Use of 3-O-methyI-D-glucose and phloretin to estimate the intracellular water space of mouse peritoneal macrophage monolayers

1982 ◽  
Vol 60 (1) ◽  
pp. 76-79
Author(s):  
Douglas B. Lowrie
Keyword(s):  
Glucose Uptake ◽  
Peritoneal Macrophage ◽  
Intracellular Water ◽  
Mouse Peritoneal Macrophage ◽  
Facilitated Diffusion ◽  
Water Space ◽  
Glucose Carrier

Macrophages took up 3-O-methyl-D-glucose rapidly by facilitated diffusion using the glucose carrier and slowly by carrier-independent diffusion. Phloretin inhibited carrier-dependent but not carrier-independent diffusion. Estimates of intracellular water space based on 3-O-methyl-D-glucose uptake varied between 0.7 and 6.9 μL∙106 cells−1 for 2-week-old monolayers.

scholarly journals Effect of photobiomodulation therapy on the regulation of glucose uptake by lymphocytes in diabetes mellitus (Review)

2021 ◽  
Vol 15 (4) ◽  
pp. 87-104
Author(s):  
A. O. Maslakova ◽  
◽  
M. Ya. Liuta ◽  
N. O. Sybirna ◽  
◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Glucose Uptake ◽  
Glucose Utilization ◽  
Inflammatory Responses ◽  
Glucose Transporters ◽  
Primary Energy ◽  
Facilitated Diffusion ◽  
Immune Functions ◽  
Elevated Glucose ◽  
Glucose Carrier

For most cells, including lymphocytes, glucose is a primary energy source, and, therefore, it is vital to understand the regulatory mechanisms that control the work of glucose transporters. Lymphocytes are pivotal for mediation of immune and inflammatory responses. A feature of lymphocytes is increasing glucose utilization during activation of the immune function, which is strongly dependent on glucose uptake. Some studies show that elevated glucose concentration in diabetes mellitus affects lymphocytes’ glucose transporters expression, whichcorrelates with impaired immune functions and may become one of the predisposing factors of contracting infectious diseases. Recent studies have focused on glucose transporters as therapeutic targets for a variety of diseases, including diabetes mellitus. This review demonstrates the effect of photobiomodulationtherapy on glucose uptake by Na+-coupled glucose carrier SGLT1 and facilitated diffusion glucose carriers of the GLUT family (GLUT1, GLUT3, GLUT4) in normal and diabetic lymphocytes.

Macrophage Inhibition of Collagen-Induced Platelet Aggregation

1979 ◽  
Vol 42 (04) ◽  
pp. 1207-1216 ◽  
Author(s):  
Berit Mørland
Keyword(s):  
Enzyme Activity ◽  
Platelet Aggregation ◽  
Peritoneal Macrophage ◽  
Cultured Cells ◽  
Culture Media ◽  
Human Platelets ◽  
Mouse Peritoneal Macrophage ◽  
Collagenase Activity ◽  
Partial Inhibition ◽  
Macrophage Phagocytosis

SummaryCollagen was incubated with cells or media fractions of mouse peritoneal macrophage cultures, and its aggregating effect on human platelets was tested. Incubation with lysates of cultured cells completely abolished the normal collagen-induced platelet aggregation, while incubation with media fractions only caused partial inhibition. The latter inhibition was more pronounced after macrophage phagocytosis of latex particles, while endocytosis of endotoxin had no effect.Corresponding macrophage cultures were also tested for specific collagenase activity, using 14C-glycine labelled collagen as substrate. Collagenase activity was found in the culture media fractions only, and the enzyme activity could be enhanced by endocytosis of latex as well as endotoxin.It appears that the effect of macrophage lysates and media on collagen-platelet interaction cannot be ascribed only to secretion of collagenase from macrophages.

Effect of Lectins on Mouse Peritoneal Macrophage Phagocytic Activity

1994 ◽  
Vol 23 (6-7) ◽  
pp. 429-436 ◽  
Author(s):  
Guadalupe Maldonado ◽  
Flor Porras ◽  
Leonor Fernández ◽  
Lorena Vázquez ◽  
Edgar Zenteno
Keyword(s):  
Peritoneal Macrophage ◽  
Phagocytic Activity ◽  
Mouse Peritoneal Macrophage

scholarly journals Pogonatherumol, a Novel Highly Oxygenated Norsesquiterpene with Flavone C-Glycosides from Pogonatherum crinitum

2018 ◽  
Vol 2018 ◽  
pp. 1-3
Author(s):  
Lin Ni ◽  
Wei Huang ◽  
He-shan Wang ◽  
Hui-you Xu
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Peritoneal Macrophage ◽  
Spectroscopic Data ◽  
Human Cancer ◽  
Mouse Peritoneal Macrophage ◽  
No Production ◽  
Human Cancer Cell ◽  
X Ray ◽  
Human Cancer Cell Lines

A novel highly oxygenated norsesquiterpene, pogonatherumol (1), with two known flavone C-glycosides (2-3), was isolated from Pogonatherum crinitum. The structure of the new compound was illuminated based on its spectroscopic data and X-ray analysis. Compounds 1 and 3 inhibited NO production in the mouse peritoneal macrophage (64.5 ± 7.2% and 61.6 ± 5.8%, respectively, at a concentration of 50 μM). The three compounds were inactive when tested against two human cancer cell lines (IC50 values > 50 μM).

scholarly journals Characterization of adenylyl cyclase stimulated by VIP in rat and mouse peritoneal macrophage membranes

