The metabolism of choline by a sympathetic ganglion

1969 ◽  
Vol 47 (2) ◽  
pp. 119-126 ◽  
Author(s):  
B. Collier ◽  
Celia Lang
Keyword(s):  
Sympathetic Ganglion ◽  
Superior Cervical Ganglia ◽  
Physiological Concentration ◽  
Selective Precipitation ◽  
Physiological Conditions ◽  
Phospholipid Turnover ◽  
Cervical Ganglia

Cat's superior cervical ganglia were perfused with Locke's solution containing choline (Ch) at physiological concentration but labelled (methyl-3H), and the radioactive products in extracts of such ganglia were identified by a combination of selective precipitation and chromatographic tests. Ch was incorporated into acetylcholine (ACh), into phosphorylcholine (PCh), and into phospholipid. The rate of formation of PCh and phospholipid from Ch was measured to be about 2 ng/min of each, and this rate was unaffected by activity or by hemicholinium. Free Ch liberated by PCh or phospholipid turnover is unlikely to be an important source of Ch for ACh synthesis under physiological conditions.

The source of choline for acetylcholine synthesis in a sympathetic ganglion

1969 ◽  
Vol 47 (2) ◽  
pp. 127-135 ◽  
Author(s):  
B. Collier ◽  
F. C. MacIntosh
Keyword(s):  
Sympathetic Ganglion ◽  
Perfusion Fluid ◽  
Superior Cervical Ganglia ◽  
Physiological Concentration ◽  
Acetylcholine Synthesis ◽  
Stimulation Period ◽  
Cervical Ganglia ◽  
Preganglionic Stimulation ◽  
Uptake And Release

Choline (Ch) and acetylcholine (ACh) uptake and release were measured by a combination of tracer and bioassay techniques in perfused superior cervical ganglia of the cat during rest and repetitive preganglionic stimulation. The uptake of labelled ACh as such was small; but when Ch (methyl-3H labelled) was present at physiological concentration (1.5 μg/ml) in the perfusion fluid, its incorporation into the ACh and free Ch pools of the ganglion proceeded linearly in the absence of stimulation, was accelerated by stimulation, and was inhibited by hemicholinium but not by hexamethonium. Up to 85% of ganglionic ACh could be replaced by labelled ACh during a 1-h stimulation period. On subsequent perfusion with fluid containing unlabelled Ch, labelled Ch was lost but labelled ACh was retained and could be released by stimulation, either as ACh in the presence or as Ch in the absence of eserine. The output of label during stimulation was approximately doubled by eserine but was unaffected by hemicholinium, which, however, prevented the formation and release of newly synthesized ACh. It appears that about half the Ch formed from released ACh is immediately recaptured and resynthesized into ACh. Newly synthesized ACh rapidly gains access to the releasable transmitter pool and may be preferentially released.

Relationship between golgi apparatus and axonal reticulum in sympathetic ganglion cells

1978 ◽  
Vol 36 (2) ◽  
pp. 598-599
Author(s):  
J. Quatacker ◽  
W. De Potter
Keyword(s):  
Golgi Apparatus ◽  
Sympathetic Ganglion ◽  
Phosphotungstic Acid ◽  
Ganglion Cells ◽  
Low Ph ◽  
Dense Core ◽  
Dense Core Vesicles ◽  
Large Dense Core Vesicles ◽  
Cervical Ganglia ◽  
Axoplasmic Reticulum

Mucopolysaccharides have been demonstrated biochemically in catecholamine-containing subcellular particles in different rat, cat and ox tissues. As catecholamine-containing granules seem to arise from the Golgi apparatus and some also from the axoplasmic reticulum we examined wether carbohydrate macromolecules could be detected in the small and large dense core vesicles and in structures related to them. To this purpose superior cervical ganglia and irises from rabbit and cat and coeliac ganglia and their axons from dog were subjected to the chromaffin reaction to show the distribution of catecholamine-containing granules. Some material was also embedded in glycolmethacrylate (GMA) and stained with phosphotungstic acid (PTA) at low pH for the detection of carbohydrate macromolecules.The chromaffin reaction in the perikarya reveals mainly large dense core vesicles, but in the axon hillock, the axons and the terminals, the small dense core vesicles are more prominent. In the axons the small granules are sometimes seen inside a reticular network (fig. 1).

Downregulation of P2Y12in the superior cervical ganglia alleviates abnormal sympathetic activity after myocardial ischemia

2017 ◽  
Vol 233 (4) ◽  
pp. 3375-3383 ◽  
Author(s):  
Lifang Zou ◽  
Yingxin Gong ◽  
Shanhong Zhao ◽  
Zhihua Yi ◽  
Xinyao Han ◽  
...  
Keyword(s):  
Myocardial Ischemia ◽  
Sympathetic Activity ◽  
Superior Cervical Ganglia ◽  
Cervical Ganglia

Enzyme levels in cultured astrocytes, oligodendrocytes and Schwann cells, and neurons from the cerebral cortex and superior cervical ganglia of the rat

1991 ◽  
Vol 16 (9) ◽  
pp. 991-999 ◽  
Author(s):  
Robert S. Rust ◽  
Joyce G. Carter ◽  
David Martin ◽  
Jeanne M. Nerbonne ◽  
Patricia A. Lampe ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Schwann Cells ◽  
Superior Cervical Ganglia ◽  
Cultured Astrocytes ◽  
Cervical Ganglia

Selective labelling of muscarinic M1 receptors in calf superior cervical ganglia by [3H](±)-telenzepine

1991 ◽  
Vol 195 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Roland Feifel ◽  
Jaap F.Rodrigues de Miranda ◽  
Carsten Strohmann ◽  
Reinhold Tacke ◽  
Arne J. Aasen ◽  
...  
Keyword(s):  
Superior Cervical Ganglia ◽  
Selective Labelling ◽  
M1 Receptors ◽  
Cervical Ganglia

Effect of dietary NaCl on tyrosine hydroxylase in the superior cervical ganglia of Dahl rats

1995 ◽  
Vol 190 (1) ◽  
pp. 13-16 ◽  
Author(s):  
Akihiro Nagae ◽  
Katsuhiko Kohara ◽  
Takeru Iwata ◽  
Seiji Matsuda ◽  
Masahiro Sakanaka ◽  
...  
Keyword(s):  
Tyrosine Hydroxylase ◽  
Superior Cervical Ganglia ◽  
Cervical Ganglia

GABAA Receptor Subunit mRNAs in Rat Superior Cervical Ganglia

Pharmacology ◽  
1999 ◽  
Vol 58 (1) ◽  
pp. 51-58 ◽  
Author(s):  
Zhi Fang Liu ◽  
David R. Burt
Keyword(s):  
Receptor Subunit ◽  
Superior Cervical Ganglia ◽  
Cervical Ganglia
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