dietary Zinc Deficiency on the Endogenous Phosphorylation and Dephosphorylation of Rat Erythrocyte Membrane

1987 ◽  
Vol 117 (12) ◽  
pp. 2096-2105 ◽  
Author(s):  
Phyllis G. Paterson ◽  
O. Brian Allen ◽  
William J. Bettger
Keyword(s):  
Erythrocyte Membrane ◽  
Zinc Deficiency ◽  
Dietary Zinc

scholarly journals Effects of n-Octyl-β-D-Glucopyranoside on Human and Rat Erythrocyte Membrane Stability Against Hemolysis

2012 ◽  
Vol 5 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Cesare Sblano ◽  
Silvia Micelli ◽  
Daniela Meleleo
Keyword(s):  
Erythrocyte Membrane ◽  
Practical Importance ◽  
Human Erythrocytes ◽  
Erythrocyte Membranes ◽  
Ionic Surfactant ◽  
Hypotonic Medium ◽  
Rat Erythrocytes ◽  
Biphasic Effect ◽  
Dose Dependent

The practical importance for the pharmaceutical and cosmetics industries of the interactions between biological membranes and surfactant molecules has led to intensive research within this area. The interactions of non-ionic surfactant n-octyl-β-D-glucopyranoside (OG) with the human and rat erythrocyte membranes were studied. The in vitro hemolytic and antihemolytic activities were determined by employing a method in which both erythrocytes were added to the hypotonic medium containing OG at different concentrations, and the amount of haemoglobin released was determined. noctyl- β-D-glucopyranoside was found to have a biphasic effect on both types of erythrocyte membrane. We also investigated the interactions of OG with the erythrocyte membrane in isotonic medium; the dose-dependent curves show similar behaviour in both human and rat erythrocytes. Our results showed that OG has greater antihemolytic potency on rat than on human erythrocytes; furthermore, rat erythrocytes were more sensitive than human erythrocytes to hypotonic shock. How the different lipoprotein structure of these erythrocytes determines a difference in antihemolytic activity is discussed.

MORPHOFUNCTIONAL PARAMETERS OF CATTLE ERYTHROCYTES UNDER STRESS AND ITS CORRECTION BY LOW-INTENSITY LASER RADIATION

1970 ◽  
pp. 67-71
Author(s):  
М. N. Ivashchenko ◽  
А. V. Deryugina ◽  
P. S. Ignatiev ◽  
V. B. Metelin ◽  
М. N. Talamanova ◽  
...  
Keyword(s):  
Laser Radiation ◽  
Erythrocyte Membrane ◽  
Control Level ◽  
Experimental Studies ◽  
Erythrocyte Membranes ◽  
Physical Factors ◽  
Low Intensity ◽  
Intensity Laser ◽  
Technological Stress

The study investigated the content of malondialdehyde (MDA), the electrophoretic mobility of erythrocytes (EPME), the spectrum of erythrocyte membrane proteins and the morphology of cattle erythrocytes in in vitro experiments under stress and exposure to low-intensity laser radiation (LLLT). Clinical and experimental studies carried out in the last decade indicate the possibility of modulating the organism adaptive reactions when exposed to such physical factors as low-intensity laser radiation. The work showed that the effect of LLLT on the blood of stressed animals caused the restoration of the studied parameters to the level of physiological norms, while in animals that underwent technological stress, EPME was reduced by 31%, MDA concentration was increased by 65%. The effect of LLLT on the blood of unstressed animals did not lead to a change in EPME and MDA concentration. The study of the protein spectrum of erythrocyte membrane of animals subjected to technological stress revealed that the content of spectrin decreased by 16%, glycophorin C increased by 35%, the morphology of erythrocytes after stress was characterized by an increase in the number of echinocytes, stomatocytes and degeneratively altered erythrocytes. Under the LLLT action on the cow erythrocytes after stress there was a restoration of the morphology of cells and the content of proteins of erythrocyte membranes to the control level.

scholarly journals Lipid composition and fluidity of the erythrocyte membrane in copper-deficient rats

