A COMPARISON OF METHODS FOR THE ISOLATION OF DEOXYRIBONUCLEIC ACID FROM SMALL AMOUNTS OF RAT TISSUE

1962 ◽  
Vol 40 (1) ◽  
pp. 1167-1177
Author(s):  
C. Mezei ◽  
S. H. Zbarsky
Keyword(s):  
Amino Acids ◽  
Intestinal Mucosa ◽  
Deoxyribonucleic Acid ◽  
Phosphorus Content ◽  
Comparison Of Methods ◽  
Nitrogen And Phosphorus ◽  
Extinction Coefficients ◽  
Rat Tissue ◽  
Improved Technique ◽  
Purines And Pyrimidines

Different procedures were studied for the isolation of deoxyribonucleic acid from liver and intestinal mucosa of the rat. The DNA preparations were compared with respect to intrinsic viscosities, extinction coefficients, nitrogen and phosphorus content, and base ratios. Hydrolyzates of the DNA samples contained amino acids indicating the presence of protein or peptides in the preparations. An improved technique was developed for the elution of purines and pyrimidines from paper chromatograms.

A COMPARISON OF METHODS FOR THE ISOLATION OF DEOXYRIBONUCLEIC ACID FROM SMALL AMOUNTS OF RAT TISSUE

1962 ◽  
Vol 40 (9) ◽  
pp. 1167-1177 ◽  
Author(s):  
C. Mezei ◽  
S. H. Zbarsky
Keyword(s):  
Amino Acids ◽  
Intestinal Mucosa ◽  
Deoxyribonucleic Acid ◽  
Phosphorus Content ◽  
Comparison Of Methods ◽  
Nitrogen And Phosphorus ◽  
Extinction Coefficients ◽  
Rat Tissue ◽  
Improved Technique ◽  
Purines And Pyrimidines

Different procedures were studied for the isolation of deoxyribonucleic acid from liver and intestinal mucosa of the rat. The DNA preparations were compared with respect to intrinsic viscosities, extinction coefficients, nitrogen and phosphorus content, and base ratios. Hydrolyzates of the DNA samples contained amino acids indicating the presence of protein or peptides in the preparations. An improved technique was developed for the elution of purines and pyrimidines from paper chromatograms.

scholarly journals The presence of 5-hydroxymethylcytosine in animal deoxyribonucleic acid

1972 ◽  
Vol 126 (4) ◽  
pp. 781-790 ◽  
Author(s):  
N. W. Penn ◽  
R. Suwalski ◽  
C. O'Riley ◽  
K. Bojanowski ◽  
R. Yura
Keyword(s):  
Deoxyribonucleic Acid ◽  
Phosphorus Content ◽  
Alkaline Solutions ◽  
Small Scale ◽  
Rat Tissues ◽  
Adult Rat ◽  
Frog Brain ◽  
Rat Brain And Liver ◽  
Scale Preparation ◽  
Rat Tissue

A method is given for small-scale preparation of DNA from 1.0–1.5g of adult rat tissues. The product from brain or liver is characterized by base ratios and phosphorus content which accord with reported values for rat tissue. It is reasonably free of RNA, protein and glycogen. It contains 5-hydroxymethylcytosine at a content of about 15% of the total cytosine bases present. 5-Hydroxymethylcytosine is also demonstrable in mouse and frog brain DNA and in the crude cytidylic acid fractions obtained from RNA hydrolysates of rat brain and liver. 5-Hydroxymethylcytosine is identified by paper chromatography, u.v. spectra in acid and alkaline solutions and by its conversion into 5-hydroxymethyluracil.

Assessment of bioavailable nitrogen and phosphorus content in the sediments of Indian mangroves

2021 ◽  
Author(s):  
Yudhistir Reddy ◽  
Dipnarayan Ganguly ◽  
Gurmeet Singh ◽  
Mamidala Harikrishna Prasad ◽  
Paneer Selvam Arumughan ◽  
...  
Keyword(s):  
Phosphorus Content ◽  
Nitrogen And Phosphorus ◽  
Indian Mangroves

scholarly journals Investigation of the decomposition and leaching dynamics of Salix, Populus and mixed leaves in the area of Lake Balaton and Kis-Balaton Wetland

2019 ◽  
pp. 119-124
Author(s):  
Brigitta Simon ◽  
Tamás Kucserka ◽  
Angéla Anda
Keyword(s):  
Total Nitrogen ◽  
Phosphorus Content ◽  
Mesh Size ◽  
Common Species ◽  
Coastal Vegetation ◽  
Lake Balaton ◽  
Decomposition Rates ◽  
Nitrogen And Phosphorus ◽  
Major Nutrient ◽  
Nitrogen Contents

In lakes and wetlands, leaf litter input from the coastal vegetation represents a major nutrient load and plays a basic structural and functional role in several ecosystems. In Hungary, at the banks of lakes and wetlands, Salix and Populus trees are the most common species. In an experiment in Lake Balaton and Kis-Balaton Wetland between 16 November 2017 to and 3 June 2018, the decomposition rates and leaching dynamics of Salix, Populus and mixed leaves (50% Salix and 50% Populus) were investigated. Total nitrogen and phosphorus content of biomass samples were measured at the beginning and end of the experiment for the leaching dynamics experiment. We found that litter mass losses (Salix, Populus and mixed leaves) were not significantly different between the two mesh size litterbags and between Lake Balaton and Kis-Balaton Wetland. Different amounts of the total nitrogen and phosphorus leaching from Salix, Populus and mixed leaves were detected. The total nitrogen contents of the plant samples were around 8-18% at the end of the investigated period. Slightly higher values were measured compared to phosphorous (27-29%).

