Mean velocity of optically detected intraaxonal particles measured by a cross-correlation method

1976 ◽  
Vol 54 (6) ◽  
pp. 859-869 ◽  
Author(s):  
R. S. Smith ◽  
Z. J. Koles
Keyword(s):  
Cross Correlation ◽  
Correlation Method ◽  
Nerve Fibers ◽  
Mean Velocity ◽  
Myelinated Nerve ◽  
Optical Signals ◽  
Accuracy And Precision ◽  
Small Collection ◽  
The Mean ◽  
Optically Detected

A method which uses the cross correlation of optical signals is described for the determination of the mean velocity of somatopetally moving particles within nerve fibers. The method was validated by simulation experiments and by comparing the results with those obtained by averaging collections of velocities of individual particles. The significant contribution of the method is that it allows objective and rapid serial evaluations of mean particle velocity within individual nerve fibers with good accuracy and precision. A series of results from normal myelinated nerve fibers from Xenopus laevis is presented. Considerable variation (up to 50%) in mean velocity was found between individual nerve fibers. The mean of all determinations indicates that the mean velocity of somatopetally moving particles in axons with diameters > 10 μm is in the region of 1.14 μm/s at a temperature of 22–24 °C. The findings are compared with the small collection of such determinations which have been reported in the literature.

Cross-Correlation Velocimetry for Measurement of Velocity and Temperature Profiles in Low-Speed, Turbulent, Nonisothermal Flows

1992 ◽  
Vol 114 (2) ◽  
pp. 331-337 ◽  
Author(s):  
V. Motevalli ◽  
C. H. Marks ◽  
B. J. McCaffrey
Keyword(s):  
Cross Correlation ◽  
Temperature Profiles ◽  
Fire Plume ◽  
Mean Velocity ◽  
Low Speed ◽  
Nonisothermal Flows ◽  
Degree Of Confidence ◽  
The Mean ◽  
Temperature Time ◽  
Better Than

A technique utilizing thermocouple pairs as sensors to measure velocity and temperature profiles in low-speed, turbulent, nonisothermal flows is described here. In this technique, Cross-Correlation Velocimetry (CCV), the temperature-time records from a pair of thermocouples, one downstream of the other, are cross-correlated to determine the flow’s preferred mean velocity while temperature is measured directly. The velocity measurements have undergone extensive verification using hotwire, pitot tube, and Laser-Doppler Velocimetry to determine the degree of confidence in this technique. This work demonstrates that the CCV technique is quite reliable and can measure the mean preferred component of the convective velocity with better than ±5 percent certainty. Application of this technique to the measurement of velocities in a ceiling jet induced by a fire plume is briefly presented here.

The determination of the instantaneous velocity of axonally transported organelles from filmed records of their motion

1982 ◽  
Vol 60 (5) ◽  
pp. 670-679 ◽  
Author(s):  
K. J. Koles ◽  
K. D. McLeod ◽  
R. S. Smith
Keyword(s):  
Power Spectra ◽  
Computational Procedure ◽  
Dark Field ◽  
Nerve Fibers ◽  
Low Noise ◽  
Instantaneous Velocity ◽  
Mean Velocity ◽  
Myelinated Nerve ◽  
Motion Picture Film ◽  
Band Limited

A computational procedure is described for obtaining reproducible, low noise estimates of the instantaneous velocity of axonally transported organelles. Axonally transported organelles were detected in myelinated nerve fibers from Xenopus laevis by dark-field microscopy. The motion of the organelles was recorded on motion picture film at 3 frames/s, and the position of organelles travelling in the retrograde direction was obtained as a pair of x (axial) and y (transverse) coordinates at each 0.33-s interval. The trend in organelle movement with time was calculated for each of the series of x and y coordinates by linear regression. This trend was removed from the measurements of x and y to yield sets of trend-free displacements. The trend yielded a measure of the mean velocity of the organelle in each of the two orthogonal directions. Power spectra of the deviations in x and y about the trend were calculated. For 133 particles studied, 99% of the power in the trend-free deviations occurred at frequencies below 0.3 Hz. The peak power in the x and y deviations occurred at a frequency of 0.1 Hz or less. Positional deviations about the trend were treated with a discrete 21-term differentiating filter that attenuated frequencies above 0.3 Hz. Instantaneous velocities for the organelles were obtained by adding the result of the band-limited differentiation to the appropriate estimates of mean velocity. The 21-term method was compared with a commonly used 2-term approximation to a differentiator and was shown to produce velocity estimates with about one order of magnitude less error. Estimates of organelle velocity obtained with the 21-term method indicate that saltatory particle motion may be viewed either as a smooth variation of particle velocity with respect to time or as an irregular, or discontinuous, variation of velocity with respect to particle position.

scholarly journals Measurements of debris flow velocity through cross-correlation of instrumentation data

2005 ◽  
Vol 5 (1) ◽  
pp. 137-142 ◽  
Author(s):  
M. Arattano ◽  
L. Marchi
Keyword(s):  
Debris Flow ◽  
Cross Correlation ◽  
Time Lag ◽  
Ground Vibration ◽  
Front Velocity ◽  
Correlation Technique ◽  
Ultrasonic Sensors ◽  
Mean Velocity ◽  
Different Types ◽  
The Mean

