Some observations on the behaviour of chloride current–voltage relations in Xenopus muscle membrane in acid solutions

1981 ◽  
Vol 59 (1) ◽  
pp. 7-13 ◽  
Author(s):  
Donald D. F. Loo ◽  
James G. McLarnon ◽  
Peter C. Vaughan
Keyword(s):  
Steady State ◽  
Standard Test ◽  
Chloride Current ◽  
Chloride Conductance ◽  
Resting Potential ◽  
Single Type ◽  
Muscle Membrane ◽  
Wave Form ◽  
Solution Ph ◽  
Current Voltage

Chloride current–voltage relations in Xenopus laevis muscle membrane have been investigated in phosphate-buffered solution (pH 5.2–5.4) using a three-microelectrode voltage clamp. Resting chloride conductance in these conditions is about 10−4 S/cm2, approximately [Formula: see text]th that at pH 8.8. When the membrane potential is stepped from the holding (resting) potential to a more negative voltage the current rises from the initial to the steady state. The instantaneous current–voltage relation is linear and the steady-state relation shows inward-going rectification. As hyperpolarization appears to "activate" the chloride conductance, the "availability" of chloride current has been measured at the beginning of a voltage step to a standard test potential following conditioning at a variety of potentials. The relationship between the test current and the conditioning voltage is sigmoid. The normalized sigmoid curve has the same slope (absolute value) but opposite sign to that obtained in the same experiment at pH 8.8. In mildly acidic solutions (pH 6.4) the current wave form is diphasic: current initially falls then rises to the steady state. This combination of transients militates against the idea that transients are due solely to accumulation–depletion effects in restricted spaces ("unstirred layers") and a hypothesis is qualitatively outlined in which pH- and voltage-dependent effects are ascribed to a single type of channel whose orientation in the membrane is unconstrained.

Voltage dependence of chloride current through Xenopus muscle membrane in alkaline solutions

1980 ◽  
Vol 58 (9) ◽  
pp. 999-1010 ◽  
Author(s):  
Peter C. Vaughan ◽  
James G. McLarnon ◽  
Donald D. F. Loo
Keyword(s):  
Steady State ◽  
Membrane Potential ◽  
Chloride Conductance ◽  
Resting Potential ◽  
Muscle Membrane ◽  
Alkaline Solutions ◽  
Constant Field ◽  
Confined Space ◽  
Initial Current ◽  
Test Current

Three-microelectrode voltage-clamp experiments have been conducted on surface fibres of Xenopus laevis sartorius muscles. When potassium and chloride were substituted by rubidium and sulphate, negligibly small currents were observed. In solutions containing rubidium and chloride at pH 8.4–8.8 normally polarized fibres exhibited instantaneous current–voltage relations that were linear over a wide voltage range. Chloride conductance varied widely from fibre to fibre; the mean resting conductance at −80 mV was 7.4 × 10−4 ± 2.6 × 10−4 S/cm2 (mean ± SE). When hyperpolarizing voltage steps were made, conductance declined from the initial to the steady state; inward currents saturated near 14 μA/cm2. In experiments performed on fibres depolarized by immersion in K+-and Rb+-rich solutions it was found that resting conductance did not increase by as much as would be expected from constant field – constant permeability precepts, by comparison with normally polarized fibres. Despite the low chloride transmembrane concentration ratio, rectification in the steady state was similar in depolarized and normally polarized fibres.When a two-pulse protocol was employed to test the availability of chloride conductance after conditioning of the system at some voltage, it was found that the test current, the initial current at the onset of the test voltage step, depended sigmoidally on the conditioning voltage. The sigmoid relationships had asymptotic limits: after hyperpolarizing conditioning the test current was minimal, after depolarizing conditioning, maximal. Normalized sigmoid relations were superimposable, whether from normally polarized or chronically depolarized cells.When the protocol was repeated using different test potentials and initial currents following a particular conditioning voltage were plotted against the test potential, families of straight lines were obtained. The slopes of the members of these families were dependent on the conditioning voltage: the more negative the conditioning step the lower the slope. The lines projected through a mutual intersection at a voltage slightly more positive than the resting potential. This is interpreted as indicating that there is some voltage, slightly positive with respect to the membrane potential, at which the initial current is independent of the conditioning voltage.It is concluded that the state of the chloride conductance mechanism is a function of the deviation of the membrane from the resting potential rather than of the absolute membrane potential and that relaxations from initial to steady states reflect properties of the permeation mechanism rather than accumulation or depletion of chloride in a confined space, although some contribution by a mechanism such as the latter cannot be completely ruled out.

