Autoradiographic localization of binding sites for atrial natriuretic factor

1985 ◽  
Vol 63 (11) ◽  
pp. 1373-1377 ◽  
Author(s):  
H. P. von Schroeder ◽  
E. Nishimura ◽  
C. H. S. McIntosh ◽  
A. M. J. Buchan ◽  
N. Wilson ◽  
...  
Keyword(s):  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Renal Medulla ◽  
Rabbit Aorta ◽  
Wistar Rat ◽  
Iliac Vein ◽  
Zona Glomerulosa ◽  
Rat Tissues ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

The distribution of atrial natriuretic factor (ANF) binding sites in Wistar rat tissues, as well as tissues from other species was studied. Using autoradiography of slide mounted tissue sections incubated with 125I-labelled ANF, high densities of binding sites were found in the renal glomeruli and papilla, aortic smooth muscle, iliac vein, choroid plexus, anterior pituitary, lung, and adrenal zona glomerulosa. Results from renal, aortic, adrenal, and lung tissues from spontaneously hypertensive rats did not differ from those of Wistar rats. Binding sites were also observed in guinea pig glomeruli, renal medulla, and aorta as well as in the rabbit aorta.

Atrial natriuretic factor binding sites in the jejunum

1989 ◽  
Vol 256 (2) ◽  
pp. G436-G441 ◽  
Author(s):  
C. Bianchi ◽  
G. Thibault ◽  
A. De Lean ◽  
J. Genest ◽  
M. Cantin
Keyword(s):  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Specific Binding ◽  
Rat Tissues ◽  
Rat Jejunum ◽  
Specific Binding Sites ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

We have studied the localization and the characterization of atrial natriuretic factor (ANF) binding sites by radioautographic techniques. Quantitative in vitro radioautography with a computerized microdensitometer demonstrated the presence of high-affinity, low-capacity 125I-ANF-(99-126) binding sites (Kd, 48 pM; Bmax, 63 fmol/mg protein) mainly in the villi of 20-microns slide-mounted transverse sections of the rat jejunum. Competition curves showed 50% inhibitory concentrations of 55 and 1,560 pM for ANF-(99-126) and ANF-(103-123), respectively. In vivo electron microscope radioautography showed that 80% of the silver grains were localized on the lamina propria fibroblast-like cells, 18% on mature enterocytes, and 2% on capillaries. Bradykinin and adrenocorticotropin did not compete with ANF binding. These results demonstrate that ANF binding sites in the rat jejunum possess the pharmacological characteristics of functional ANF receptors encountered in other rat tissues, and ultrastructural radioautographs show their cellular distribution. Taken together, these results demonstrate the presence and the localization of specific binding sites for ANF in the jejunal villi of the rat small intestine.

scholarly journals Localization of 125I-atrial natriuretic factor (ANF)-binding sites in rat renal medulla. A light and electron microscope autoradiographic study.

1987 ◽  
Vol 35 (2) ◽  
pp. 149-153 ◽  
Author(s):  
C Bianchi ◽  
J Gutkowska ◽  
R Garcia ◽  
G Thibault ◽  
J Genest ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Renal Medulla ◽  
Microscopic Level ◽  
Electron Microscopic Level ◽  
Natriuretic Factor ◽  
Vasa Recta ◽  
Collecting Ducts ◽  
Atrial Natriuretic

Using light and electron microscope autoradiography in vivo, the localization of 125I-(Arg 101-Tyr 126) atrial natriuretic factor (ANF)-binding sites was studied in the renal medulla of rats. At the light microscopic level, the autoradiographic reaction was mainly distributed in patches in the outer medulla, and followed the tubular architecture in the innermost part of the inner medulla. At the electron microscopic level, binding sites were mainly found in the outer medullary descending vasa recta and inner medullary collecting ducts. These results suggest that, in rats, the renal medulla may participate in the natriuresis and diuresis produced by ANF through vascular and tubular effects; the former by changing medullary blood flow at the level of descending vasa recta and the latter by acting on electrolyte and water transport at the level of collecting ducts.

Atrial natriuretic factor binding sites in experimental congestive heart failure

1989 ◽  
Vol 257 (4) ◽  
pp. F515-F523
Author(s):  
C. Bianchi ◽  
G. Thibault ◽  
E. Wrobel-Konrad ◽  
A. De Lean ◽  
J. Genest ◽  
...  
Keyword(s):  
Heart Failure ◽  
Congestive Heart Failure ◽  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Severe Heart Failure ◽  
Zona Glomerulosa ◽  
Renal Glomeruli ◽  
Natriuretic Factor ◽  
Cardiomyopathic Hamsters ◽  
Atrial Natriuretic

