Changes in DNA and RNA in the rat adenohypophysis following bilateral adrenalectomy, sham adrenalectomy, and the chronic injection of cortisol

1968 ◽  
Vol 46 (3) ◽  
pp. 431-439 ◽  
Author(s):  
Jacob Kraicer ◽  
Su Chiau Cheng
Keyword(s):  
Nucleic Acid ◽  
Feedback Control ◽  
Acid Content ◽  
Transient Increase ◽  
Bilateral Adrenalectomy ◽  
Nucleic Acid Content ◽  
Dna And Rna ◽  
Rna Content ◽  
Chronic Injection

Changes in adenohypophyseal DNA and RNA content were determined as a function of time following adrenalectomy, sham adrenalectomy, and the chronic injection of cortisol. The stess of operation resulted in a transient increase in adenohypophyseal nucleic acid content, whereas the increase in secretion of ACTH induced by feedback control did not. An attempt is made to correlate the changes in nucleic acid content with the concurrent changes in the intensity of biosynthesis of ACTH.

Influence of the RNA content on oogenesis in the bobbed mutants of Drosophila melanogaster

Development ◽  
1971 ◽  
Vol 25 (2) ◽  
pp. 237-246
Author(s):  
Jag Mohan
Keyword(s):  
Drosophila Melanogaster ◽  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Wild Type ◽  
Dna And Rna ◽  
Stage Of Development ◽  
Rna Content ◽  
Egg Chambers ◽  
Total Dna

Developing egg chambers of Drosophila melanogaster (wild-type and bobbed mutants) have been examined for their nucleic acid content by cytophotometric methods. No differences were observed in the total DNA and RNA content of the egg chambers at all stages between the bobbed mutants and the wild type. It is shown that the process of oogenesis in bobbed females is prolonged, and that this prolongation occurs at all the stages of oocyte development. Since the ovaries of the bobbed females synthesize less rRNA per unit time, it is likely that this prolongation allows the egg chambers of the bobbed females to normalize their RNA content. When they achieve a given RNA content, they proceed to the next stage of development.

CHANGES IN ADENOHYPOPHYSEAL CYTOLOGY AND NUCLEIC ACID CONTENT IN THE RAT 32 DAYS AFTER BILATERAL ADRENALECTOMY AND THE CHRONIC INJECTION OF CORTISOL

1967 ◽  
Vol 45 (6) ◽  
pp. 947-956 ◽  
Author(s):  
Jacob Kraicer ◽  
Marc Herlant ◽  
Pierre Duclos
Keyword(s):  
Cell Types ◽  
Bilateral Adrenalectomy ◽  
Synthetic Activity ◽  
Nucleic Acid Content ◽  
Cell Type ◽  
Cytological Evidence ◽  
Histological Techniques ◽  
Dna And Rna ◽  
Distinct Cell ◽  
Chronic Injection

Control, adrenalectomized, and cortisol-treated rats were maintained under rigidly controlled conditions, and the adenohypophyses were examined histologically with two staining procedures which differentiate six distinct cell types. Only one cell type demonstrated cytological evidence of increased synthetic activity 32 days after adrenalectomy (the changes were, however, minimal) and decreased synthetic activity following the chronic injection of cortisol. This cell type, which we designate as the corticotroph, would be classed as a chromophobe (no stainable granules) with use of standard histological techniques, but is, in fact, as Herlant's Tetrachrome demonstrates, a distinct acidophilic cell type different from the prolactin cell and the somatotroph. The determination of adenohypophyseal DNA and RNA revealed no evidence of increased protein synthetic activity following bilateral adrenalectomy, but did reveal evidence of decreased protein synthetic activity following the chronic injection of cortisol.

scholarly journals Ameliorating effects of Vit-C on protein and nucleic acid content in dimecron intoxicated chick embryos

2016 ◽  
Vol 2 ◽  
pp. 20-27
Author(s):  
Biplab Kumar Behera ◽  
Keyword(s):  
Nucleic Acid ◽  
Toxic Effect ◽  
Quantitative Study ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Chick Embryos ◽  
Dna And Rna ◽  
Hen’S Egg ◽  
Made In ◽  
Vit C