1S,3R-ACPD Induces a Region of Negative Slope Conductance in the Steady-State Current-Voltage Relationship of Hippocampal Pyramidal Cells

1997 ◽  
Vol 77 (1) ◽  
pp. 221-228 ◽  
Author(s):  
Anita Lüthi ◽  
Beat H. Gähwiler ◽  
Urs Gerber
Keyword(s):  
Steady State ◽  
Reversal Potential ◽  
Pyramidal Cells ◽  
Resting Potential ◽  
Negative Slope ◽  
Inward Current ◽  
Current Voltage ◽  
Steady State Current ◽  
Current Voltage Relationship ◽  
Voltage Relationship

Lüthi, Anita, Beat H. Gähwiler, and Urs Gerber. 1 S,3 R-ACPD induces a region of negative slope conductance in the steady-state current-voltage relationship of hippocampal pyramidal cells. J. Neurophysiol. 77: 221–228, 1997. Synaptic responses mediated by metabotropic glutamate receptors (mGluRs) display a marked voltage-dependent increase in amplitude when neurons are moderately depolarized beyond membrane potential. We have investigated the basis for this apparent nonlinear behavior by activatingmGluRs with 1 S,3 R-1-aminocyclopentane-1,3-dicarboxylate(1 S,3 R-ACPD; 10 μM) in CA3 pyramidal cells from rat hippocampal slice cultures with the use of the single-electrode voltage-clamp technique. Under control conditions, cells depolarized from resting potential by 10–20 mV responded with delayed outwardly rectifying currents due to activation of voltage- and Ca2+-dependent K+ conductances. In contrast, in the continuous presence of 1 S,3 R-ACPD, small depolarizations (10–20 mV) induced a delayed inward current. The steady-state current-voltage relationship for this response displayed a region of negative slope conductance at potentials between −55 and −40 mV. The reversal potential of the corresponding 1 S,3 R-ACPD-sensitive tail currents (−93.0 ± 2.2 mV, mean ± SE) was close to the potassium reversal potential, consistent with an mGluR-mediated suppression of K+ current. When external K+ concentration was increased to 8 mM, there was a positive shift in reversal potential to −76.9 ± 5.1 mV. The depolarization-induced inward current in the presence of 1 S,3 R-ACPD was blocked by Ba2+ (1 mM). The response was not dependent on changes in intracellular Ca2+ concentration and was insensitive to bath-applied Cs+ (1 mM), ruling out a contribution of Ca2+-dependent currents or the inward rectifier I Q. Furthermore, the effect of 1 S,3 R-ACPD was not mimicked by inhibiting afterhyperpolarizing current and M current with low-Ca2+ saline (0.5 mM Ca2+, 10 mM Mg2+) containing 10 mM tetraethylammonium chloride. A comparison of the responses induced by 1 S,3 R-ACPD and N-methyl-d-aspartate showed that both induce an inward current with small depolarizations from resting potential but with different kinetics and Mg2+ sensitivity. These results indicate that the suppression of K+ currents in response to activation of mGluRs is markedly voltage dependent, increasing at depolarized potentials and decreasing at hyperpolarized potentials. The negative slope conductance at membrane voltages positive to resting potential may underlie the amplification of mGluR-mediated responses when the membrane potential approaches action potential threshold.