Abstract. Detection of debris flow occurrence can be efficiently obtained through different types of sensors. A pair of ultrasonic sensors placed at a known distance from each other along a torrent have been used as a method to obtain mean front velocity of debris-flows, in addition to their use as detectors of debris flow occurrence. Also seismic and acoustic sensors have been employed to measure debris-flow front velocity and discharge in the same manner. In order to obtain velocity measurements, however, these methods require the presence of a well identifiable and defined main front in the debris flow wave. The time lag between the recordings of the front of the wave at two consecutive stations allows an estimation of its mean velocity. When a well-defined front is not present and no recurrent feature can be found along the wave, the measurement of velocity may prove difficult. The cross-correlation technique may help identifying the mean velocity of the flow in such cases. In fact, cross correlation allows to determine the mean time lag elapsed between the recording of two sets of data of the same event at different positions. This technique may be also used to measure velocity using signals coming from different types of sensors, for instance where a ground vibration detector has been placed along a torrent where an ultrasonic sensor was already present or viceversa. An application has been made using field data recorded through seismic and ultrasonic sensors in a small instrumented catchment in the Italian Alps (Moscardo Torrent).

A New Sensor for On-Line Monitoring the Temperature and Velocity of Thermal Spray Particles

2000 ◽  
Author(s):  
G. Bourque ◽  
M. Lamontagne ◽  
C. Moreau
Keyword(s):  
Time Delay ◽  
Thermal Spray ◽  
Optical Fibers ◽  
Cross Correlation ◽  
Mean Velocity ◽  
Mean Temperature ◽  
On Line ◽  
Band Pass ◽  
The Mean ◽  
Thermal Spray Processes

Abstract In this paper, we describe a new sensor for monitoring inflight particles in thermal spray processes. The sensor can measure simultaneously and in real-time, the mean velocity and mean temperature of the particle jet for a very broad range of powder feed rates. The thermal radiation emitted by the hot particles is collected by a lens and focused on two optical fibers. Knowing the distance between the optical fibers and the magnification of the optics, the mean particle velocity is computed by measuring the time delay between the signals collected in the two fibers by cross-correlation. The signals are band-pass filtered to prevent spurious reflection, equipment movement and noise from disturbing the measurement. Using the same signals filtered at two specific wavelengths, the mean temperature of the particle jet is obtained by the two-color pyrometry technique. In this technique, the temperature is computed from the ratio of the light intensity detected at two different wavelengths.

Application of cross correlation to HREM images of surfaces

1987 ◽  
Vol 45 ◽  
pp. 754-755
Author(s):  
D. E. Luzzi ◽  
L. D. Marks ◽  
M. I. Buckett
Keyword(s):  
Cross Correlation ◽  
A Priori ◽  
Matrix Material ◽  
Correlation Method ◽  
Accurate Knowledge ◽  
Complex Image ◽  
Fourier Filtering ◽  
The Matrix ◽  
Low Pass ◽  
Data Reduction Technique

As the HREM becomes increasingly used for the study of dynamic localized phenomena, the development of techniques to recover the desired information from a real image is important. Often, the important features are not strongly scattering in comparison to the matrix material in addition to being masked by statistical and amorphous noise. The desired information will usually involve the accurate knowledge of the position and intensity of the contrast. In order to decipher the desired information from a complex image, cross-correlation (xcf) techniques can be utilized. Unlike other image processing methods which rely on data massaging (e.g. high/low pass filtering or Fourier filtering), the cross-correlation method is a rigorous data reduction technique with no a priori assumptions.We have examined basic cross-correlation procedures using images of discrete gaussian peaks and have developed an iterative procedure to greatly enhance the capabilities of these techniques when the contrast from the peaks overlap.

Cross-Correlation Method for the Formation of Laser Energy Fields with Complex Distributions

2013 ◽  
Vol 58 (2) ◽  
pp. 122-125 ◽  
Author(s):  
O.V. Gnatovskyy ◽  
◽  
A.M. Negriyko ◽  
V.O. Gnatovskyy ◽  
A.V. Sidorenko ◽  
...  
Keyword(s):  
Cross Correlation ◽  
Laser Energy ◽  
Correlation Method

HISTOMETRIC AND ULTRASTRUCTURAL ORGANIZATION OF THE NERVIMUSCULAR TERMINALS OF SKELETAL MUSCLES AT A HYPOKINESIA

2019 ◽  
pp. 55-63
Author(s):  
Z. M. Yaschyshyn ◽  
S. L. Popel
Keyword(s):  
Skeletal Muscles ◽  
Structural Adjustment ◽  
Muscle Fibers ◽  
Electron Microscopic Examination ◽  
Nerve Fibers ◽  
Ultrastructural Changes ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Myelinated Nerve ◽  
Male Wistar Rats

The aim: to study the dynamics of histological and ultrastructural changes in muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia at different stages of ontogenesis. Methods. Studied skeletal muscles and their peripheral nervous apparatus of laboratory male Wistar rats aged 30 to 270 days. The restriction of motor activity was carried out in special canister cells for 30, 60, 90, and 240 days (5 animals for each term). To determine the type of muscle fiber, the Nahlas histochemical method was used, the Kulchitsky method was used to detect myelinated nerve fibers, the Bilshovsky-Gros method and the electron microscopic method to identify neuromuscular endings. Results. The data of histological and electron microscopic examination of skeletal muscle fibers and their neuromuscular endings under conditions of prolonged hypokinesia indicate their regular restructuring during the development of muscles, the formation of their synapses and structures that are associated with them at different stages of ontogenesis. Conclusion. The study provides an in-depth understanding of the relative frequency and nature of the disturbance of the neuromuscular endings during prolonged hypokinesia and its effect on the dynamics of structural adjustment of individual types of muscle fibers in ontogenesis.

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