Dimecron when introduced into the fertilized hen’s egg at a certain dose before incubation shows a characteristic and interesting feature which has been studied and discussed. A quantitative study of proteins from different organs viz. liver, kidney and brain has been made in the present study. A significant reduction in the quantity of the protein of all the organs is observed. The study witnessed the activity of DNA and RNA that undergoes a decline in its quantity by the action of the pesticide. The toxic effect of pesticides is recovered by the use of ameliorating agent like Vit C. Keywords: Atropine, DNA, organophosphate, protein, RNA, Vit-C.

scholarly journals THE RIBONUCLEIC ACID AND DEOXYRIBONUCLEIC ACID CONTENT OF MONONUCLEAR LEUKOCYTES OF DIFFERENT SIZES STUDIED BY ULTRAVIOLET CYTOPHOTOMETRY

1973 ◽  
Vol 21 (7) ◽  
pp. 628-633 ◽  
Author(s):  
ALFREDO MARIANO GARCIA ◽  
PATRICIA A. N. SULLIVAN
Keyword(s):  
Nucleic Acid ◽  
Cell Size ◽  
Dna Content ◽  
Random Error ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Nucleic Acid Content ◽  
Mononuclear Leukocytes ◽  
Total Nucleic Acid ◽  
Dna And Rna

Rat mononuclears (lymphocytes and monocytes) were studied for total nucleic acid content by means of ultraviolet cytophotometry. Another set was treated with ribonuclease, and deoxyribonucleic acid (DNA) was measured using the same technique. It was found that total nucleic acid content (DNA and RNA) increases linearly with cell size from about 20 units in lymphocytes having 5 µ in diameter up to around 30 units in cells having 12-14 µ in diameter; this is to say, an almost 50% increase for a 6-7-fold enlargement. After ribonuclease treatment, however, the value of the integrated extinction (DNA) tends to remain constant for different cell sizes. A 650% variation in area is accompanied by a DNA change of less than 6%. The differences between treated and nontreated cells are nonsignificant for populations having up to 7.0-7.5 µ in diameter, which implies that small lymphocytes either have a negligible amount of RNA or that the instrument is not sensitive enough to detect it (less than 7% of the DNA content, this figure being the random error of our technique). These differences become highly significant for mononuclears having 8 µ or more in diameter. Therefore, while DNA tends to be constant and independent from cell size, RNA content tends to be harmoniously inconstant, since it is correlated with cell (and nuclear) size and degree of chromatin diffusion.

EFFECTS OF ADMINISTERING ANTISERA TO MOUSE GROWTH HORMONE AND PROLACTIN ON GAIN IN LITTER WEIGHT AND ON MAMMARY NUCLEIC ACID CONTENT OF LACTATING C3H MICE

1972 ◽  
Vol 55 (1) ◽  
pp. 31-NP ◽  
Author(s):  
Y. N. SINHA ◽  
U. J. LEWIS ◽  
W. P. VANDERLAAN
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content ◽  
Ovarian Weight ◽  
Litter Weight ◽  
Dna And Rna ◽  
Lactating Mothers ◽  
C3h Mice

SUMMARY The effects of administering antiserum to mouse growth hormone (GH) and prolactin on lactation and mammary nucleic acid content of C3H mice were studied. Injections of either antisera to lactating mothers suppressed the gain in body weight of their suckling young, GH antiserum more readily than prolactin antiserum. A combined injection of the two antisera resulted in an immediate retardation of the gain in body weight of the young. Although both antisera reduced DNA and RNA content of the mammary gland, GH antiserum appeared to affect DNA content predominantly whereas prolactin antiserum affected RNA: DNA ratio, suggesting involvement of GH primarily in cell proliferation and of prolactin in protein synthesis. Both antisera abolished the gain in maternal body weight. Mouse GH antiserum decreased maternal adrenal weight whereas both antisera reduced ovarian weight. The results suggest that both GH and prolactin may be involved in normal lactation and in maintenance of body weight in C3H mice.

scholarly journals Nucleic Acid Content in Male Fresh Water Fish N. notopterus Exposed to Copper Sulphate

