Further identification of multiple responses mediated by dopamine in the CNS of Planorbis corneus

1978 ◽  
Vol 56 (1) ◽  
pp. 7-18 ◽  
Author(s):  
J. F. MacDonald ◽  
M. S. Berry
Keyword(s):  
Neuroleptic Drugs ◽  
Intracellular Recordings ◽  
Excitatory Postsynaptic Potentials ◽  
Multiple Responses ◽  
Gastropod Mollusc ◽  
Postsynaptic Potentials ◽  
Synaptic Potentials ◽  
Inhibitory Postsynaptic Potentials ◽  
Planorbis Corneus ◽  
Synaptic Responses

Intracellular recordings from neurones, receiving monosynaptic contacts from a dopamine-containing (DA-containing) neurone in the central ganglia of the gastropod mollusc Planorbis corneus, revealed that there are at least three DA-mediated responses. These are 'fast' excitatory postsynaptic potentials (EfPSPs) (200 ms), 'slow' excitatory postsynaptic potentials (EsPSPs) (900 ms), and inhibitory postsynaptic potentials (IPSPs) (200–900 ms). Various combinations of these synaptic potentials were recorded from postsynaptic neurones: EsPSPs, EfPEPs, EsEfPSPs, or EfIPSPs. Neurones receiving such connections also responded appropriately to iontophoresized DA with a 'fast' depolarization (EfPSPs), a 'slow' depolarization (EsPSPs), or a hyperpolarization (IPSPs). These responses could be distinguished on the basis of function (excitation or inhibition), duration, rate of desensitization, and sensitivity to apomorphine, D-LSD, and tubocurarine. The neuroleptic drugs (DA antagonists) haloperidol, fluphenazine, and metoclopramide reduced both excitatory and inhibitory DA transmission. This investigation strongly supports the hypothesis that DA is the transmitter mediating multiple synaptic responses in Planorbis.

Histaminergic synaptic transmission in the cerebral ganglion of Aplysia

1985 ◽  
Vol 53 (4) ◽  
pp. 1016-1037 ◽  
Author(s):  
R. E. McCaman ◽  
D. Weinreich
Keyword(s):  
Action Potential ◽  
Synaptic Transmission ◽  
Voltage Clamp ◽  
Cerebral Ganglion ◽  
Major Influence ◽  
Electrical Synapses ◽  
Postsynaptic Potentials ◽  
Synaptic Potentials ◽  
Chemical Synapses ◽  
Synaptic Responses

Standard intracellular stimulating and recording techniques including voltage-clamp were used to analyze the synaptic responses mediated by two identifiable histamine-containing neurons (HCNs), designated C2 neurons, located in bilaterally symmetric clusters of the isolated cerebral ganglion of Aplysia california. Activation of each C2 induced unitary chemically mediated synaptic potentials in over 15 identified ipsilateral follower neurons. Several additional followers were connected to the HCNs by nonrectifying electrical synapses. Most of the follower neurons examined received only chemical synapses from the C2s. Some of the followers were reciprocally connected with each other through nonrectifying electrical synapses. A single C2 action potential can evoke six distinctive types of chemically mediated postsynaptic potentials (PSPs) in different follower neurons. Most of the PSPs have been shown to be multicomponent, i.e., they are comprised of various combinations of individual fast (less than or equal to 150 ms), slow (1-2 s), and very slow (greater than or equal to 4 s) depolarizing and hyperpolarizing components. The combination of these components produces PSPs of varying complexity, from simple monophasic responses such as the frequently observed slow excitatory PSPs and slow inhibitory PSPs to responses consisting of two to three components such as fast excitatory, slow inhibitory PSPs or fast inhibitory, slow excitatory PSPs. All of the multicomponent PSPs appear to be mediated through monosynaptic connections from the C2, as determined by various electrophysiological criteria. The slow and very slow synaptic components of the multicomponent PSPs were markedly potentiated in amplitude and duration after repetitive C2 activation. This property of the slow components permits the slower PSPs to exert a major influence on the excitability and integrative properties of the follower neurons.