1992 ◽  
Vol 68 (2) ◽  
pp. 435-443 ◽  
Author(s):  
K. M. Abu-Salah ◽  
A. A. Alothman ◽  
K. Y. Lei
Keyword(s):  
Fatty Acid ◽  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
Lipid Composition ◽  
Phospholipid Fatty Acid ◽  
Sodium Dodecyl ◽  
Erythrocyte Membranes ◽  
Molar Ratio ◽  
Excimer Fluorescence ◽  
Cu Deficiency

The influence of dietary copper on lipid composition, phospholipid-fatty acid and protein profiles and fluidity of the erythrocyte membranes of rats is reported. In general Cu deficiency in rats induced some changes in the phospholipid-fatty acid profile of erythrocyte membranes when compared with Cu-adequate animals. Stearic (18:0) and docosadienoic (22:2n-3) acids contents, for example, were significantly increased (P< 0.001) while oleic (18:1n-9) and linolenic (18:3n-3) acid contents were significantly depressed (P< 0.001) as a result of Cu deficiency. Moreover the cholesterol:phospholipids molar ratio and the cholesterol (mol):membrane proteins (mg) ratio in Cu-deficient rats were, to different degrees, significantly lower than in animals fed on Cu-adequate diets. In addition, diets deficient in Cu led to a reduction in erythrocyte membrane fluidity (P< 0.001) as assessed by the intramolecular excimer fluorescence of 1,3-di(1-pyrenyI) propane. However, no significant alteration in the phospholipid:protein ratio was observed as a result of differences in dietary treatment. The pattern of erythrocyte membrane proteins obtained with sodium dodecyl sulphate-polyacrylamide gel electro-phoresis did not seem to be influenced by Cu-deficient diets.

scholarly journals Changes in erythrocyte membrane proteins during in vitro aging

1987 ◽  
Vol 43 ◽  
pp. 95
Author(s):  
Kenji Yamamoto ◽  
Miho Yamada ◽  
Eiko Ueno ◽  
Haruki Uemura ◽  
Yuzo Kato
Keyword(s):  
Membrane Proteins ◽  
Erythrocyte Membrane ◽  
In Vitro Aging ◽  
Erythrocyte Membrane Proteins

scholarly journals An antigen of Plasmodium yoelii that translocates into the mouse erythrocyte membrane upon entry into the host cell

1985 ◽  
Vol 73 (1) ◽  
pp. 311-320
Author(s):  
K. Murakami ◽  
K. Tanabe
Keyword(s):  
Host Cell ◽  
Erythrocyte Membrane ◽  
Plasmodium Yoelii ◽  
Erythrocyte Membranes ◽  
Rodent Malaria Parasite ◽  
Strong Fluorescence ◽  
Cytoplasmic Surface ◽  
Parasite Plasmodium ◽  
Mouse Erythrocyte

Monoclonal antibodies against the rodent malaria parasite, Plasmodium yoelii, have been prepared and characterized by indirect immunofluorescence on acetone-fixed infected mouse erythrocytes. The antibody of clone K2 reacted strongly with late trophozoites and schizonts, whereas it did so weakly and diffusely with ring forms and early trophozoites. Strong fluorescence was confined to granular structures in schizonts and merozoites. Parasites that invaded erythrocytes in vitro lost the strong fluorescence. Instead, immunofluorescence appeared in the membranes of erythrocytes infected in vitro with merozoites. Erythrocytes infected with more than one merozoite had intensified immunofluorescence in their membranes. Staining of the invaded erythrocytes with 4′,6-diamidino-2-phenylindole (DAPI) hydrochloride demonstrated that membranes of all the invaded erythrocytes acquired the P. yoelii antigen. These results suggest that the P. yoelii antigen in merozoites is translocated into erythrocyte membranes upon entry into the host cell. Immunofluorescence continued to appear in membranes of infected erythrocytes throughout the intra-erythrocytic parasite growth. Staining of unfixed infected erythrocytes with the K2 antibody failed to detect the parasite antigen. In contrast, immunofluorescence was present in unfixed membranes of erythrocyte ghosts, which had been spontaneously formed after rupture of schizont-infected erythrocytes by merozoite release. No immunofluorescence appeared in either acetone-fixed or unfixed ghosts of normal erythrocytes. These results suggest the antigenic determinant of the P. yoelii antigen is exposed at the cytoplasmic surface of the infected erythrocyte membrane. Immunoprecipitation has revealed that the K2 antibody recognizes a 160 X 10(3) Mr P. yoelii antigen.

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