Report of the Medical Research Council Committee on Human Pituitary Hormones: Radioimmunoassay Kits for Human Follicle Stimulating Hormone and Luteinizing Hormone

1971 ◽  
Vol 49 (7) ◽  
pp. 685-687 ◽  
Author(s):  
C. Faiman ◽  
B. Shome
Keyword(s):  
Luteinizing Hormone ◽  
Medical Research ◽  
Research Council ◽  
Medical Research Council ◽  
Follicle Stimulating Hormone ◽  
Pituitary Hormones ◽  
Serum Samples ◽  
Human Pituitary ◽  
Stimulating Hormone

The methodology used in the development of radioimmunoassay kits for human follicle stimulating hormone (FSH) and luteinizing hormone (LH) which are ready for distribution to qualified investigators in Canada is described. Values obtained in measuring the FSH and LH content of serum samples are virtually identical to those obtained with parallel determinations using previously described radioimmunoassay techniques.

DEXTRAN-COATED CHARCOAL USED IN THE RADIOIMMUNOASSAY OF HUMAN PITUITARY LUTEINIZING HORMONE

1973 ◽  
Vol 73 (3) ◽  
pp. 444-454 ◽  
Author(s):  
T. Sand ◽  
P. A. Torjesen
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Protein Composition ◽  
Thyroid Stimulating Hormone ◽  
Human Chorionic Gonadotrophin ◽  
Human Thyroid ◽  
Serum Samples ◽  
Human Pituitary ◽  
The Difference ◽  
Stimulating Hormone

ABSTRACT A radioimmunoassay for human pituitary luteinizing hormone (LH) using charcoal for the separation of free from antibody-bound hormone is described. The ability of the various types of charcoal preparations tested to separate free from antibody-bound hormone differed greatly as did the amount required to give maximum adsorption of free hormone. It was also found that the adsorption of free and antibody-bound hormone was greatly influenced by the presence of other proteins. Hence it was necessary to add human serum to the standard tubes before the addition of the charcoal-dextran suspension, in order to compensate for the difference in protein composition between the standards and the serum samples. Two antisera obtained from rabbits immunized with human chorionic gonadotrophin (HCG) were used. One of the antisera had an affinity to human thyroid-stimulating hormone (TSH) and human follicle-stimulating hormone (FSH) of less than 10% as compared to that of LH, while the other had an affinity of about 30 % as compared to that of LH.

PURIFICATION OF CHICKEN PITUITARY FOLLICLE-STIMULATING HORMONE AND LUTEINIZING HORMONE

1969 ◽  
Vol 43 (4) ◽  
pp. 609-616 ◽  
Author(s):  
ANNE STOCKELL HARTREE ◽  
F. J. CUNNINGHAM
Keyword(s):  
Luteinizing Hormone ◽  
Follicle Stimulating Hormone ◽  
Deae Cellulose ◽  
Weight Basis ◽  
Cross Reaction ◽  
Partial Purification ◽  
Human Pituitary ◽  
Immunological Cross Reaction ◽  
Stimulating Hormone

SUMMARY Separation and partial purification of chicken pituitary follicle-stimulating hormone (FSH) and luteinizing hormone (LH) has been obtained by methods which were effective in the purification of the corresponding human and horse hormones. Increases in chicken LH activity were observed after chromatography on DEAE-cellulose and on Amberlite IRC-50 suggesting removal of an LH inhibitor. The biological potencies of chicken FSH and LH preparations when assayed in mammals were very much lower than those of the corresponding mammalian fractions on a weight basis. A weak immunological cross-reaction between chicken and human pituitary LH was used to estimate LH in chicken pituitary fractions and the results were compared with bioassays of the same fractions.

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