EFFECT OF COLD EXPOSURE ON SERUM LIPIDS AND LIPOPROTEINS IN THE RAT

1966 ◽  
Vol 44 (5) ◽  
pp. 711-719 ◽  
Author(s):  
M. W. Radomski
Keyword(s):  
Cold Exposure ◽  
Serum Lipids ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Total Serum ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Triglyceride Fraction ◽  
Lipids And Lipoproteins

The effect of cold exposure for 1–52 days on the concentration and composition of the serum lipids and lipoproteins in male Wistar rats was studied. Serum lipoproteins were separated by preparative ultracentrifugation into three density classes, very low density (d < 1.006), low-density (d, 1.006–1.070), and high-density (d, 1.070–1.218). On the first day of cold exposure, total serum lipids were reduced by 20–25%, this decrease occurring mainly in the triglyceride fraction. Although the low-density and high-density lipoproteins were not affected by cold exposure, the concentration of the very low density lipoproteins fell markedly to 70–75% below normal levels. These alterations in the pattern of serum lipids and lipoproteins persisted throughout the cold-exposure period. Sex or prior feeding of a high-fat, high-carbohydrate, or high-protein diet did not modify these cold-induced changes. The response of the very low density lipoproteins to cold was immediate and rapid, the rate and degree of change being greatest in the first 4 hours of exposure. Conversely, the recovery of the very low density fraction to normal after removal of the rats from the cold was slow and depended upon the period of prior exposure. Animals exposed to 4 °C for 4 days required 3 days at 22 °C for the very low density lipoproteins to return to normal, and the recovery period was twice as long for cold-acclimatized (4 weeks) animals. The role of the triglyceride-rich very low density lipoproteins as a readily available energy source in the animals is discussed in the light of these results.

Effects of Carbohydrate Feeding on Serum Lipids and Lipoproteins in the Rat

1957 ◽  
Vol 189 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Joseph H. Bragdon ◽  
Richard J. Havel ◽  
Robert S. Gordon
Keyword(s):  
Serum Lipids ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Lipid Transport ◽  
Cholesterol Concentration ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Serum Cholesterol Concentration ◽  
Carbohydrate Feeding ◽  
Lipids And Lipoproteins

Fasting in the rat is accompanied by an increase in serum cholesterol concentration reflecting an increase in high-density lipoproteins. The feeding of carbohydrate results in decreases in both low- and high-density lipoproteins, the former occurring acutely, and the latter occurring more slowly. In the fasting rat the injection of protamine, an antiheparin agent, produces an increase in all serum lipids, but the increase occurs in the low-density lipoproteins. In the rat fed carbohydrate this lipemia-inducing effect of protamine is practically abolished. The feeding of carbohydrate has no effect, however, on the rate of clearance of intravenously administered chylomicrons. These phenomena are discussed in relation to current theories of lipid transport.

scholarly journals Characterization of plasma lipoproteins separated and purified by agarose-column chromatography

1974 ◽  
Vol 139 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Lawrence L. Rudel ◽  
Jason A. Lee ◽  
Manford D. Morris ◽  
James M. Felts
Keyword(s):  
Human Plasma ◽  
Column Chromatography ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoproteins ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Simple Method ◽  
Lipoprotein Class

1. A simple method for isolation of individual human plasma lipoprotein classes is presented. In this technique, lipoproteins are removed from plasma at d1.225 by ultracentrifugation, after which they are separated and purified by agarose-column chromatography. 2. Three major classes are obtained after agarose-column chromatography. Separation between classes is excellent; more than 95% of the lipoproteins eluted from the column are recovered in the form of a purified lipoprotein class. 3. Each lipoprotein class was characterized immunologically, chemically, electrophoretically and by electron microscopy. A comparison of the properties of the column-isolated lipoproteins was made with very-low-density lipoproteins, low-density lipoproteins, and high-density lipoproteins separated by sequential ultracentrifugation at densities of 1.006, 1.063 and 1.21 respectively. 4. By each criterion, peak-I lipoproteins from the agarose column are the same as very-low-density lipoproteins, peak-II lipoproteins are the same as low-density lipoproteins, and peak-III lipoproteins are the same as high-density lipoproteins. Thus the lipoprotein classes isolated by both methods are similar if not identical. 5. The agarose-column separation technique offers the advantage of a two- to three-fold saving in time. In addition, the column-elution pattern serves as a recording of the size distribution of lipoproteins in plasma. 6. The most complete characterization is reported for human plasma lipoproteins. The results with rhesus-monkey and rabbit lipoproteins were identical.

Determination of high-density lipoproteins: screening methods compared.