Duodenal neurons provide nicotinic fast synaptic input to sphincter of Oddi neurons in guinea pig

1999 ◽  
Vol 277 (1) ◽  
pp. G226-G234 ◽  
Author(s):  
Gary M. Mawe ◽  
Audra L. Kennedy
Keyword(s):  
Synaptic Input ◽  
Sphincter Of Oddi ◽  
Duodenal Mucosa ◽  
Postsynaptic Potentials ◽  
Chemical Coding ◽  
Neural Connections ◽  
Inhibitory Postsynaptic Potentials ◽  
Neural Communication ◽  
Antidromic Responses ◽  
Synaptic Responses

We have investigated the existence of neural connections between the duodenum and the sphincter of Oddi (SO). Stimulation of duodenal myenteric fiber bundles elicited synaptic responses in SO neurons, which included nicotinic fast excitatory postsynaptic potentials (EPSPs), slow EPSPs, and α2-adrenoreceptor-mediated inhibitory postsynaptic potentials. After 48 h in organ culture, when extrinsic fibers had diminished, only the fast EPSPs persisted. Duodenal mucosal stimulation also elicited nicotinic fast EPSPs in SO neurons. There was no association between the SO neurons that received duodenal input and their chemical coding. A reciprocal projection also exists from the SO to the duodenum. In acute and cultured preparations, duodenal myenteric stimulation caused antidromic responses in 20% of SO neurons. Furthermore, 45.6 ± 10.5 neurons in SO ganglia were retrogradely labeled from dye application sites in the duodenum. It is proposed that bidirectional neural communication occurs between the duodenum and the SO and that duodenal neurons provide excitatory fast synaptic input to SO neurons through a reflex that can be activated at the duodenal mucosa.

Differential responsiveness of neocortical areas in the parieto-occipital region to low-intensity stimulation in vitro

1996 ◽  
Vol 76 (1) ◽  
pp. 622-625 ◽  
Author(s):  
T. Neumann-Haefelin ◽  
G. Hagemann ◽  
O. W. Witte
Keyword(s):  
Extracellular Recording ◽  
The Other ◽  
Electrophysiological Recording ◽  
Late Response ◽  
Occipital Region ◽  
Intracellular Recordings ◽  
Excitatory Postsynaptic Potentials ◽  
Postsynaptic Potentials ◽  
Low Intensity

1. Extracellular recording techniques were used to map the responses to low-intensity stimuli across rat neocortical slices prepared from the parieto-occipital region. Intracellular recordings were obtained in regions of particular interest. After electrophysiological recording, slices were fixed in paraformaldehyde (4%) and Nissl-stained to allow identification of cytoarchitectonically discrete areas. 2. In most neocortical areas of the parieto-occipital region (Par1, HL, Fr1, Fr2), low-intensity stimuli applied to layer V evoked only short monophasic field excitatory postsynaptic potentials (fEPSPs) in layer II/III. In Oc2 (secondary visual cortex, rostral part), however, a long-lasting potential (up to 450 ms) consistently followed the short monophasic response seen in the other areas. 3. The late response observed in Oc2 was characterized by its inter-stimulus variability, polyphasic appearance and N-methyl-D-aspartate (NMDA)-receptor dependence. Intracellular recordings obtained from layer II/III pyramidal neurons located in Oc2 revealed short-latency, monophasic early excitatory postsynaptic potentials (eEPSPs) followed by long-lasting excitatory postsynaptic potentials (eEPSPs) followed by long-lasting excitatory postsynaptic potentials (EPSPs), which often triggered several action potentials 4. Recordings with two extracellular electrodes, one positioned directly above the stimulation electrode and the other at a variable distance (but also in layer II/III), suggested that the corresponding excitation spreads across the slice, but does not cross the cytoarchitectonic borders of Oc2. 5. Our data indicate that the electrophysiological responses to low-intensity stimuli differ within the parieto-occipital region of the rat neocortex. The type of response elicited depends on the cytoarchitectonic area in which the stimulus is applied. The results raise the question of whether further differences between neocortical subregions, with respect to basic electrophysiological properties, exist.

Cerebral Interneurones Controlling Feeding Motor Output In The Snail Lymnaea Stagnalis

1989 ◽  
Vol 147 (1) ◽  
pp. 361-374
Author(s):  
CATHERINE R. McCROHAN ◽  
MICHAEL A. KYRIAKIDES
Keyword(s):  
Central Pattern Generator ◽  
Lymnaea Stagnalis ◽  
Pattern Generator ◽  
Motor Output ◽  
Excitatory Postsynaptic Potentials ◽  
Gastropod Species ◽  
Postsynaptic Potentials ◽  
Inhibitory Postsynaptic Potentials ◽  
Buccal Ganglia

1. The cerebral ventral 1 (CV1) interneurones of Lymnaea occurred as a population of at least three in each ganglion, all with similar morphologies. Steady depolarization of a CV1 cell led to initiation and maintenance of rhythmic feeding motor output from the buccal ganglia. 2. CV1 interneurones produced facilitating excitatory postsynaptic potentials in Nl interneurones of the buccal central pattern generator for feeding. Connections with N2 interneurones were not found. 3. The CV1 population could be separated into two subgroups. CVla received strong synaptic feedback in phase with the buccal rhythm, leading to strong bursting during generation of feeding motor output. CVlb received only weak feedback, and often fired continuously when depolarized. 4. Unitary inhibitory postsynaptic potentials were characteristic of all CV1 neurones, but were only visible in CVlb when it was depolarized. These inputs are thought to arise indirectly from the buccal central pattern generator. 5. The CV1 population is probably homologous with similar neurones in other gastropod species.

