Relative Importance of Liver and Kidney in Synthesis of Uric Acid in Chickens

1972 ◽  
Vol 50 (10) ◽  
pp. 936-939 ◽  
Author(s):  
S. T. Chou
Keyword(s):  
Uric Acid ◽  
Spectrophotometric Determination ◽  
Acid Concentration ◽  
Broiler Chickens ◽  
Organ Weight ◽  
Relative Importance ◽  
Nitrogenous Compound ◽  
Inosinic Acid ◽  
Liver And Kidney

Broiler chickens of 1, 2, and 7 months of age were used for enzymatic spectrophotometric determination of uric acid and its precursors, xanthine, hypoxanthine, and inosinic acid, in the liver and kidney in an attempt to show the relative importance of the two organs in the synthesis of uric acid, the major nitrogenous compound excreted by birds.Uric acid concentration of the kidney was two to three times as high as the liver, probably attributable in part to preformed uric acid trapped in the urinary passages in the kidney. Significantly higher concentrations of xanthine, hypoxanthine, and inosinic acid were found in the kidney than in the liver. The calculations based on organ weight and the concentrations of uric acid precursors suggest that the kidney synthesizes 96, 60, and 73% as much uric acid as does the liver for the 1-, 2-, and 7-month-old chickens, respectively.

scholarly journals LC-MS/MS analysis of doxycycline residues in chicken tissues after oral administration

2014 ◽  
Vol 58 (4) ◽  
pp. 573-579 ◽  
Author(s):  
Anna Gajda ◽  
Andrzej Posyniak ◽  
Grzegorz Tomczyk
Keyword(s):  
Oral Administration ◽  
Broiler Chickens ◽  
Thigh Muscle ◽  
Limit Of Quantification ◽  
Limit Values ◽  
Chicken Tissues ◽  
Short Period ◽  
Liquid Chromatography Tandem Mass ◽  
Liver And Kidney

Abstract For the purpose of quantitative determination of doxycycline (DC) residues in tissues, a sensitive liquid chromatography - tandem mass spectrometry (LC-MS/MS) method was developed. The method was used to determine DC residues in chicken tissues (breast and thigh muscle, liver and kidney) after oral administration with drinking water to five-weak-old broiler chickens. The DC was administered for five consecutive days at a therapeutic dose of 10 mg/kg b.w. once a day. The tissues were collected after 6 h, 24 h, 7 d, and 8 d. The method was validated and the decision limit was established for muscle - 109.2 μg/kg, for liver - 326.1 μg/kg, and for kidney - 634.0 μg/kg. The detection limit was 2 μg/kg and the limit of quantification was 5 μg/kg. In a short period after ceasing the treatment, the detected concentrations of DC were much higher than the established maximum residue limit values. The highest residue concentrations of DC were observed in the kidney, followed by the liver and muscle. The lowest concentration of DC was determined in tight muscle.

Effect of urine storage on urinary uric acid concentrations

2000 ◽  
Vol 37 (3) ◽  
pp. 355-359 ◽  
Author(s):  
Jun-Ichi Yamakita ◽  
Tetsuya Yamamoto ◽  
Yuji Moriwaki ◽  
Sumio Takahashi ◽  
Zenta Tsutsumi ◽  
...  
Keyword(s):  
Uric Acid ◽  
Acid Concentration ◽  
Storage Temperature ◽  
Accurate Determination ◽  
Uric Acid Concentration ◽  
Serum Samples ◽  
Diagnosis And Classification ◽  
Urine Storage

Accurate determination of serum and urinary uric acid concentrations is essential for the diagnosis and classification of gout according to uric acid metabolism derangement. Urine and/or serum samples are often kept at either 4°C or 20°C until assayed, when a large number of samples are handled simultaneously. Our preliminary study indicated a significant decrease in urinary uric acid concentration after preservation, regardless of the storage temperature. Uric acid crystals were often observed in these cases which showed a marked decrease in urinary uric acid concentration after storage. In the present study, we sought the factor(s) that might cause this decrease in urinary uric acid concentration, as well as measures to overcome the problem. High urinary uric acid concentration and low pH proved to play major roles in the decrease in urinary uric acid concentration after storage. In contrast, dilution of the urine samples before storage resulted in no significant change in urinary uric acid concentration. Based on these results, we recommend diluting urine before storage for determination of uric acid concentration and avoiding underestimation.

scholarly journals Spectrophotometric Determination of Uric Acid Based on Fading of o-Hydroxyhydroquinonephthalein-palladium(II)-hexadecyltrimethyl-ammonium Complex

2007 ◽  
Vol 23 (2) ◽  
pp. 223-226 ◽  
Author(s):  
Takako YAMAGUCHI ◽  
Kohei HASEGAWA ◽  
Shinichiro KAMINO ◽  
Kanako MIYACHI ◽  
Hiroshi TOMINAGA ◽  
...  
Keyword(s):  
Uric Acid ◽  
Spectrophotometric Determination
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