Inward rectification in rat nucleus accumbens neurons

1989 ◽  
Vol 62 (6) ◽  
pp. 1280-1286 ◽  
Author(s):  
N. Uchimura ◽  
E. Cherubini ◽  
R. A. North
Keyword(s):  
Steady State ◽  
Nucleus Accumbens ◽  
Time Course ◽  
Potassium Conductance ◽  
Resting Potential ◽  
Inward Rectification ◽  
Resting Membrane Potential ◽  
Potential Range ◽  
Inward Current ◽  
Rat Nucleus Accumbens

1. Intracellular recordings were made from neurons in slices cut from the rat nucleus accumbens septi. Membrane currents were measured with a single-electrode voltage-clamp amplifier in the potential range -50 to -140 mV. 2. In control conditions (2.5 mM potassium), the resting membrane potential of the neurons was -83.4 +/- 1.1 (SE) mV (n = 157). Steady state membrane conductance was voltage dependent, being 34.8 +/- 1.7 nS (n = 25) at -100 mV and 8.0 +/- 0.7 nS (n = 25) at -60 mV. 3. Barium (1 microM) markedly reduced the inward rectification and caused a small inward current (40.6 +/- 8.7 pA, n = 8) at the resting potential. These effects became larger with higher barium concentrations, and, in 100 microM barium, the current-voltage relation was straight. 4. The block of the inward current by barium (at -130 mV) occurred with an exponential time course; the time constant was approximately 1 s at 1 microM barium and less than 90 ms with 100 microM. Strontium had effects similar to those of barium, but 1000-fold higher concentrations were required. Cesium chloride (2 mM) and rubidium chloride (2 mM) also blocked the inward rectification; their action reached steady state within 50 ms. 5. It is concluded that the nucleus accumbens neurons have a potassium conductance with many features of a typical inward rectifier and that this contributes to the potassium conductance at the resting potential.

Determination of Steady-State Adhesive Wear Rate

2005 ◽  
Author(s):  
L. J. Yang
Keyword(s):  
Steady State ◽  
Wear Rate ◽  
Wear Test ◽  
Standard Test ◽  
Test Method ◽  
Testing Time ◽  
Wear Coefficient ◽  
Wear Rates ◽  
Test Methodology

Wear rates obtained from different investigators could vary significantly due to lack of a standard test method. A test methodology is therefore proposed in this paper to enable the steady-state wear rate to be determined more accurately, consistently, and efficiently. The wear test will be divided into four stages: (i) to conduct the transient wear test; (ii) to predict the steady-state wear coefficient with the required sliding distance based on the transient wear data by using Yang’s second wear coefficient equation; (iii) to conduct confirmation runs to obtain the measured steady-state wear coefficient value; and (iv) to convert the steady-state wear coefficient value into a steady-state wear rate. The proposed methodology is supported by wear data obtained previously on aluminium based matrix composite materials. It is capable of giving more accurate steady-state wear coefficient and wear rate values, as well as saving a lot of testing time and labour, by reducing the number of trial runs required to achieve the steady-state wear condition.

Resting Membrane Properties of Locust Muscle and Their Modulation I. Actions of the Neuropeptides YGGFMRFamide and Proctolin

1998 ◽  
Vol 80 (2) ◽  
pp. 771-784 ◽  
Author(s):  
Christian Walther ◽  
Klaus E. Zittlau ◽  
Harald Murck ◽  
Karlheinz Voigt
Keyword(s):  
Steady State ◽  
Phorbol Esters ◽  
Biological Significance ◽  
Membrane Properties ◽  
Resting Membrane Potential ◽  
Voltage Sensitivity ◽  
Locust Muscle ◽  
Current Voltage ◽  
Instantaneous Current ◽  
Electrode Voltage