Synaptic mechanisms in sympathetic preganglionic neurons

1992 ◽  
Vol 70 (S1) ◽  
pp. S86-S91 ◽  
Author(s):  
N. J. Dun ◽  
S. Y. Wu ◽  
E. Shen ◽  
T. Miyazaki ◽  
S. L. Dun ◽  
...  
Keyword(s):  
Excitatory Amino Acid ◽  
Neonatal Rat ◽  
Excitatory Postsynaptic Potentials ◽  
Sympathetic Preganglionic Neurons ◽  
Postsynaptic Potentials ◽  
Preganglionic Neurons ◽  
Inhibitory Postsynaptic Potentials ◽  
Ventral Roots ◽  
Adult Cat ◽  
Synaptic Mechanisms

Intracellular recordings from sympathetic preganglionic neurons (SPNs) in adult cat and neonatal rat spinal cord slices reveal four types of synaptic potentials, namely, excitatory postsynaptic potentials (EPSPs), inhibitory postsynaptic potentials (IPSPs), and slow EPSPs in both preparations, and a slow IPSP in cat SPNs. Pharmacological studies show that glutamate or a related excitatory amino acid and glycine are the probable mediators of EPSPs and IPSPs. There may be heterogenous mediators of slow EPSPs; substance P, serotonin, norepinephrine, and epinephrine are all probable mediators of slow EPSPs in subpopulations of SPNs. In the case of slow IPSPs, norepinephrine appears to be the likely transmitter. Finally, stimulation of ventral roots elicits a synaptic potential that appears to be caused by glutamate released from afferent fibers in the ventral roots. Our results indicate that a multitude of synaptic mechanisms exist in the rat SPNs by means of which inputs arising from sensory and supraspinal neurons are processed in a timely and orderly manner, thus ensuring highly organized but differentiated outputs to multiple peripheral target cells.Key words: sympathetic preganglionic neurons, excitatory postsynaptic potentials, inhibitory postsynaptic potentials, slow excitatory postsynaptic potentials, glutamate, glycine.

Anoxic depression of excitatory and inhibitory postsynaptic potentials in rat neocortical slices

1993 ◽  
Vol 69 (1) ◽  
pp. 109-117 ◽  
Author(s):  
A. S. Rosen ◽  
M. E. Morris
Keyword(s):  
White Matter ◽  
Adenosine Triphosphatase ◽  
Pyramidal Neurons ◽  
Transient Increase ◽  
Differential Sensitivity ◽  
Epsp Amplitude ◽  
Postsynaptic Potentials ◽  
Synaptic Potentials ◽  
Inhibitory Postsynaptic Potentials ◽  
Higher Sensitivity

1. The effects of brief anoxia (4-6 min replacement of O2 by N2) on synaptic potentials evoked from layer IV and/or the white matter were studied in pyramidal neurons of layers II-III from rat neocortical slices. 2. The early and late components of excitatory postsynaptic potentials (EPSPs) showed differential sensitivity to anoxia: within 2 min the late EPSP (lEPSP) disappeared, whereas the amplitude of the early EPSP (eEPSP) decreased by 70% at 5 min of anoxia. Recovery was complete within 4–11 min. 3. Both fast and slow inhibitory postsynaptic potentials (IPSPs) were extremely sensitive to lack of O2 and were abolished earlier than the lEPSP evoked by the same stimulus. As well, recovery of the IPSPs was always more delayed than that of the EPSPs. 4. A transient increase in excitability during early anoxia and/or midrecovery, manifested as enhanced probability of spiking in 25% of neurons, is attributed to the higher sensitivity of IPSPs compared with EPSPs. 5. The anoxic-induced depression of the lEPSP and IPSPs, which are generated close to the soma, is not due to depolarization-induced occlusion; however, occlusion may cause an attenuation of the eEPSP at dendritic sites. 6. The depression of the EPSPs is not a result of a decreased transmembrane Na+ gradient after inactivation of Na-K-adenosine triphosphatase (Na-K-ATPase). Although ouabain induced a depolarization similar to that of anoxia, it did not affect EPSP amplitude.(ABSTRACT TRUNCATED AT 250 WORDS)

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