scholarly journals Effect of Intercropping and Fertility Levels on the Nitrogen, Phosphorus and Potash Content and Uptake by Summer Pearlmillet (Pennisetum glaucum L.) under South Gujarat Condition

2021 ◽  
Vol 9 (3) ◽  
pp. 174-179
Author(s):  
K. R. Patel ◽  
Keyword(s):  
Pearl Millet ◽  
Phosphorus Content ◽  
Pennisetum Glaucum ◽  
P Uptake ◽  
Green Gram ◽  
Treatment Combination ◽  
K Uptake ◽  
Nitrogen And Phosphorus ◽  
College Of Agriculture ◽  
Nitrogen Phosphorus

A field experiment was conducted at College Agronomy Farm, N. M. College of Agriculture, Navsari Agricultural University, Navsari during summer, 2019 and 2020 comprising four intercropping treatments i.e. pearlmillet sole, pearlmillet+greengram, pearlmillet+cowpea, pearlmillet+clusterbean and three fertility levels viz., 75 % RDF, 100 % RDF and 125 % RDF. Among the different intercropping system examined, sole pearl millet (I1) accumulated significantly less content of N and P in pearl millet grain and straw as compared to intercropping with pulses treatments. Pearl millet + green gram (I2) recorded significantly higher N and P uptake by pearl millet straw than other intercropping systems. The sole pearl millet (I1) was at par with pearl millet intercropped with green gram (I2) had significantly higher K content and uptake in pearl millet grain and straw. In case of fertility levels, nitrogen and phosphorus content and uptake in pearl millet grain and straw were recorded significantly higher by application of 100 % RDF. Significantly higher N, P and K uptake by pearl millet grain and straw in pooled results were produced by treatment combination of pearl millet + green gram (I2) intercropping along with 75 % RDF (F1).

scholarly journals Quality Assessment of Some Freshwater Resources Located in Bucharest and Surrounding Areas. III. Case Study: Trophic Status Assessment of Mogosoaia, Herastrau and Pantelimon Lakes

2019 ◽  
Vol 70 (11) ◽  
pp. 3778-3782
Author(s):  
Violeta Monica Radu ◽  
Petra Ionescu ◽  
Gyorgy Deak ◽  
Alexandru Anton Ivanov ◽  
Elena Diacu ◽  
...  
Keyword(s):  
Quality Assessment ◽  
Trophic Status ◽  
Phosphorus Content ◽  
Anthropogenic Activities ◽  
Quality Parameters ◽  
Nitrogen And Phosphorus ◽  
High Accumulation ◽  
Freshwater Resources ◽  
Depth Analysis ◽  
Surrounding Areas

The aim of this paper is the evaluation of the trophic status of three lakes riparian to the Colentina River, namely Mogosoaia, Herastrau, Pantelimon, as Part III of an extended study on evaluation of the freshwater resources quality in Bucharest and surrounding areas [1, 2], supplementing the general quality assessment based on indices and indexes presented in Part I with a more in depth analysis of nutrient speciation and partitioning between water and upper sedimentary layers. Water and sediment samples were taken from the inlet and outlet areas of each lake in two sampling campaigns in July 2016 and September 2016. Some of the nutrient related water quality parameters presented in Part I of this series have been used here, together with the analysis of total nitrogen and total phosphorus content in sediments, to assess the general trophic state of the lakes and to highlight the relationships between the nitrogen and the phosphorus content from water and sediments.The studied lakes were found to be eutrophic, Mogosoaia and Pantelimon being in the most advanced state. A significant amount of nitrogen and phosphorus was detected in all sediments analysed, constituting a high accumulation of nutrients from anthropogenic activities. Actions to improve local sanitation infrastructure and to prevent anaerobic leakage of nutrients from sedimentary deposits are recommended.

scholarly journals Evolution of organismal stoichiometry in a long-term experiment with Escherichia coli

2017 ◽  
Vol 4 (7) ◽  
pp. 170497 ◽  
Author(s):  
Caroline B. Turner ◽  
Brian D. Wade ◽  
Justin R. Meyer ◽  
Brooke A. Sommerfeld ◽  
Richard E. Lenski
Keyword(s):  
Escherichia Coli ◽  
Phosphorus Content ◽  
Chemical Elements ◽  
Relative Proportion ◽  
Total Carbon ◽  
Nitrogen And Phosphorus ◽  
Serial Transfer ◽  
Evolutionary Changes ◽  
Long Time ◽  
Long Term Experiment

Organismal stoichiometry refers to the relative proportion of chemical elements in the biomass of organisms, and it can have important effects on ecological interactions from population to ecosystem scales. Although stoichiometry has been studied extensively from an ecological perspective, much less is known about the rates and directions of evolutionary changes in elemental composition. We measured carbon, nitrogen and phosphorus content of 12 Escherichia coli populations that evolved under controlled carbon-limited, serial-transfer conditions for 50 000 generations. The bacteria evolved higher relative nitrogen and phosphorus content, consistent with selection for increased use of the more abundant elements. Total carbon assimilated also increased, indicating more efficient use of the limiting element. We also measured stoichiometry in one population repeatedly through time. Stoichiometry changed more rapidly in early generations than later on, similar to the trajectory seen for competitive fitness. Altogether, our study shows that stoichiometry evolved over long time periods, and that it did so in a predictable direction, given the carbon-limited environment.

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