EXTRACTION AND CHEMICAL CHARACTERISTICS OF ANOREXIGENIC AND FAT-MOBILIZING SUBSTANCES FROM RAT URINE

1964 ◽  
Vol 42 (5) ◽  
pp. 647-655 ◽  
Author(s):  
John R. Beaton ◽  
A. J. Szlavko ◽  
J. A. F. Stevenson
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Glutamic Acid ◽  
Thin Layer Chromatography ◽  
Alkaline Solution ◽  
Chemical Characteristics ◽  
Rat Urine ◽  
Layer Chromatography

An anorexigenic and fat-mobilizing substance (FMS I), extracted from the urine of fasting rats, has been further fractionated into two materials, FMS IA and FMS IB, soluble in alkaline solution or water respectively. These two fractions have been shown to be chemically distinct by thin layer chromatography, electrophoresis, and analyses for nitrogen, carbohydrate, hexosamine, phosphorus, and "cholesterol". Further, the lipolytic activities of these extracts in vitro differ and are in the order FMS IB > FMS I > FMS IA. It has been tentatively concluded that FMS I and IA contain the 17 amino acids tryptophane, phenylalanine, leucine, arginine, isoleucine, tyrosine, valine, alanine, proline, serine, histidine, glycine, aspartic acid, glutamic acid, cystine, threonine, and lysine. FMS IB appears to contain the same amino acids with the exception of valine (which is absent). These are complex substances, the precise nature of which remains to be elucidated. It appears that the anorexigenic property of FMS I is attributable to the IA component, and the fat-mobilizing property in vivo to the IB component.

Phytochemical, Analytical and Medicinal Studies of Holoptelea integrifolia Roxb. Planch - A Review

2019 ◽  
Vol 5 (4) ◽  
pp. 270-277 ◽  
Author(s):  
Vijay Kumar ◽  
Simranjeet Singh ◽  
Ragini Bhadouria ◽  
Ravindra Singh ◽  
Om Prakash
Keyword(s):  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Biologically Active ◽  
Toxicological Studies ◽  
Wide Range ◽  
Layer Chromatography

Holoptelea integrifolia Roxb. Planch (HI) has been used to treat various ailments including obesity, osteoarthritis, arthritis, inflammation, anemia, diabetes etc. To review the major phytochemicals and medicinal properties of HI, exhaustive bibliographic research was designed by means of various scientific search engines and databases. Only 12 phytochemicals have been reported including biologically active compounds like betulin, betulinic acid, epifriedlin, octacosanol, Friedlin, Holoptelin-A and Holoptelin-B. Analytical methods including the Thin Layer Chromatography (TLC), High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Liquid Chromatography With Mass Spectral (LC-MS) analysis have been used to analyze the HI. From medicinal potency point of view, these phytochemicals have a wide range of pharmacological activities such as antioxidant, antibacterial, anti-inflammatory, and anti-tumor. In the current review, it has been noticed that the mechanism of action of HI with biomolecules has not been fully explored. Pharmacology and toxicological studies are very few. This seems a huge literature gap to be fulfilled through the detailed in-vivo and in-vitro studies.

SULFATIDES AND GLYCOLIPIDS IN PLATELETS AND ENDOTHELIAL CELLS

1987 ◽  
Author(s):  
K P Schick ◽  
S Shapiro ◽  
G Tuszynski ◽  
J Slawek
Keyword(s):  
Endothelial Cells ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Specific Binding ◽  
Blood Platelets ◽  
Primary Cultures ◽  
Galactosyl Ceramide ◽  
Layer Chromatography

Sulfatides are sulfated glycolipids which are negatively charged and thought to influence receptor mediated activities. Sulfatides have the capacity to provide a surface for the initiation of in vitro coagulation tests and these acidic lipids represent the potential biological surface for the initiation of the contact and intrinsic systems in vivo. Several sulfatides have been demonstrated in blood platelets. We have investigated sulfatides and other glycolipids in endothelial cells and platelets in order to define the cellular sources for sulfatides that would be available for influencing hemostasis. Endothelial cells were derived from primary cultures of human umbilical veins and human platelets were obtained from freshly-collected blood. Cellular lipids were extracted by the Folch method. Sulfatides and glycolipids were purified by silicic acid chromatography, separated by thin-layer chromatography, and quantitated by the assay of sphingosine. Glycolipids were also analyzed by HPLC. Globoside was found to be the predominant glycolipid in endothelial cells while lactosyl ceramide was the predominant glyco-lipid in platelets. Sulfatides were detected by two approaches: 1) Sulfatide synthesis by the incorporation of [35S]-Sulfate; 2) The specific binding of [125I]-thrombospondin and [125I]-von Willebrand’s factor (vWF) to sulfatides separated by thin-layer chromatography (TLC). Several sulfatides were identified in endothelial cells and platelets by virtue of the incorporation of [35S]-sulfate into glycolipids separated by TLC. [125I]-TSP and [125I]-vWF bound to the glycolipids that had incorporated [35S]-sulfate. [35S]-sulfate was primarily incorporated into sulfated galactosyl ceramide but both cells also synthesized complex glycolipids. TSP and vWF were shown to bind to sulfated galactosyl ceramide, a band that comigrated with glycosyl ceramide as well as with two more complex sulfatides in both cells. However, differences in sulfatide synthesis and binding of TSP to sulfatides were observed in endothelial cells from that in platelets. The study indicates that endothelial cells and platelets contain several sulfatides and thus are potential sources for sulfatides for the initiation of coagulation.

THIN-LAYER CHROMATOGRAPHY OF AMINO ACIDS: A REVIEW

2012 ◽  
Vol 35 (11) ◽  
pp. 1497-1516 ◽  
Author(s):  
S. A. Bhawani ◽  
M. N. Mohamad Ibrahim ◽  
O. Sulaiman ◽  
R. Hashim ◽  
A. Mohammad ◽  
...  
Keyword(s):  
Amino Acids ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Layer Chromatography
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