A quantitative in vitro autoradiographic study was performed on the aorta, renal glomeruli, and adrenal cortex of cardiomyopathic hamsters in various stages of heart failure and correlated, in some instances, with in vivo autoradiography. The results indicate virtually no correlation between the degree of congestive heart failure and the density of 125I-labeled atrial natriuretic factor [(Ser99, Tyr126)ANF] binding sites (Bmax) in the tissues examined. Whereas the Bmax was increased in the thoracic aorta in moderate and severe heart failure, there were no significant changes in the zona glomerulosa. The renal glomeruli Bmax was lower in mild and moderate heart failure compared with control and severe heart failure. The proportion of ANF B- and C-receptors was also evaluated in sections of the aorta, adrenal, and kidney of control and cardiomyopathic hamsters with severe heart failure. (Arg102, Cys121)ANF [des-(Gln113, Ser114, Gly115, Leu116, Gly117) NH2] (C-ANF) at 10(-6) M displaced approximately 505 of (Ser99, Tyr126)125I-ANF bound in the aorta and renal glomeruli and approximately 20% in the adrenal zona glomerulosa in both series of animals. These results suggest that ANF may exert a buffering effect on the vasoconstriction of heart failure and to a certain extent may inhibit aldosterone secretion. The impairment of renal sodium excretion does not appear to be related to glomerular ANF binding sites at any stage of the disease.

scholarly journals Atrial natriuretic factor mRNA and binding sites in the adrenal gland

1990 ◽  
Vol 271 (2) ◽  
pp. 555-558 ◽  
Author(s):  
D J R Nunez ◽  
A P Davenport ◽  
M J Brown
Keyword(s):  
Angiotensin Ii ◽  
Adrenal Gland ◽  
Adrenal Medulla ◽  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Zona Glomerulosa ◽  
Aldosterone Secretion ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

The factor inhibiting aldosterone secretion produced by the adrenal medulla may be atrial natriuretic factor (ANF), since the latter abolishes aldosterone release in response to a number of secretagogues, including angiotensin II and K+. In this study we have shown that cells in the adrenal medulla contain ANF mRNA and therefore have the potential to synthesize this peptide. The presence of binding sites for ANF predominantly in the adrenal zona glomerulosa suggests that, if ANF is synthesized in the medulla and transferred to the cortex, it may affect mineralocorticoid status.

Distinct localization of atrial natriuretic factor and angiotensin II binding sites in the glomerulus

1986 ◽  
Vol 251 (4) ◽  
pp. F594-F602 ◽  
Author(s):  
C. Bianchi ◽  
J. Gutkowska ◽  
G. Thibault ◽  
R. Garcia ◽  
J. Genest ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Comparative Study ◽  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Mesangial Cells ◽  
Ang Ii ◽  
Half Maximum ◽  
Natriuretic Factor ◽  
Glomerular Size ◽  
Atrial Natriuretic

A comparative study of the localization of 125I-labeled atrial natriuretic factor (ANF) and 125I-labeled angiotensin II (ANG II) binding sites in the glomerulus of the rat, after an intravascular injection, has been done by ultrastructural radioautography. 125I-ANF binding sites are localized predominantly on the podocytes of the visceral epithelium (63%) followed by the endothelium of capillaries (14%), the parietal epithelium (13%), and finally mesangial cells (10%). In a comparative study, it was confirmed that 125I-ANG II uptake is localized predominantly on mesangial cells (60%) followed by epithelial visceral cells (23%) and the endothelium of capillaries (16%). Using isolated rat glomeruli, it was confirmed that ANG II decreases glomerular size (maximum effect of 15%) with an apparent half maximum effective concentration (EC50) between 10(-9) and 10(-8) M. Although ANF alone has no apparent effect on glomerular size, it inhibits the contractile effect of ANG II with a half maximum inhibitory concentration (IC50) between 10(-11) and 10(-10) M. These results suggest that an intraglomerular mechanism other than glomerular arteriolar resistance may be involved in the modulation of glomerular filtration rate by ANF. The presence of 125I-ANF uptake mainly in foot processes of visceral epithelial cells of glomeruli in vivo and the inhibition of ANG II decrease in glomerular size by ANF in vitro raise the possibility that ANF may regulate the ultrafiltration coefficient by two mechanisms: modulation of glomerular permeability, and surface area.

Atrial natriuretic factor (ANF) binding sites in brain and related structures

Peptides ◽  
1984 ◽  
Vol 5 (6) ◽  
pp. 1167-1172 ◽  
Author(s):  
Rémi Quirion ◽  
Michel Dalpé ◽  
André De Lean ◽  
J. Gutkowska ◽  
Marc Cantin ◽  
...  
Keyword(s):  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

Radioautographic Localization of 125I-Atrial Natriuretic Factor Binding Sites in the Brain

1986 ◽  
Vol 44 (3) ◽  
pp. 365-372 ◽  
Author(s):  
Césario Bianchi ◽  
J. Gutkowska ◽  
M. Ballak ◽  
G. Thibault ◽  
R. Garcia ◽  
...  
Keyword(s):  
Binding Sites ◽  
Atrial Natriuretic Factor ◽  
Factor Binding ◽  
Natriuretic Factor ◽  
The Brain ◽  
Atrial Natriuretic
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