Renal Cortical Slice Uptake and Runout of N-methylnicotinamide and p- Aminohippurate after Potassium Dichromate Treatment

1974 ◽  
Vol 52 (3) ◽  
pp. 465-468 ◽  
Author(s):  
G. H. Hirsch ◽  
A. P. Pakuts
Keyword(s):  
Adult Male ◽  
Subcutaneous Injection ◽  
Potassium Dichromate ◽  
Male Rats ◽  
Significant Enhancement ◽  
Cortical Slice ◽  
Single Subcutaneous Injection ◽  
Kidney Slices ◽  
Renal Cortical Slice ◽  
Cortical Slices

Administration of 40 mg/kg potassium dichromate to adult male rats produced a significant enhancement of N-methylnicotinamide (NMN) uptake by renal cortical slices when measured 24 h after a single subcutaneous injection. Incubation under nitrogen or in the presence of dinitrophenol reduced NMN accumulation by kidney slices from control and treated rats to the same level. The rate of NMN runout was slower in renal cortical slices from rats treated with 40 mg/kg potassium dichromate, while p-aminohippurate (PAH) runout was significantly greater than control.

ASSAY OF CORTICOTROPHIN PREDNISOLONE PRETREATMENT INSTEAD OF HYPOPHYSECTOMY IN THE ADRENAL ASCORBIC ACID DEPLETION TEST

1960 ◽  
Vol XXXV (IV) ◽  
pp. 594-603 ◽  
Author(s):  
Christian Hamburger
Keyword(s):  
Ascorbic Acid ◽  
Adult Male ◽  
Subcutaneous Injection ◽  
Aqueous Suspension ◽  
Inhibitory Effect ◽  
Male Rats ◽  
Synthetic Analogues ◽  
Single Subcutaneous Injection ◽  
Calibration Curves ◽  
Intact Rats

ABSTRACT The inhibitory effect of various corticosteroids and synthetic analogues on the stress-induced release of corticotrophin was measured in adult male rats by means of the adrenal ascorbic acid depletion test. All the compounds examined were active, dexamethasone and prednisolone being the most effective inhibitors. A single subcutaneous injection of a microcrystalline aqueous suspension of prednisolone (1.5 mg per rat), given about 2 hours before left-sided adrenalectomy, blocked completely the adrenal ascorbic acid depletion. Quantitative assays of a purified corticotrophin preparation were carried out with hypophysectomized and prednisolone-treated intact rats. The latter were less sensitive to corticotrophin, but the slopes of the calibration curves and the standard deviations appeared to be the same in both instances. It is concluded that it is feasible to replace the surgical hypophysectomy by prednisolone pretreatment in the ascorbic acid depletion test for corticotrophin.

Maintained Hypersexuality between Male Rats following Chronically Induced Limbic Seizures: Implications for Bisexuality in Complex Partial Epileptic Seizures

1994 ◽  
Vol 74 (2) ◽  
pp. 647-652 ◽  
Author(s):  
M. A. Persinger
Keyword(s):  
Adult Male ◽  
Subcutaneous Injection ◽  
Epileptic Seizures ◽  
Male Rats ◽  
Albino Rats ◽  
Limbic Seizures ◽  
Single Subcutaneous Injection

Adult male albino rats were given a treatment that produced hypothermia after the induction of limbic seizures by a single subcutaneous injection of lithium and pilocarpine. When housed in groups, these rats exhibited marked hypersexuality (for at least two months), defined as repeated mounting of another male, pelvic thrusting, and persistent genital licking; while the male was mounted, female postures were assumed. There were also periods of physical submission. During active periods three of the four rats were mounted and thrusting in tandem. Possible relevance to the Klüver-Bucy syndrome and to bisexuality and homosexuality in males who report elevated complex partial epileptic-like signs is discussed.

In-Vitro Mechanisms of 1,2-Dichloropropane Nephrotoxicity using the Renal Cortical Slice Model

1993 ◽  
Vol 12 (2) ◽  
pp. 117-121 ◽  
Author(s):  
Andrea Trevisan ◽  
Paola Meneghetti ◽  
Stefano Maso ◽  
Ornella Troso
Keyword(s):  
Organic Anion ◽  
Aminooxyacetic Acid ◽  
Cortical Slice ◽  
Major Step ◽  
Oxidative Pathway ◽  
Lyase Activity ◽  
Renal Cortical Slice ◽  
Male Wistar Rats ◽  
Cortical Slices

1 Renal cortical slices isolated from the kidneys of male Wistar rats were used as an experimental model for studying the nephrotoxicity induced by 1,2-dichloropropane. 2 The solvent causes a depletion of renal reduced glutathione content and slight, but significant, lipid peroxidation. The block of the oxidative pathway with carbon monoxide prevents glutathione content depletion, and shows that this conjugation is the major step in 1,2-dichloropropane metabolism. 3 Loss of organic anion accumulation and release into the incubation medium of tubular enzymes, mainly from the soluble fraction, are the toxic effects of the solvent. The brush border is only slightly affected. 4 The mechanism of nephrotoxicity appears to occur via mercapturic acid metabolism. Acivicin and aminooxyacetic acid, inhibitors of gammaglutamyltransferase and β-lyase activity, respectively, partially but significantly prevent the loss of organic anion accumulation induced by 1,2-dichloropropane. Furthermore, α-ketobutyrate, an activator of β-lyase, enhances the effects of 1,2-dichloropropane on the target, but is itself toxic for organic anion accumulation.

SEQUENTIAL CHANGES IN RAT THYMUS AFTER THE SUBCUTANEOUS INJECTION OF CORTISOL

1966 ◽  
Vol 35 (1) ◽  
pp. 19-27 ◽  
Author(s):  
D. BELLAMY ◽  
P. A. JANSSENS ◽  
RUTH A. LEONARD
Keyword(s):  
Weight Loss ◽  
Oxygen Uptake ◽  
Ribonucleic Acid ◽  
Subcutaneous Injection ◽  
Point Of View ◽  
Male Rats ◽  
Single Subcutaneous Injection ◽  
Sequence Of Events

SUMMARY Several properties of the thymus were examined from the point of view of the mechanism of corticosteroid-induced involution. Involution, as measured by weight loss, proceeded for 3 days after a single subcutaneous injection of cortisol (3·0 mg./100 g. body wt.) into male rats. In thymus slices there was initially a transient inhibition of oxygen uptake. Other changes, namely a decreased concentration of ribonucleic acid and an increased release of protein from incubated slices, occurred later. There was no change in the degree of penetration of inulin, sucrose or sodium into incubated slices. It is thought that the results are consistent with the concept that cortisol sets in motion a set sequence of events, and that some of the changes are not dependent on the continued presence of hormone in the gland.

Subchronic co-administration to cadmium, diazinon and selenium causes apparent symptoms of osteoporosis in adult male rats

2014 ◽  
Author(s):  
Radoslav Omelka ◽  
Hana Chovancova ◽  
Ivana Bobonova ◽  
Grzegorz Formicki ◽  
Robert Toman ◽  
...  
Keyword(s):  
Adult Male ◽  
Male Rats
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