Regulation of glucagon-stimulated production of glucose in rat liver by guanosine 3′,5′-cyclic phosphate

Richard M. Epand; Connie Prosser

doi:10.1139/y76-116

Regulation of glucagon-stimulated production of glucose in rat liver by guanosine 3′,5′-cyclic phosphate

Canadian Journal of Physiology and Pharmacology ◽

10.1139/y76-116 ◽

1976 ◽

Vol 54 (6) ◽

pp. 834-837 ◽

Cited By ~ 2

Author(s):

Richard M. Epand ◽

Connie Prosser

Keyword(s):

Rat Liver ◽

Guanylate Cyclase ◽

Glucose Production ◽

Liver Slices ◽

Cyclic Phosphate ◽

Camp And Cgmp

Exogenous cGMP can inhibit both basal and glucagon-stimulated production of glucose in liver slices from fed rats. Thus, cAMP and cGMP have opposite effects on the production of glucose in rat liver. Acetylcholine, an activator of guanylate cyclase (EC 4.6.1.2) in other systems, also inhibits the glucagon-stimulated production of glucose. No effect on glucose production was observed with secretin or exogenous GTP.

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OXYDATION DES ANABOLIKUMS 1-METHYL-ANDROST-1-EN-17β-OL-3-ON MIT WASSERSTOFFTRANSFER AUF ÖSTRON

Acta Endocrinologica ◽

10.1530/acta.0.0670517 ◽

1971 ◽

Vol 67 (3) ◽

pp. 517-530 ◽

Cited By ~ 1

Author(s):

Martin Wenzel

Keyword(s):

Rat Liver ◽

Anabolic Steroid ◽

Female Rats ◽

Male Rats ◽

Female Rat ◽

Liver Slices ◽

Androgen Effects ◽

Biochemical Difference

ABSTRACT With the aid of metenolon-17α-T a tritium-transfer to oestrone in rat liver slices was demonstrated. This tritium-transfer from metenolon17α-T to oestrone yielding tritium-labelled oestradiol had a higher efficiency in male than in female rat liver. Correspondingly in the presence of metenolon the relation of oestrone to oestradiol is changed more in male than in female rat liver. Looking for biochemical differences between the anabolic steroid metenolon and testosterone the oxydation at C17 was measured in different organs of the rat using 17α-T-labelled steroids. The highest oxydation rate was found for both steroids in the liver. In the sexual organs of male rats the oxydation rate of testosterone was 50–10 times higher than that of the anabolic steroid. This difference was less in sexual organs of female rats. This result of a greater biochemical difference between both steroids in males than in females leads to the question, whether the dissociation between the anabolic and the androgen effects is higher in males than in females.

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THE ESTIMATION OF FATTY ACID SYNTHESIS IN RAT LIVER SLICES

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)77265-2 ◽

1953 ◽

Vol 205 (1) ◽

pp. 401-408

Author(s):

Grace Medes ◽

Morris A. Spirtes ◽

Sidney Weinhouse

Keyword(s):

Fatty Acid ◽

Rat Liver ◽

Fatty Acid Synthesis ◽

Acid Synthesis ◽

Liver Slices

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Influence of Slice Thickness and Culture Conditions on the Metabolism of 7-Ethoxycoumarin in Precision-cut Rat Liver Slices

Alternatives to Laboratory Animals ◽

10.1177/026119299802600417 ◽

1998 ◽

Vol 26 (4) ◽

pp. 541-548

Author(s):

Roger J. Price ◽

Anthony B. Renwick ◽

Paula T. Barton ◽

J. Brian Houston ◽

Brian G. Lake

Keyword(s):

Gas Phase ◽

Rat Liver ◽

Xenobiotic Metabolism ◽

Culture Conditions ◽

Slice Thickness ◽

Dependent Manner ◽

Sprague Dawley ◽

Liver Slices ◽

Sprague Dawley Rats ◽

Rat Livers

This study investigated the effects of some experimental variables on the rate of xenobiotic metabolism in precision-cut rat liver slices. Liver slices of 123 ± 8μm (mean ± SEM of six slices), 165 ± 3μm, 238 ± 6μm and 515 ± 14μm thickness were prepared from male Sprague-Dawley rats, and incubated in RPMI 1640 medium in an atmosphere of 95% O2/5% CO2 by using a dynamic organ culture system. Liver slices of all thicknesses metabolised 10μM 7-ethoxycoumarin to total (free and conjugated) 7-hydroxycoumarin in a time-dependent manner. The rate of 7-ethoxycoumarin metabolism was greatest in 165μm thick slices and slowest in 515μm thick slices, being 2.74 ± 0.19pmol/minute/mg slice protein and 0.69 ± 0.07pmol/minute/mg slice protein, respectively. No marked effects on the rate of 7-ethoxycoumarin metabolism in liver slices were observed either by changing the medium to Earle's balanced salt solution (EBSS) or by changing the gas phase to 95% air/5% CO2. Moreover, the perfusion of rat livers with EBSS at 2–4°C, prior to preparation of tissue cores, did not enhance 7-ethoxycoumarin metabolism in rat liver slices. In this study, the optimal slice thickness was 175μm, with higher rates of 7-ethoxycoumarin metabolism being observed than with 250μm thick slices, which are often used for studies of xenobiotic metabolism. Variable results were obtained with slices of around 100–120μm thickness, which may be attributable to the ratio between intact hepatocytes and cells damaged by the slicing procedure in these very thin slices.

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