1979 ◽  
Vol 25 (6) ◽  
pp. 939-942 ◽  
Author(s):  
G M Kostner ◽  
P Avogaro ◽  
G B Bon ◽  
G Cazzolato ◽  
G B Quinci
Keyword(s):  
Dextran Sulfate ◽  
Screening Program ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Total Serum ◽  
Screening Methods ◽  
Low Density ◽  
Apolipoprotein A ◽  
Immunochemical Quantitation

Abstract Factors reflecting the concentration of high-density lipoproteins in serum were assessed for 108 men and 106 women participating in a Venetian screening program for hyperlipoproteinemia. The methods applied, optimized in our laboratory, were: (a) cholesterol in high-density lipoproteins, determined in the supernate after sedimentation of the very-low-density lipoproteins + low-density lipoproteins with dextran sulfate or sodium phosphotungstate; and (b) immunochemical quantitation of apolipoprotein A-I and apolipoprotein A-II by Laurell's "rocket" technique. The latter determinations were performed with total serum before and after delipidation with diispropyl ether/n-butanol (6/4 by vol). The dextran sulfate method gave about 5% higher values than did the phosphotungstate method, but the correlation between the two was excellent (r = 0.95). Results of the immunochemical quantitation indicate that delipidation of lipoproteins before Laurell electrophoresis may not be necessary if only freshly drawn sera are used.

Comparison of two micromethods for determination of lipoprotein cholesterol in plasma.

1979 ◽  
Vol 25 (10) ◽  
pp. 1795-1798 ◽  
Author(s):  
I R Kupke ◽  
S Zeugner ◽  
A Gottschalk
Keyword(s):  
Population Sample ◽  
Low Density Lipoproteins ◽  
High Density ◽  
Plasma Lipoprotein ◽  
Lipoprotein Cholesterol ◽  
High Density Lipoproteins ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Long Distance

Abstract We compared the results obtained by a micromethod for the determination of plasma lipoprotein cholesterol, in which electrophoresis is used to separate the lipoprotein fractions (beta-, pre-beta-, and alpha-lipoproteins), with those determinations with ultracentrifugation (low-density, very-low-density, and high-density lipoproteins). Precision of determination (coefficient of variation, CV, %) was the same for beta- and low-density lipoproteins (1.6%), and for pre-beta- and very-low-density lipoproteins (3.7%); however, determination of alpha-lipoprotein cholesterol was more precise (1.4%) than that of high-density lipoprotein cholesterol (3.1%). Analytical recovery of lipoprotein cholesterol was the same for both methods (98--100%) and the results were closely correlated (r = 0.943). The procedure has been used to determine the cholesterol content of plasma lipoprotein fractions of apparently healthy adults (both sexes). Lipoprotein cholesterol concentrations in our population sample compare well with those reported for other groups of similar age, in particular Stanford long-distance runners.

The therapeutic effect of some drugs and vegetable juices on the lipid profiles in male rabbits induced atherosclerosis.

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Sawsan Taha Ahmed al-Haddad ◽  
Zaid Mohammed Mubarak Almahdawi ◽  
Munife S. Ahmed Al-janabi
Keyword(s):  
Green Tea ◽  
Density Lipoprotein ◽  
Lemon Juice ◽  
Low Density Lipoproteins ◽  
High Density ◽  
High Density Lipoproteins ◽  
Control Group ◽  
Low Density ◽  
Very Low Density Lipoproteins ◽  
Group 5

This study was designed to test the therapeutic efficacy of some hypotensive drugs and vegetable drinks on some biochemical indicators in male rabbits, where atherosclerosis was developed using 1% cholesterol with food. This study was conducted in June until the end of July 2017 in the Pharmacology Department/ General Company for Pharmaceutical Industry in Samarra. In the study, 50 local rabbits were randomly distributed by 10 groups each containing 5 animals. The first group considered as the control group. The second group is the control group treated with 1% cholesterol with the food, the third group treated with cholesterol (1% and captopril 0.71 mg), group 4 (cholesterol 1% with atenolol 0.71 mg / kg), group 5 (cholesterol 1%, amlodipine 0.07 mg / kg) , group 6 treated with cholesterol 1% and aldomet (0.57 mg / kg), group 7 (cholesterol 1% and furosemide at 3.5 mg / kg), group 8 (cholesterol 1% with garlic syrup 2 ml), group 9 treatment cholesterol 1% and lemon juice), and group 10 Treatment with (1% cholesterol and green tea syrup 2 ml). The results of the study showed a significant increase (P≤0.01)) at the level of each of cholesterol triple and triglycerides, proteins and low density lipoproteins, very low density lipoproteins, also led to obtain a significant decrease in the level of high-density lipoproteins (HDL) in the treatment group with cholesterol 1% compared to control group. At the time of the treatment of anti- pressure drugs: Captopril, Atenolol, Amlodipine ,Aldomet, and Furosmide , there were no significant differences in the cholesterol level of all pharmacological groups. Moral differences were not found in LDL-C and there was a significant decrease (P≤0.01) of the level of triglycerides, proteins and very low density lipoproteins, and there was a significant increase in the level of high-density lipoproteins HDL-C, while treatment with plant juices, there was a significant decrease (P≤0.01) in the level of total cholesterol and triglycerides and LDL, and VLDL, high-density lipoprotein (HDL-C) increased when treated with garlic, lemon and green tea. We conclude pressure drugs of any kind can cure atherosclerosis or prevent high fat, unlike its counterparts OF plants, which have shown a significant effect on controlling lipid profile and reducing their effects and future risks on the heart.

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