Walther, Christian, Klaus E. Zittlau, Harald Murck, and Karlheinz Voigt. Resting membrane properties of locust muscle and their modulation. I. Actions of the neuropeptides YGGFMRFamide and proctolin. J. Neurophysiol. 80: 771–784, 1998. The resting K+ conductance ( G K,r) of locust jumping muscle and its modulation by two neuropeptides, proctolin (Arg-Tyr-Leu-Pro-Thr) and YGGFMRFamide (Tyr-Gly-Gly-Phe-Met-Arg-Phe-NH2), were investigated using the two-electrode voltage clamp. At a physiological [K+]o of 10 mM, G K,r accounts for ∼90% of the membrane resting conductance, and the resting membrane potential differs by ≤1 mV from E K (mean: −74 mV). There is a K+ conductance that slowly activates on hyperpolarization ( G K,H) and that seems to be largely located in the transverse tubules. Steady-state activation of G K,H was analyzed by tail current measurements. G K,H is activated partially at E K but accounts for probably ≤50% of total resting K+ conductance. Raising [K+]o caused a large increase in G K,r and in maximal steady state G K,H without shifting the voltage sensitivity of G K,H. YGGFMRFamide and proctolin reduce G K,H, mainly affecting the maximal steady-state conductance. The voltage-insensitive component of the resting K+ conductance is also reduced. The conductance suppressed by the peptides exhibited an outwardly rectifying instantaneous current/voltage-characteristic that is quite similar to that of G K,H. The actions of the two peptides appeared to be identical, but proctolin was by some two orders of magnitude more potent than YGGFMRFamide. The effects of both peptides are mediated by G proteins. They are mimicked by phorbol esters but do not seem to be initiated by either branch of the phospholipase C-dependent intracellular pathways. The properties of the resting K+ conductance in locust muscle and other invertebrate muscles are compared. The biological significance of peptide-induced reduction in resting K+ conductance is discussed in view of the known property of proctolin to support tonic force as opposed to FMRFamide-peptides that support quick leg movements.

scholarly journals Mathematical Method for Parameters Calculation of electric Characteristic of Photovoltaic Module

2018 ◽  
Vol 3 (4) ◽  
pp. 190-200
Author(s):  
B. Benabdelkrim ◽  
A. Benatillah
Keyword(s):  
Standard Test ◽  
Photovoltaic Module ◽  
Unknown Parameters ◽  
Efficient Manner ◽  
Electric Characteristic ◽  
Precise Knowledge ◽  
Current Voltage ◽  
Pv Modules ◽  
Numerical Approaches ◽  
Parameters Calculation

The study of photovoltaic systems (PV) in an efficient manner requires a precise knowledge of the I-V characteristic curves of PV modules. An accurate current-voltage (I-V) model of PV modules is inherently implicit and non-linear and calls for iterative computations to obtain an analytical expression of current as a function of voltage. In this paper, numerical approaches are proposed to forecast the PV modules performance for engineering applications. The proposed approaches were implemented in a Matlab script and the results have been compared with the datasheet values provided by manufacturers in standard test conditions (STC). These approaches permit to extract the unknown parameters and also allow quantifying the effects of module temperature and irradiance on key cells parameters. In this work, a comparative study of the performance characteristics for different modules thin films and solid is analyzed by a single-diode equivalent circuit using four- and five-parameter models and two diode model.

scholarly journals Ionic Basis of Membrane Potential and of Acetylcholine-evduced Currents in the Cell Body of the Cockroach Fast Coxal Depressor Motor Neurone

1990 ◽  
Vol 151 (1) ◽  
pp. 21-39 ◽  
Author(s):  
JONATHAN A. DAVID ◽  
DAVID B. SATTELLE
Keyword(s):  
Cell Body ◽  
Outward Current ◽  
Motor Neurone ◽  
Resting Potential ◽  
Inward Current ◽  
Current Voltage ◽  
Low K ◽  
Current Voltage Relationship ◽  
Ionic Basis ◽  
Voltage Relationship

The ionic basis of the resting potential and of the response to acetylcholine (ACh) has been investigated in the cell body membrane of the fast coxal depressor motor neurone in the metathoracic ganglion of the cockroach Periplaneta americana. By means of ion-sensitive microelectrodes, intracellular concentrations of three ion species were estimated (mmoll−1): [K+]i, 1443; [Na+]i, 9±1; [Cl−], 7±1. The resting potential of continuously superfused cells was −75.6±1.9mV at 22° C. A change in resting potential of 42.0±2.5mV accompanied a decade change in [K+]o. Experiments with (10−4moll−1) ouabain, Na+ injection, low temperature (10°C) and non-superfused cells indicated the presence of an electrogenic sodium pump. Under current-clamp, the cell body membrane was depolarized by sequentially applied, ionophoretic pulses (500ms duration) of ACh. Under voltage-clamp, such doses of ACh resulted in an inward current which was abolished in low-Na+ saline. Ion-sensitive electrodes revealed an increase in [Na+]i but no change in [Cl−1]j in response to externally applied ACh. The ACh-induced current-voltage relationship was shifted in a negative direction by low-K+ saline. The AChinduced inward current was usually followed by a delayed outward current which reversed at Ek. Low-K+ saline had the same effect on this outward component as depolarizing the membrane. This suggests that the outward current component is carried by K+. The ACh-induced inward current and the delayed outward current were potentiated either when [Ca2+]i was lowered by injecting the calcium chelator BAPTA or by exposure of the cell to low-Ca2+ saline. High-Ca2+ saline reduced the inward component of the response and produced a negative shift in the AChinduced current-voltage relationship. The amplitude of the delayed outward

