Adaptive changes in rat liver plasma membranes after chronic alcohol administration and its subsequent withdrawal

1986 ◽  
Vol 64 (1) ◽  
pp. 85-92 ◽  
Author(s):  
Hung Lee ◽  
E. A. Hosein
Keyword(s):  
Plasma Membranes ◽  
Membrane Lipids ◽  
Buoyant Density ◽  
Fatty Acyl ◽  
Chronic Alcohol ◽  
Alcohol Administration ◽  
Rat Liver Plasma Membranes ◽  
Subsequent Withdrawal ◽  
Induced Changes ◽  
Chronic Alcohol Administration

Rat hepatic plasma membranes isolated after chronic alcohol feeding displayed a different buoyant density range with a significantly increased peak density value when spun isopycnically in a 30–50% sucrose (w/w) gradient. This change persisted up to 48 h of withdrawal from alcohol. Analysis of membrane lipids revealed certain significant alterations in the phospholipids as well as the fatty acyl composition in individual phospholipids of the experimental plasma membranes. During withdrawal of alcohol for 48 h, all the alcohol-induced changes in the phospholipids returned to normal. Most initial changes in fatty acids reverted to the control composition during this time, but new changes in fatty acyl distribution were also observed. These were interpreted to represent readaptation to the withdrawal of the alcohol. It is not established how long this readaptation period lasts.

Chronic alcohol feeding and its withdrawal on the structure and function of the rat liver plasma membrane: a study with 125I-labelled glucagon binding as a metabolic probe

1982 ◽  
Vol 60 (9) ◽  
pp. 1171-1176 ◽  
Author(s):  
Hung Lee ◽  
E. A. Hosein
Keyword(s):  
Plasma Membrane ◽  
Rat Liver ◽  
Plasma Membranes ◽  
Structure And Function ◽  
Scatchard Analysis ◽  
Chronic Alcohol ◽  
Alcohol Administration ◽  
Liver Plasma Membranes ◽  
And Function ◽  
Chronic Alcohol Administration

The effect of chronic alcohol administration on the structure and function of the rat liver plasma membranes has been investigated. Chronic alcohol administration did not affect the yield of these membranes using conventional isolation procedures. The extent of plasma membrane enrichment or contamination with other interior membranes was identical in the control and alcoholic preparations. The binding of 125I-labelled glucagon to these experimental liver plasma membranes was significantly decreased. Scatchard analysis of the high affinity sites showed a significant reduction [Formula: see text] in receptor number rather than binding affinity, which was not altered. This anomaly persisted through 72-h withdrawal of alcohol. These data suggest that very stable changes were induced in these liver plasma membranes after prolonged alcohol ingestion.

THE EFFECT OF CHRONIC ALCOHOL ADMINISTRATION UPON CARDIAC NUCLEOTIDE CONCENTRATIONS AND HISTOMORPHOMETRY

1994 ◽  
Vol 22 (3) ◽  
pp. 352S-352S
Author(s):  
VINOOD B. PATEL ◽  
JONATHAN R. SALISBURY ◽  
LORETA M. RODRIGUES ◽  
JOHN R. GRIFFITHS ◽  
PETER J. RICHARDSON ◽  
...  
Keyword(s):  
Chronic Alcohol ◽  
Alcohol Administration ◽  
Chronic Alcohol Administration

scholarly journals Differential expression of microRNAs in the hippocampi of male and female rodents after chronic alcohol administration

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Mi Ran Choi ◽  
Jasmin Sanghyun Han ◽  
Yeung-Bae Jin ◽  
Sang-Rae Lee ◽  
In Young Choi ◽  
...  
Keyword(s):  
Alcohol Use ◽  
Target Genes ◽  
The Other ◽  
Female Rats ◽  
Chronic Alcohol ◽  
Male And Female ◽  
Heavy Alcohol ◽  
Alcohol Administration ◽  
Heavy Alcohol Use ◽  
Chronic Alcohol Administration

Abstract Background Women are more vulnerable than men to the neurotoxicity and severe brain damage caused by chronic heavy alcohol use. In addition, brain damage due to chronic heavy alcohol use may be associated with sex-dependent epigenetic modifications. This study aimed to identify microRNAs (miRNAs) and their target genes that are differentially expressed in the hippocampi of male and female animal models in response to alcohol. Methods After chronic alcohol administration (3~3.5 g/kg/day) in male (control, n = 10; alcohol, n = 12) or female (control, n = 10; alcohol, n = 12) Sprague-Dawley rats for 6 weeks, we measured body weights and doublecortin (DCX; a neurogenesis marker) concentrations and analyzed up- or downregulated miRNAs using GeneChip miRNA 4.0 arrays. The differentially expressed miRNAs and their putative target genes were validated by RT-qPCR. Results Alcohol attenuated body weight gain only in the male group. On the other hand, alcohol led to increased serum AST in female rats and decreased serum total cholesterol concentrations in male rats. The expression of DCX was significantly reduced in the hippocampi of male alcohol-treated rats. Nine miRNAs were significantly up- or downregulated in male alcohol-treated rats, including upregulation of miR-125a-3p, let-7a-5p, and miR-3541, and downregulation of their target genes (Prdm5, Suv39h1, Ptprz1, Mapk9, Ing4, Wt1, Nkx3-1, Dab2ip, Rnf152, Ripk1, Lin28a, Apbb3, Nras, and Acvr1c). On the other hand, 7 miRNAs were significantly up- or downregulated in alcohol-treated female rats, including downregulation of miR-881-3p and miR-504 and upregulation of their target genes (Naa50, Clock, Cbfb, Arih1, Ube2g1, and Gng7). Conclusions These results suggest that chronic heavy alcohol use produces sex-dependent effects on neurogenesis and miRNA expression in the hippocampus and that sex differences should be considered when developing miRNA biomarkers to diagnose or treat alcoholics.

Myoblast Mitochondrial Adaptation to Chronic Alcohol Administration

2020 ◽  
Vol 34 (S1) ◽  
pp. 1-1
Author(s):  
Danielle E. Levitt ◽  
Liz Simon ◽  
Patricia E. Molina
Keyword(s):  
Chronic Alcohol ◽  
Alcohol Administration ◽  
Chronic Alcohol Administration ◽  
Mitochondrial Adaptation

Water Metabolism in Rats Subjected to Chronic Alcohol Administration

2004 ◽  
Vol 97 (1) ◽  
pp. p9-p15 ◽  
Author(s):  
Alexandr Parlesak ◽  
Claus Pohl ◽  
J. Christian Bode ◽  
Christiane Bode
Keyword(s):  
Chronic Alcohol ◽  
Water Metabolism ◽  
Alcohol Administration ◽  
Chronic Alcohol Administration

Effects of chronic alcohol administration on synaptic membrane Na+Ca2+ exchange activity

1987 ◽  
Vol 414 (2) ◽  
pp. 239-244 ◽  
Author(s):  
M.L. Michaelis ◽  
E.K. Michaelis ◽  
E.W. Nunley ◽  
N. Galton
Keyword(s):  
Synaptic Membrane ◽  
Chronic Alcohol ◽  
Alcohol Administration ◽  
Chronic Alcohol Administration ◽  
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