2015 ◽  
Vol 33 ◽  
pp. 1-8
Author(s):  
K. Ravikiran ◽  
R.S. Kulkarni
Keyword(s):  
Nucleic Acid ◽  
Freshwater Fish ◽  
Acid Content ◽  
Copper Sulphate ◽  
Nucleic Acid Content ◽  
Fresh Water Fish ◽  
Exposed Fish ◽  
Rna Content ◽  
Water Fish ◽  
Made In

The nucleic acid content in different tissues such as brain, liver, kidney & testis of both control and copper sulphate (CuSO4) exposed freshwater fish N. notopterus has been studied. The following observation is made in both control and copper sulphate (CuSO4) exposed fish. The testis contain large amount of DNA in comparison to other tissues. The degree of DNA content in control and copper sulphate (CuSO4) exposed fish testis >liver>brain>kidney. The RNA content also exhibited similar to that of DNA, having higher amount in the testis. The degree of RNA content in control and copper sulphate (Cuso4) exposed fish testis >liver>brain>kidney. The nucleic acid content of tissues get reduced under copper sulphate (CuSO4) exposed in the male freshwater fish N. notopterus indicating copper sulphate as a pollutant effect the nucleic acid content in the tissue.

Application of Ogur & Rosen's Method for the Determination of Nucleic Acid Content of Bacteria

1957 ◽  
Vol 12 (2) ◽  
pp. 125-129 ◽  
Author(s):  
Nobuyasu KAWASAKI ◽  
Ichiro TAKI ◽  
Chiaki WATANABE ◽  
Kiyoshi MATOBA ◽  
Mokichiro NISHIO ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Acid Content ◽  
Nucleic Acid Content

scholarly journals Using flow cytometry and light-induced fluorescence technique to characterizethe variability and characteristics of bioaerosols in springtime at Metro Atlanta, Georgia

2018 ◽  
Author(s):  
Arnaldo Negron ◽  
Natasha DeLeon-Rodriguez ◽  
Samantha M. Waters ◽  
Luke D. Ziemba ◽  
Bruce Anderson ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Nucleic Acid ◽  
Acid Content ◽  
Fungal Spores ◽  
Epifluorescence Microscopy ◽  
Nucleic Acid Content ◽  
Rain Event ◽  
Bacterial Cells ◽  
Sampling System ◽  
Spores And Pollen

Abstract. The abundance and speciation of primary biological aerosol particles (PBAP) is important for understanding their impacts on human health, cloud formation and ecosystems. Towards this, we have developed a protocol for quantifying PBAP collected from large volumes of air with a portable wet-walled cyclone bioaerosol sampler. A flow cytometry (FCM) protocol was then developed to quantify and characterize the PBAP populations from the sampler, which were confirmed against epifluorescence microscopy. The sampling system and FCM analysis were used to study PBAP in Atlanta, GA over a two-month period and showed clearly defined populations of DNA-containing particles: Low Nucleic Acid-content particles (bioLNA), High Nucleic Acid-content particles (HNA) being fungal spores and pollen. We find that daily-average springtime PBAP concentration (1 to 5 μm diameter) ranged between 1.4 × 104 and 1.1 × 105 m−3. The BioLNA population dominated PBAP during dry days (72 ± 18 %); HNA dominated the PBAP during humid days and following rain events, where HNA (e.g., wet-ejected fungal spores) comprised up to 92 % of the PBAP number. Concurrent measurements with a Wideband Integrated Bioaerosol Sensor (WIBS-4A) showed that FBAP and total FCM counts are similar; HNA (from FCM) significantly correlated with ABC type FBAP concentrations throughout the sampling period (and for the same particle size range, 1–5 μm diameter). However, the FCM bioLNA population, possibly containing bacterial cells, did not correlate to any FBAP type. The lack of correlation of any WIBS FBAP type with the bioLNA suggest bacterial cells may be more difficult to detect with autofluorescence than previously thought. Ιdentification of bacterial cells even in the FCM (bioLNA population) is challenging, given that the fluorescence level of stained cells at times may be comparable to that seen from abiotic particles. HNA and ABC displayed highest concentration on a humid and warm day after a rain event (4/14), suggesting that both populations correspond to wet-ejected fungal spores. Overall, information from both instruments combined reveals a highly dynamic airborne bioaerosol community over Atlanta, with a considerable presence of fungal spores during humid days, and a bioLNA population dominating bioaerosol community during dry days.

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