scholarly journals Modeling of Photovoltaic Module Using the MATLAB

2019 ◽  
Vol 9 ◽  
pp. 59-69
Author(s):  
Alok Dhaundiyal ◽  
Divine Atsu
Keyword(s):  
Standard Test ◽  
Electrical Performance ◽  
Photovoltaic Module ◽  
Solar Pv ◽  
Pv Module ◽  
Proposed Model ◽  
Current Voltage ◽  
Pv Modules ◽  
The Impact ◽  
The Mathematical Model

This paper presents the modeling and simulation of the characteristics and electrical performance of photovoltaic (PV) solar modules. Genetic coding is applied to obtain the optimized values of parameters within the constraint limit using the software MATLAB. A single diode model is proposed, considering the series and shunt resistances, to study the impact of solar irradiance and temperature on the power-voltage (P-V) and current-voltage (I-V) characteristics and predict the output of solar PV modules. The validation of the model under the standard test conditions (STC) and different values of temperature and insolation is performed, as well as an evaluation using experimentally obtained data from outdoor operating PV modules. The obtained results are also subjected to comply with the manufacturer’s data to ensure that the proposed model does not violate the prescribed tolerance range. The range of variation in current and voltage lies in the domain of 8.21 – 8.5 A and 22 – 23 V, respectively; while the predicted solutions for current and voltage vary from 8.28 – 8.68 A and 23.79 – 24.44 V, respectively. The measured experimental power of the PV module estimated to be 148 – 152 W is predicted from the mathematical model and the obtained values of simulated solution are in the domain of 149 – 157 W. The proposed scheme was found to be very effective at determining the influence of input factors on the modules, which is difficult to determine through experimental means.

Electrophysiological Investigations of the Heart of Squilla Mantis

1964 ◽  
Vol 41 (4) ◽  
pp. 701-722
Author(s):  
HILARY F. BROWN
Keyword(s):  
Heart Muscle ◽  
Vital Staining ◽  
Heart Beat ◽  
Nerve Endings ◽  
Resting Potential ◽  
Muscle Membrane ◽  
Absolute Level ◽  
Local Contraction ◽  
Set Up ◽  
Junction Potential

1. The histology of the heart muscle of Squilla mantis is briefly described. Vital staining with methylene blue revealed only a sparse distribution of nerve endings on the muscle network. 2. Intracellular electrodes recorded from the muscle a multi-peaked junction potential at each heart beat. Each peak followed an impulse in the ganglionic nerve trunk burst. All the peaks were approximately the same height and none more than about three-fifths the height of the resting potential (average values for 10 hearts: resting potential, 51.5 mV; junction potential, 27.6 mV). 3. Inverted (negative-going) signals were never recorded just outside the muscle membrane suggesting that at all the points which were searched the membrane was acting passively. 4. Driving Squilla heart muscle via its nerve supply at 100 stimuli per second did not depolarize it by more than about 35 mV, nor would depolarizing pulses given directly to a fibre through an intracellular electrode set up any sort of current-generating activity in the membrane. 5. The magnitude of muscle contraction, measured locally using a microelectrode transducer, depended on the absolute level of the potential across the membrane, rather than on change of potential. 6. A directly applied electrotonus, similar in magnitude and duration to the nerve-induced junction potential, caused a local contraction of similar magnitude. 7. The recorded junction potential is therefore interpreted as the composite record of the electrotonus spreading within the muscle network from current initiated at relatively infrequent active points on the muscle membrane (the nerve endings) which passively depolarizes the rest of the membrane. 8. The junction potentials showed facilitation when the intervals between them were below 4 sec. At intervals less than 630 msec, they summed.

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