Bile Acid Metabolism in Mammals. VIII. Biliary Secretion of Cholylarginine by the Isolated Perfused Rat Liver

1975 ◽  
Vol 53 (5) ◽  
pp. 880-887 ◽  
Author(s):  
I. M. Yousef ◽  
M. M. Fisher
Keyword(s):  
Cholic Acid ◽  
Rat Liver ◽  
Acid Metabolism ◽  
Apparent Molecular Weight ◽  
Bile Acid Metabolism ◽  
Isolated Perfused Rat Liver ◽  
Spectrometric Analysis ◽  
Gas Liquid Chromatography ◽  
Perfused Rat Liver ◽  
Perfusion Studies

In studies of cholic acid metabolism using the isolated perfused rat liver system, an unknown conjugate of cholic acid was observed. This conjugate comprised 15–27% of the biliary bile acids in these experiments, was less polar than cholylglycine on thin-layer chromatography using butanol, acetic acid, and water, and had an apparent molecular weight greater than that of cholyltaurine on gas–liquid chromatography. Amino acid analysis of the hydrolyzed conjugate demonstrated the presence of arginine. Perfusion studies with radioactive arginine, and mass spectrometric analysis proved that the conjugate was cholylarginine. Secretion of this conjugate does not represent a deficiency of available glycine and taurine.

Amino acid metabolism in isolated perfused rat liver

1990 ◽  
Vol 49 (1) ◽  
pp. 8-13 ◽  
Author(s):  
J.P. De Bandt ◽  
L. Cynober ◽  
F. Ballet ◽  
C. Coudray-Lucas ◽  
C. Rey ◽  
...  
Keyword(s):  
Amino Acid ◽  
Rat Liver ◽  
Amino Acid Metabolism ◽  
Acid Metabolism ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver

Bile Acid Metabolism in Mammals. V. Studies on the Sex Difference in the Response of the Isolated Perfused Rat Liver to Chenodeoxycholic Acid

1973 ◽  
Vol 51 (6) ◽  
pp. 418-423 ◽  
Author(s):  
I. M. Yousef ◽  
R. Magnusson ◽  
V. M. Price ◽  
M. M. Fisher
Keyword(s):  
Bile Acid ◽  
Sex Difference ◽  
Rat Liver ◽  
Chenodeoxycholic Acid ◽  
Bile Acid Metabolism ◽  
Isolated Perfused Rat Liver ◽  
Base Line ◽  
Female Rat ◽  
Perfused Rat Liver ◽  
Perfusion Medium

The hepatic metabolism of chenodeoxycholic acid (CDCA) was studied using the isolated perfused rat liver technique. In 12 perfusions, six male and six female, 30 μmol of CDCA were added to the perfusion medium, and in 12 other perfusions, also six of each sex, 1 μmol of CDCA was added to the perfusion medium. The CDCA was added after 2 h of base-line perfusion and the bile acids of liver, plasma, and bile were analyzed by combined thin-layer and gas chromatography. In the 2 h of perfusion prior to the addition of exogenous CDCA there were sex differences in the kinetics of bile acid secretion in the bile and in the bile acid composition of that bile. Following the addition of CDCA to the perfusion medium the female liver was found to take up more CDCA from the perfusion medium, to store more CDCA, and to convert less CDCA to β-muricholic acid. It was documented that the toxicity of CDCA for the isolated perfused liver of the female rat is not due to α- or β-muricholic acid, the end products of CDCA metabolism in the rat. The relatively greater capacity of the male liver to convert potentially toxic CDCA to nontoxic β-muricholic acid may explain, at least in part, the observed sex difference in CDCA hepatotoxicity.

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