1996 ◽  
Vol 1312 (3) ◽  
pp. 249-254 ◽  
Author(s):  
David Pozo ◽  
J.José Segura ◽  
Isabel Carrero ◽  
Luis G. Guijarro ◽  
Juan C. Prieto ◽  
...  
Keyword(s):  
Adenylyl Cyclase ◽  
Peritoneal Macrophage ◽  
Mouse Peritoneal Macrophage

scholarly journals Increased Multiplication of Dengue Virus in Mouse Peritoneal Macrophage Cultures by Treatment with Extracts of Ascaris-Parascaris Parasites

1985 ◽  
Vol 29 (4) ◽  
pp. 337-348 ◽  
Author(s):  
Agus S. Wiharta ◽  
Hak Hotta ◽  
Susumu Hotta ◽  
Takeo Matsumura ◽  
Sujudi ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Peritoneal Macrophage ◽  
Mouse Peritoneal Macrophage

scholarly journals Platelet Water Content is Decreased by Gel-Filtration

1977 ◽  
Author(s):  
J.S. Wiley ◽  
G. Wray ◽  
I.A. Cooper
Keyword(s):  
Water Content ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Intracellular Water ◽  
Total Water ◽  
Initial Water ◽  
Mean Cell Volume ◽  
Cell Pellet ◽  
The Mean ◽  
Water Space

One approach to platelet sizing is to measure the intracellular water space of platelets with 3H-H2O since the % water content of platelets remains constant in states with different platelet sizes. Fresh citrated blood was centrifuged for 10 min at 150 'g' to obtain PRP. Aliquots of PRP were briefly incubated with either 3H-H2O or 14C-sucrose then layered over 0.3 ml dibutylphthalate and spun 4 min at 8000 'g'. The cell pellet was solubilized and counted to enable spaces to be calculated. The extracellular (sucrose) space was subtracted from the total water space of the pellet to give a mean intracellular water space of 0.56 ± 0.12 μ1/108 platelets (n =19). Assuming a water content of 7 5% and a density of 1.04, the mean platelet volume for normal subjects is 7.2 fl. Gel-filtration of platelets (GFP) on Sepharose-2B reduced their mean water space by 0.12 μl/108 platelets. However the amount of shrinkage on gel-filtration depended on the initial water space of the platelets in PRP and there was a linear relation between these two variables (r = 0.82). Shrinkage was 40% for an initial platelet water space of 0.70 μl/108 platelets but there was almost no shrinkage below a water space of 0.40 μl/108 platelets. Recovery of platelets from each column averaged 8 0% and showed no relation to the reduction in the mean cell water space. The lower water space of GFP may indicate a reduction in mean cell volume due to gel-filtration.

Wheat-germ agglutinin binding in four types of mouse peritoneal macrophage

1984 ◽  
Vol 80 (5) ◽  
pp. 449-456 ◽  
Author(s):  
R. de Water ◽  
J. M. van't Noordende ◽  
W. Th. Daems ◽  
L. A. Ginsel
Keyword(s):  
Peritoneal Macrophage ◽  
Wheat Germ Agglutinin ◽  
Wheat Germ ◽  
Mouse Peritoneal Macrophage

scholarly journals Signal regulatory protein α1 is involved in the inhibitory effect of glucocorticoid receptor on the proliferation of murine macrophage RAW264.7 cell and mouse peritoneal macrophage

2008 ◽  
Vol 41 (5) ◽  
pp. 393-403 ◽  
Author(s):  
Xiaohui Wang ◽  
Yidong Li ◽  
Xiaoyan Zhu ◽  
Yan Wang ◽  
Fei Diao ◽  
...  
Keyword(s):  
Rna Interference ◽  
Peritoneal Macrophage ◽  
Inflammatory Responses ◽  
Regulatory Protein ◽  
Mouse Peritoneal Macrophage ◽  
Raw264.7 Cells ◽  
Dependent Manner ◽  
Proliferation Inhibition ◽  
Murine Macrophage ◽  
Concentration Dependent Manner

Glucocorticoid (GC) effectively suppresses immune and inflammatory responses and inhibits the growth of several types of cells, but the role of GC and its receptor on macrophage proliferation is unclear. In our previous work, we found RAW-GR(−) cells (murine macrophage RAW264.7 cells stably transfected with GR-siRNA expression vector by RNA interference) grew faster by about twofold. In this study, we further explored the role and mechanisms of GC/GR on the proliferation of macrophage. We found that the growth of RAW264.7 cells was inhibited by dexamethasone (Dex) in a concentration-dependent manner. The mRNA and protein levels of signal regulatory protein α1 (SIRPA) were induced by GC/GR in RAW264.7 cells and SIRPA expression was decreased remarkably in RAW-GR(−) cells. Overexpression of SIRPA negatively regulated the proliferation of RAW-GR(−) cells, and inhibition of SIRPA expression by a small from RNA interference attenuated Dex-induced proliferation inhibition in RAW264.7 cells. The proliferation inhibition of GC/GR was also found in mouse peritoneal macrophage, which was associated with the increase in SIRPA induced by GC/GR as well. In addition, elevation of the expression of CDK2, cyclinD1, and cyclinB1, but not phosphorylated ERK1/2 and p38, was found in RAW-GR(−) cells. In conclusion, we provided the novel evidences that GC/GR inhibited the growth of RAW264.7 cells and mouse peritoneal macrophage, and the antiproliferative effect of GC/GR on these cells was at least in part a result from GC/GR-induced SIRPA expression. Up-regulation of CDK2, cyclinD1, and cyclinB1 was also related to the increased proliferation of RAW-GR(−) cells.

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